Difference between revisions of "Team:BGIC China"

Revision as of 07:22, 2 July 2016

iGEM BGI College

BGI-College China
Project
Open source Cell-free system based test paper platform
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Team
About our team
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Human Practice
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Safety
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Blog
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1. Project
1.1 Project Description
1.2 Target
1.2.1 Plasmids and standard parts registry
1.2.2 Laboratory and clinical sample tests
1.2.3 To design, test and, if possible, manufacture test paper reader
1.2.4 Pearl necklace-shaped test paper effectiveness assessment
1.3 Detailed description
1.3.1 Abstract
1.3.2 Design
1.3.2.1 Plasmid Design
1.3.2.2 The application of cell-free system
1.3.2.3 Pearl necklace-shaped test paper design
1.3.3 Methods
1.3.3.1 The application of S30 T7 System
1.3.3.2 Fragment ampliﬁcation and plasmid assembly
1.3.3.3 Creating Estradiol Receptor (E2R) and Progesterone Receptor (PR)
1.3.3.4 The testing of cell-free system reaction on a paper disc
1.3.4 Modelling
1.4 Our results
1.4.1 BioBricks and part collections
1.4.2 Graphs plotted from ampliﬁcation tests
1.5 Lab summary
1.6 Lab Journal
1.7 Intellectual Property
1.1 Project Description
As the first high school team of BGI-college, we endeavour to construct a paper-based cell-free system with whose function is to measure the concentration of target molecules in the sample within a certain range. What matters the most is that this attempt can be attributed into the application of bionics as the transcription and translation devices of the original cell is still present in the cell smear and thus a testing platform for a variety of molecules is created, and hereby being relieved of comprehensive limitations of using living cells for testing. This test paper integrated with biosensors for target molecules enabled the testing of the concentration of a variety of target molecules(various hormones, glucose, fatty acid or nitrates,etc.) in various clinical samples of plasma, urine or saliva. Quantitative results can be shown by the output of a variety of proteins.(light emission of GFP or the changing of colour initiated by LacZ,etc.). The high portability of the test paper and the small duration of the testing process of clinical samples, should the test paper be successfully manufactured, shall relieve millions of patients of comprehensive time-consuming and perhaps agonising medical inspections—these could be carried out at home! The test paper system, presumably, transcends mainstream medical inspections of clinical samples in means of efficiency and convenience.

Our idea originated from comprehensive discussions that eliminated the alternatives of acetochlor test papers which already exists in the market at a feasible price and the cholinesterase injection plan for protection of nerve system against Sarin gas as the project relied upon the injected medicine’s access through the Brain-Blood Barriers——an unsolvable dilemma. The idea of cell-free test paper system came up before we came across two articles that outlined the feasibility of a paper-based cell-free system for inspection of clinical samples targeting at specific molecules(1)(2). With the solid theoretical foundation and overwhelming feasibility compared to the other ideas listed above, the cell-free test paper system was chosen by the team unanimously. In late April, the designing of our plasmids was initiated via comprehensive methods, which, upon finishing even as we post this passage, has gone through three phases. Upon the biosensor’s contact with the target molecule, the genetic circuit will produce an output with the fluorescence of a GFP.

Our goals go as follows. The very foundation of our targets is to apply our cell-free system, the system of cell environment simulation which consists of cell-extract and substrate that enables DNA expression in vitro to a mature test paper module. Laboratory sample and clinical sample tests will be carried out to assess the effectiveness of the coalition. To make that happen, we will attempt on testing the feasibility of our test paper prototype in pursuit of building up a semi-quantitative test-paper system. Constant amendments, adjustments and improvements to the laboratory tasks specified in the original plans and/or to the plans themselves may be made for the better in accordance with the outcome of the attempt of putting theoretical research and arrangements into practice.

1. A Courbet, D Endy, E Renard, F Molina, Jérôme Bonnet, Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Science Translational Medicine, Vol. 7, Issue 289, pp. 289ra83 (2015)
2. Keith Pardee, Alexander A. Green, Tom Ferrante, D. Ewen Cameron, Ajay DaleyKeyser, Peng Yin, James J. Collins, Paper-Based Synthetic Gene Networks. Cell 159, 940–954 (2014)
1.2 Target
(Blank)
1.3 Detailed description
(Blank)
(Blank)
(Blank)
1.4 Our results
(Blank)
1.5 Lab summary
(Blank)
1.6 Lab Journal
(Blank)
1.7 Intellectual Property
(Blank)
2. Team
2.1 Meet the team
2.2 Team member intro
2.3 Group pic
2.1 Meet the team
(Blank)
2.2 Team member intro
(Blank)
2.3 Group pic
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3. Human practice
3.1Sponsorship& Attribution
(Blank)
4. Safety
4.1 Laboratory safety assessment
4.1.2 Materials used
4.1.3 Protection
4.1.4 Disposal
4.2 Identiﬁed possible risks of our project
4.2.1 Risks to the safety and health of team members, or other people working in the lab
4.2.2 Risks to the safety and health of the general public (if any biological materials escaped from our lab)
4.2.3 Risks to the environment (from waste disposal, or from materials escaping from our lab)
4.2.4 Risks to security through malicious misuse by individuals, groups, or countries
4.2.5 What new risks might arise from our project's growth?
4.1 Laboratory safety assessment
4.1.1 Materials used

(Blank)

4.1.2 Protection

(Blank)

4.1.3 Disposal

(Blank)
4.2 Identiﬁed possible risks of our project
4.2.1 Risks to the safety and health of team members, or other people working in the lab

(Blank)

4.2.2 Risks to the safety and health of the general public (if any biological materials escaped from our lab)

(Blank)

4.2.3 Risks to the environment (from waste disposal, or from materials escaping from our lab)

(Blank)

4.2.4 Risks to security through malicious misuse by individuals, groups, or countries

(Blank)

4.2.5 What new risks might arise from our project's growth?

(Blank)
5. Blog
Feel free to subscribe our blog!

