Difference between revisions of "Team:USP UNIFESP-Brazil/Interlab"

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Imagine you are a Biohacker or someone very interested in Science stuff, but you have no money… How could you avoid expensive high-end equipment and yet, still obtain some data about the promoters you love?  
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Imagine you are a Biohacker or someone very interested in Science stuff, but you have no money… How could you avoid expensive high-end equipment and yet, still obtain some data about the promoters you love?  
 
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    In order to do so and draw a sketch of our promoter’s strength we have followed and updated the 2015 <a href="https://2015.igem.org/Team:Brasil-USP/interlabstudy">USP_Brazil iGEM team approach</a> for an inexpensive and quick analysis by taking digital photos and analyzing them on open-source softwares for image processing (<a href="https://www.gimp.org/">GIMP</a> and <a href="https://imagej.nih.gov/ij/">ImageJ</a>)
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In order to do so and draw a sketch of our promoter’s strength we have followed and updated the 2015 <a href="https://2015.igem.org/Team:Brasil-USP/interlabstudy">USP_Brazil iGEM team approach</a> for an inexpensive and quick analysis by taking digital photos and analyzing them on open-source softwares for image processing (<a href="https://www.gimp.org/">GIMP</a> and <a href="https://imagej.nih.gov/ij/">ImageJ</a>)
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All test devices and controls were grown on both solid (LB-Agar) and Liquid (LB and M9) media and photos were taken by a regular cellphone under the effect of fluorescent white or blue light lamps (for exciting GFP reporter molecules). The choice of comparing both LB and M9 liquid media was based on an extensive number of reports regarding the influence of auto fluorescence of LB on measurements. Thus, we wanted to have check if the outcome of this effect would be so strong that it could be visually detected.
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Revision as of 16:29, 17 October 2016

AlgAranha Team USP-UNIFESP BRASIL

iGEM 2016

MACROSCOPIC ANALYSIS

Imagine you are a Biohacker or someone very interested in Science stuff, but you have no money… How could you avoid expensive high-end equipment and yet, still obtain some data about the promoters you love?

In order to do so and draw a sketch of our promoter’s strength we have followed and updated the 2015 USP_Brazil iGEM team approach for an inexpensive and quick analysis by taking digital photos and analyzing them on open-source softwares for image processing (GIMP and ImageJ)

All test devices and controls were grown on both solid (LB-Agar) and Liquid (LB and M9) media and photos were taken by a regular cellphone under the effect of fluorescent white or blue light lamps (for exciting GFP reporter molecules). The choice of comparing both LB and M9 liquid media was based on an extensive number of reports regarding the influence of auto fluorescence of LB on measurements. Thus, we wanted to have check if the outcome of this effect would be so strong that it could be visually detected.