Difference between revisions of "Team:Paris Saclay/Notebook/June/28"

(Created page with "{{Team: Paris_Saclay/notebook_header}} =Tuesday 28th June= ==Lab work== ===Visualization=== ====Puc19 digestion==== <add picture> Puc19 was digested as expected. ====Puc19-...")
 
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===Visualization===
 
===Visualization===
 
====Puc19 digestion====  
 
====Puc19 digestion====  
−
<add picture>
+
<!--add picture-->
 
Puc19 was digested as expected.
 
Puc19 was digested as expected.
  
 
====Puc19-gBlocks ligation====  
 
====Puc19-gBlocks ligation====  
 
''By Charlene''
 
''By Charlene''
 +
GBlocks were resuspended into 100µL of TE buffer (except for 4-1 which was resuspended into 50µL of TE) in order to get a concentration of 10ng/mL after a quick spin.
 +
Solutions were vortexed, incubated for 20min at 50°C, vortexed another time and quickly spun down.
  
 +
{| class="wikitable"
 +
|-
 +
!'''gBlock'''
 +
!1-1
 +
!1-2
 +
!2-1
 +
!3-1
 +
!3-2
 +
!4-1
 +
!4-2
 +
!GFP1-9
 +
|-
 +
|'''Size (bp)'''
 +
|960
 +
|960
 +
|1023
 +
|960
 +
|960
 +
|706
 +
|1288
 +
|862
 +
|-
 +
|'''vector size/insert size x 3.5'''
 +
|9.84
 +
|9.84
 +
|9.24
 +
|9.84
 +
|9.84
 +
|13.39
 +
|7.34
 +
|10.96
 +
|}
 +
 +
3 controls were made :
 +
# 1µL digested vector + 9µL H2O
 +
# 1µL digested vector + 1µL ligase + 1µL ligation buffer + 7µL H2O
 +
# 1µL digested vector + 1µL ligase + 1µL ligation buffer + 7µL insert
  
 
{{Team: Paris_Saclay/notebook_footer}}
 
{{Team: Paris_Saclay/notebook_footer}}

Revision as of 14:22, 4 July 2016

Template:Team: Paris Saclay/notebook header

Tuesday 28th June

Lab work

Visualization

Puc19 digestion

Puc19 was digested as expected.

Puc19-gBlocks ligation

By Charlene GBlocks were resuspended into 100µL of TE buffer (except for 4-1 which was resuspended into 50µL of TE) in order to get a concentration of 10ng/mL after a quick spin. Solutions were vortexed, incubated for 20min at 50°C, vortexed another time and quickly spun down.

gBlock 1-1 1-2 2-1 3-1 3-2 4-1 4-2 GFP1-9
Size (bp) 960 960 1023 960 960 706 1288 862
vector size/insert size x 3.5 9.84 9.84 9.24 9.84 9.84 13.39 7.34 10.96

3 controls were made :

  1. 1µL digested vector + 9µL H2O
  2. 1µL digested vector + 1µL ligase + 1µL ligation buffer + 7µL H2O
  3. 1µL digested vector + 1µL ligase + 1µL ligation buffer + 7µL insert

Template:Team: Paris Saclay/notebook footer