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Project\u003c/p\u003e","backupValue":"","version":1},"text2":{"type":"RichText","id":"f_75be05f1-c34d-43cb-a610-0707dd27cd82","defaultValue":false,"value":"\u003cp style=\"font-size:80%\"\u003e\u003ca href=\"#1-1-project-description\"\u003e1.1 Project Description\u003c/a\u003e\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e\u003ca href=\"#1-2-target\"\u003e1.2 Target\u003c/a\u003e\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.2.1 Plasmids and standard parts registry\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.2.2 Laboratory and clinical sample tests\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.2.3 To design, test and, if possible, manufacture test paper reader\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.2.4 Pearl necklace-shaped test paper effectiveness assessment\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e\u003ca href=\"#1-3-detailed-description\"\u003e1.3 Detailed description\u003c/a\u003e\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.1 Abstract\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.2 Design\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.2.1 Plasmid Design\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.2.2 The application of cell-free system\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.2.3 Pearl necklace-shaped test paper design\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.3 Methods\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.3.1 The application of S30 T7 System\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.3.2 Fragment ampliﬁcation and plasmid assembly\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.3.3 Creating Estradiol Receptor (E2R) and Progesterone Receptor (PR)\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.3.4 The testing of cell-free system reaction on a paper disc\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.3.4 Modelling\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e\u003ca href=\"#\"\u003e1.4 Our results\u003c/a\u003e\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.4.1 BioBricks and part collections\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e1.4.2 Graphs plotted from ampliﬁcation tests\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e\u003ca href=\"#\"\u003e1.5 Lab summary\u003c/a\u003e\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e\u003ca href=\"#\"\u003e1.6 Lab Journal\u003c/a\u003e\u003c/p\u003e\u003cp style=\"font-size:80%\"\u003e\u003ca href=\"#\"\u003e1.7 Intellectual Property \u003c/a\u003e\u003c/p\u003e","backupValue":"","version":1},"block1":{"type":"BlockComponent","id":"f_361f862b-4f7a-4d9a-a4fe-dfda6ffa40e0","defaultValue":null,"items":[]}}},{"type":"Slide","id":"f_e8e055b9-1c4b-44e2-906e-3040c497c221","defaultValue":null,"template_id":null,"template_name":"block","components":{"slideSettings":{"type":"SlideSettings","id":"f_17d7e2c5-d678-47a1-811e-9aaaba95ab1e","defaultValue":null,"show_nav":false,"nameChanged":true,"name":"1.1 Project Description","sync_key":null,"layout_variation":null},"background1":{"type":"Background","id":"f_acadc1cf-0898-4e56-8079-fdf73fb84af1","defaultValue":false,"url":"http://uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg","textColor":"light","backgroundVariation":"","sizing":"tile","videoUrl":"","videoHtml":"","storageKey":null,"storage":null,"format":null,"h":900,"w":1600,"s":null},"text1":{"type":"RichText","id":"f_626617cb-9345-479a-8c95-78d5e1472b1e","defaultValue":false,"value":"","backupValue":"","version":1},"text2":{"type":"RichText","id":"f_7019b4e0-cfec-463c-a5a0-deba48c1ee95","defaultValue":false,"value":"\u003cp\u003e1.1 Project Description\u003c/p\u003e","backupValue":"","version":1},"block1":{"type":"BlockComponent","id":"f_16ee6dcf-2e3f-4e3f-8596-3bae6cfc6652","defaultValue":null,"items":[{"type":"RichText","id":"f_e24a28d0-204a-4dd5-86ec-1ca9d73fc887","defaultValue":false,"value":"\u003cp style=\"text-align: left;\"\u003eAs the first high school team of BGI-college, we endeavour to construct a paper-based cell-free system with whose function is to measure the concentration of target molecules in the sample within a certain range. What matters the most is that this attempt can be attributed into the application of bionics as the transcription and translation devices of the original cell is still present in the cell smear and thus a testing platform for a variety of molecules is created, and hereby being relieved of comprehensive limitations of using living cells for testing. This test paper integrated with biosensors for target molecules enabled the testing of the concentration of a variety of target molecules(various hormones, glucose, fatty acid or nitrates,etc.) in various clinical samples of plasma, urine or saliva. Quantitative results can be shown by the output of a variety of proteins.(light emission of GFP or the changing of colour initiated by LacZ,etc.). The high portability of the test paper and the small duration of the testing process of clinical samples, should the test paper be successfully manufactured, shall relieve millions of patients of comprehensive time-consuming and perhaps agonising medical inspections—these could be carried out at home! The test paper system, presumably, transcends mainstream medical inspections of clinical samples in means of efficiency and convenience.\u003c/p\u003e\u003cp style=\"text-align: left;\"\u003e \u003c/p\u003e\u003cp style=\"text-align: left;\"\u003eOur idea originated from comprehensive discussions that eliminated the alternatives of acetochlor test papers which already exists in the market at a feasible price and the cholinesterase injection plan for protection of nerve system against Sarin gas as the project relied upon the injected medicine’s access through the Brain-Blood Barriers——an unsolvable dilemma. The idea of cell-free test paper system came up before we came across two articles that outlined the feasibility of a paper-based cell-free system for inspection of clinical samples targeting at specific molecules(1)(2). With the solid theoretical foundation and overwhelming feasibility compared to the other ideas listed above, the cell-free test paper system was chosen by the team unanimously. In late April, the designing of our plasmids was initiated via comprehensive methods, which, upon finishing even as we post this passage, has gone through three phases. Upon the biosensor’s contact with the target molecule, the genetic circuit will produce an output with the fluorescence of a GFP.\u003c/p\u003e\u003cp style=\"text-align: left;\"\u003e \u003c/p\u003e\u003cp style=\"text-align: left;\"\u003eOur goals go as follows. 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 </script> </head> <body class='show locale-en' id='sites'> <div id="s-page-container"><div id="s-content" class="s-variation-default s-entrance-mask s-font-heading-archivo-narrow s-font-title-archivo-narrow s-font-body-default" data-reactroot="" data-reactid="1" data-react-checksum="-1908358507"><div class="social-thumbnail" data-reactid="2"><div style="display:none;" data-reactid="3"><img src="https://res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_630,w_1200/v1/791914/IGEM_logo_2500x2500_xdwzta.png" alt="page thumbnail" width="300" height="300" data-reactid="4"/></div><div id="lw-share-data" style="display:none;" data-reactid="5"><div class="lws-title" data-reactid="6">iGEM BGI College</div><div class="lws-description" data-reactid="7">Website</div><div class="lws-image" data-reactid="8">https://res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_630,w_1200/v1/791914/IGEM_logo_2500x2500_xdwzta.png</div></div></div><!