Difference between revisions of "Team:CU-Boulder/Composite Part"
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<h3> Composite Part </h3> | <h3> Composite Part </h3> | ||
<p class = "main"> G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.</p> | <p class = "main"> G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.</p> | ||
| + | <head> | ||
| + | <style> | ||
| + | div.img { | ||
| + | margin: 5px; | ||
| + | border: 2px solid #ccc; | ||
| + | float: left; | ||
| + | width: 600px; | ||
| + | margin-left: 190px; | ||
| + | } | ||
| + | |||
| + | div.img:hover { | ||
| + | border: 2px solid #777; | ||
| + | } | ||
| + | |||
| + | div.img img { | ||
| + | width: 95%; | ||
| + | height: auto; | ||
| + | } | ||
| + | |||
| + | div.desc { | ||
| + | padding: 15px; | ||
| + | text-align: center; | ||
| + | } | ||
| + | </style> | ||
| + | </head> | ||
| + | <body> | ||
| + | |||
| + | <div class="img"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/9/98/TCU-Boulder_EutSpic1.PNG" width="250" height="200" style = "margin-left: 15px; margin-top: 15px"> | ||
| + | </a> | ||
| + | <div class="desc">EutC Tagged eGFP expressed by NEB-5alpha E.Coli</div> | ||
| + | </div> | ||
| + | |||
| + | <div class="img"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/6/6e/TCU-Boulder_EutSpic2.PNG" width="500" height="400" style = "margin-left: 15px; margin-top: 15px"> | ||
| + | </a> | ||
| + | <div class="desc"> EutC tagged eGFP expressed in combination with EutS</div> | ||
| + | </div> | ||
| + | </body> | ||
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Revision as of 03:54, 16 October 2016
Composite Part
G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.
EutC Tagged eGFP expressed by NEB-5alpha E.Coli
EutC tagged eGFP expressed in combination with EutS
