Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-PgpdA"

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<div id="wrap">
 
<div id="wrap">
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<div class="imageimage1">
 
<div class="imageimage1">
 
<div class="wordword1">
 
<div class="wordword1">
  
<h1>TITLE</h1><hr />
+
<div class="satisfy">
<p>Besides the fungal killing switch and the functional prototype that help reduce concerns over GMO</p>
+
<h1>PgpdA</h1></div>
 +
<div class="satisfysp">
 +
<h1>PgpdA</h1></div>
 +
<hr />
  
</div></div></div>
+
</div></div><br /></div>
  
 
<div class="expand">
 
<div class="expand">
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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/27</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">PgpdA checked with Taq polymerase plasmid PCR</p></li>
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
<div class="technology">Week 2: (1-7 July)</div>
+
<div class="technology">8/3</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
<p>The ordered primers were taken this week.</p>
+
<li><p style="font-size: 18px;">PgpdA checked with KOD polymerase plasmid PCR</p></li>
<p>We would produce our biobricks, but the result was not like we expected after electrophoresis. Then we made colony PCR  and again do it for SOD and GPX. After this, we did digestion and also pTRE vector .</p>
+
<li><p style="font-size: 18px;">PgpdA amplified with KOD polymerase plasmid PCR</p></li>
<img src="https://static.igem.org/mediawiki/2014/7/7e/ATOMS-Notebook1.tif.png">
+
<img src="https://static.igem.org/mediawiki/2014/1/17/ATOMS-Notebook2.png">
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/4</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">PgpdA digested for pBAR_PgpdA construction</p></li>
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
</div>
 
 
<div class="technology">transformation efficiency </div>
 
<div class="thelanguage">
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
 
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
 
</div>
 
</div>
  

Revision as of 23:46, 15 October 2016

PgpdA

PgpdA



7/27
  • PgpdA checked with Taq polymerase plasmid PCR

  • [Collapse]

    8/3
  • PgpdA checked with KOD polymerase plasmid PCR

  • PgpdA amplified with KOD polymerase plasmid PCR

  • [Collapse]

    8/4
  • PgpdA digested for pBAR_PgpdA construction

  • [Collapse]