Difference between revisions of "Team:Northwestern/09 22"

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     <h1>Thursday, September 22<sup>nd</sup></h3>
 
     <h1>Thursday, September 22<sup>nd</sup></h3>
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    <h2>Agenda:</h2>
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  <h2>Tasks:</h2>
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        <p>Jordan</p>
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        <ul>
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          <li>Checked new overnight culture from the one plate with lots of colonies</li>
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          <ul>
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            <li>Still didn’t grow much</li>
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            <li>Made a new overnight culture from the other plate once the colony is big enough</li>
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          </ul>
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          <li>Miniprepped pET28a-Cas9 and pET28a-Cas9-SS Gibson cultures (leave 500uL culture for glycerol stocks)</li>
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          <ul>
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            <li>Accidentally used all of the culture</li>
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          </ul>
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          <li>Miniprepped pSB1C3-Cas9 ligation cultures (leave 500uL culture for glycerol stocks)</li>
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          <ul>
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            <li>Accidentally used all of the culture</li>
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          </ul>
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        </ul>
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      </div>
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        <p>Tasfia</p>
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        <ul>
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          <li>Nanodropped minipreps
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              <div class="col-xs-10"><img src="https://static.igem.org/mediawiki/2016/5/5c/T--Northwestern--09_22_1.png" width="906" height="1116" alt=""/></div>
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          </li>
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        </ul>
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<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
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Revision as of 04:53, 16 October 2016

Notebook

Thursday, September 22nd

Tasks:

Jordan

  • Checked new overnight culture from the one plate with lots of colonies
    • Still didn’t grow much
    • Made a new overnight culture from the other plate once the colony is big enough
  • Miniprepped pET28a-Cas9 and pET28a-Cas9-SS Gibson cultures (leave 500uL culture for glycerol stocks)
    • Accidentally used all of the culture
  • Miniprepped pSB1C3-Cas9 ligation cultures (leave 500uL culture for glycerol stocks)
    • Accidentally used all of the culture

Tasfia

  • Nanodropped minipreps