Difference between revisions of "Team:IIT Kharagpur/Notebook"

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{{IIT_Kharagpur}}
+
<html lang="en-US">
−
<html>
+
<head>
 +
<title>IGEM-IIT Kharagpur- Notebook</title>
  
  
−
<div class="column full_size">
 
  
−
<p> Document the dates you worked on your project.</p>
 
  
−
</div>
+
 +
<meta charset="utf-8">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
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<link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/notebook_bootstrap?action=raw&ctype=text/css" />
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<link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/notebook_font_awesome_min?action=raw&ctype=text/css" />
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<link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/notebook_fancy_buttons?action=raw&ctype=text/css" />
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<link href='http://fonts.googleapis.com/css?family=Bangers' rel='stylesheet' type='text/css'>
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<link href='http://fonts.googleapis.com/css?family=Roboto+Slab:300,700,400' rel='stylesheet' type='text/css'>
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<link href='http://fonts.googleapis.com/css?family=Raleway:600,400,300' rel='stylesheet' type='text/css'>
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<link href='http://fonts.googleapis.com/css?family=Open+Sans:400,300,600,700' rel='stylesheet' type='text/css'>
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<link href="http://fonts.googleapis.com/css?family=Roboto+Condensed:400,300,700" rel="stylesheet" type="text/css" />
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<![endif] -->
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margin-top: -3%;
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height: 23px;
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width: auto;
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margin-right: 5px;
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#section-footer{
 +
padding-top: 10px;
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padding-bottom: 10px;
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font-family: "Helvetica Neue",Helvetica,Arial,sans-serif;
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color: white;
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}
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#logokgp{
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table {
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text-decoration: none;
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header a {
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  display: inline-block;
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header a:hover {
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}
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[role="main"] {
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  overflow: hidden;
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  font-family: "Helvetica";
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}
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section ul {
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  list-style-type: none;
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}
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section li {
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  position: relative;
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  display: inline-block;
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}
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}
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}
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 +
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}
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 +
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}
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 +
width: 220px;
 +
padding-top: 15px;
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}
 +
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 +
color: white;
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padding-top: 10px;
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font-family: 'Dosis';
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font-style: normal;
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font-weight: 300;
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display: block;
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clear: both;
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box-sizing: border-box;
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 +
body, html {
 +
height: 100%;
 +
}
 +
 
 +
body {
 +
background: #f9f9f9;
 +
background-size: cover;
 +
margin: 0;
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padding: 0;
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font-family: helvetica, arial, tahoma, verdana;
 +
line-height: 20px;
 +
font-size: 14px;
 +
color: #726f77;
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img {
 +
max-width: 100%;
 +
}
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 +
a {
 +
text-decoration: none;
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}
 +
 
 +
.container {
 +
max-width: 1100px;
 +
padding-top: 10px;
 +
margin: 0 auto;
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}
 +
 
 +
h1, h2, h3, h4 {
 +
font-family: "Dosis", arial, tahoma, verdana;
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font-weight: 500;
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 +
text-align: center;
 +
padding: 10px 0;
 +
}
 +
 
 +
header {
 +
background: #2b2e48;
 +
padding: 10px;
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box-shadow: 0 3px 3px rgba(0, 0, 0, 0.05);
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header .logo {
 +
color: #ee4d4d;
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float: left;
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font-family: "Dosis", arial, tahoma, verdana;
 +
font-size: 22px;
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font-weight: 500;
 +
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 +
 
 +
header .logo > span {
 +
color: #f7aaaa;
 +
font-weight: 300;
 +
}
 +
 
 +
header .social {
 +
float: right;
 +
}
 +
 
 +
header .social .btn {
 +
font-family: "Dosis";
 +
font-size: 14px;
 +
margin: 10px 5px;
 +
}
 +
 
 +
#timeline {
 +
width: 100%;
 +
margin: 10px auto;
 +
position: relative;
 +
padding: 0 5px;
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-webkit-transition: all 0.4s ease;
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-moz-transition: all 0.4s ease;
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-ms-transition: all 0.4s ease;
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transition: all 0.4s ease;
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z-index: 250;
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}
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 +
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 +
content: "";
 +
width: 3px;
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height: 100%;
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background: #ee4d4d;
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left: 50%;
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top: 0;
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position: absolute;
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}
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 +
#timeline:after {
 +
content: "";
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clear: both;
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display: table;
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width: 100%;
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}
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#timeline .timeline-item {
 +
margin-bottom: 50px;
 +
position: relative;
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}
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 +
#timeline .timeline-item .timeline-icon {
 +
background: #ee4d4d;
 +
width: 50px;
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height: 50px;
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position: absolute;
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top: 0;
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left: 50%;
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overflow: hidden;
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margin-left: -23px;
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-webkit-border-radius: 50%;
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-moz-border-radius: 50%;
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-ms-border-radius: 50%;
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border-radius: 50%;
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}
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 +
#timeline .timeline-item .timeline-icon svg {
 +
position: relative;
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top: 14px;
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left: 14px;
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}
 +
 
 +
#timeline .timeline-item .timeline-content {
 +
width: 45%;
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background: #fff;
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padding: 20px;
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box-shadow: 0 3px 0 rgba(0, 0, 0, 0.1);
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-moz-border-radius: 5px;
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-ms-border-radius: 5px;
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border-radius: 5px;
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-webkit-transition: all 0.3s ease;
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-moz-transition: all 0.3s ease;
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-ms-transition: all 0.3s ease;
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transition: all 0.3s ease;
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}
 +
 
 +
#timeline .timeline-item .timeline-content h2 {
 +
padding: 15px;
 +
background: #ee4d4d;
 +
color: #fff;
 +
margin: -20px -20px 0 -20px;
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font-weight: 300;
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-webkit-border-radius: 3px 3px 0 0;
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-moz-border-radius: 3px 3px 0 0;
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-ms-border-radius: 3px 3px 0 0;
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border-radius: 3px 3px 0 0;
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}
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#timeline .timeline-item .timeline-content:before {
 +
content: '';
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position: absolute;
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left: 45%;
 +
top: 20px;
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width: 0;
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height: 0;
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border-top: 7px solid transparent;
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border-bottom: 7px solid transparent;
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border-left: 7px solid #ee4d4d;
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}
 +
 
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#timeline .timeline-item .timeline-content.right {
 +
float: right;
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}
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#timeline .timeline-item .timeline-content.right:before {
 +
content: '';
 +
right: 45%;
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left: inherit;
 +
border-left: 0;
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border-right: 7px solid #ee4d4d;
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}
 +
 
 +
.btn {
 +
padding: 5px 15px;
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text-decoration: none;
 +
background: transparent;
 +
border: 2px solid #f27c7c;
 +
color: #f27c7c;
 +
display: inline-block;
 +
position: relative;
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text-transform: uppercase;
 +
font-size: 12px;
 +
-webkit-border-radius: 5px;
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-moz-border-radius: 5px;
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-ms-border-radius: 5px;
 +
border-radius: 5px;
 +
-webkit-transition: background 0.3s ease;
 +
-moz-transition: background 0.3s ease;
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-ms-transition: background 0.3s ease;
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transition: background 0.3s ease;
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-webkit-box-shadow: 2px 2px 0 #f27c7c;
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-moz-box-shadow: 2px 2px 0 #f27c7c;
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-ms-box-shadow: 2px 2px 0 #f27c7c;
 +
box-shadow: 2px 2px 0 #f27c7c;
 +
}
 +
 
