Difference between revisions of "Team:CU-Boulder/Composite Part"
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<img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px"> | <img src="https://static.igem.org/mediawiki/2016/f/fd/TCU-1c3plasmid.PNG" width="712" height="645" style = "margin-left: 15px; margin-top: 15px"> | ||
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<div class="desc"> 1C3 Plasmid map for G26-Amber EutS</div> | <div class="desc"> 1C3 Plasmid map for G26-Amber EutS</div> | ||
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Revision as of 04:17, 16 October 2016
Composite Part
G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.
Mutant G26-Amber EutS with no available AzoPhe
Mutant G26-Amber EutS with .1% AzoPhe
1C3 Plasmid map for G26-Amber EutS
