Difference between revisions of "Team:Northwestern/09 16"

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     <h1>Friday, September 16<sup>th</sup></h3>
 
     <h1>Friday, September 16<sup>th</sup></h3>
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     <h2>Agenda:</h2>
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     <h2>Tasks:</h2>
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+
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Jordan</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Went to Auburn Gresham for outreach</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Went to Auburn Gresham for outreach</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Presented at Auburn Gresham</li>
 +
          <li>Collected 8 primers from Leonard lab office</li>
 +
          <li>Ran PCR for linearization of pET28a plasmid miniprep (one 50-μL reaction)</li>
 +
          <ul>
 +
            <li>0.5 μL pet28 miniprep (23 ng/μL) </li>
 +
            <li>2.5 μL 10μM Lnrz_pET28a_FWD </li>
 +
            <li>2.5 μL 10 μM Lnrz_pET28a_REV </li>
 +
            <li>1 μL DMSO </li>
 +
            <li>18.5 μL nuclease-free water </li>
 +
            <li>25 μL Q5 HiFi 2X Master Mix </li>
 +
            <div class="row">
 +
              <div class="col-xs-6 col-sm-8"><img src="https://static.igem.org/mediawiki/2016/3/33/T--Northwestern--09_16_1.png" width="1006" height="260" alt=""/></div>
 +
            </div>
 +
          </ul>
 +
          <li>Ran PCR for amplifying Cas9 to insert to Pet28 backbone (one 50-μL reaction) </li>
 +
          <ul>
 +
            <li>1 μL Cas9 1:3 template </li>
 +
            <li>1 μL 10 μM Cas9_pET28a_insertFWD </li>
 +
            <li>1 μL 10 μM Cas9-pET28a_insertREV </li>
 +
            <li>1 μL DMSO </li>
 +
            <li>21 μL nuclease-free water </li>
 +
            <li>25 μL OneTaq 2X Master Mix </li>
 +
            <div class="row">
 +
              <div class="col-xs-6 col-sm-8"><img src="https://static.igem.org/mediawiki/2016/8/83/T--Northwestern--09_16_2.png" width="1006" height="260" alt=""/></div>
 +
            </div>
 +
          </ul>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Went to Auburn Gresham for outreach</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
  </article>
 
<footer id="nav">
 
<footer id="nav">
 
       <div class="row">
 
       <div class="row">
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       </div>
 
     </footer>
 
     </footer>
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  </article>
 
 
<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>
 
         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>

Revision as of 04:18, 16 October 2016

Notebook

Friday, September 16th

Tasks:

Jordan

  • Went to Auburn Gresham for outreach

Sara

  • Went to Auburn Gresham for outreach

Tasfia

  • Presented at Auburn Gresham
  • Collected 8 primers from Leonard lab office
  • Ran PCR for linearization of pET28a plasmid miniprep (one 50-μL reaction)
    • 0.5 μL pet28 miniprep (23 ng/μL)
    • 2.5 μL 10μM Lnrz_pET28a_FWD
    • 2.5 μL 10 μM Lnrz_pET28a_REV
    • 1 μL DMSO
    • 18.5 μL nuclease-free water
    • 25 μL Q5 HiFi 2X Master Mix
  • Ran PCR for amplifying Cas9 to insert to Pet28 backbone (one 50-μL reaction)
    • 1 μL Cas9 1:3 template
    • 1 μL 10 μM Cas9_pET28a_insertFWD
    • 1 μL 10 μM Cas9-pET28a_insertREV
    • 1 μL DMSO
    • 21 μL nuclease-free water
    • 25 μL OneTaq 2X Master Mix

Tyler

  • Went to Auburn Gresham for outreach