Difference between revisions of "Team:ASIJ Tokyo/Safety"

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Chassis Organism: <i> E. coli </i>, strain K-12
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We chose to use the <i> E. coli </i> strain K-12 as our chassis organism. In our experiment, we also worked with a synthesized DNA sequence found in the bacterium <i>Ideonella Sakaiensis</i> called PETase.  
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In addition to the chassis organism, ASIJ_Tokyo will be working with a synthesized DNA sequence found in Ideonella Sakaiensis called PETase.
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Goal
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Our project is to construct a usable PETase biobrick. This will be accomplished by optimizing the production and secretion of a PET degrading enzyme called PETase. Included within this construction will be an ideal promoter. We will accomplish this within an E. Coli system. We hope that this addition will allow researchers to pursue better methods of of PET degradation in the environment. 
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Risk Level
 
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E. Coli is a risk group level 1 organism, so there is relatively low risk.  To minimise risk, our group followed safety level 1 procedures. We washed hands after every experiment, disinfected lab benches, and disinfected apparatus. We also worked under biosafety cabinets. To the extent of our knowledge, the bacteria does not pose a risk to human health. Therefore, we believe that the project poses minimal to no risk if developed into a real product.  
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<i>E. coli</i>is a risk group level 1 organism, so there is relatively low risk associated with our experiment.  To minimize risk, our group followed safety level 1 procedures. We washed our hands after every experiment, disinfected lab benches, and disinfected apparatuses. We also worked under biosafety cabinets. To the extent of our knowledge, the bacteria does not pose a risk to human health. Therefore, we believe that the project poses minimal to no risk if developed into a real product.  
 
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Revision as of 02:22, 18 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Safety

Safety Information


We chose to use the E. coli strain K-12 as our chassis organism. In our experiment, we also worked with a synthesized DNA sequence found in the bacterium Ideonella Sakaiensis called PETase.
Risk Level
E. coliis a risk group level 1 organism, so there is relatively low risk associated with our experiment. To minimize risk, our group followed safety level 1 procedures. We washed our hands after every experiment, disinfected lab benches, and disinfected apparatuses. We also worked under biosafety cabinets. To the extent of our knowledge, the bacteria does not pose a risk to human health. Therefore, we believe that the project poses minimal to no risk if developed into a real product.