Difference between revisions of "Team:Aix-Marseille/Notebook WIP"

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<li>Miniprep B0034</li>
 
<li>Miniprep B0034</li>
 
<li>Transformation in DH5α of interlab studies plasmids</li>
 
<li>Transformation in DH5α of interlab studies plasmids</li>
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<h5>Thursday, June 23th</h5>
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<li>Digestion E/P pSB1C3</li>
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<li>Purification pSB1C3 linéarisé purifié 23/06</li>
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<li>Digestion E/P of DesA, DesB, DesC, DesD</li>
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<li>3A Assembly of B0034 and I0500 in pSB1K3</li>
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<li>Digestion of pSB1C3 23/06</li>
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<li></li>
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Revision as of 14:50, 5 July 2016

Monday, June 13th
  • Preparation of heat competent DH5α cells
Tuesday, June 14th
  • Transformation of DH5α cells with test plasmid
Wednesday, June 15th
  • Preparation of heat competent Tg1 cells
  • Transformation of Tg1 cells with test plasmid
Thursday, June 16th
  • Transformation of DH5α cells with pSB1C3 + RFP/li>
  • Transformation with K880005, K823017, I0500, K1404006, B0034
Friday, June 17th
  • Miniprep K880005, K823017, I0500, K1404006, B0034
  • Miniprep pSB1C3 + RFP
Monday, June 20th
  • pSB1C3 digestion from 17/06 miniprep
  • pSB1C3 digestion gel migration : NO PHOTO
  • Biobricks minipreps gel migration : OK PHOTO
  • Transformation Tg1 with RFP+pSB1C3
Tuesday, June 21th
  • pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup
  • FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3
  • E. Coli and Desulfo FliC ligation in linearized pSB1C3
  • Transformation of pSB1C3 RFP in Tg1
Wednesday, June 22th
  • Transformation in DH5α of :
    • FliC coli/desulfo in pSB1C3
    • Linearized pSB1K3
    • Linearized pSB1A3
    • Linearized pSB1T3
    • Linearized pSB1C3
  • Miniprep B0034
  • Transformation in DH5α of interlab studies plasmids
Thursday, June 23th
  • Digestion E/P pSB1C3
  • Purification pSB1C3 linéarisé purifié 23/06
  • Digestion E/P of DesA, DesB, DesC, DesD
  • 3A Assembly of B0034 and I0500 in pSB1K3
  • Digestion of pSB1C3 23/06