Difference between revisions of "Team:CU-Boulder/Composite Part"

Revision as of 04:01, 16 October 2016

Composite Part

G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.

Mutant G26-Amber EutS with no available AzoPhe

Mutant G26-Amber EutS with .1% AzoPhe

Mutant G26-Amber EutS with .1% AzoPhe

@@ Line 97: / Line 97: @@
      <img src="https://static.igem.org/mediawiki/2016/c/c1/TCU-Boulder_Comp1.PNG" width="250" height="200" style = "margin-left: 15px; margin-top: 15px">
    </a>
-   <div class="desc">Mutant: G26-Amber EutS with no available AzoPhe</div>
+   <div class="desc">Mutant G26-Amber EutS with no available AzoPhe</div>
 </div>
@@ Line 103: / Line 103: @@
      <img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="500" height="400" style = "margin-left: 15px; margin-top: 15px">
    </a>
-   <div class="desc"> Mutant: G26-Amber EutS with .1% AzoPhe</div>
+   <div class="desc"> Mutant G26-Amber EutS with .1% AzoPhe</div>
 </div>
+<div class="img">
+    <img src="https://static.igem.org/mediawiki/2016/2/21/TCU-Boulder_Comp2.PNG" width="500" height="400" style = "margin-left: 15px; margin-top: 15px">
+  </a>
+  <div class="desc"> Mutant G26-Amber EutS with .1% AzoPhe</div>
+</div>
 </body>