 +
.btn:hover {
 +
box-shadow: none;
 +
top: 2px;
 +
left: 2px;
 +
-webkit-box-shadow: 2px 2px 0 transparent;
 +
-moz-box-shadow: 2px 2px 0 transparent;
 +
-ms-box-shadow: 2px 2px 0 transparent;
 +
box-shadow: 2px 2px 0 transparent;
 +
}
 +
 
 +
@media screen and (max-width: 768px) {
 +
#timeline {
 +
margin: 30px;
 +
padding: 0px;
 +
width: 90%;
 +
}
 +
 
 +
#timeline:before {
 +
left: 0;
 +
}
 +
 
 +
#timeline .timeline-item .timeline-content {
 +
width: 90%;
 +
float: right;
 +
}
 +
 
 +
#timeline .timeline-item .timeline-content:before, #timeline .timeline-item .timeline-content.right:before {
 +
left: 10%;
 +
margin-left: -6px;
 +
border-left: 0;
 +
border-right: 7px solid #ee4d4d;
 +
}
 +
 
 +
#timeline .timeline-item .timeline-icon {
 +
left: 0;
 +
}
 +
}
 +
 
 +
.calendar{
 +
background-color: #e0e0e0;
 +
padding-bottom: 5px;
 +
position: relative;
 +
z-index: 500;
 +
}
 +
.notebook-content{
 +
background-color: #f9f9f9;
 +
z-index: 250;
 +
position: relative;
 +
padding-top: 280px;
 +
}
 +
.topcontainer{
 +
color: white;
 +
z-index: 12;
 +
position: relative;
 +
}
 +
 
 +
 
 +
#sticky.stick {
 +
    margin-top: 0 !important;
 +
    position: fixed;
 +
    top: 0;
 +
    z-index: 10000;
 +
    border-radius: 0 0 0.5em 0.5em;
 +
}
 +
.distance{
 +
padding-top: 5px;
 +
}
 +
.bluecolor{
 +
background-color: #297b6e;
 +
}
 +
a{
 +
position: relative;
 +
top: -5px;
 +
}
 +
.relativeposition{
 +
position: relative;
 +
top: -5px;
 +
 
 +
}
 +
.affix{
 +
position: fixed;
 +
top: 30px;
 +
 
 +
}
 +
.footerimage{
 +
  width: 100px;
 +
  height: auto;
 +
}
 +
.dropbtn {
 +
    background-color: #4CAF50;
 +
    color: white;
 +
    padding: 16px;
 +
    font-size: 16px;
 +
    border: none;
 +
    cursor: pointer;
 +
}
 +
 
 +
.dropdown {
 +
    position: relative;
 +
    display: inline-block;
 +
}
 +
 
 +
.dropdown-content {
 +
    display: none;
 +
    position: absolute;
 +
    background-color: #f9f9f9;
 +
    min-width: 160px;
 +
    box-shadow: 0px 8px 16px 0px rgba(0,0,0,0.2);
 +
}
 +
 
 +
.dropdown-content a {
 +
    color: black;
 +
    padding: 12px 16px;
 +
    text-decoration: none;
 +
    display: block;
 +
}
 +
 
 +
.dropdown-content a:hover {background-color: #b04545}
 +
 
 +
.dropdown:hover .dropdown-content {
 +
    display: block;
 +
}
 +
 
 +
.dropdown:hover .dropbtn {
 +
    background-color: #3e8e41;
 +
}
 +
.igem-logo-top{
 +
  width: 55px;
 +
  height: auto;
 +
 
 +
  float: left;
 +
  position: relative;
 +
  right: 12px;
 +
}
 +
#mail{
 +
background-color: #17bcb8 !important;
 +
-webkit-tap-highlight-color: transparent;
 +
    will-change: opacity, transform;
 +
    height: 40px;
 +
    width: 80px;
 +
    margin-top: -10px;
 +
    font-size: 14px;
 +
    font-weight: 400;
 +
    font-family: 'Dosis';
 +
    border-style: none;
 +
    color: black;
 +
    box-shadow: none;
 +
}
 +
.social{
 +
height: 40px;
 +
width: auto;
 +
}
 +
.logobody{
 +
margin-left: 4%;
 +
 
 +
}
 +
.home_content{
 +
margin-top: -2%;
 +
}
 +
.table{
 +
width: 200px;
 +
height: auto;
 +
 +
position: absolute;
 +
margin-left:-7.5em;
 +
margin-top: -1%;
 +
}
 +
.tabledown{
 +
position: relative;
 +
    top: 15em;
 +
}
 +
.well-come{
 +
margin-top: 4em;
 +
}
 +
.center_icon{
 +
width: 200px;
 +
height: auto;
 +
 +
position: absolute;
 +
margin-left:-7.5em;
 +
margin-top: -1%;
 +
}
 +
 
 +
 
 +
 
 +
</style>
 +
 
 +
 
 +
</head>
 +
<body >
 +
<!--=== Preloader section starts ===-->
 +
<section id="preloader">
 +
<div class="loading-circle fa-spin"></div>
 +
</section>
 +
<!--=== Preloader section Ends ===-->
 +
 +
<!--=== Header section Starts ===-->
 +
  <div id="header" class="header-section">
 +
    <!-- sticky-bar Starts-->
 +
<div class="sticky-bar-wrap">
 +
<div class="sticky-section">
 +
<div id="topbar-hold" class="nav-hold container">
 +
<img src="https://static.igem.org/mediawiki/2016/thumb/8/86/T--IIT_Kharagpur--logo.png/180px-T--IIT_Kharagpur--logo.png" class="igem-logo-top">
 +
<nav class="navbar" role="navigation">
 +
<div class="navbar-header">
 +
<button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-responsive-collapse">
 +
<span class="sr-only">Toggle navigation</span>
 +
<span class="icon-bar"></span>
 +
<span class="icon-bar"></span>
 +
<span class="icon-bar"></span>
 +
</button>
 +
<!--=== Site Name ===-->
 +
<a class="site-name navbar-brand" href="https://2016.igem.org/Team:IIT_Kharagpur"><span>IIT</span>Kharagpur</a>
 +
</div>
 +
 +
<!-- Main Navigation menu Starts -->
 +
<div class="collapse navbar-collapse navbar-responsive-collapse">
 +
<ul class="nav navbar-nav navbar-right">
 +
<li class="current dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur"><img src="https://static.igem.org/mediawiki/2016/1/13/T--IIT_Kharagpur--home_icon.png" class="icon"> Home</a>
 +
 +
</li>
 +
<li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Project"><img src="https://static.igem.org/mediawiki/2016/5/55/T--IIT_Kharagpur--project_icon.png" class="icon">Project</a>
 +
<div class="dropdown-content">
 +
    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Notebook" class="submenuhover">Notebook</a>
 +
<a href="https://2016.igem.org/Team:IIT_Kharagpur/Protocol" class="submenuhover">Protocol</a>
 +
    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Data_page" class="submenuhover">Data Page</a>
 +
<a href="https://2016.igem.org/Team:IIT_Kharagpur/Safety" class="submenuhover">Safety</a>
 +
  </div>
 +
 
 +
</li>
 +
<li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Team"><img src="https://static.igem.org/mediawiki/2016/c/c1/T--IIT_Kharagpur--team_icon.png" class="icon">Team</a>
 +
<div class="dropdown-content">
 +
    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Team" class="submenuhover">Members</a>
 +
<a href="https://2016.igem.org/Team:IIT_Kharagpur/Collaborations" class="submenuhover">Collaboration</a>
 +
    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Attributions" class="submenuhover">Attribution</a>
 +
 +
  </div>
 +
</li >
 +
<li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/9/9f/T--IIT_Kharagpur--practice_icon.png" class="icon">Practices</a>
 +
<div class="dropdown-content">
 +
    <a href="#" class="submenuhover">Survey</a>
 +
<a href="#" class="submenuhover">Workshop</a>
 +
    <a href="#" class="submenuhover">Interview</a>
 +
<a href="#" class="submenuhover">Meet ups</a>
 +
  </div>
 +
 
