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| | <div id="form" style="background: rgba(100%,100%,100%,0); overflow-x:hidden;overflow-y:hidden; "> | | <div id="form" style="background: rgba(100%,100%,100%,0); overflow-x:hidden;overflow-y:hidden; "> |
| − | <table width="800" align="center" style="background-color: rgba(100%,100%,100%,0)"> | + | <table width="800" align="center" style="background-color: rgba(100%,100%,100%,0)"> |
| | <td colspan=2 align=center> | | <td colspan=2 align=center> |
| − | <img src="https://static.igem.org/mediawiki/2015/7/7e/2015tcutaiwanSafety.jpg" width=750 height=300 border="0" id="Image1" /> | + | <img src="https://static.igem.org/mediawiki/2016/5/55/Tcu_sub11.jpg" width=750 height=400 border="0" id="Image1" /> |
| | </td> | | </td> |
| | </table> | | </table> |
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| − | <table width="100%" align="center"> | + | <table> |
| − | <tr>
| + | <td align=center colspan=2><font size=25>Biosafety</font></td> |
| − | <td align="center" width="45%"><span style="font-family:Arial Black;color:#6B8E23"><font size="6"><font-weight: 800;>1.About Our
| + | |
| − | Lab</br></font></span></td>
| + | |
| − | <td align="center" width="65%"><img src="https://static.igem.org/mediawiki/2015/4/49/TCU_Taiwanlabn.jpg" align=center width="60%" align="center" title="About Our
| + | |
| − | Lab" </td></br>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <table width="100%" align="center">
| + | |
| − | <tr>
| + | |
| − | <td width="3%"></td>
| + | |
| − | <td >
| + | |
| − | <span style="font-family:Arial Black;"><font size="5">What is the Safety Level of your lab?</font>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| | <tr> | | <tr> |
| − | <td width="3%"></td> | + | <th align=left colspan=2><span style="font-family:Calibri;text-align:justify;"><font size="5"> |
| − | <td >
| + | A good product is defined as a product that benefits the people and do not harm the surrounding environment. TCU is always stressing on biosafety among its curriculums. Hence, we take this biosafety issue very seriously.<br><br> |
| − | <span style="font-family:Calibri;"><font size="5">We have several different lab spaces with different Safety Levels</br>(We do our experiment of <I>Staphylococcus aureus</I> in a level 2 (moderate risk) lab and use <I>E.coli</I> in a level 1 (low risk) lab.)</br></font> | + | There are some biosafety issues that were raise by other teams and here is what we have found after our research.<br><br> |
| − | </td> | + | 1.Is <I>E. coli BL21</I> safe for our environment?<br><br> |
| − | </tr>
| + | Chromo Diabetector is composed by <I>BL21</I>. Base on the website, it says that<I> BL21</I> is safe for the environment. |
| − | </table> | + | </font></span></td> |
| − | <table width="100%" align="center"> | + | |
| − | <tr>
| + | |
| − | <td width="3%"></td>
| + | |
| − | <td >
| + | |
| − | <span style="font-family:Arial Black;"><font size="5">Which work areas do you use to handle biological materials?</font>
| + | |
| − | </td> | + | |
| − | </tr>
| + | |
| | <tr> | | <tr> |
| − | <td width="3%"></td> | + | <td colspan=2> |
| − | <td >
| + | <a href="https://www.neb.com/~/media/Catalog/All-Products/0B28021B9A36470BB46B318DAD19ED4F/MSDS/sdsC2527gh.pdf ">Resources:https://www.neb.com/~/media/Catalog/All-Products/0B28021B9A36470BB46B318DAD19ED4F/MSDS/sdsC2527gh.pdf</a><br><br> |
| − | <span style="font-family:Calibri;"><font size="5">1. Open bench</br>2. Biosafety cabinet / laminar flow hood</br>3.Chemical fume hood</br></font>
| + | |
| | </td> | | </td> |
| − | </tr>
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| − | </table>
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| − |
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| − | <table width="100%" align="center">
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| − | <tr>
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| − | <td width="3%"></td>
| |
| − | <td >
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| − | <span style="font-family:Arial Black;"><font size="5">Who is responsible for the safety of biology labs at your institution? What are the guidelines for laboratory biosafety?</font>
| |
| − | </td><td width="3%"></td>
| |
| − | </tr>
| |
| | <tr> | | <tr> |
| − | <td width="3%"></td> | + | <th align=left colspan=2><span style="font-family:Calibri;text-align:justify;"><font size="5"> |
| − | <td >
| + | 2. Is dh5α is safe for our environment?<br><br> |
| − | <span style="font-family:Calibri;"><font size="5">Our biosafety level 1 of biology labs is responsible by our instructor, Shun-Ping Huang. Our biosafety level 2 of biology labs is responsible by our instructor, Kai Chih Chang. Our guidelines is follow the law in our country, and you can see about that in the next question.</br></font> | + | Based on the website, it says that dh5α does not harm a healthy adult human.<br><br> |
| − | </td><td width="3%"></td>
| + | </font></span></td> |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <table width="100%" align="center">
| + | |
| − | <tr>
| + | |
| − | <td width="3%"></td>
| + | |
| − | <td align="left"><span style="font-family:Arial Black;color:#6B8E23"><font size="6"><font-weight: 800;>2.Local Rules and Regulations</font></span></td>
