Difference between revisions of "Team:FAFU-CHINA/Basic Part"
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<div> | <div> | ||
| − | + | == Cry4Aa4+Extended FMDV 2A-pChlamy_3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 101: | Line 101: | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cry4Aa4+Extended FMDV 2A-pChlamy == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
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<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cry11Aa4+ Extended FMDV 2A-pChlamy_3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
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<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cyt1-pChlamy_3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 394: | Line 394: | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cyt2-pChlamy_3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
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<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| − | + | == EGFP-pChlamy_3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 596: | Line 596: | ||
</table> | </table> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cry4Aa4+Extended2A+Cyt2-pChlamy3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 698: | Line 698: | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cry4Aa4+Extended2A+Cyt1-pChlamy3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 800: | Line 800: | ||
</table> | </table> | ||
| − | + | == Cry10Aa4+Extended2A+Cyt2-pChlamy3 == | |
<p> </p> | <p> </p> | ||
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 900: | Line 900: | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cry10Aa4+Extended2A+Cyt2-pChlamy3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 998: | Line 998: | ||
</table> | </table> | ||
| − | + | == Cry10Aa4+Extended2A+Cyt1-pChlamy3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 1,100: | Line 1,100: | ||
</table> | </table> | ||
| − | + | == Cry11Aa4+Extended2A+Cyt2-pChlamy3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 1,196: | Line 1,196: | ||
</table> | </table> | ||
| − | + | == Cry11Aa4+Extended2A+Cyt1-pChlamy3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
| Line 1,294: | Line 1,294: | ||
</table> | </table> | ||
<p> </p> | <p> </p> | ||
| − | + | == Cry11Aa4+Extended2A+EGFP-pChlamy3 == | |
<table border="1" cellpadding="0" cellspacing="0"> | <table border="1" cellpadding="0" cellspacing="0"> | ||
Revision as of 11:41, 17 October 2016
Contents
- 1 Basic Part =
- 1.1 Cry4Aa4+Extended FMDV 2A-pChlamy_3
- 1.2 Cry4Aa4+Extended FMDV 2A-pChlamy
- 1.3 Cry11Aa4+ Extended FMDV 2A-pChlamy_3
- 1.4 Cyt1-pChlamy_3
- 1.5 Cyt2-pChlamy_3
- 1.6 EGFP-pChlamy_3
- 1.7 Cry4Aa4+Extended2A+Cyt2-pChlamy3
- 1.8 Cry4Aa4+Extended2A+Cyt1-pChlamy3
- 1.9 Cry10Aa4+Extended2A+Cyt2-pChlamy3
- 1.10 Cry10Aa4+Extended2A+Cyt2-pChlamy3
- 1.11 Cry10Aa4+Extended2A+Cyt1-pChlamy3
- 1.12 Cry11Aa4+Extended2A+Cyt2-pChlamy3
- 1.13 Cry11Aa4+Extended2A+Cyt1-pChlamy3
- 1.14 Cry11Aa4+Extended2A+EGFP-pChlamy3
Basic Part =
Cry4Aa4+Extended FMDV 2A-pChlamy_3
Gene Name |
Cry4Aa4+Extended FMDV 2A |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in DH5α |
Gene Length |
3673bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="263.20001220703125" src="http://b263.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/yfm7rDaljwf3tLDwN85uBcwarSLNpdnfMlUxh1G7pP4!/b/dAcBAAAAAAAA&bo=sAAIAQAAAAADAJw!" width="175.1999969482422">
|
<img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&bo=nQAnAQAAAAADAJ4!" width="156.0">
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA |
Cry4Aa4+Extended FMDV 2A-pChlamy
Gene Name |
Cry10Aa4+Extended FMDV 2A |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in Top10 |
Gene Length |
2158bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">
|
<img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&bo=7QA1AQAAAAADAPw!" width="236.8000030517578">
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA |
Cry11Aa4+ Extended FMDV 2A-pChlamy_3
Gene Name |
Cry11Aa4+ Extended FMDV 2A |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in JM108 |
Gene Length |
2071bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">
|
<img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&bo=qAA1AQAAAAADALk!" width="167.1999969482422"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cyt1-pChlamy_3
Gene Name |
Cyt1 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in JM108 |
Gene Length |
762bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">
|
<img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&bo=sAAYAQAAAAADAIw!" width="175.1999969482422"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cyt2-pChlamy_3
Gene Name |
Cyt1 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in JM108 |
Gene Length |
801bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
|||
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
|||
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
|||
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
|||
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
|||
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
|||
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
|||
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