 +
 
 +
</li>
 +
<li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts"><img src="https://static.igem.org/mediawiki/2016/7/76/T--IIT_Kharagpur--part_icon.png" class="icon">Part</a>
 +
<div class="dropdown-content">
 +
    <a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts" class="submenuhover">Composite parts</a>
 +
<a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts" class="submenuhover">Part collection </a>
 +
   
 +
  </div>
 +
 
 +
</li>
 +
 +
<li><a href="https://2016.igem.org/Team:IIT_Kharagpur/Interlab_Study"><img src="https://static.igem.org/mediawiki/2016/d/df/T--IIT_Kharagpur--interlab_icon.png" class="icon">Interlab</a></li>
 +
<li><a href="blog.html"><img src="https://static.igem.org/mediawiki/2016/5/5e/T--IIT_Kharagpur--blog_icon.png" class="icon">Blog</a></li>
 +
 +
 
 +
</div>
 +
<!-- Main Navigation menu ends-->
 +
 
 +
 
 +
</nav>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- sticky-bar Ends-->
 +
<!--=== Header section Ends ===-->
 +
 +
<!--=== Home Section Starts ===-->
 +
<div id="section-home" class="home-section-wrap center">
 +
<div class="section-overlay"></div>
 +
<div class="topcontainer home">
 +
 +
<div class="row">
 +
<div class="home_content">
 +
 +
<h1 class="well-come" style="font-family:Dosis;">PROTOCOL</h1>
 +
<img src="https://static.igem.org/mediawiki/2016/7/74/T--IIT_Kharagpur--protocol_centre.png" class="center_icon">
 +
 +
<div class="col-md-8 col-md-offset-2 tabledown">
 +
<p class="intro-message" style="font-family:Dosis; font-size:20px; text-align:center;">HARD WORK JUST LIKE JUST MA AND PA TAUGHT US</p>
 +
</div>
 +
<div class="home-buttons">
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!--=== Home Section Ends ===-->
 +
</div>
 +
 
 +
 +
 
 +
 
 +
<div class="calendar col-md-12" id="myaffix" hidden-xs hidden-sm hidden-md data-spy="affix" data-offset-top="730">
 +
<div class="calendar col-md-3 col-sm-3"  >
 +
<div class="calendar-patch">
 +
<header role="banner">
 +
      <time>July<em>2016</em></time>
 +
    </header>
 +
    <section role="main">
 +
      <ul class="m-box--weeks">
 +
       
 +
        <li>
 +
          Mon
 +
        </li>
 +
        <li>
 +
          Tue
 +
        </li>
 +
        <li>
 +
          Wed
 +
        </li>
 +
        <li>
 +
          Thu
 +
        </li>
 +
        <li>
 +
          Fri
 +
        </li>
 +
        <li>
 +
          Sat
 +
        </li>
 +
        <li>
 +
          Sun
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">27</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">28</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">29</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">30</p>
 +
        </li>
 +
        <li >
 +
          <p class="relativeposition">1</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">2</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">3</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li>
 +
          <p class="relativeposition">4</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">5</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">6</p>
 +
        </li>
 +
        <li class="l-date--event" data-event="15:00 - New Haircut">
 +
          <i class="m-bullet--event"></i> 7
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">8</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">9</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">10</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li>
 +
          <p class="relativeposition"> 11</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">12</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">13</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">14</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">15</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">16</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">17</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li>
 +
          <p class="relativeposition">18</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">19</p>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#20july">20</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#21july">21</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#22july">22</a>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">23</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">24</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#july25">25</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a  data-scroll href="#july26">26</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a  data-scroll href="#july27">27</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#july28">28</a>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">29</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">30</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition"> 31</p>
 +
        </li>
 +
      </ul>
 +
        <ul class="m-box--date">
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">1</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">2</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">3</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">4</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">5</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">6</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">7</p>
 +
        </li>
 +
      </ul>
 +
    </section>
 +
</div>
 +
</div>
 +
 
 +
<div class="calendar col-md-3 col-sm-3" hidden-xs hidden-sm hidden-md >
 +
<div class="calendar-patch">
 +
<header role="banner">
 +
      <time>August<em>2016</em></time>
 +
    </header>
 +
    <section role="main">
 +
      <ul class="m-box--weeks">
 +
       
 +
        <li>
 +
          Mon
 +
        </li>
 +
        <li>
 +
          Tue
 +
        </li>
 +
        <li>
 +
          Wed
 +
        </li>
 +
        <li>
 +
          Thu
 +
        </li>
 +
        <li>
 +
          Fri
 +
        </li>
 +
        <li>
 +
          Sat
 +
        </li>
 +
        <li>
 +
          Sun
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">25</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">26</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">27</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">28</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">29</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">30</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">31</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="bluecolor">
 +
        <a data-scroll  href="#august1"> 1</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august2"> 2</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august3"> 3</a>
 +
        </li>
 +
        <li  class="bluecolor">
 +
          <a data-scroll  href="#august4"> 4</a>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">5</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">6</p>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august7">7</a>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august8">8</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august9">9</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august10">10</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august11">11</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august12">12</a>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">13</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">14</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li>
 +
          <p class="relativeposition">15</p>
 +
        </li>
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august16">16</a></li></p>
 +
       
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august17">17</a></li></p>
 +
     
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august18">18</a></li></p>
 +
       
 +
        <li>
 +
          <p class="relativeposition">19</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">20</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">21</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august22">22</a></li></p>   
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august23">23</a></li></p>
 +
       
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august24">24</a></li></p>
 +
       
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august25">25</a></li></p>
 +
       
 +
       
 +
          <li class="bluecolor">
 +
          <a data-scroll  href="#august26">26</a></li></p>
 +
       
 +
        <li>
 +
          27
 +
        </li>
 +
        <li>
 +
          28
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august29">29</a>
 +
        </li>
 +
        <li>
 +
          30
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#august31">31</a>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">1</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">2</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">3</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">4</p>
 +
        </li>
 +
      </ul>
 +
    </section>
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
<div class="calendar col-md-3 col-sm-3" >
 +
<div class="calendar-patch">
 +
<header role="banner">
 +
      <time>September<em>2016</em></time>
 +
    </header>
 +
    <section role="main">
 +
      <ul class="m-box--weeks">
 +
       
 +
        <li>
 +
          <p class="relativeposition">Mon</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">Tue</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">Wed</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">Thu</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">Fri</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">Sat</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">Sun</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">29</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">30</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">31</p>
 +
        </li>
 +
      <li class="bluecolor">
 +
          <a data-scroll href="#september1">1</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september2">2</a>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">3</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">4</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li>
 +
          <p class="relativeposition">5</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">6</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">7</p>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september8">8</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september9">9</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september10">10</a>
 +
        </li>
 +
        <li>
 +
          11
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september12">12</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september13">13</a>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">14</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">15</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">16</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">17</p>
 +
        </li>
 +
        <li>
 +
          <p class="relativeposition">18</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september19">19</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september20">20</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september21">21</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september22">22</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september23">23</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september24">24</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september25">25</a>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
      <li class="bluecolor">
 +
          <a data-scroll  href="#september26">26</a>
 +
        </li>
 +
      <li class="bluecolor">
 +
          <a data-scroll  href="#september27">27</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september28">28</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september29">29</a>
 +
        </li>
 +
        <li class="bluecolor">
 +
          <a data-scroll  href="#september30">30</a>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">1</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">2</p>
 +
        </li>
 +
      </ul>
 +
      <ul class="m-box--date">
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">3</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">4</p>
 +
        </li>
 +
        <li  class="l-date--passed">
 +
          <p class="relativeposition">5</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">6</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">7</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">8</p>
 +
        </li>
 +
        <li class="l-date--passed">
 +
          <p class="relativeposition">9</p>
 +
        </li>
 +
      </ul>
 +
    </section>
 +
</div>
 +
</div>
 +
 
