Difference between revisions of "Team:Korea U Seoul/Design"
| (4 intermediate revisions by 2 users not shown) | |||
| Line 2: | Line 2: | ||
<html> | <html> | ||
| − | + | <style> | |
| + | /*main colour*/ | ||
| + | .navbar-default .navbar-brand, | ||
| + | .contents-sidebar .nav>.active>a, | ||
| + | .contents-sidebar .nav>.active>a, | ||
| + | .contents-sidebar .nav>li>a:hover, | ||
| + | .contents-sidebar .nav>li>a:focus, | ||
| + | h5, | ||
| + | h2, | ||
| + | h1, | ||
| + | .main-container .section a { | ||
| + | color: #FF473A | ||
| + | ; | ||
| + | } | ||
| + | .contents-sidebar .nav>.active>a, | ||
| + | .contents-sidebar .nav>li>a:hover, | ||
| + | .contents-sidebar .nav>li>a:focus { | ||
| + | border-left: 2px solid #7F231D; | ||
| + | } | ||
| + | .image.lightbox, #notebook-key-button { | ||
| + | background-color: #FF473A; | ||
| + | } | ||
| + | /*complimentary colour*/ | ||
| + | .navbar-default .navbar-brand:hover, | ||
| + | .definition:hover, .definition:focus, | ||
| + | ol li::before, | ||
| + | .slim ul li:before, | ||
| + | .table>thead>tr>th, | ||
| + | .algorithm ol li::before, | ||
| + | .quote, | ||
| + | .quote h3, | ||
| + | a, | ||
| + | a:visited, | ||
| + | a:hover | ||
| + | { | ||
| + | color:#40120E; | ||
| + | } | ||
| + | .definition { | ||
| + | border-bottom: 1px dotted #40120E; | ||
| + | } | ||
| + | #notebook-key-button.active { | ||
| + | background-color: #40120E; | ||
| + | } | ||
| + | .popover.right>.arrow::after{ | ||
| + | border-right-color: #40120E; | ||
| + | } | ||
| + | .popover.bottom>.arrow::after { | ||
| + | border-bottom-color: #40120E; | ||
| + | } | ||
| + | .popover.left>.arrow::after { | ||
| + | border-left-color: #40120E; | ||
| + | } | ||
| + | </style> | ||
<div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2016/8/8e/KakaoTalk_20161013_202522855.jpg)"> | <div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2016/8/8e/KakaoTalk_20161013_202522855.jpg)"> | ||
<h3>Design</h3> | <h3>Design</h3> | ||
| Line 8: | Line 60: | ||
<div class="container-fluid"> | <div class="container-fluid"> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-md- | + | <div class="col-md-10 col-md-offset-1"> |
<h2>Design: Gelectricell</h2> | <h2>Design: Gelectricell</h2> | ||
<div class="section" id="design"> | <div class="section" id="design"> | ||
| Line 24: | Line 76: | ||
| − | <h4>1.Agar degradation</h4> | + | <h4>1.Agar degradation</h4><br> |
<p><font size=4>We used 3 enzymes to degrade agar in our device which are, Agarase, NABH (Neoagarobiose hydrolase), and AHGD (anhydrogalactose dehydrogenase).</p> | <p><font size=4>We used 3 enzymes to degrade agar in our device which are, Agarase, NABH (Neoagarobiose hydrolase), and AHGD (anhydrogalactose dehydrogenase).</p> | ||
| Line 30: | Line 82: | ||
<p><font size=4>Agar is first degraded into neoagarobiose by agarase. Neoagarosebiose is then degraded into D-galactose and 3.6-anhydro-L-galactose by NABH. 3.6-anhydro-L-galactose is then oxidized by NAD(P)+ dependent AHGD producing NAD(P)H.</font></p> | <p><font size=4>Agar is first degraded into neoagarobiose by agarase. Neoagarosebiose is then degraded into D-galactose and 3.6-anhydro-L-galactose by NABH. 3.6-anhydro-L-galactose is then oxidized by NAD(P)+ dependent AHGD producing NAD(P)H.</font></p> | ||
| − | <p><font size=4>All listed enzymes are displayed on the surface of E.coli BW25113 using E.coli surface display vector pATLIC.</font></p> | + | <p><font size=4>All listed enzymes are displayed on the surface of <em>E.coli</em> BW25113 using <em>E.coli</em> surface display vector pATLIC.</font></p> |
</font> | </font> | ||
| + | <br><br> | ||
| − | + | <h4>2. <em>Shewanella oneidensis</em> MR-1</h4><br> | |
| − | <h4>2. Shewanella oneidensis MR-1</h4> | + | |
<font size=4> | <font size=4> | ||
| − | <p>Shewanella oneidensis MR-1 is a bacteria that can reduce metal instead of oxygen, thus generating electricity in a battery device.</font></p> | + | <p><em>Shewanella oneidensis</em> MR-1 is a bacteria that can reduce metal instead of oxygen, thus generating electricity in a battery device.</font></p> |
| − | <p><font size=4>Shewanella oneidesis MR-1 is used in our device to generate electricity using D-galactose. However, Shewanella oneidensis MR-1 is known to be unable to use galactose as its carbon source. This is where co-cultured E.coli kicks in. E.coli BW25113 is able to utilize galactose to produce formate and acetate which Shewanella oneidensis MR-1 can utilize to generate electricity.</font></p> | + | <p><font size=4><em>Shewanella oneidesis</em> MR-1 is used in our device to generate electricity using D-galactose. However, <em>Shewanella oneidensis</em> MR-1 is known to be unable to use galactose as its carbon source. This is where co-cultured <em>E.coli</em> kicks in. <em>E.coli</em> BW25113 is able to utilize galactose to produce formate and acetate which <em>Shewanella oneidensis</em> MR-1 can utilize to generate electricity.</font></p> |
