Difference between revisions of "Team:ASIJ Tokyo/Parts"

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</li>
 
</li>
 
<li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest</li>
 
<li>N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest</li>
−
<li>C-Myc tag: Make our PETase construct easier to detect for Western<&nbsp>Blot to assay expression and secretion</li>
+
<li>C-Myc tag: Make our PETase construct easier to detect for Western     Blot to assay expression and secretion</li>
 
<li>PETase sequence: Needed the enzyme to degrade PET plastic</li>
 
<li>PETase sequence: Needed the enzyme to degrade PET plastic</li>
 
<li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly</li>
 
<li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly</li>

Revision as of 06:42, 18 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Parts


Andersen Promoters
  • Strength: weak (0.33) BBa_J23110
    • Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
  • Strength: moderate (0.58) BBa_J23111
    • Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
  • Strength: strong (1) BBa_J23100
    • Wanted a strong promoter to measure its PET plastic degradation up against the other promoters
  • N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest
  • C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion
  • PETase sequence: Needed the enzyme to degrade PET plastic
  • Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
  • High efficiency e. Coli cells: Were the host for our optimized PETase