 +
 +
 
 +
 
 +
 
 +
<div class="calendar col-md-3 col-sm-3">
 +
<div class="calendar-patch">
 +
<header role="banner">
 +
      <time>October<em>2016</em></time>
 +
    </header>
 +
    <section role="main">
 +
      <ul class="m-box--weeks">
 +
       
 +
        <li>    <p class="relativeposition">      Mon </p>        </li>        <li>      <p class="relativeposition">    Tue</p>
 +
</li>        <li>        <p class="relativeposition">  Wed </p>        </li>        <li>          <p class="relativeposition">Thu</p>
 +
</li>        <li>        <p class="relativeposition">  Fri    </p>    </li>        <li>          <p class="relativeposition">Sat</p>
 +
</li>        <li>          <p class="relativeposition"> Sun    </p>    </li>      </ul>      <ul
 +
class="m-box--date">        <li class="l-date--passed">          <p class="relativeposition"> 26</p>
 +
</li>        <li class="l-date--passed">          <p class="relativeposition">27    </p>    </li>
 +
<li class="l-date--passed">          <p class="relativeposition">28        </p> </li>        <li
 +
class="l-date--passed">          <p class="relativeposition"> 29  </p>    </p>  </li>        <li class="l-date--
 +
passed">          <p class="relativeposition"> 30  </p>      </li>        <li class="bluecolor">
 +
          <a data-scroll  href="#october1">1</a>
 +
        </li>
 +
<li class="bluecolor">
 +
          <a data-scroll  href="#october2">2</a>
 +
        </li>
 +
              </ul>
 +
                      <ul class="m-box--date">
 +
<li>          3        </li>        <li>          4        </li>
 +
<li>          5        </li>        <li class="l-date--event" data-
 +
event="15:00 - New Haircut">          <i class="m-bullet--event"></i> 6
 +
</li>        <li>          7        </li>        <li>          8
 +
</li>        <li>          9        </li>      </ul>      <ul
 +
class="m-box--date">        <li>          10        </li>        <li>
 +
11        </li>        <li>          12        </li>        <li>
 +
13        </li>        <li>          14        </li>        <li>
 +
15        </li>        <li>          16        </li>      </ul>      <ul
 +
class="m-box--date">        <li>          17        </li>        <li>
 +
18        </li>        <li>          19        </li>        <li>
 +
20        </li>        <li>          21        </li>        <li>
 +
22        </li>        <li>          23        </li>      </ul>      <ul
 +
class="m-box--date">        <li>          24        </li>        <li>
 +
25        </li>        <li>          26        </li>        <li>
 +
27        </li>        <li>          28        </li>        <li>
 +
29        </li>        <li>          30        </li>      </ul>      <ul
 +
class="m-box--date">        <li>          31        </li>        <li
 +
class="l-date--passed">          1        </li>        <li class="l-date--
 +
passed">          2        </li>        <li class="l-date--passed">
 +
3        </li>        <li class="l-date--passed">          4        </li>
 +
<li class="l-date--passed">          5        </li>        <li
 +
class="l-date--passed">          6        </li>      </ul>    </section>
 +
</div>    </div>
 +
 
 +
  
−
<div class="column half_size">
 
−
<h5>What should this page have?</h5>
 
−
<ul>
 
−
<li>Chronological notes of what your team is doing.</li>
 
−
<li> Brief descriptions of daily important events.</li>
 
−
<li>Pictures of your progress. </li>
 
−
<li>Mention who participated in what task.</li>
 
−
</ul>
 
  
 
</div>
 
</div>
  
−
<div class="column half_size">
 
−
<h5>Inspiration</h5>
 
−
<p>You can see what others teams have done to organize their notes:</p>
 
  
−
<ul>  
+
 
−
<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
+
<div class="notebook-content">
−
<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
+
 
−
<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
+
 
−
<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
+
 
−
</ul>
+
 
 +
 
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK ONE - ( 20th July - 22th July )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="20july">
 +
<h2>20th july, Wednesday</h2>
 +
<p class="distance">
 +
DH5 alpha competent cells were prepared for transformation experiments.
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="21july">
 +
<h2>21st July, Thursday</h2>
 +
<p>
 +
Transformation of the prepared competent cells was carried out.
 +
The plasmids used for transformation  were YFP (BBa_K592101), HIV cleavage site (BBa_I712015), and the protease construct (BBa_K743013)
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="22july">
 +
<h2>22nd July, Friday</h2>
 +
<p>
 +
Replication plating for above transformed parts was done. This including the three sets of  transformed cells containing the plasmids YFP (BBa_K592101), the HIV cleavage site (BBa_I712015), and the protease construct (BBa_K743013).
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
 
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK TWO - ( 25th July - 30th July )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="july25">
 +
<h2>25th july, Monday</h2>
 +
<p class="distance">
 +
1 litre of LB media was prepared and duly autoclaved.
 +
5 plates each of the antibiotics chloramphenicol, ampicillin and ampicillin+Kanamycin were prepared.
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="july26">
 +
<h2>26th July, Tuesday</h2>
 +
<p>
 +
20ml Cultures were prepared for the plasmid extraction of each of YFP (BBa_K592101), the HIV cleavage site (BBa_I712015) and the protease construct (BBa_K743013).
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="july27">
 +
<h2>27th July, Wednesday</h2>
 +
<p>
 +
Plasmid  extraction was done for  YFP (BBa_K592101), the HIV cleavage site (BBa_I712015), and the protease construct (BBa_K743013) using mini-prep plasmid DNA extraction kit.
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="july28">
 +
<h2>28th July, Thursday</h2>
 +
<p>
 +
DNA quantification was done using Nano Drop.
 +
YFP: 250 ng/ul<br>
 +
Protease: 170 ng/ul<br>
 +
Cleavage Site: 120 ng/ul<br>
 +
1% agarose gel was prepared  for screening of the plasmids extracted by the mini prep kit.<br>
 +
The FRET construct was kept for overnight digestion at the restriction sites E and P.
 +
<br>
 +
Digestions with E and P for YFP, Protease and Cleavage Site<table>
 +
<tr><th></th><th>YFP</th><th>Protease</th><th>Cleavage Site</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th><th>Volume(ul)</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>2</td><td>3</td><td>3.5</td></tr>
 +
<tr><td>2.1 Buffer</td><td>2</td><td>2</td><td>2</td></tr>
 +
<tr><td>ECoRI</td><td>0.5</td><td>0.5</td><td>0.5</td></tr>
 +
<tr><td>Psti</td><td>0.5</td><td>0.5</td><td>-</td></tr>
 +
<tr><td>Nf H2O</td><td>15</td><td>14</td><td>14</td></tr>
 +
</table>
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK THREE - ( 1st August - 7th August )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august1">
 +
<h2>1st August, Monday</h2>
 +
<p class="distance">
 +
3ml cultures made for the preparation of competent cells of DH5 alpha cells.
 +
20ml culture prepared for the construct BBa_K844004. (MaSP without ATG)
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august2">
 +
<h2>2nd August, Tuesday</h2>
 +
<p>
 +
11 vials of DH5 alpha competent cells were prepared.
 +
Plasmid extraction done for the construct BBa_K844004 (MaSP without ATG).
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august3">
 +
<h2>3rd August, Wednesday</h2>
 +
<p>
 +
As a part of the iGEM 2016 interlab study, transformations were done for the following:<br>
 +
Test device1<br>
 +
Test device2<br>
 +
Test device3<br>
 +
Positive control<br>
 +
Negative control<br>
 +
Also, transformed cells were prepared with the part BBa_K844008 (MaSP with ATG)
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august4">
 +
<h2>4th August, Thursday</h2>
 +
<p>
 +
Colonies were seen for all six of the transformed plates. The transformation procedure was hence successful.
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august7">
 +
<h2>7th August, Sunday</h2>
 +
<p>
 +
20ml Culture was prepared for the screening of BBa_K844008 (MaSP with ATG).
 +
Also, 20ml cultures were prepared for each of test device 1, test device 2, and test device 3 for the interlab study.
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK FOUR - ( 8th August - 12th August )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august8">
 +
<h2>8th August, Monday</h2>
 +
<p class="distance">
 +
PPlasmid extraction performed for the screening of the parts BBa_K844008 (MaSP with ATG); and test device 1, test device 2, test device 3 for the interlab study.
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august9">
 +
<h2>9th August, Tuesday</h2>
 +
<p>
 +
Gel Electrophoresis with 1% agarose gel was done for BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3 for screening.
 +
The run was not successful due to gel over flow.
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august10">
 +
<h2>10th August, Wednesday</h2>
 +
<p>
 +
20ml Culture was prepared for screening of BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3.
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august11">
 +
<h2>11th August, Thursday</h2>
 +
<p>
 +
Plasmid extraction for the screening of BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3 was done.
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august12">
 +
<h2>12th August, Friday</h2>
 +
<p>
 +
Gel electrophoresis with 1% agarose gel was done for BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3.
 +
 