-- react-empty: 9 --><div class="s-navbar-section navigator fixed" id="s-nav" data-reactid="10"><ul class="s-nav" data-reactid="11"><div class="ib logo" data-reactid="12"><div class="s-component s-image" data-reactid="13"><span data-reactid="14"><div class="s-component-content" data-reactid="15"><div data-reactid="16"><!-- react-text: 17 --> <!-- /react-text --><img src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_300,w_300,f_auto,q_90/v1/791914/IGEM_logo_2500x2500_xdwzta.png" alt="" title="" data-description="" data-reactid="18"/><!-- react-text: 19 --> <!-- /react-text --></div></div></span></div></div><li style="display:none;" data-reactid="20"><a class="s-font-body s-nav-item" href="#intro" data-reactid="21">BGI-College China</a></li><li style="display:none;" data-reactid="22"><a class="s-font-body s-nav-item" href="#how-it-works" data-reactid="23">滑块</a></li><li data-reactid="24"><a class="s-font-body s-nav-item" href="#feature-listing" data-reactid="25">1. Project</a></li><li style="display:none;" data-reactid="26"><a class="s-font-body s-nav-item" href="#figures" data-reactid="27">1.1 Project Description</a></li><li style="display:none;" data-reactid="28"><a class="s-font-body s-nav-item" href="#contact-us" data-reactid="29">1.2 Target</a></li><li style="display:none;" data-reactid="30"><a class="s-font-body s-nav-item" href="#become-partner" data-reactid="31">1.3 Detailed description </a></li><li style="display:none;" data-reactid="32"><a class="s-font-body s-nav-item" href="#blog" data-reactid="33">1.4 Our results </a></li><li style="display:none;" data-reactid="34"><a class="s-font-body s-nav-item" data-reactid="35">1.5 Lab summary </a></li><li style="display:none;" data-reactid="36"><a class="s-font-body s-nav-item" data-reactid="37">1.6 Lab Journal </a></li><li style="display:none;" data-reactid="38"><a class="s-font-body s-nav-item" data-reactid="39">1.7 Intellectual Property </a></li><li data-reactid="40"><a class="s-font-body s-nav-item" data-reactid="41">2. Team</a></li><li style="display:none;" data-reactid="42"><a class="s-font-body s-nav-item" data-reactid="43">2.1 Meet the team</a></li><li style="display:none;" data-reactid="44"><a class="s-font-body s-nav-item" data-reactid="45">2.2 Team member intro </a></li><li style="display:none;" data-reactid="46"><a class="s-font-body s-nav-item" data-reactid="47">2.3 Group pic </a></li><li data-reactid="48"><a class="s-font-body s-nav-item" data-reactid="49">3. Human practice</a></li><li data-reactid="50"><a class="s-font-body s-nav-item" data-reactid="51">4. Safety</a></li><li style="display:none;" data-reactid="52"><a class="s-font-body s-nav-item" data-reactid="53">4.1 Laboratory safety assessment</a></li><li style="display:none;" data-reactid="54"><a class="s-font-body s-nav-item" data-reactid="55">4.2 Identiﬁed possible risks of our project</a></li><li data-reactid="56"><a class="s-font-body s-nav-item" data-reactid="57">5. Blog</a></li><li style="display:none;" data-reactid="58"><a class="s-font-body s-nav-item" data-reactid="59">博客</a></li><li id="s-ecommerce-nav-shopping-cart-wrapper" class="s-ecommerce-nav-shopping-cart-wrapper hidden" data-reactid="60"></li></ul></div><!-- react-empty: 61 --><ul class="slides s-page-1" data-reactid="62"><li class="slide" id="section-f_d86296d0-17d4-44e3-99b4-34d5c6b66938" data-reactid="63"><div class="waypoint" data-reactid="64"></div><a class="section-anchor" data-reactid="65"></a><div class="s-bg-image s-bg-dark-text s-section s-title-section" style="background-repeat:no-repeat;background-size:cover;background-color:transparent;background-position:50% 50%;background-image:url(//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1500,w_2000,f_auto/v1/791914/1383706903_fqcrpr.jpg);" data-reactid="66"><div class="container" data-reactid="67"><div class="columns sixteen" data-reactid="68"><div class="s-title-group " data-reactid="69"><div data-reactid="70"><div 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class="s-subtitle" data-reactid="108"><div class="s-component s-text" data-reactid="109"><h4 class="s-component-content s-font-heading" data-reactid="110"><p class="s-text-color-white">Open source Cell-free system based test paper platform</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="111"><div class="" data-reactid="112"><div class="s-button s-component" data-reactid="113"><span data-reactid="114"><div class="s-component-content" data-reactid="115"><a class="s-action-button s-common-button s-font-body" href="#2" data-component="button" target="_self" data-reactid="116">Read More</a></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-dark-text item" style="background-repeat:no-repeat;background-size:cover;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//assets.strikingly.com/static/backgrounds/blur/6.jpg" data-reactid="117"><div class="inner" data-reactid="118"><div class="container right s-rva" data-reactid="119"><div class="eight columns right media-outer s-rva-media" data-reactid="120"><div class="media-wrapper" data-reactid="121"><div class="s-component s-media " data-reactid="122"><span data-reactid="123"><div class="s-component-content" data-reactid="124"><div data-reactid="125"><!-- react-text: 126 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:766px;max-height:369px;" data-reactid="127"><div class="s-ratio-fill" style="padding-bottom:48.17%;" data-reactid="128"></div><div data-reactid="129"><img alt="" title="" data-description="" width="766" height="369" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/1383706903_fqcrpr.jpg" data-sizes="auto" data-reactid="130"/><noscript data-reactid="131"><img alt="" title="" data-description="" width="766" height="369" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/1383706903_fqcrpr.jpg" class="" data-reactid="132"/></noscript></div></div><!-- react-text: 133 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="134"><div class="half-offset-right" data-reactid="135"><div class="s-title-group" data-reactid="136"><div class="s-title " data-reactid="137"><div class="s-component s-text" data-reactid="138"><h2 class="s-component-content s-font-title" data-reactid="139"><p class="s-text-color-white">Team</p></h2></div></div><div class="s-subtitle" data-reactid="140"><div class="s-component s-text" data-reactid="141"><h4 class="s-component-content s-font-heading" data-reactid="142"><p class="s-text-color-white">About our team</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="143"><div class="" data-reactid="144"><div class="s-button s-component" data-reactid="145"><span data-reactid="146"><div class="s-component-content" data-reactid="147"><a class="s-action-button s-common-button s-font-body" href="#2" data-component="button" target="_self" data-reactid="148">Read More</a></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-dark-text item" style="background-repeat:no-repeat;background-size:cover;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//assets.strikingly.com/static/backgrounds/blur/4.jpg" data-reactid="149"><div class="inner" data-reactid="150"><div class="container right s-rva" data-reactid="151"><div class="eight columns right media-outer s-rva-media" data-reactid="152"><div class="media-wrapper" data-reactid="153"><div class="s-component s-media " data-reactid="154"><span data-reactid="155"><div class="s-component-content" data-reactid="156"><div data-reactid="157"><!-- react-text: 158 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:766px;max-height:369px;" data-reactid="159"><div class="s-ratio-fill" style="padding-bottom:48.17%;" data-reactid="160"></div><div data-reactid="161"><img alt="" title="" data-description="" width="766" height="369" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/1383706903_fqcrpr.jpg" data-sizes="auto" data-reactid="162"/><noscript data-reactid="163"><img alt="" title="" data-description="" width="766" height="369" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/1383706903_fqcrpr.jpg" class="" data-reactid="164"/></noscript></div></div><!