Difference between revisions of "Team:Northwestern/09 07"

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     <h1>Wednesday, September 7<sup>th</sup></h3>
 
     <h1>Wednesday, September 7<sup>th</sup></h3>
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     <h2>Agenda:</h2>
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  <h2>Transformation Results:</h2>
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    <p>No visible colonies on our Cas9 ligation. The gel screen showed a faint band of the correct size, so we should have seen colonies. We suspect that our transformation efficiency is low.
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      <div class="row">
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        <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/4/46/T--Northwestern--09_07_2.jpg" width="1551" height="1600" alt=""/>
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          <p><strong>Figure 1:</strong> Cas9 ligation</p>
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        </div>
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        <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/3/34/T--Northwestern--09_07_3.jpg" width="1597" height="1600" alt=""/>
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          <p><strong>Figure 2:</strong> Positive Gibson control</p>
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        </div>
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      </div>
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    </p>
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    <div class="row">
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      <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/8/86/T--Northwestern--09_07_4.jpg" width="1551" height="1600" alt=""/>
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        <p><strong>Figure 3:</strong> Transformation positive control</p>
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      </div>
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      <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/5/5a/T--Northwestern--09_07_5.jpg" width="1597" height="1600" alt=""/>
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        <p><strong>Figure 4:</strong> Cas9+ SS Gibson</p>
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      </div>
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    </div>
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    <p>&nbsp; </p>
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     <h2>Tasks:</h2>
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        <p>Michelle</p>
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      <div class="col-sm-10">
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        <ul>
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          <li>Ran gel screen on Cas9 restriction digest</li>
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          <ul>
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            <li>Really faint bands</li>
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            <li>Ligation had 3 bands, were really hard to see</li>
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            <ul>
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              <li>pSB1C3 size, Cas9 size, and one above that </li>
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            </ul>
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          </ul>
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            <div class="col-xs-6 col-sm-7"><img src="https://static.igem.org/mediawiki/2016/8/8b/T--Northwestern--09_07_1.jpg" width="792" height="812" alt=""/></div>
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        </ul>
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      </div>
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    </div>
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    <div class="row">
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      <div class="col-sm-2">
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        <p>Sam</p>
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      </div>
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      <div class="col-sm-10">
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        <ul>
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          <li>Worked on pamphlet</li>
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        </ul>
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      </div>
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    </div>
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  </article>
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Revision as of 02:25, 16 October 2016

Notebook

Wednesday, September 7th

Transformation Results:

No visible colonies on our Cas9 ligation. The gel screen showed a faint band of the correct size, so we should have seen colonies. We suspect that our transformation efficiency is low.

Figure 1: Cas9 ligation

Figure 2: Positive Gibson control

Figure 3: Transformation positive control

Figure 4: Cas9+ SS Gibson

 

Tasks:

Michelle

  • Ran gel screen on Cas9 restriction digest
    • Really faint bands
    • Ligation had 3 bands, were really hard to see
      • pSB1C3 size, Cas9 size, and one above that

Sam

  • Worked on pamphlet