 +
<img src= "pic/T--IIT_Kharagpur--12_aug.jpg" height="200px" width="200px">
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK FIVE - ( 15th August - 18th August )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august16">
 +
<h2>16th August, Tuesday</h2>
 +
<p class="distance">
 +
Plasmid extraction of BBa_K844008 (MaSP with ATG), test device 1, test device 2, and test device 3 was done with mini-prep kit.
 +
Digestion of above obtained plasmids with E And P was carried out.
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august17">
 +
<h2>17th August, Wednesday</h2>
 +
<p>
 +
1% agarose gel was prepared for digested plasmids
 +
PCR of protease construct for HIV site primers was done
 +
PCR parameters:<br>
 +
{95 degree 2 minutes<br>
 +
[95 degree 30 seconds<br>
 +
60 degrees 30 sec<br>
 +
68 degree 2 min] X35 cycles<br>
 +
68 degree 10 min}<br>
 +
 
 +
PCR products run in 1% agarose gel.<br>
 +
Amplification not upto expectation.<br>
 +
Repetition of the experiment, with different conditions suggested.<br>
 +
Note: Too many non specific products were observed that is why the same was repeated next week.
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august18">
 +
<h2>18th August, Thursday</h2>
 +
<p>
 +
PCR of protease construct for HIV site primers was done.<br>
 +
PCR products were run in 1% agarose gel.
 +
<img src= "pic/T--IIT_Kharagpur--18_aug.jpg" height="100px" width="100px"> <br>
 +
 
 +
PCR Parameters:<br>
 +
{95 degree 2 minutes<br>
 +
[95 degree 20 seconds<br>
 +
62 degrees 30 sec<br>
 +
68 degree 2 min 15 sec] X35 cycles<br>
 +
68 degree 10 min}<br>
 +
Amplification not upto expectation.<br>
 +
Repetition of the experiment, with different conditions suggested.
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK SIX - ( 22nd August - 26th August )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august22">
 +
<h2>22th August, Monday</h2>
 +
<p class="distance">
 +
PCR for both fret and silk constructs at two conditions was done.<br>
 +
<img src= "pic/T--IIT_Kharagpur--22_aug.jpg" height="200px" width="200px"> <br>
 +
 +
Condition 1<br>
 +
{95 degree 5 minutes<br>
 +
Taq DNA polymerase is added.<br>
 +
[95 degree 30 seconds<br>
 +
57.5 degrees 40 sec<br>
 +
68 degree 40 sec] X35 cycles<br>
 +
68 degree 10 min}<br>
 +
Condition 2<br>
 +
{95 degree 2 minutes<br>
 +
[95 degree 30 seconds<br>
 +
58 degrees 1 min<br>
 +
68 degree 2 min] X35 cycles<br>
 +
68 degree 10 min}<br>
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august23">
 +
<h2>23rd August, Tuesday</h2>
 +
<p>
 +
PCR for FRET construct was done.
 +
PCR Parameters:<br>
 +
{95 degree 2 minutes<br>
 +
[95 degree 30 seconds<br>
 +
57 degrees 40 sec<br>
 +
68 degree 2 min] X35 cycles<br>
 +
68 degree 10 min}<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august24">
 +
<h2>24th August, Wednesday</h2>
 +
<p>
 +
Gel run for FRET construct was performed.
 +
 
 +
<img src="pic/T--IIT_Kharagpur--24_aug.jpg" height="200px" width="200px">
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august25">
 +
<h2>25th August, Thursday</h2>
 +
<p>
 +
PCR for silk construct was done with the following parameters:<br>
 +
{95 degree 2 minutes<br>
 +
[95 degree 30 seconds<br>
 +
57 degrees 40 sec<br>
 +
68 degree 2 min] X35 cycles<br>
 +
68 degree 10 min}<br>
 +
 
 +
<img src="pic/T--IIT_Kharagpur--25_aug.jpg" height="200px" width="200px">
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august26">
 +
<h2>26th August, Friday</h2>
 +
<p>
 +
PCR for FRET construct was done.<br>
 +
PCR Parameters:<br>
 +
{95 degree 2 minutes<br>
 +
[95 degree 30 seconds<br>
 +
58 degrees 1 min<br>
 +
68 degree 2 min] X35 cycles<br>
 +
68 degree 10 min} <br>
 +
 +
<img src="pic/T--IIT_Kharagpur--26_aug.jpg" height="200px" width="200px">
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK SEVEN - ( 29th August - 2nd September )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="august29">
 +
<h2>29th August, Monday</h2>
 +
<p class="distance">
 +
Nano drop analysis for pSB1K3( Kanamycin backbone) was done.
 +
Result:Obtained concentration was 192.6mg/ul.
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="august31">
 +
<h2>31st August, Wednesday</h2>
 +
<p>
 +
Digestion for Kanamycin backbone and FRET PCR with E And P
 +
<table>
 +
<tr><th></th><th>K backbone</th><th>FRET PCR</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>6</td><td>2.3</td></tr>
 +
<tr><td>2.1 Buffer</td><td>2</td><td>2</td></tr>
 +
<tr><td>ECoRI</td><td>1</td><td>1</td></tr>
 +
<tr><td>Psti</td><td>1</td><td>1</td></tr>
 +
<tr><td>Nf H2O</td><td>10</td><td>13.7</td></tr>
 +
</table>
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september1">
 +
<h2>1st September, Thursday</h2>
 +
<p>
 +
Ligation for Kanamycin backbone and FRET PCR with E and P was done
 +
Vector:Insert=1:3
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september2">
 +
<h2>2nd September, Friday</h2>
 +
<p>
 +
Transformation for Kanamycin backbone and FRET PCR with E and P failed.<br>
 +
 
