Difference between revisions of "Team:Northeastern/Proof"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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                <h3>Proof of Concept</h3>
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                    <p>To determine the potential of our parts to improve microbial electrolysis, we first performed <i>in vivo</i> tests for the ability of PR+blh to lower pH and of Nox to reduce oxygen levels. &nbsp;</p><p>The pH of liquid cultures of cells containing the K2182002 was compared in dark and light conditions, and with and without B-carotene needed for PR function. The pH of cells grown with both light and B-carotene rose more slowly than other cultures, suggesting that the plasmid could be used to moderate the alkalinity of an MEC cathode and provide protons for hydrogen production. This is in agreement with previous characterization performed by UNITN-Trento 2015.</p>
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                    <p> The dissolved oxygen concentration of liquid cultures of <i>E. coli </i>BL21 was compared for wild type cells and those transformed with nox plasmid K3282003. The presence of the nox plasmid lowered the dissolved oxygen concentration over time in cultures with the same number of cells exposed to air.</p>
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                        <a href="/Team:Northeastern/Description">Description</a>
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                        <a href="/Team:Northeastern/Design">Design</a>
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                        <a href="/Team:Northeastern/Experiments">Experiments</a>
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                        <a href="/Team:Northeastern/Results">Results</a>
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
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<h4> What should we do for our proof of concept? </h4>
 
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
 
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            <a href="http://www.northeastern.edu/">Northeastern University, Boston MA</a> &middot;
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            <a href="https://github.com/omwan/iGEM-wiki-neu16">Github</a>
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Latest revision as of 23:52, 16 October 2016

Proof of Concept

To determine the potential of our parts to improve microbial electrolysis, we first performed in vivo tests for the ability of PR+blh to lower pH and of Nox to reduce oxygen levels.  

The pH of liquid cultures of cells containing the K2182002 was compared in dark and light conditions, and with and without B-carotene needed for PR function. The pH of cells grown with both light and B-carotene rose more slowly than other cultures, suggesting that the plasmid could be used to moderate the alkalinity of an MEC cathode and provide protons for hydrogen production. This is in agreement with previous characterization performed by UNITN-Trento 2015.

The dissolved oxygen concentration of liquid cultures of E. coli BL21 was compared for wild type cells and those transformed with nox plasmid K3282003. The presence of the nox plasmid lowered the dissolved oxygen concentration over time in cultures with the same number of cells exposed to air.