| − | </font> | + | </font><br><br> |
| − | <h4>3. Diaphorase</h4> | + | <h4>3. Diaphorase</h4><br> |
| Line 60: | Line 112: | ||
<li>Yun, Eun Ju, et al. "Production of 3, 6-anhydro-L-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40." Process biochemistry 46.1 (2011): 88-93.</li> | <li>Yun, Eun Ju, et al. "Production of 3, 6-anhydro-L-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40." Process biochemistry 46.1 (2011): 88-93.</li> | ||
<li>Yun, Eun Ju, et al. "The novel catabolic pathway of 3, 6‐anhydro‐L‐galactose, the main component of red macroalgae, in a marine bacterium." Environmental microbiology 17.5 (2015): 1677-1688.</li> | <li>Yun, Eun Ju, et al. "The novel catabolic pathway of 3, 6‐anhydro‐L‐galactose, the main component of red macroalgae, in a marine bacterium." Environmental microbiology 17.5 (2015): 1677-1688.</li> | ||
| − | <li>Ko, Hyeok-Jin, et al. "Functional cell surface display and controlled secretion of diverse agarolytic enzymes by Escherichia coli with a novel ligation-independent cloning vector based on the autotransporter YfaL." Applied and environmental microbiology 78.9 (2012): 3051-3058.</li> | + | <li>Ko, Hyeok-Jin, et al. "Functional cell surface display and controlled secretion of diverse agarolytic enzymes by <em>Escherichia coli</em> with a novel ligation-independent cloning vector based on the autotransporter YfaL." Applied and environmental microbiology 78.9 (2012): 3051-3058.</li> |
| − | <li>Wang, Victor Bochuan, et al. "Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor." Scientific reports 5 (2015).</li> | + | <li>Wang, Victor Bochuan, et al. "Metabolite-enabled mutualistic interaction between <em>Shewanella oneidensis</em> and <em>Escherichia coli</em> in a co-culture using an electrode as electron acceptor." Scientific reports 5 (2015).</li> |
<li>Zhu, Zhiguang, et al. "A high-energy-density sugar biobattery based on a synthetic enzymatic pathway." Nature communications 5 (2014).</li> | <li>Zhu, Zhiguang, et al. "A high-energy-density sugar biobattery based on a synthetic enzymatic pathway." Nature communications 5 (2014).</li> | ||
</ol> | </ol> | ||
Latest revision as of 04:09, 18 October 2016
Design
Design: Gelectricell
Our EMFC has 3 main systems.
1.Agar degradation
We used 3 enzymes to degrade agar in our device which are, Agarase, NABH (Neoagarobiose hydrolase), and AHGD (anhydrogalactose dehydrogenase).
Agar is first degraded into neoagarobiose by agarase. Neoagarosebiose is then degraded into D-galactose and 3.6-anhydro-L-galactose by NABH. 3.6-anhydro-L-galactose is then oxidized by NAD(P)+ dependent AHGD producing NAD(P)H.
All listed enzymes are displayed on the surface of E.coli BW25113 using E.coli surface display vector pATLIC.
2. Shewanella oneidensis MR-1
Shewanella oneidensis MR-1 is a bacteria that can reduce metal instead of oxygen, thus generating electricity in a battery device.
Shewanella oneidesis MR-1 is used in our device to generate electricity using D-galactose. However, Shewanella oneidensis MR-1 is known to be unable to use galactose as its carbon source. This is where co-cultured E.coli kicks in. E.coli BW25113 is able to utilize galactose to produce formate and acetate which Shewanella oneidensis MR-1 can utilize to generate electricity.
3. Diaphorase
Diaphorase is a type of enzyme that can generate electricity using NAD(P)H as the source of electron. It is expressed in BL21(DE3) with protein expression vector pB3. Diaphorase is used in our device to generate electricity using NAD(P)H produced by AHGD.
References
- Yun, Eun Ju, et al. "Production of 3, 6-anhydro-L-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40." Process biochemistry 46.1 (2011): 88-93.
- Yun, Eun Ju, et al. "The novel catabolic pathway of 3, 6‐anhydro‐L‐galactose, the main component of red macroalgae, in a marine bacterium." Environmental microbiology 17.5 (2015): 1677-1688.
- Ko, Hyeok-Jin, et al. "Functional cell surface display and controlled secretion of diverse agarolytic enzymes by Escherichia coli with a novel ligation-independent cloning vector based on the autotransporter YfaL." Applied and environmental microbiology 78.9 (2012): 3051-3058.
- Wang, Victor Bochuan, et al. "Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor." Scientific reports 5 (2015).
- Zhu, Zhiguang, et al. "A high-energy-density sugar biobattery based on a synthetic enzymatic pathway." Nature communications 5 (2014).
