Difference between revisions of "Team:Northeastern/Basic Part"

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Revision as of 21:12, 16 October 2016

basic parts

NADH Oxidase

This is a novel part submitted to the registry from the NCBI database, NADH oxidase/NADH dehydrogenase from Streptococcus sanguinis. Nox encodes the H2O-forming NADH oxidase enzyme that catalyses the oxidation of NADH to form water:

4 NADH + O2 → 4 NAD+ + 2 H2O + 4 e-

We also designed a Nox protein with an N-terminus His tag to purify the protein using ELISA (BBa_K2182001), and modified RBS sequences based on the one submitted with the fnr coding sequence by Paris Saclay 2013 optimized to reduce homodimer formation during PCR (BBa_K2182004 and BBa_K2182005).