-- react-text: 165 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="166"><div class="half-offset-right" data-reactid="167"><div class="s-title-group" data-reactid="168"><div class="s-title " data-reactid="169"><div class="s-component s-text" data-reactid="170"><h2 class="s-component-content s-font-title" data-reactid="171"><p class="s-text-color-white">Human Practice</p></h2></div></div><div class="s-subtitle" data-reactid="172"><div class="s-component s-text" data-reactid="173"></div></div></div><div class="cta-button-wrapper" data-reactid="174"><div class="" data-reactid="175"><div class="s-button s-component" data-reactid="176"><span data-reactid="177"><div class="s-component-content" data-reactid="178"><a class="s-action-button s-common-button s-font-body" href="#2" data-component="button" target="_self" data-reactid="179">Read More</a></div></span></div></div></div></div></div></div></div></div><div class="lazyload 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class="s-subtitle" data-reactid="203"><div class="s-component s-text" data-reactid="204"></div></div></div><div class="cta-button-wrapper" data-reactid="205"><div class="" data-reactid="206"><div class="s-button s-component" data-reactid="207"><span data-reactid="208"><div class="s-component-content" data-reactid="209"><a class="s-action-button s-common-button s-font-body" href="#2" data-component="button" target="_self" data-reactid="210">Read More</a></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-dark-text item" style="background-repeat:no-repeat;background-size:cover;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//assets.strikingly.com/static/backgrounds/blur/4.jpg" data-reactid="211"><div class="inner" data-reactid="212"><div class="container right s-rva" data-reactid="213"><div class="eight columns right 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s-common-button s-font-body" href="#2" data-component="button" target="_self" data-reactid="241">Read More</a></div></span></div></div></div></div></div></div></div></div></div><div class="center slide-selectors" data-reactid="242"><div class="slide-selectors-inner" data-reactid="243"><div class="content repeatable-component" data-reactid="244"><div class="selector-wrapper" data-reactid="245"><div class="selector" data-reactid="246"></div></div><div class="selector-wrapper" data-reactid="247"><div class="selector" data-reactid="248"></div></div><div class="selector-wrapper" data-reactid="249"><div class="selector" data-reactid="250"></div></div><div class="selector-wrapper" data-reactid="251"><div class="selector" data-reactid="252"></div></div><div class="selector-wrapper" data-reactid="253"><div class="selector" data-reactid="254"></div></div><div class="clearfix" data-reactid="255"></div></div></div></div><div class="prev-button" data-reactid="256"><div class="arrow" data-reactid="257"></div></div><div class="next-button" data-reactid="258"><div class="arrow" data-reactid="259"></div></div></div></div></div></div></div></li><li class="slide" id="section-f_af65170e-e964-45f2-a2d9-9420e99a9e3b" data-reactid="260"><div class="waypoint" data-reactid="261"></div><a class="section-anchor" data-reactid="262"></a><div class="lazyload s-no-bg s-block-section s-section" data-reactid="263"><div class="container" data-reactid="264"><div class="columns sixteen" data-reactid="265"><div class="s-title-group " data-reactid="266"><div data-reactid="267"><div class="s-title " data-reactid="268"><div class="s-component s-text" data-reactid="269"><h2 class="s-component-content s-font-title" data-reactid="270"><p>1. Project</p></h2></div></div><div class="s-subtitle" data-reactid="271"><div class="s-component s-text" data-reactid="272"><h4 class="s-component-content s-font-heading" data-reactid="273"><p style="font-size:80%"><a href="#1-1-project-description">1.1 Project Description</a></p><p style="font-size:80%"><a href="#1-2-target">1.2 Target</a></p><p style="font-size:80%">1.2.1 Plasmids and standard parts registry</p><p style="font-size:80%">1.2.2 Laboratory and clinical sample tests</p><p style="font-size:80%">1.2.3 To design, test and, if possible, manufacture test paper reader</p><p style="font-size:80%">1.2.4 Pearl necklace-shaped test paper effectiveness assessment</p><p style="font-size:80%"><a href="#1-3-detailed-description">1.3 Detailed description</a></p><p style="font-size:80%">1.3.1 Abstract</p><p style="font-size:80%">1.3.2 Design</p><p style="font-size:80%">1.3.2.1 Plasmid Design</p><p style="font-size:80%">1.3.2.2 The application of cell-free system</p><p style="font-size:80%">1.3.2.3 Pearl necklace-shaped test paper design</p><p style="font-size:80%">1.3.3 Methods</p><p style="font-size:80%">1.3.3.1 The application of S30 T7 System</p><p style="font-size:80%">1.3.3.2 Fragment ampliﬁcation and plasmid assembly</p><p style="font-size:80%">1.3.3.3 Creating Estradiol Receptor (E2R) and Progesterone Receptor (PR)</p><p style="font-size:80%">1.3.3.4 The testing of cell-free system reaction on a paper disc</p><p style="font-size:80%">1.3.4 Modelling</p><p style="font-size:80%"><a href="#">1.4 Our results</a></p><p style="font-size:80%">1.4.1 BioBricks and part collections</p><p style="font-size:80%">1.4.2 Graphs plotted from ampliﬁcation tests</p><p style="font-size:80%"><a href="#">1.5 Lab summary</a></p><p style="font-size:80%"><a href="#">1.6 Lab Journal</a></p><p style="font-size:80%"><a href="#">1.7 Intellectual Property </a></p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="274"><div class="s-repeatable s-block s-component s-mh " data-reactid="275"></div></div></div></div></li><li class="slide" id="section-f_e8e055b9-1c4b-44e2-906e-3040c497c221" data-reactid="276"><div class="waypoint" data-reactid="277"></div><a class="section-anchor" data-reactid="278"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="279"><div class="container" data-reactid="280"><div class="columns sixteen" data-reactid="281"><div class="s-title-group " data-reactid="282"><div data-reactid="283"><div class="s-subtitle" data-reactid="284"><div class="s-component s-text" data-reactid="285"><h4 class="s-component-content s-font-heading" data-reactid="286"><p>1.1 Project Description</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="287"><div class="s-repeatable s-block s-component s-mh " data-reactid="288"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="289"><span data-reactid="290"><div data-reactid="291"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="292"><div class=" columns sixteen" data-reactid="293"><div class="s-component s-text" data-reactid="294"><div class="s-component-content s-font-body" data-reactid="295"><p style="text-align: left;">As the first high school team of BGI-college, we endeavour to construct a paper-based cell-free system with whose function is to measure the concentration of target molecules in the sample within a certain range. What matters the most is that this attempt can be attributed into the application of bionics as the transcription and translation devices of the original cell is still present in the cell smear and thus a testing platform for a variety of molecules is created, and hereby being relieved of comprehensive limitations of using living cells for testing. This test paper integrated with biosensors for target molecules enabled the testing of the concentration of a variety of target molecules(various hormones, glucose, fatty acid or nitrates,etc.) in various clinical samples of plasma, urine or saliva. Quantitative results can be shown by the output of a variety of proteins.