Difference between revisions of "Team:UGent Belgium/Composite Part"

 
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<br>
 
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<div class="container">
 
<div class="container">
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Klad:
 
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<ul>
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896010" target="_blank"> BBa_K1896010 </a>: mGFPuv2 generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896011" target="_blank"> BBa_K1896011 </a>: inaZ (INP) generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896012" target="_blank"> BBa_K1896012 </a>: INP_NC-streptavidin generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896013" target="_blank"> BBa_K1896013 </a>: INP_NC-mSA2 generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896014" target="_blank"> BBa_K1896014 </a>: mGFPuv2-streptavidin generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896015" target="_blank"> BBa_K1896015 </a>: mGFPuv2-mSA2 generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896016" target="_blank"> BBa_K1896016 </a>: INPRC-mGFPuv2 generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896017" target="_blank"> BBa_K1896017 </a>: INPRC-streptavidin generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896018" target="_blank"> BBa_K1896018 </a>: INPRC-mSA2 generator
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896019" target="_blank"> BBa_K1896019 </a>: pXS-GG
 
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<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896020" target="_blank"> BBa_K1896020 </a>: pXW-GG
 
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</ul>
 
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   <h2>Composite parts</h2>
 
   <h2>Composite parts</h2>
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<div id="groupparts" style="min-height:100px;width:700px;">
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<div id="groupparts" style="min-height:100px;width:800px;">
 
   <style>
 
   <style>
 
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   <table class=" pgrouptable tablesorter" style="width: 100%;" cellpadding="0" cellspacing="0">
 
   <table class=" pgrouptable tablesorter" style="width: 100%;" cellpadding="0" cellspacing="0">
 
     <thead>
 
     <thead>
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       <tr><th style="width:8px"></th><th style="width:8px"></th><th style="width:8px"></th><th style="width: 87px;">Name</th><th style="width: 80px" colspan="">Type</th><th style="width: auto;" colspan="">Description</th><th style="width: 150px" colspan="">Designer</th><th style="width: 50px" colspan="">Length</th></tr>
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       <tr><th style="width:8px"></th><th style="width:8px"></th><th style="width:8px"></th><th style="width: 100px;">Name</th><th style="width: 67px" colspan="">Type</th><th style="width: auto;" colspan="">Description</th><th style="width: 200px" colspan="">Designer</th><th style="width: 50px" colspan="">Length</th></tr>
 
     </thead>
 
     </thead>
 
     <tbody>
 
     <tbody>
 
     <tr>
 
     <tr>
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       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896010">BBa_K1896010</a></td><td>Generator</td><td>mGFPuv2 generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">915</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896010">BBa_K1896010</a></td><td>Generator</td><td>mGFPuv2 generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">915</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896011">BBa_K1896011</a></td><td>Generator</td><td>inaZ (INP) generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">3801</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896011">BBa_K1896011</a></td><td>Generator</td><td>inaZ (INP) generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">3801</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896012">BBa_K1896012</a></td><td>Generator</td><td>INP_NC-streptavidin generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1542</td>
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       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896012">BBa_K1896012</a> &dagger;</td><td>Generator</td><td>INP_NC-streptavidin generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1542</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896013">BBa_K1896013</a></td><td>Generator</td><td>INP_NC-mSA2 generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1518</td>
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       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896013">BBa_K1896013</a></td><td>Generator</td><td>INP_NC-mSA2 generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1518</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896014">BBa_K1896014</a></td><td>Generator</td><td>mGFPuv2-streptavidin generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1332</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896014">BBa_K1896014</a> &dagger;</td><td>Generator</td><td>mGFPuv2-streptavidin generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1332</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896015">BBa_K1896015</a></td><td>Generator</td><td>mGFPuv2-mSA2 generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1308</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896015">BBa_K1896015</a></td><td>Generator</td><td>mGFPuv2-mSA2 generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">1308</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896016">BBa_K1896016</a></td><td>Generator</td><td>INPRC-mGFPuv2 generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">4023</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896016">BBa_K1896016</a></td><td>Generator</td><td>INP_RC-mGFPuv2 generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">4023</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896017">BBa_K1896017</a></td><td>Generator</td><td>INPRC-streptavidin generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">3714</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896017">BBa_K1896017</a> &dagger;</td><td>Generator</td><td>INP_RC-Streptavidin generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">3714</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896018">BBa_K1896018</a></td><td>Generator</td><td>INPRC-mSA2 generator</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">3690</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896018">BBa_K1896018</a></td><td>Generator</td><td>INP_RC-mSA2 generator</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">3690</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896019">BBa_K1896019</a></td><td>DNA</td><td>pXS-GG</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">220</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896019">BBa_K1896019</a></td><td>DNA</td><td>pXS-GG cloning construct</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">220</td>
 