| + | |
| − | </br>
| + | |
| − | </tr>
| + | |
| − | </table> | + | |
| − | <table width="100%" align="center">
| + | |
| − | <tr>
| + | |
| − | <td width="3%"></td>
| + | |
| − | <td >
| + | |
| − | <span style="font-family:Arial Black;"><font size="5">In your country /region, what are the laws and regulations that govern biosafety in research laboratories?</font>
| + | |
| − | </td><td width="3%"></td> | + | |
| − | </tr>
| + | |
| | <tr> | | <tr> |
| − | | + | <td colspan=2> |
| − | <td width="3%"></td><td>
| + | <a href="https://www.neb.com/~/media/Catalog/All-Products/0B28021B9A36470BB46B318DAD19ED4F/MSDS/sdsC2527gh.pdf ">Resources:https://www.neb.com/~/media/Catalog/All-Products/0B28021B9A36470BB46B318DAD19ED4F/MSDS/sdsC2527gh.pdf</a> |
| − | <span style="font-family:Calibri;"><font size="5">Our country has the law that govern biosafety in research laboratories. This law is from Centers for Disease Control, Taiwan, and it has a lot of sections, laboratories safety rules, biosafety rules, safety of infectious biological material, protective equipment rules and the rules of biotoxin. We have already detailed read these rules, and we will do our experiment according them.
| + | </td> |
| − | <a href="http://www.cdc.gov.tw/professional/info.aspx?treeid=beac9c103df952c4&nowtreeid=d541f7cbe586397a&tid=FE748BF304AB8DB4">Centers for Disease Control,Taiwan</a></br></font> | + | |
| − | </td><td width="3%"></td>
| + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <table width="100%" align="center">
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| − | <tr>
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| − | <td width="3%"></td>
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| − | <td align="left"><span style="font-family:Arial Black;color:#6B8E23"><font size="6"><font-weight: 800;>3.Safety Training</font></span></td>
| + | |
| − | </br> | + | |
| − | </tr>
| + | |
| − | </table>
| + | |
| − | <table width="100%">
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| | <tr> | | <tr> |
| − | <td width="3%"></td> | + | <th align=left colspan=2><span style="font-family:Calibri;text-align:justify;"><font size="5"> |
| − | <td width="90%">
| + | In our lab, every waste material will be stored properly inside the wasted material trash. These materials will be handed off to the ministry of environment to be disposed properly so that it does not cause any chemical pollution towards the environment. |
| − | <span style="font-family:Calibri;"><font size="5">We have already received safety training.<br> | + | </font></span></td> |
| − | 1) Universal lab safety rules: wearing lab coat, glasses and rubber gloves, washing hands, keeping work area uncluttered, etc.</br>
| + | |
| − | 2) Know the location of fire extinguisher, eyewash station, chemical spill kits and else <br>
| + | |
| − | 3) First aid lesson<br>
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| − | 4) Help yourself: memorizing emergency safety telephone number.<br>
| + | |
| − | 5) Use laminar flow to do the experiment of biosafety Level 2.<br></font></span>
| + | |
| − | </td><td width="3%"></td> | + | |
| − | </tr>
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| − | <br>
| + | |
| − | </table>
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| | <tr> | | <tr> |
| − | | + | <th width=50% align=center> |
| − | <table width="100%"><span style="font-family:Arial Black;color:#6B8E23"><font size="6"><font-weight: 800;> | + | <img src="https://static.igem.org/mediawiki/2016/9/97/Tcu_sm16.jpg" width=350 height=300 border="0" id="Image1" /> |
| − | <tr>
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| − | <td width="3%"></td>
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| − | <td width="20%" align="center">
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| − | <img src="https://static.igem.org/mediawiki/2015/a/a3/TCU_TAIWAN_SAFETY_Q3-1.jpg"> | + | |
| | </td> | | </td> |
| − | | + | <th width=50% align=center> |
| − | <td width="40%" align="center"> | + | <img src="https://static.igem.org/mediawiki/2016/4/44/Tcu_sm17.jpg" width=350 height=300 border="0" id="Image1" /> |
| − | | + | |
| − | <img src="https://static.igem.org/mediawiki/2015/4/44/TCU_TAIWAN_SAFETY_Q3-2.jpg" width=250> | + | |
| | </td> | | </td> |
| − | <td width="40%" align="center">
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| − | <img src="https://static.igem.org/mediawiki/2015/9/9e/TCU_TAIWAN_SAFETY_Q3-3.jpg" width=250>
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| − | </td>
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| − | </tr>
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| − | <td width="5%"></td>
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| − | <td width="20%" align="left">
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| − | This is escape routes of our laboratory building.
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| − | </td>
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| − | <td width="40%" align="left">
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| − | This is Emergency notification flowchart of our biosafety level 1 lab.