(light emission of GFP or the changing of colour initiated by LacZ,etc.). The high portability of the test paper and the small duration of the testing process of clinical samples, should the test paper be successfully manufactured, shall relieve millions of patients of comprehensive time-consuming and perhaps agonising medical inspections—these could be carried out at home! The test paper system, presumably, transcends mainstream medical inspections of clinical samples in means of efficiency and convenience.</p><p style="text-align: left;"> </p><p style="text-align: left;">Our idea originated from comprehensive discussions that eliminated the alternatives of acetochlor test papers which already exists in the market at a feasible price and the cholinesterase injection plan for protection of nerve system against Sarin gas as the project relied upon the injected medicine’s access through the Brain-Blood Barriers——an unsolvable dilemma. The idea of cell-free test paper system came up before we came across two articles that outlined the feasibility of a paper-based cell-free system for inspection of clinical samples targeting at specific molecules(1)(2). With the solid theoretical foundation and overwhelming feasibility compared to the other ideas listed above, the cell-free test paper system was chosen by the team unanimously. In late April, the designing of our plasmids was initiated via comprehensive methods, which, upon finishing even as we post this passage, has gone through three phases. Upon the biosensor’s contact with the target molecule, the genetic circuit will produce an output with the fluorescence of a GFP.</p><p style="text-align: left;"> </p><p style="text-align: left;">Our goals go as follows. The very foundation of our targets is to apply our cell-free system, the system of cell environment simulation which consists of cell-extract and substrate that enables DNA expression in vitro to a mature test paper module. Laboratory sample and clinical sample tests will be carried out to assess the effectiveness of the coalition. To make that happen, we will attempt on testing the feasibility of our test paper prototype in pursuit of building up a semi-quantitative test-paper system. Constant amendments, adjustments and improvements to the laboratory tasks specified in the original plans and/or to the plans themselves may be made for the better in accordance with the outcome of the attempt of putting theoretical research and arrangements into practice.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="296"><span data-reactid="297"><div data-reactid="298"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="299"><div class=" columns sixteen" data-reactid="300"><div class="s-component s-text" data-reactid="301"><div class="s-component-content s-font-body" data-reactid="302"><p style="font-size: 80%;"> </p><p style="text-align: left; font-size: 80%;"><em>1. A Courbet, D Endy, E Renard, F Molina, Jérôme Bonnet, Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Science Translational Medicine, Vol. 7, Issue 289, pp. 289ra83 (2015)</em></p><p style="text-align: left; font-size: 80%;"><em>2. Keith Pardee, Alexander A. Green, Tom Ferrante, D. Ewen Cameron, Ajay DaleyKeyser, Peng Yin, James J. Collins, Paper-Based Synthetic Gene Networks. Cell 159, 940–954 (2014)</em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="303"><span data-reactid="304"><div data-reactid="305"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="306"><div class=" columns sixteen" data-reactid="307"><div class="s-separator" data-reactid="308"><div class="s-separator-content" data-reactid="309"><div class="separator" data-reactid="310"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_e2881ddf-6687-4635-a688-c18104aad896" data-reactid="311"><div class="waypoint" data-reactid="312"></div><a class="section-anchor" data-reactid="313"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="314"><div class="container" data-reactid="315"><div class="columns sixteen" data-reactid="316"><div class="s-title-group " data-reactid="317"><div data-reactid="318"><div class="s-subtitle" data-reactid="319"><div class="s-component s-text" data-reactid="320"><h4 class="s-component-content s-font-heading" data-reactid="321"><p>1.2 Target</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="322"><div class="s-repeatable s-block s-component s-mh " data-reactid="323"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="324"><span data-reactid="325"><div data-reactid="326"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="327"><div class=" columns sixteen" data-reactid="328"><div class="s-component s-text" data-reactid="329"><div class="s-component-content s-font-body" data-reactid="330"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="331"><span data-reactid="332"><div data-reactid="333"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="334"><div class=" columns sixteen" data-reactid="335"><div class="s-separator" data-reactid="336"><div class="s-separator-content" data-reactid="337"><div class="separator" data-reactid="338"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_02f15f0e-2cff-4925-bb68-39aa47705391" data-reactid="339"><div class="waypoint" data-reactid="340"></div><a class="section-anchor" data-reactid="341"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="342"><div class="container" data-reactid="343"><div class="columns sixteen" data-reactid="344"><div class="s-title-group " data-reactid="345"><div data-reactid="346"><div class="s-subtitle" data-reactid="347"><div class="s-component s-text" data-reactid="348"><h4 class="s-component-content s-font-heading" data-reactid="349"><p>1.3 Detailed description</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="350"><div class="s-repeatable s-block s-component s-mh " data-reactid="351"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="352"><span data-reactid="353"><div data-reactid="354"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="355"><div class=" columns sixteen" data-reactid="356"><div class="s-component s-text" data-reactid="357"><div class="s-component-content s-font-body" data-reactid="358"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="359"><span data-reactid="360"><div data-reactid="361"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="362"><div class=" columns sixteen" data-reactid="363"><div class="s-component s-image" data-reactid="364"><span data-reactid="365"></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="366"><span data-reactid="367"><div data-reactid="368"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="369"><div class=" columns sixteen" data-reactid="370"><div class="s-component s-text" data-reactid="371"><div class="s-component-content s-font-body" data-reactid="372"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="373"><span data-reactid="374"><div data-reactid="375"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="376"><div class=" columns sixteen" data-reactid="377"><div class="s-component s-image" data-reactid="378"><span data-reactid="379"></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="380"><span data-reactid="381"><div data-reactid="382"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="383"><div class=" columns sixteen" data-reactid="384"><div class="s-component s-text" data-reactid="385"><div class="s-component-content s-font-body" data-reactid="386"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="387"><span data-reactid="388"><div data-reactid="389"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="390"><div class=" columns sixteen" data-reactid="391"><div class="s-separator" data-reactid="392"><div class="s-separator-content" data-reactid="393"><div