Difference between revisions of "Team:Paris Saclay/Notebook/June/22"
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Revision as of 15:09, 4 July 2016
Template:Team: Paris Saclay/notebook header
Contents
Wednesday 22ond June
Lab work
Competent cells
by Marion
At 10am OD was 0.3. At 1pm OD was 0.46. At 3pm OD was 0.62.
The solution was put on ice 10min (stop the OD). After, it was centrifuged 10min at 4°C at 3000rpm. The pellet was resuspended in 80mL ice-cold TB and incubated on ice for 10min. The solution was centrifuged again at 4°C at 3000rpm. The pellet was resuspended in 20mL ice-cold TB and DMSO was added at 7% of the final concentration (1.58mL of DMSO for 21mL of TB+cells). The solution was incubated on ice for 10min and then distributed in tubes, frozen in liquid nitrogen and stored at -70°C.
Interlab study
Transformation
by Lea and Caroline
No colony grew on the petri dish. Transformations have to be redone. Same protocol as 21/06/2016 was followed. But this time 2µL of plasmids were taken, a new solution of antibiotic Cm was used and a positive control was added (a pSB1C3 plasmid).