 +
Note: Reason was determined to be inadequate amount of the PCR product
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK EIGHT - ( 8th September - 10th September )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september8">
 +
<h2>8th September, Thursday</h2>
 +
<p class="distance">
 +
2 ml culture of CFP and YFP was prepared.<br>
 +
 
 +
PCR done for FRET construct and silk construct<br>
 +
Digestion with E and P of FRET PCR product was done.<br>
 +
<table>
 +
<tr><th></th><th>FRET PCR</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>19</td></tr>
 +
<tr><td>2.1 Buffer</td><td>2.5</td></tr>
 +
<tr><td>ECoRI</td><td>1</td></tr>
 +
<tr><td>PstI</td><td>1</td></tr>
 +
<tr><td>NF H2O</td><td>1.5</td></tr>
 +
</table>
 +
 
 +
<img src="pic/T--IIT_Kharagpur--8_sep.jpg" height="200px" width="200px">
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september9">
 +
<h2>9th September, Friday</h2>
 +
<p>
 +
PCR purification of digested FRET PCR product was done.
 +
Plasmid isolation of YFP and CFP was performed.<br>
 +
YFP: 250 ng/ul<br>
 +
CFP: 218 ng/ul<br>
 +
Glycerol stock for CFP and YFP was prepared.<br>
 +
Restriction digestion(overnight) of YFP and cleavage site was done.<br>
 +
<table>
 +
<tr><th></th><th>YFP</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>5</td></tr>
 +
 +
<tr><td>ECoR buffer</td><td>2</td></tr>
 +
<tr><td>ECoRI</td><td>1</td></tr>
 +
<tr><td>NF H2O</td><td>12</td></tr>
 +
</table>
 +
 
 +
<table>
 +
<tr><th></th><th>Cleavage Site</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>10</td></tr>
 +
<tr><td>3.1 Buffer</td><td>2</td></tr>
 +
<tr><td>ECoRI</td><td>1</td></tr>
 +
<tr><td>Xbal</td><td>1</td></tr>
 +
<tr><td>NF H2O</td><td>6</td></tr>
 +
</table>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september10">
 +
<h2>10th September, Saturday</h2>
 +
<p>
 +
PCR purification of YFP digested with E was done.
 +
Digestion of purified YFP was done with S for 4 hrs at 37 degrees.
 +
<table>
 +
<tr><th></th><th>YFP</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>20</td></tr>
 +
<tr><td>Cutsmart Buffer</td><td>2.5</td></tr>
 +
<tr><td>Spel</td><td>1</td></tr>
 +
<tr><td>NF H2O</td><td>1.5</td></tr>
 +
</table>
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK NINE - ( 12th September - 13th September )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september12">
 +
<h2>12th September, Monday</h2>
 +
<p class="distance">
 +
22 ml cultures of CFP, YFP, HIV site, MASP with ATG and MASP without ATG was prepared.
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september13">
 +
<h2>13th September, Tuesday</h2>
 +
<p>
 +
Glycerol stock and plasmid extraction of  CFP, YFP, HIV site, MASP with ATG and MASP without ATG was performed.<br>
 +
CFP:437.5 ng/ul<br>
 +
YFP: 286.7 ng/ul<br>
 +
HIV Cleavage Site: 357.1 ng/ul<br>
 +
MaSP with ATG: 268.2 ng/ul<br>
 +
MaSP w/o ATG: 123ng/ul<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK TEN - ( 19th September - 24th September )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september19">
 +
<h2>19th September, Monday</h2>
 +
<p class="distance">
 +
PCR for FRET (2 sets)
 +
Digestion with E and X for HIV site and E for CFP was successfully done.<br>
 +
<table>
 +
<tr><th></th><th>CFP</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>20</td></tr>
 +
 +
<tr><td>ECoR buffer</td><td>2</td></tr>
 +
<tr><td>ECoRI</td><td>1</td></tr>
 +
<tr><td>NF H2O</td><td>14.7</td></tr>
 +
</table>
 +
 
 +
<table>
 +
<tr><th></th><th>Cleavage Site</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>2.8</td></tr>
 +
<tr><td>3.1 Buffer</td><td>2</td></tr>
 +
<tr><td>ECoRI</td><td>1</td></tr>
 +
<tr><td>Xbal</td><td>1</td></tr>
 +
<tr><td>NF H2O</td><td>13.2</td></tr>
 +
</table>
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september20">
 +
<h2>20th September, Tuesday</h2>
 +
<p>
 +
PCR purification of CFP digested with E was done.
 +
Digestion with S of purified product was done for 6 hours at 37 degrees.
 +
 
 +
<table>
 +
<tr><th></th><th>CFP</th></tr>
 +
<tr><th>Component</th><th>Volume(ul)</th></tr>
 +
<tr><td>DNA</td><td>20</td></tr>
 +
<tr><td>Cutsmart Buffer</td><td>2.5</td></tr>
 +
<tr><td>Spel</td><td>1</td></tr>
 +
<tr><td>NF H2O</td><td>1.5</td></tr>
 +
</table>
 +
 
 +
<br>
 +
Gel extraction of digested CFP and HIV site was successfully performed.
 +
The gel pieces stored at 4 degrees
 +
 
 +
<img src="pic/T--IIT_Kharagpur--20_sep.jpg" height="200px" width="200px">
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september21">
 +
<h2>21st September, Wednesday</h2>
 +
<p>
 +
Gel extraction of stored gel pieces
 +
Amount of extracted DNA too low to proceed to ligation step
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september22">
 +
<h2>22nd September, Thursday</h2>
 +
<p>
 +
S digestion of CFP and X digestion of HIV
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september23">
 +
<h2>23rd September, Friday</h2>
 +
<p>
 +
PCR purification of above digested followed by E digestion of both for 6 hrs at 37 degrees
 +
Gel extraction after completion of digestion followed by ligation of E and X digested HIV site and E and S digested CFP at 16 degrees overnight
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september24">
 +
<h2>24th September, Saturday</h2>
 +
<p>
 +
Transformed DH5 alpha cells with ligation mix and plating on A+K plates
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
<div class="container" id="content">
 +
    <h2 class="project-name">WEEK ELEVEN - ( 25th September - 2nd October )</h1>
 +
<div id="timeline">
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september25">
 +
<h2>25th September, Sunday</h2>
 +
<p class="distance">
 +
Colonies observed on incubated plates
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september26">
 +
<h2>26th September, Mondayy</h2>
 +
<p>
 +
18 colonies from the 1:3 plate were put into 1 ml media overnight for screening<br>
 +
14 colonies from the 1:5 plate were put into 1 ml media overnight for screening<br>
 +
Digestion of YFP with X and P<br>
 +
Digestion of HIV site with S and P
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september27">
 +
<h2>27th September, Tuesday</h2>
 +
<p>
 +
PCR for FRET (2 sets)<br>
 +
Gel extraction for PCR products and digestion with P<br>
 +
Digestion of K backbone with P<br>
 +
Screening for  18 colonies from the 1:3 plate and 14 colonies from the 1:5 plate<br>
 +
Colonies 1, 4, 7, 10 from 1:3 plate showed a shift<br>
 +
Colonies 1, 5, 11 from 1:5 plate showed a shift<br>
 +
 
