Difference between revisions of "Team:Paris Saclay/Notebook/June/23"
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{{Team: Paris_Saclay/notebook_header}} | {{Team: Paris_Saclay/notebook_header}} | ||
| − | = | + | =Thursday 23rd June= |
==Lab work== | ==Lab work== | ||
| − | === | + | ====Competent cells==== |
| − | ==== | + | ''by Caroline and Lea'' |
| − | ''by '' | + | |
| + | A transformation was made with Interlab study constructions using the same protocol as 22/06/2016 but this time with our competent cells. A negative control and pSB1C3 blue plasmids (positive control) were also transformed and displayed. | ||
| + | |||
| + | |||
| + | ===Interlab study=== | ||
| + | ====Transformation results==== | ||
| + | ''by Caroline, Lea and Marion'' | ||
| + | |||
| + | Colonies were observed in all conditions except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely. | ||
| + | |||
| + | ====Transformed cell cultures==== | ||
| + | One colony was taken into 3,5mL LB medium + Cm at 30µg/mL for each condition (except of course for device 1). | ||
{{Team: Paris_Saclay/notebook_footer}} | {{Team: Paris_Saclay/notebook_footer}} | ||
Revision as of 12:38, 4 July 2016
Template:Team: Paris Saclay/notebook header
Contents
Thursday 23rd June
Lab work
Competent cells
by Caroline and Lea
A transformation was made with Interlab study constructions using the same protocol as 22/06/2016 but this time with our competent cells. A negative control and pSB1C3 blue plasmids (positive control) were also transformed and displayed.
Interlab study
Transformation results
by Caroline, Lea and Marion
Colonies were observed in all conditions except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.
Transformed cell cultures
One colony was taken into 3,5mL LB medium + Cm at 30µg/mL for each condition (except of course for device 1).