class="separator" data-reactid="394"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_62682e60-a935-4c0a-8574-655d9d852ef9" data-reactid="395"><div class="waypoint" data-reactid="396"></div><a class="section-anchor" data-reactid="397"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="398"><div class="container" data-reactid="399"><div class="columns sixteen" data-reactid="400"><div class="s-title-group " data-reactid="401"><div data-reactid="402"><div class="s-subtitle" data-reactid="403"><div class="s-component s-text" data-reactid="404"><h4 class="s-component-content s-font-heading" data-reactid="405"><p>1.4 Our results </p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="406"><div class="s-repeatable s-block s-component s-mh " data-reactid="407"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="408"><span data-reactid="409"><div data-reactid="410"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="411"><div class=" columns sixteen" data-reactid="412"><div class="s-component s-text" data-reactid="413"><div class="s-component-content s-font-body" data-reactid="414"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="415"><span data-reactid="416"><div data-reactid="417"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="418"><div class=" columns sixteen" data-reactid="419"><div class="s-separator" data-reactid="420"><div class="s-separator-content" data-reactid="421"><div class="separator" data-reactid="422"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_ffdeee76-9bf3-483a-b433-e1fb24f28975" data-reactid="423"><div class="waypoint" data-reactid="424"></div><a class="section-anchor" data-reactid="425"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="426"><div class="container" data-reactid="427"><div class="columns sixteen" data-reactid="428"><div class="s-title-group " data-reactid="429"><div data-reactid="430"><div class="s-subtitle" data-reactid="431"><div class="s-component s-text" data-reactid="432"><h4 class="s-component-content s-font-heading" data-reactid="433"><p>1.5 Lab summary </p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="434"><div class="s-repeatable s-block s-component s-mh " data-reactid="435"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="436"><span data-reactid="437"><div data-reactid="438"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="439"><div class=" columns sixteen" data-reactid="440"><div class="s-component s-text" data-reactid="441"><div class="s-component-content s-font-body" data-reactid="442"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="443"><span data-reactid="444"><div data-reactid="445"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="446"><div class=" columns sixteen" data-reactid="447"><div class="s-separator" data-reactid="448"><div class="s-separator-content" data-reactid="449"><div class="separator" data-reactid="450"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_164e0d20-8b85-477f-9ec7-8159128c8a90" data-reactid="451"><div class="waypoint" data-reactid="452"></div><a class="section-anchor" data-reactid="453"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="454"><div class="container" data-reactid="455"><div class="columns sixteen" data-reactid="456"><div class="s-title-group " data-reactid="457"><div data-reactid="458"><div class="s-subtitle" data-reactid="459"><div class="s-component s-text" data-reactid="460"><h4 class="s-component-content s-font-heading" data-reactid="461"><p>1.6 Lab Journal </p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="462"><div class="s-repeatable s-block s-component s-mh " data-reactid="463"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="464"><span data-reactid="465"><div data-reactid="466"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="467"><div class=" columns sixteen" data-reactid="468"><div class="s-component s-text" data-reactid="469"><div class="s-component-content s-font-body" data-reactid="470"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="471"><span data-reactid="472"><div data-reactid="473"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="474"><div class=" columns sixteen" data-reactid="475"><div class="s-separator" data-reactid="476"><div class="s-separator-content" data-reactid="477"><div class="separator" data-reactid="478"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_7681efda-5f52-46c5-9143-9ff61605321f" data-reactid="479"><div class="waypoint" data-reactid="480"></div><a class="section-anchor" data-reactid="481"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="482"><div class="container" data-reactid="483"><div class="columns sixteen" data-reactid="484"><div class="s-title-group " data-reactid="485"><div data-reactid="486"><div class="s-subtitle" data-reactid="487"><div class="s-component s-text" data-reactid="488"><h4 class="s-component-content s-font-heading" data-reactid="489"><p>1.7 Intellectual Property </p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="490"><div class="s-repeatable s-block s-component s-mh " data-reactid="491"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="492"><span data-reactid="493"><div data-reactid="494"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="495"><div class=" columns sixteen" data-reactid="496"><div class="s-component s-text" data-reactid="497"><div class="s-component-content s-font-body" data-reactid="498"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="499"><span data-reactid="500"><div data-reactid="501"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="502"><div class=" columns sixteen" data-reactid="503"><div class="s-separator" data-reactid="504"><div class="s-separator-content" data-reactid="505"><div class="separator" data-reactid="506"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_2b5c6fea-d812-496c-9508-e8f21c7ff016" data-reactid="507"><div class="waypoint" data-reactid="508"></div><a class="section-anchor" data-reactid="509"></a><div class="lazyload s-no-bg s-block-section s-section" data-reactid="510"><div class="container" data-reactid="511"><div class="columns sixteen" data-reactid="512"><div class="s-title-group " data-reactid="513"><div data-reactid="514"><div class="s-title " data-reactid="515"><div class="s-component s-text" data-reactid="516"><h2 class="s-component-content s-font-title" data-reactid="517"><p>2. Team</p></h2></div></div><div class="s-subtitle" data-reactid="518"><div class="s-component s-text" data-reactid="519"><h4 class="s-component-content s-font-heading" data-reactid="520"><p style="font-size:80%"><a href="#2-1-meet-the-team">2.1 Meet the team</a></p><p style="font-size:80%"><a href="#2-2-team-member-intro">2.2 Team member intro </a></p><p style="font-size:80%"><a href="#2-3-group-pic">2.3 Group pic </a></p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="521"><div class="s-repeatable s-block s-component s-mh " data-reactid="522"></div></div></div></div></li><li class="slide" id="section-f_724ce88b-8a10-41dd-86ea-178e504ca3a4" data-reactid="523"><div class="waypoint" data-reactid="524"></div><a class="section-anchor" data-reactid="525"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="526"><div class="container" data-reactid="527"><div class="columns sixteen" data-reactid="528"><div class="s-title-group " data-reactid="529"><div data-reactid="530"><div class="s-subtitle" data-reactid="531"><div class="s-component s-text" data-reactid="532"><h4 class="s-component-content s-font-heading" data-reactid="533"><p>2.1 Meet the team</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="534"><div class="s-repeatable s-block s-component s-mh " data-reactid="535"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="536"><span data-reactid="537"><div data-reactid="538"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="539"><div class=" columns sixteen" data-reactid="540"><div