Difference between revisions of "Team:NAU-CHINA/Basic Part"

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<br><br>
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<h1>Best Basic Part</h1>
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<h2><a href="http://parts.igem.org/Part:BBa_K2088000">BBa_K2088000</a></h2>
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<p style="font-family:'Comic Sans MS','Arial Black';color: rgb(100,100,100);font-size:17px;">This basic part encodes Catechol 2,3-Dioxygenase from Sphingobium sp. YBL2. This protein transforms catechol by meta-cleavage to the bright yellow product 2-hydroxymuconate semialdehyde(2-HMS). We tested it with J23100 (a strong promoter) and its native rbs before it and a double terminator after it in E.coli. It plays a great role in our project to degrade catechol to 2-HMS, a non-toxic molecule that can be metalbolized by E.coli DH5α.</p>
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<p><a href="">For more details, see our experiment of C23O.</a></p>
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Revision as of 11:39, 18 October 2016



Best Basic Part

BBa_K2088000

This basic part encodes Catechol 2,3-Dioxygenase from Sphingobium sp. YBL2. This protein transforms catechol by meta-cleavage to the bright yellow product 2-hydroxymuconate semialdehyde(2-HMS). We tested it with J23100 (a strong promoter) and its native rbs before it and a double terminator after it in E.coli. It plays a great role in our project to degrade catechol to 2-HMS, a non-toxic molecule that can be metalbolized by E.coli DH5α.

For more details, see our experiment of C23O.