Difference between revisions of "Team:IIT-Madras/Composite Part"
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= Composite Parts = | = Composite Parts = | ||
<p>We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:</p> | <p>We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:</p> | ||
| − | <p>1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from | + | <p>1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [[http://parts.igem.org/Part:BBa_K1956001 K1956001]-[http://parts.igem.org/Part:BBa_K1956004 K1956004].<br> |
| − | 2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing Gfp and Rfp proteins. Part ranges from BBa_K1956014-15, BBa_K1956017-18, BBa_K1956022-23.</p> | + | 2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing Gfp and Rfp proteins. Part ranges from [[http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[[http://parts.igem.org/Part:BBa_K1956015 15], [[http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[[http://parts.igem.org/Part:BBa_K1956018 18], [[http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[[http://parts.igem.org/Part:BBa_K1956023 23].</p> |
[[Image:Igem_dual_reporter.png | 600x100px | center]] | [[Image:Igem_dual_reporter.png | 600x100px | center]] | ||
| − | 3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005] [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality. | + | 3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality. |
[[Image:Iitm_ribos_construct.png | 600x480px | center]] | [[Image:Iitm_ribos_construct.png | 600x480px | center]] | ||
Revision as of 06:54, 18 October 2016
Composite Parts
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:
1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [[http://parts.igem.org/Part:BBa_K1956001 K1956001]-[http://parts.igem.org/Part:BBa_K1956004 K1956004].
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing Gfp and Rfp proteins. Part ranges from [[http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[[http://parts.igem.org/Part:BBa_K1956015 15], [[http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[[http://parts.igem.org/Part:BBa_K1956018 18], [[http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[[http://parts.igem.org/Part:BBa_K1956023 23].
3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.
Best Composite Part
Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.