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| − | </td>
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| − | <td width="40%" align="left">
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| − | This is Emergency notification flowchart of our biosafety level 2 lab.
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| − | </td>
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| − | </tr>
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| − |
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| − | </font>
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| − | </span>
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| | </table> | | </table> |
| − | <table width="100%" align="center">
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| − | <tr>
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| − | <td width="3%"></td>
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| − | <td align="left"><span style="font-family:Arial Black;color:#6B8E23"><font size="6"><font-weight: 800;>4.The Organisms and Parts</font></span></td>
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| − | </br>
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| − | </tr>
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| − | </table>
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| − | </br></br>
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| − | <table width="90%" border="1" cellpadding="0" cellspacing="0" align="center">
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| − | <tr>
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| − | <td>Species name (including strain)</td>
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| − | <td>Risk Group</td>
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| − | <td>Risk Group Source</td>
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| − | <td>Disease risk to humans?</td>
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| | | | |
| − |
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| − | <td>How did you acquire it?</td>
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| − | <td>How will you use it?</td>
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| − |
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| − | </tr>
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| − | <tr>
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| − | <td><I>E. coli</I> DH5α</td>
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| − | <td>Level 1</td>
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| − | <td>WHO</td>
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| − | <td>do not cause disease in healthy adult humans.</td>
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| − |
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| − |
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| − | <td>bought from yeastern</td>
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| − | <td>Competent cell for our plasmid</td>
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| − |
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| − | </tr>
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| − | <tr>
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| − | <td><I>Staphylococcus aureus</I><br />
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| − | (ATCC number: 25923)</td>
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| − | <td>Level 2</td>
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| − | <td>WHO</td>
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| − | <td>Cause the infection of wound, and make wound healing more difficult</td>
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| − |
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| − | <td>Acquired from another lab in our school</td>
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| − | <td>disinfect test</td>
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| − |
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| − | </tr>
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| − | <tr>
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| − | <td white-space:nowrap;>Squamous epithelial cells<br>(HaCat)</td>
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| − | <td>Level 1</td>
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| − | <td>WHO</td>
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| − | <td>do not cause disease in healthy adult humans.</td>
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| − |
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| − | <td>Acquired from another lab in our school</td>
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| − | <td>wound healing test</td>
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| − |
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| − | </tr>
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| − | <tr>
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| − | <td>Dermal microvascular<br>endothelium cells<br>(ATCC number: CRL-3243)</td>
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| − | <td>Level 1</td>
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| − | <td>WHO</td>
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| − | <td>do not cause disease in healthy adult humans.</td>
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| − |
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| − | <td>Acquired from another lab in our school</td>
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| − | <td>wound healing test</td>
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| − |
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| − | </tr>
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| − | <tr>
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| − | <td>NMRI mice</td>
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| − | <td>Level 1</td>
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| − | <td>WHO</td>
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| − | <td>do not cause disease in healthy adult humans.</td>
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| − |
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| − | <td>Acquired from another lab in our school</td>
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| − | <td>wound healing test</td>
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| − |
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| − | </tr>
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| − | </table>
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| − | <table width="100%" align="center">
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| − | <td width="3%"></td>
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| − | <td align="left"><span style="font-family:Arial Black;color:#6B8E23"><font size="6"><font-weight: 800;>5.Risks of Our
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| − | Project </font></span></td>
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| − | </br>
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| − | </tr>
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| − | </table>
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| − | <td >
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| − | <span style="font-family:Arial Black;"><font size="5">Risks to the safety and health of team members, or other people working in the lab:</br></font></span>
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| − | <span style="font-family:Calibri;text-align:justify;"><font size="5">
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| − | Some reagents we used are poison for human. Such as EtBr, chloroform, isopropanol, etc. To prevent their harm, we wear rubber gloves, lab coat and used chemical hood when using them.</br></br>
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| − | <span style="font-family:Arial Black;"><font size="5">Risks to the safety and health of the general public:</br></font></span>
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| − | Our antimicrobial peptides, Signferin is from <I>Crinia signifera</I>, and Epinecidin-1 is from <I>Epinephelus coioides</I>. We use <I>E.coli</I> to synthesizes the peptide. The antimicrobial peptides will be applied to open wounds or burns, the small peptides might induce allergic reactions in some individuals. To reduce this risk, allergic test can be preformed prior to the application of the deressing.</br></br>
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| − |
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| − | <span style="font-family:Arial Black;"><font size="5">Risks to the environment:</br></font></span>
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| − | We modified <I>E.coli</I> to produce antimicrobial peptides, if these <I>E.coli</I> are released to environment, they might cause genetic pollution. To prevent this problem, we disposal our waste according the rule of our university.</br></br>
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| − |
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| − | </font>
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| − | </td><td width="3%"></td>
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| − | </tr>
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| − | </table>
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| | </body> | | </body> |
| | </html> | | </html> |
| | {{TCU_Taiwan/CSSend}} | | {{TCU_Taiwan/CSSend}} |