class="s-component s-text" data-reactid="541"><div class="s-component-content s-font-body" data-reactid="542"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="543"><span data-reactid="544"><div data-reactid="545"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="546"><div class=" columns sixteen" data-reactid="547"><div class="s-separator" data-reactid="548"><div class="s-separator-content" data-reactid="549"><div class="separator" data-reactid="550"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_9bc62bfe-fe53-4694-aa06-c1293b1c70f8" data-reactid="551"><div class="waypoint" data-reactid="552"></div><a class="section-anchor" data-reactid="553"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="554"><div class="container" data-reactid="555"><div class="columns sixteen" data-reactid="556"><div class="s-title-group " data-reactid="557"><div data-reactid="558"><div class="s-subtitle" data-reactid="559"><div class="s-component s-text" data-reactid="560"><h4 class="s-component-content s-font-heading" data-reactid="561"><p>2.2 Team member intro </p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="562"><div class="s-repeatable s-block s-component s-mh " data-reactid="563"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="564"><span data-reactid="565"><div data-reactid="566"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="567"><div class=" columns sixteen" data-reactid="568"><div class="s-component s-text" data-reactid="569"><div class="s-component-content s-font-body" data-reactid="570"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="571"><span data-reactid="572"><div data-reactid="573"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="574"><div class=" columns sixteen" data-reactid="575"><div class="s-separator" data-reactid="576"><div class="s-separator-content" data-reactid="577"><div class="separator" data-reactid="578"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_4c421b73-e237-4779-8ee5-04709d302be9" data-reactid="579"><div class="waypoint" data-reactid="580"></div><a class="section-anchor" data-reactid="581"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="582"><div class="container" data-reactid="583"><div class="columns sixteen" data-reactid="584"><div class="s-title-group " data-reactid="585"><div data-reactid="586"><div class="s-subtitle" data-reactid="587"><div class="s-component s-text" data-reactid="588"><h4 class="s-component-content s-font-heading" data-reactid="589"><p>2.3 Group pic</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="590"><div class="s-repeatable s-block s-component s-mh " data-reactid="591"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="592"><span data-reactid="593"><div data-reactid="594"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="595"><div class=" columns sixteen" data-reactid="596"><div class="s-component s-text" data-reactid="597"><div class="s-component-content s-font-body" data-reactid="598"><p>(Blank)</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="599"><span data-reactid="600"><div data-reactid="601"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="602"><div class=" columns sixteen" data-reactid="603"><div class="s-separator" data-reactid="604"><div class="s-separator-content" data-reactid="605"><div class="separator" data-reactid="606"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_4e622fe2-969f-4d8f-a449-ebca10a8d7be" data-reactid="607"><div class="waypoint" data-reactid="608"></div><a class="section-anchor" data-reactid="609"></a><div class="lazyload s-no-bg s-block-section s-section" data-reactid="610"><div class="container" data-reactid="611"><div class="columns sixteen" data-reactid="612"><div class="s-title-group " data-reactid="613"><div data-reactid="614"><div class="s-title " data-reactid="615"><div class="s-component s-text" data-reactid="616"><h2 class="s-component-content s-font-title" data-reactid="617"><p>3. Human practice</p></h2></div></div><div class="s-subtitle" data-reactid="618"><div class="s-component s-text" data-reactid="619"><h4 class="s-component-content s-font-heading" data-reactid="620"><p>3.1Sponsorship&amp; Attribution</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="621"><div class="s-repeatable s-block s-component s-mh " data-reactid="622"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="623"><span data-reactid="624"><div data-reactid="625"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="626"><div class=" columns sixteen" data-reactid="627"><div class="s-component s-text" data-reactid="628"><div class="s-component-content s-font-body" data-reactid="629"><p>(Blank)</p></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_f8941d76-dd7b-46bd-bff8-eb3625a0ec1c" data-reactid="630"><div class="waypoint" data-reactid="631"></div><a class="section-anchor" data-reactid="632"></a><div class="lazyload s-no-bg s-block-section s-section" data-reactid="633"><div class="container" data-reactid="634"><div class="columns sixteen" data-reactid="635"><div class="s-title-group " data-reactid="636"><div data-reactid="637"><div class="s-title " data-reactid="638"><div class="s-component s-text" data-reactid="639"><h2 class="s-component-content s-font-title" data-reactid="640"><p>4. Safety</p></h2></div></div><div class="s-subtitle" data-reactid="641"><div class="s-component s-text" data-reactid="642"><h4 class="s-component-content s-font-heading" data-reactid="643"><p style="font-size:80%"><a href="#">4.1 Laboratory safety assessment</a></p><p style="font-size:80%">4.1.2 Materials used</p><p style="font-size:80%">4.1.3 Protection</p><p style="font-size:80%">4.1.4 Disposal</p><p style="font-size:80%"><a href="#">4.2 Identiﬁed possible risks of our project</a></p><p style="font-size:80%">4.2.1 Risks to the safety and health of team members, or other people working in the lab</p><p style="font-size:80%">4.2.2 Risks to the safety and health of the general public (if any biological materials escaped from our lab)</p><p style="font-size:80%">4.2.3 Risks to the environment (from waste disposal, or from materials escaping from our lab)<br>4.2.4 Risks to security through malicious misuse by individuals, groups, or countries</p><p style="font-size:80%">4.2.5 What new risks might arise from our project's growth?</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="644"><div class="s-repeatable s-block s-component s-mh " data-reactid="645"></div></div></div></div></li><li class="slide" id="section-f_aa116216-5f93-4a06-8510-a3a8d66910b8" data-reactid="646"><div class="waypoint" data-reactid="647"></div><a class="section-anchor" data-reactid="648"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="649"><div class="container" data-reactid="650"><div class="columns sixteen" data-reactid="651"><div class="s-title-group " data-reactid="652"><div data-reactid="653"><div class="s-subtitle" data-reactid="654"><div class="s-component s-text" data-reactid="655"><h4 class="s-component-content s-font-heading" data-reactid="656"><p>4.1 Laboratory safety assessment</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="657"><div class="s-repeatable s-block s-component s-mh " data-reactid="658"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="659"><span data-reactid="660"><div data-reactid="661"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="662"><div class=" columns sixteen" data-reactid="663"><div class="s-component s-text" data-reactid="664"><div class="s-component-content s-font-body" data-reactid="665"><p>4.1.1 Materials used</p><p> </p><p>(Blank)</p><p> </p><p>4.1.2 Protection</p><p> </p><p>(Blank)</p><p> </p><p>4.1.3 Disposal</p><p> </p><p>(Blank)</p><p> </p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="666"><span data-reactid="667"><div data-reactid="668"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="669"><div