Difference between revisions of "Team:Northeastern/Basic Part"

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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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                <h3>basic parts</h3>
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                <h4>NADH Oxidase</h4>
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                    <p>This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:</p>
  
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                    <p style="text-align:center;">4 NADH + O<sub>2</sub> → 4 NAD<sup>+</sup> + 2 H<sub>2</sub>O + 4 e<sup>-</sup></p>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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                    <p>We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and modified RBS sequences based on the one submitted with the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1155008">fnr coding sequence by Paris Saclay 2013</a> optimized to reduce homodimer formation during PCR (<a href="http://parts.igem.org/Part:BBa_K2182001">BBa_K2182004</a> and <a  href="/">BBa_K2182005</a>).</p>
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                        <a href="/">Summary</a>
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                        <a href="/">Composite</a>
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                        <a href="/">Collection</a>
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            <a href="http://www.northeastern.edu/">Northeastern University, Boston MA</a> &middot;
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            <a href="https://github.com/omwan/iGEM-wiki-neu16">Github</a>
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Revision as of 21:01, 16 October 2016

basic parts

NADH Oxidase

This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:

4 NADH + O2 → 4 NAD+ + 2 H2O + 4 e-

We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and modified RBS sequences based on the one submitted with the fnr coding sequence by Paris Saclay 2013 optimized to reduce homodimer formation during PCR (BBa_K2182004 and BBa_K2182005).