 +
<img src="pic/T--IIT_Kharagpur--27_sep.jpg" height="200px" width="200px">
 +
Ligation of YFP digested with X and P with HIV site digested with S and P<br>
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<g>
 +
<path fill="#FFFFFF" d="M17.92,3.065l-1.669-2.302c-0.336-0.464-0.87-0.75-1.479-0.755C14.732,0.008,7.653,0,7.653,0v5.6
 +
c0,0.096-0.047,0.185-0.127,0.237c-0.081,0.052-0.181,0.06-0.268,0.02l-1.413-0.64C5.773,5.183,5.69,5.183,5.617,5.215l-1.489,0.65
 +
c-0.087,0.038-0.19,0.029-0.271-0.023c-0.079-0.052-0.13-0.141-0.13-0.235V0H2.191C1.655,0,1.233,0.434,1.233,0.97
 +
c0,0,0.025,15.952,0.031,15.993c0.084,0.509,0.379,0.962,0.811,1.242l2.334,1.528C4.671,19.905,4.974,20,5.286,20h10.307
 +
c1.452,0,2.634-1.189,2.634-2.64V4.007C18.227,3.666,18.12,3.339,17.92,3.065z M16.42,17.36c0,0.464-0.361,0.833-0.827,0.833H5.341
 +
l-1.675-1.089h10.341c0.537,0,0.953-0.44,0.953-0.979V2.039l1.459,2.027V17.36L16.42,17.36z"/>
 +
</g>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september28">
 +
<h2>28th September, Wednesday</h2>
 +
<p>
 +
PCR purification of P digested K backbone and FRET PCR followed by E digestion
 +
Gel Extraction of the digested products followed by ligation
 +
Colonies observed of ligated YFP and HIV site observed
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="september29">
 +
<h2>29th September, Thursday</h2>
 +
<p>
 +
25 colonies from the ligated YFP and HIV site plate picked for screening
 +
X and P digestion for CFP+ HIV
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
c-0.329-0.825-1.5-0.825-1.83,0L7.476,5.611c-0.132,0.346-0.462,0.578-0.824,0.61L0.894,6.766C0.035,6.848-0.312,7.921,0.333,8.499
 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
c-0.081-0.365,0.035-0.729,0.313-0.975l4.34-3.811C21.219,7.921,20.855,6.848,19.998,6.766z"/>
 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="september30">
 +
<h2>30th September, Friday</h2>
 +
<p>
 +
Screening of YFP + HIV<br>
 +
Gel run X and P digested CFP + HIV<br>
 +
20 ml cultures for plasmid extraction of K-backbone, CFP+HIV and HIV Site, YFP+HIV (colony 7) and YFP+HIV (colony 13)
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
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 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
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 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content" id="october1">
 +
<h2>1st October, Saturday</h2>
 +
<p>
 +
Plasmid extractions for the above cultures<br>
 +
Digestion with X and P for YFP+HIV (colony 7) and CFP+HIV<br>
 +
Gel run for the digested YFP+HIV (colony 7) and CFP+HIV<br>
 +
Digestion with X of YFP+HIV<br>
 +
Digestion with S of CFP
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
<div class="timeline-item">
 +
<div class="timeline-icon">
 +
<svg version="1.1" id="Layer_1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" x="0px" y="0px"
 +
width="21px" height="20px" viewBox="0 0 21 20" enable-background="new 0 0 21 20" xml:space="preserve">
 +
<path fill="#FFFFFF" d="M19.998,6.766l-5.759-0.544c-0.362-0.032-0.676-0.264-0.822-0.61l-2.064-4.999
 +
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 +
l4.338,3.811c0.279,0.246,0.395,0.609,0.314,0.975l-1.304,5.345c-0.199,0.842,0.708,1.534,1.468,1.089l4.801-2.822
 +
c0.313-0.181,0.695-0.181,1.006,0l4.803,2.822c0.759,0.445,1.666-0.23,1.468-1.089l-1.288-5.345
 +
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 +
</svg>
 +
 
 +
</div>
 +
<div class="timeline-content right" id="october2">
 +
<h2>2nd October, Sunday</h2>
 +
<p>
 +
PCR purification of the digested products<br>
 +
Digestion with E of S digested CFP and X digested YFP+HIV<br>
 +
Gel extraction of the digested product<br>
 +
Ligation of E and S digested CFP and E and X digested YFP+HIV<br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</p>
 +
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
  
 
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Revision as of 20:22, 17 October 2016

IGEM-IIT Kharagpur- Notebook

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WEEK ONE - ( 20th July - 22th July )

20th july, Wednesday

DH5 alpha competent cells were prepared for transformation experiments.

21st July, Thursday

Transformation of the prepared competent cells was carried out. The plasmids used for transformation were YFP (BBa_K592101), HIV cleavage site (BBa_I712015), and the protease construct (BBa_K743013)

22nd July, Friday

Replication plating for above transformed parts was done. This including the three sets of transformed cells containing the plasmids YFP (BBa_K592101), the HIV cleavage site (BBa_I712015), and the protease construct (BBa_K743013).

WEEK TWO - ( 25th July - 30th July )

25th july, Monday

1 litre of LB media was prepared and duly autoclaved. 5 plates each of the antibiotics chloramphenicol, ampicillin and ampicillin+Kanamycin were prepared.

26th July, Tuesday

20ml Cultures were prepared for the plasmid extraction of each of YFP (BBa_K592101), the HIV cleavage site (BBa_I712015) and the protease construct (BBa_K743013).

27th July, Wednesday

Plasmid extraction was done for YFP (BBa_K592101), the HIV cleavage site (BBa_I712015), and the protease construct (BBa_K743013) using mini-prep plasmid DNA extraction kit.

28th July, Thursday

DNA quantification was done using Nano Drop. YFP: 250 ng/ul
Protease: 170 ng/ul
Cleavage Site: 120 ng/ul
1% agarose gel was prepared for screening of the plasmids extracted by the mini prep kit.
The FRET construct was kept for overnight digestion at the restriction sites E and P.
Digestions with E and P for YFP, Protease and Cleavage Site

YFPProteaseCleavage Site
ComponentVolume(ul)Volume(ul)Volume(ul)
DNA233.5
2.1 Buffer222
ECoRI0.50.50.5
Psti0.50.5-
Nf H2O151414

WEEK THREE - ( 1st August - 7th August )

1st August, Monday

3ml cultures made for the preparation of competent cells of DH5 alpha cells. 20ml culture prepared for the construct BBa_K844004. (MaSP without ATG)

2nd August, Tuesday

11 vials of DH5 alpha competent cells were prepared. Plasmid extraction done for the construct BBa_K844004 (MaSP without ATG).

3rd August, Wednesday

As a part of the iGEM 2016 interlab study, transformations were done for the following:
Test device1
Test device2
Test device3
Positive control
Negative control
Also, transformed cells were prepared with the part BBa_K844008 (MaSP with ATG)

4th August, Thursday

Colonies were seen for all six of the transformed plates. The transformation procedure was hence successful.

7th August, Sunday

20ml Culture was prepared for the screening of BBa_K844008 (MaSP with ATG). Also, 20ml cultures were prepared for each of test device 1, test device 2, and test device 3 for the interlab study.

WEEK FOUR - ( 8th August - 12th August )

8th August, Monday

PPlasmid extraction performed for the screening of the parts BBa_K844008 (MaSP with ATG); and test device 1, test device 2, test device 3 for the interlab study.

9th August, Tuesday

Gel Electrophoresis with 1% agarose gel was done for BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3 for screening. The run was not successful due to gel over flow.

10th August, Wednesday

20ml Culture was prepared for screening of BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3.

11th August, Thursday

Plasmid extraction for the screening of BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3 was done.

12th August, Friday

Gel electrophoresis with 1% agarose gel was done for BBa_K844008 (MaSP with ATG), test device 1, test device 2, test device 3.

WEEK FIVE - ( 15th August - 18th August )

16th August, Tuesday

Plasmid extraction of BBa_K844008 (MaSP with ATG), test device 1, test device 2, and test device 3 was done with mini-prep kit. Digestion of above obtained plasmids with E And P was carried out.