|||
<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">
|
<img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&bo=4QAZAQAAAAADANw!" width="224.0"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
|
|||
EGFP-pChlamy_3
Gene Name |
06-EGFP（TAA） |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in Top10 |
Gene Length |
732bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"> |
<img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&bo=8AApAQAAAAADAP0!" width="239.1999969482422"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry4Aa4+Extended2A+Cyt2-pChlamy3
Gene Name |
Cry4Aa4+Extended2A+Cyt2 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in Top10 |
Gene Length |
4461bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578">
|
<img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&bo=mADtAAAAAAADAFA!" width="151.1999969482422">
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and BamHI
Lane 2: Plasmid DNA
|
Cry4Aa4+Extended2A+Cyt1-pChlamy3
Gene Name |
Cry4Aa4+Extended2A+Cyt1-pChlamy3 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in JM108 |
Gene Length |
4422bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
|||
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
|||
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
|||
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
|||
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
|||
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
|||
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
|||
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
|||
<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578"> |
<img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&bo=rgAZAQAAAAADAJM!" width="173.60000610351562"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by BamHI and KpnI
Lane 2: Plasmid DNA
|
|
|||
Cry10Aa4+Extended2A+Cyt2-pChlamy3
Gene Name |
Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in DH5α |
Gene Length |
2946bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"> |
<img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&bo=jQAJAQAAAAADAKA!" width="140.0"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt2-pChlamy3
Gene Name |
Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in DH5α |
Gene Length |
2946bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">
|
<img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&bo=kQASAQAAAAADAKc!" width="144.8000030517578"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt1-pChlamy3
Gene Name |
Cry10Aa4+Extended2A+Cyt1-pChlamy3 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in DH5α |
Gene Length |
2907bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
|||
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
|||
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
|||
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
|||
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
|||
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
|||
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
|||
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
|||
<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">
|
<img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&bo=zQAhAQAAAAADAMg!" width="204.8000030517578"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
|
|||
Cry11Aa4+Extended2A+Cyt2-pChlamy3
Gene Name |
Cry11Aa4+Extended2A+Cyt2-pChlamy3 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in Top10 |
Gene Length |
2859bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"> |
<img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&bo=wwADAQAAAAADAOQ!" width="194.39999389648438"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+Cyt1-pChlamy3
Gene Name |
Cry11Aa4+Extended2A+Cyt1-pChlamy3 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in JM108 |
Gene Length |
2820bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"> |
<img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&bo=vQAJAQAAAAADAJA!" width="188.0"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+EGFP-pChlamy3
Gene Name |
Cry11Aa4+Extended2A+EGFP-pChlamy3 |
Cloning Vector |
pChlamy_3 |
Construct Resistant |
Ampicillin in JM108 |
Gene Length |
2790bp |
Cloning Strategy |
XbaI and PstI |
QC Items |
Method |
Specifications |
Results |
Target Sequence |
Sequence alignment |
Sequencing results are consistent to the confirmed sequence. |
Pass |
Vector Sequence |
Sequence alignment |
20bp flanking sequences of the vector are correct. |
Pass |
Reading Frame |
Sequence alignment |
Frame is correct and consistent to the client's requirement. |
Pass |
Fragment Size |
Restriction Digests |
The size of inserted fragment is right and free of any contaminated bands. |
Pass |
Quality/Quantity |
UV spectrophotometry |
Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized |
Pass |
Type of Strain Stock |
Visual inspection |
Bacteria stock. |
Pass |
Appearance |
Visual inspection |
Clear and free of foreign particles, and the strain is clearly visible. |
Pass |
<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"> |
<img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&bo=qQAEAQAAAAADAIk!" width="168.8000030517578"> |
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