     </tr>
 
     </tr>
 
     <tr>
 
     <tr>
−
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896020">BBa_K1896020</a></td><td>DNA</td><td>pXW-GG</td><td width="100px">Bob Van Hove, Maarten Van Brempt</td><td align="right">220</td>
+
       <td class="status_cell  "></td><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_white">&nbsp;&nbsp;</td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1896020">BBa_K1896020</a></td><td>DNA</td><td>pXW-GG cloning construct</td><td width="200px">Bob Van Hove, Maarten Van Brempt</td><td align="right">220</td>
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>
 
   </table>
 
   </table>
 
</div>
 
</div>
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&nbsp;<p>&dagger;: Parts encoding tetrameric streptavidin fusion proteins were found to cause growth defects and be genetically unstable.</p>
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+
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    <h3> Golden Gate cloning vectors </h3>
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−
      <h4> BBa_K18960xx: P<sub>J23119</sub>-RBS<sub>B0034</sub>-MCS<sub>BsaI</sub>-TT<sub>B0010</sub>-TT<sub>B0012</sub></h4>
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      <h4> BBa_K18960xx:  P<sub>J23105</sub>-RBS<sub>B0034</sub>-MCS<sub>BsaI</sub>-TT<sub>B0010</sub>-TT<sub>B0012</sub></h4>
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    <h3> Whole cell experiments </h3>
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      <h4> BBa_K18960xx:  inaZ generator </h4>
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      <h4> BBa_K18960xx:  INP<sub>NC</sub>-streptavidin generator </h4>
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      <h4> BBa_K18960xx:  INP<sub>NC</sub>-mSA2 generator </h4>
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−
    <h3> Lysate experiments </h3>
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      <h4> BBa_K18960xx:  mGFPuv2 generator </h4>
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      <h4> BBa_K18960xx:  mGFPuv2-streptavidin generator </h4>
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      <h4> BBa_K18960xx:  mGFPuv2-mSA2 generator </h4>
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      <h4> BBa_K18960xx:  INP<sub>RC</sub>-mGFPuv2 generator </h4>
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      <h4> BBa_K18960xx:  INP<sub>RC</sub>-streptavidin generator </h4>
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      <h4> BBa_K18960xx:  INP<sub>RC</sub>-mSA2 generator </h4>
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</div>
 
</div>
  

Latest revision as of 22:47, 17 October 2016

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Composite parts

NameTypeDescriptionDesignerLength
   BBa_K1896010GeneratormGFPuv2 generatorBob Van Hove, Maarten Van Brempt915
   BBa_K1896011GeneratorinaZ (INP) generatorBob Van Hove, Maarten Van Brempt3801
   BBa_K1896012 †GeneratorINP_NC-streptavidin generatorBob Van Hove, Maarten Van Brempt1542
   BBa_K1896013GeneratorINP_NC-mSA2 generatorBob Van Hove, Maarten Van Brempt1518
   BBa_K1896014 †GeneratormGFPuv2-streptavidin generatorBob Van Hove, Maarten Van Brempt1332
   BBa_K1896015GeneratormGFPuv2-mSA2 generatorBob Van Hove, Maarten Van Brempt1308
   BBa_K1896016GeneratorINP_RC-mGFPuv2 generatorBob Van Hove, Maarten Van Brempt4023
   BBa_K1896017 †GeneratorINP_RC-Streptavidin generatorBob Van Hove, Maarten Van Brempt3714
   BBa_K1896018GeneratorINP_RC-mSA2 generatorBob Van Hove, Maarten Van Brempt3690
   BBa_K1896019DNApXS-GG cloning constructBob Van Hove, Maarten Van Brempt220
   BBa_K1896020DNApXW-GG cloning constructBob Van Hove, Maarten Van Brempt220
 

†: Parts encoding tetrameric streptavidin fusion proteins were found to cause growth defects and be genetically unstable.