17th August, Wednesday

1% agarose gel was prepared for digested plasmids PCR of protease construct for HIV site primers was done PCR parameters:
{95 degree 2 minutes
[95 degree 30 seconds
60 degrees 30 sec
68 degree 2 min] X35 cycles
68 degree 10 min}
PCR products run in 1% agarose gel.
Amplification not upto expectation.
Repetition of the experiment, with different conditions suggested.
Note: Too many non specific products were observed that is why the same was repeated next week.

18th August, Thursday

PCR of protease construct for HIV site primers was done.
PCR products were run in 1% agarose gel.
PCR Parameters:
{95 degree 2 minutes
[95 degree 20 seconds
62 degrees 30 sec
68 degree 2 min 15 sec] X35 cycles
68 degree 10 min}
Amplification not upto expectation.
Repetition of the experiment, with different conditions suggested.

WEEK SIX - ( 22nd August - 26th August )

22th August, Monday

PCR for both fret and silk constructs at two conditions was done.

Condition 1
{95 degree 5 minutes
Taq DNA polymerase is added.
[95 degree 30 seconds
57.5 degrees 40 sec
68 degree 40 sec] X35 cycles
68 degree 10 min}
Condition 2
{95 degree 2 minutes
[95 degree 30 seconds
58 degrees 1 min
68 degree 2 min] X35 cycles
68 degree 10 min}

23rd August, Tuesday

PCR for FRET construct was done. PCR Parameters:
{95 degree 2 minutes
[95 degree 30 seconds
57 degrees 40 sec
68 degree 2 min] X35 cycles
68 degree 10 min}

24th August, Wednesday

Gel run for FRET construct was performed.

25th August, Thursday

PCR for silk construct was done with the following parameters:
{95 degree 2 minutes
[95 degree 30 seconds
57 degrees 40 sec
68 degree 2 min] X35 cycles
68 degree 10 min}

26th August, Friday

PCR for FRET construct was done.
PCR Parameters:
{95 degree 2 minutes
[95 degree 30 seconds
58 degrees 1 min
68 degree 2 min] X35 cycles
68 degree 10 min}

WEEK SEVEN - ( 29th August - 2nd September )

29th August, Monday

Nano drop analysis for pSB1K3( Kanamycin backbone) was done. Result:Obtained concentration was 192.6mg/ul.

31st August, Wednesday

Digestion for Kanamycin backbone and FRET PCR with E And P

K backboneFRET PCR
ComponentVolume(ul)Volume(ul)
DNA62.3
2.1 Buffer22
ECoRI11
Psti11
Nf H2O1013.7

1st September, Thursday

Ligation for Kanamycin backbone and FRET PCR with E and P was done Vector:Insert=1:3

2nd September, Friday

Transformation for Kanamycin backbone and FRET PCR with E and P failed.
Note: Reason was determined to be inadequate amount of the PCR product

WEEK EIGHT - ( 8th September - 10th September )

8th September, Thursday

2 ml culture of CFP and YFP was prepared.
PCR done for FRET construct and silk construct
Digestion with E and P of FRET PCR product was done.

FRET PCR
ComponentVolume(ul)
DNA19
2.1 Buffer2.5
ECoRI1
PstI1
NF H2O1.5

9th September, Friday

PCR purification of digested FRET PCR product was done. Plasmid isolation of YFP and CFP was performed.
YFP: 250 ng/ul
CFP: 218 ng/ul
Glycerol stock for CFP and YFP was prepared.
Restriction digestion(overnight) of YFP and cleavage site was done.

YFP
ComponentVolume(ul)
DNA5
ECoR buffer2
ECoRI1
NF H2O12
Cleavage Site
ComponentVolume(ul)
DNA10
3.1 Buffer2
ECoRI1
Xbal1
NF H2O6

10th September, Saturday

PCR purification of YFP digested with E was done. Digestion of purified YFP was done with S for 4 hrs at 37 degrees.

YFP
ComponentVolume(ul)
DNA20
Cutsmart Buffer2.5
Spel1
NF H2O1.5

WEEK NINE - ( 12th September - 13th September )

12th September, Monday

22 ml cultures of CFP, YFP, HIV site, MASP with ATG and MASP without ATG was prepared.

13th September, Tuesday

Glycerol stock and plasmid extraction of CFP, YFP, HIV site, MASP with ATG and MASP without ATG was performed.
CFP:437.5 ng/ul
YFP: 286.7 ng/ul
HIV Cleavage Site: 357.1 ng/ul
MaSP with ATG: 268.2 ng/ul
MaSP w/o ATG: 123ng/ul

WEEK TEN - ( 19th September - 24th September )

19th September, Monday

PCR for FRET (2 sets) Digestion with E and X for HIV site and E for CFP was successfully done.

CFP
ComponentVolume(ul)
DNA20
ECoR buffer2
ECoRI1
NF H2O14.7
Cleavage Site
ComponentVolume(ul)
DNA2.8
3.1 Buffer2
ECoRI1
Xbal1
NF H2O13.2

20th September, Tuesday

PCR purification of CFP digested with E was done. Digestion with S of purified product was done for 6 hours at 37 degrees.

CFP
ComponentVolume(ul)
DNA20
Cutsmart Buffer2.5
Spel1
NF H2O1.5

Gel extraction of digested CFP and HIV site was successfully performed. The gel pieces stored at 4 degrees

21st September, Wednesday

Gel extraction of stored gel pieces Amount of extracted DNA too low to proceed to ligation step

22nd September, Thursday

S digestion of CFP and X digestion of HIV

23rd September, Friday

PCR purification of above digested followed by E digestion of both for 6 hrs at 37 degrees Gel extraction after completion of digestion followed by ligation of E and X digested HIV site and E and S digested CFP at 16 degrees overnight

24th September, Saturday

Transformed DH5 alpha cells with ligation mix and plating on A+K plates

WEEK ELEVEN - ( 25th September - 2nd October )

25th September, Sunday

Colonies observed on incubated plates

26th September, Mondayy

18 colonies from the 1:3 plate were put into 1 ml media overnight for screening
14 colonies from the 1:5 plate were put into 1 ml media overnight for screening
Digestion of YFP with X and P
Digestion of HIV site with S and P

27th September, Tuesday

PCR for FRET (2 sets)
Gel extraction for PCR products and digestion with P
Digestion of K backbone with P
Screening for 18 colonies from the 1:3 plate and 14 colonies from the 1:5 plate
Colonies 1, 4, 7, 10 from 1:3 plate showed a shift
Colonies 1, 5, 11 from 1:5 plate showed a shift
Ligation of YFP digested with X and P with HIV site digested with S and P

28th September, Wednesday

PCR purification of P digested K backbone and FRET PCR followed by E digestion Gel Extraction of the digested products followed by ligation Colonies observed of ligated YFP and HIV site observed

29th September, Thursday

25 colonies from the ligated YFP and HIV site plate picked for screening X and P digestion for CFP+ HIV

30th September, Friday

Screening of YFP + HIV
Gel run X and P digested CFP + HIV
20 ml cultures for plasmid extraction of K-backbone, CFP+HIV and HIV Site, YFP+HIV (colony 7) and YFP+HIV (colony 13)

1st October, Saturday

Plasmid extractions for the above cultures
Digestion with X and P for YFP+HIV (colony 7) and CFP+HIV
Gel run for the digested YFP+HIV (colony 7) and CFP+HIV
Digestion with X of YFP+HIV
Digestion with S of CFP

2nd October, Sunday

PCR purification of the digested products
Digestion with E of S digested CFP and X digested YFP+HIV
Gel extraction of the digested product
Ligation of E and S digested CFP and E and X digested YFP+HIV