class=" columns sixteen" data-reactid="670"><div class="s-separator" data-reactid="671"><div class="s-separator-content" data-reactid="672"><div class="separator" data-reactid="673"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_dd9ebd6d-a000-472b-bc80-6d88b2c1b02e" data-reactid="674"><div class="waypoint" data-reactid="675"></div><a class="section-anchor" data-reactid="676"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/33.jpg" data-reactid="677"><div class="container" data-reactid="678"><div class="columns sixteen" data-reactid="679"><div class="s-title-group " data-reactid="680"><div data-reactid="681"><div class="s-subtitle" data-reactid="682"><div class="s-component s-text" data-reactid="683"><h4 class="s-component-content s-font-heading" data-reactid="684"><p>4.2 Identiﬁed possible risks of our project</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="685"><div class="s-repeatable s-block s-component s-mh " data-reactid="686"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="687"><span data-reactid="688"><div data-reactid="689"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="690"><div class=" columns sixteen" data-reactid="691"><div class="s-component s-text" data-reactid="692"><div class="s-component-content s-font-body" data-reactid="693"><p>4.2.1 Risks to the safety and health of team members, or other people working in the lab</p><p> </p><p>(Blank)</p><p> </p><p>4.2.2 Risks to the safety and health of the general public (if any biological materials escaped from our lab) </p><p> </p><p>(Blank)</p><p> </p><p>4.2.3 Risks to the environment (from waste disposal, or from materials escaping from our lab)</p><p> </p><p>(Blank)</p><p><br>4.2.4 Risks to security through malicious misuse by individuals, groups, or countries</p><p> </p><p>(Blank)</p><p> </p><p>4.2.5 What new risks might arise from our project's growth?</p><p> </p><p>(Blank)</p><p> </p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="694"><span data-reactid="695"><div data-reactid="696"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="697"><div class=" columns sixteen" data-reactid="698"><div class="s-separator" data-reactid="699"><div class="s-separator-content" data-reactid="700"><div class="separator" data-reactid="701"></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide" id="section-f_d39f9726-5fc7-4410-b0b0-401e5384d250" data-reactid="702"><div class="waypoint" data-reactid="703"></div><a class="section-anchor" data-reactid="704"></a><div class="lazyload s-no-bg s-block-section s-section" data-reactid="705"><div class="container" data-reactid="706"><div class="columns sixteen" data-reactid="707"><div class="s-title-group " data-reactid="708"><div data-reactid="709"><div class="s-title " data-reactid="710"><div class="s-component s-text" data-reactid="711"><h2 class="s-component-content s-font-title" data-reactid="712"><p>5. Blog</p></h2></div></div><div class="s-subtitle" data-reactid="713"><div class="s-component s-text" data-reactid="714"><h4 class="s-component-content s-font-heading" data-reactid="715"><p>Feel free to subscribe our blog!</p></h4></div></div></div></div></div><div class="alignment-container " data-reactid="716"><div class="s-repeatable s-block s-component s-mh " data-reactid="717"></div></div></div></div></li><li class="slide" id="section-f_152a36d5-4f8a-493b-b1a2-1ed21c61d6b3" data-reactid="718"><div class="waypoint" data-reactid="719"></div><a class="section-anchor" data-reactid="720"></a><div class="lazyload s-no-bg s-blog-section s-section" data-reactid="721"><div class="waypoint" data-reactid="722"></div><div class="container" data-reactid="723"><div data-reactid="724"><!-- react-empty: 725 --><div class="s-component s-async-wrapper" data-reactid="726"><div class="s-loading" data-reactid="727"></div><div class="waypoint" data-reactid="728"></div></div></div></div></div><div class="waypoint" data-reactid="729"></div></li></ul><!-- react-empty: 730 --><div id="strikingly-tooltip-container" data-reactid="731"> </div><div class="s-footer-section s-section " data-reactid="732"><div class="container s-layout-vertical" data-reactid="733"><div class="columns row sixteen" data-reactid="734"><div class="" data-reactid="735"><div class="s-component s-async-wrapper" data-reactid="736"><div class="s-loading" data-reactid="737"></div><div class="waypoint" data-reactid="738"></div></div></div><div class="s-footer-text" data-reactid="739"><div class="s-component s-text" data-reactid="740"><div class="s-component-content s-font-body" data-reactid="741"><p>iGEM BGI College© 2016</p></div></div></div></div></div></div><!-- react-empty: 742 --><div class="s-floated-components" data-reactid="743"><div class="ecommerce-buy-dialog s-edit-modal extend" id="ecommerce-buy-dialog" data-reactid="744"><div class="s-async-wrapper s-component" data-reactid="745"><div class="s-loading" data-reactid="746"></div></div></div><div id="s-ecommerce-shopping-cart-wrapper" class="s-ecommerce-shopping-cart-wrapper" data-reactid="747"></div></div></div></div> <div id='fb-root'></div> <div id='app-script-root'></div> <div id='app-view-root'></div> <script id='blog-collection-tmpl' type='text/html'> <div class='s-blog-col-head'></div> <div class='s-blog-col-body'> {{ _.each(blogPosts, function(blog) { }} <div class='s-blog-entry'> <div class='s-blog-entry-inner'> {{ if ($S && $S.conf && $S.conf.preview_mode) { }} <a class='s-blog-overlay-link' href='{{=blog.publicUrl}}'>&nbsp;</a> {{ } else { }} <a class='s-blog-overlay-link' href='{{=blog.relativeUrl}}'>&nbsp;</a> {{ } }} {{ if (blog.iconUrl) { }} <div class='s-blog-entry-left'> {{ if (UrlHelper.imageHasContent(blog.iconUrl)) { }} <div class='s-blog-avatar' style='background-image:url({{=blog.iconUrl}});'></div> {{ } else { }} <div class='s-blog-default-avatar'> <div class='entypo-bookmark'></div> </div> {{ } }} </div> {{ } }} <div class='s-blog-entry-right'> <div class='s-blog-details'> <div class='s-blog-details-head s-blog-row'> <div class='s-blog-title s-font-heading'> {{=blog.title}} </div> <div class='s-blog-date'> {{=blog.publishedAt}} </div> </div> {{ if (blogSettings.previewLayout == 1) { }} <div class='s-blog-details-blurb s-blog-row'> {{=blog.blurb}} </div> {{ } }} </div> </div> </div> </div> {{ }) }} {{ if (blogPosts.length == 0) { }} <div class='s-blog-entry'> <div class='s-common-status no-posts-error'> There are no blog posts. </div> </div> {{ } }} </div> {{ if (blogPosts.length > 0) { }} <div class='s-blog-col-foot'> <a class='s-blog-link s-blog-next-link' href='javascript:void(0);'> &laquo; Newer </a> &nbsp; <ul class='s-blog-pagination' style='display:none;'> <li> <a class='s-blog-link s-blog-pagination-page' href='javascript:void(0);'></a> </li> </ul> &nbsp; <a class='s-blog-link s-blog-prev-link' href='javascript:void(0);'> Older &raquo; </a> </div> {{ } }} </script> <script src="//ajax.googleapis.com/ajax/libs/jquery/1.10.0/jquery.min.js"></script> <script>
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Difference between revisions of "Team:BGIC China"

Revision as of 07:22, 2 July 2016

BGI-Colleg​e China

Project

Open source Cell-free system based test paper platform

Team

About our team

Human Practice

Safety

Blog

1. Project

1.1 Project Description

1.2 Target

1.3 Detailed description

1.4 Our results

1.5 Lab summary

1.6 Lab Journal

1.7 Intellectual Property

2. Team

2.1 Meet the team2.2 Team member intro 2.3 Group pic

2.1 Meet the team

2.2 Team member intro

2.3 Group pic

3. Human practice

3.1Sponsorship& Attribution

4. Safety

4.1 Laboratory safety assessment

4.2 Identiﬁed possible risks of our project

5. Blog

Feel free to subscribe our blog!

BGI-College China

2.1 Meet the team
2.2 Team member intro
2.3 Group pic