Difference between revisions of "Team:Northwestern/10 05"

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     <h1>Wednesday, October 5<sup>th</sup></h3>
 
     <h1>Wednesday, October 5<sup>th</sup></h3>
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     <h2>Agenda:</h2>
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     <h2>Tasks:</h2>
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        <p>Jordan</p>
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          <li>Grow up cultures to innoculate Western blot cultures (2 mL)</li>
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          <ul><li>JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP</li>
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          <li>BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9</li></ul>
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          <li>Overnight cultures for pSB1C3-gRNA-template &amp; pSB1C3-gRNA-guide (Top10)</li>
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    <p>Paul</p>
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      <li>Send pSB1C3-gRNA-template &amp; pSB1C3-gRNA-guide for sequencing</li>
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Revision as of 05:25, 16 October 2016

Notebook

Wednesday, October 5th

Tasks:

Jordan

  • Grow up cultures to innoculate Western blot cultures (2 mL)
    • JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
    • BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9
  • Overnight cultures for pSB1C3-gRNA-template & pSB1C3-gRNA-guide (Top10)

Paul

  • Send pSB1C3-gRNA-template & pSB1C3-gRNA-guide for sequencing