Difference between revisions of "Team:Paris Bettencourt/Description"

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<h2 class=project>Short Summary: What We Did</h2>
 
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<br>In this project we produced synthetic enzymes to remove red wine stains from fabric. These enzymes are designed to replace perchloroethylene (PERC), a toxic solvent used in dry cleaning that will soon be banned in France.<br>
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<br>We listened to the people who will use our product, conducting face-to-face interviews with every single dry cleaner in Paris. From this came a plan for a realistic product, a stain fighting enzymatic pretreatment compatible with existing cleaning technologies and workflows.<br>
 
  
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<br>We isolated microbes capable of degrading red wine from a library of 150 strains taken from vineyard soil samples collected around the world with the help of our fellow iGEMers. The most effective microbes were submitted for whole genome resequencing, then analyzed to produce a short list of candidate stain-fighting enzymes.<br>
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<br>We modeled enzymatic activity at the fabric surface and determined that activity could be substantially improved if the enzymes had a moderate binding affinity for the fabric itself. This effectively increases the enzyme concentration at the fabric surface and reduces the quantity of enzymes lost through diffusion into the medium.<br>
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<br>We identified short amino acid sequences with affinity to cotton, linen, wool, polyester and silk using the method of phage display. The resulting Fabric Binding Domains (FBDs) were quantitiatively characterized using ELISA, to determine peptides with optimal affinity as determined by our model.<br>
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<h2>Achievements</h2>
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<br>We developed a high-throughput assay to quantify stain removal on real cloth. Laser-cut fabric samples are sealed to 96-well microplates and imaged on flat-bed scanners. We coded custom image analysis software to identify each circular fabric sample and measure the stain intensity.<br>
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<br>We constructed new BioBricks, fusions of our most promising fabric binding domains to our favorite wine-degrading enzymes. The resulting proteins were expressed, purified and characterized both in vitro (in solution) and in situ (on real stained fabric).<br>
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Results
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<br>Finally, we succeeded in increasing the degradation of red wine stains by XX%. These results were obtained under real world conditions, on store-bought fabrics stained with our favorite red wines. We hope these pages convince you that our project is mature, thoroughly documented and effective.<br>
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  <a href="https://2016.igem.org/Team:Paris_Bettencourt/Medal_Requirements" title="Medal Requirements">
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Medal Requirements
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Sources:<br>
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<a href="https://pubchem.ncbi.nlm.nih.gov/compound/tetrachloroethylene#section=Other-Preventative-Measures">Perchloroethylene, Pubchem</a> <br>
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<a href="https://toxtown.nlm.nih.gov/text_version/chemicals.php?id=22">US National Library of Medecine </a> <br>
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  <a href="http://ntp.niehs.nih.gov/ntp/roc/content/listed_substances_508.pdf">Report on Carcinogens, Thirteenth edition</a> <br>
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Interlab Study
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  <a href="http://www.developpement-durable.gouv.fr/Le-perchloroethylene-interdit-dans.html">Ministère de l'Environnement, de l'Énergie, et de la Mer</a> <br>
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Revision as of 12:39, 18 October 2016


Achievements

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam purus nisi, aliquam sit amet tortor eget, vulputate laoreet urna. Sed sodales ex cursus enim pulvinar viverra. Nulla a erat congue, sagittis nisl eget, auctor purus. Nulla facilisi. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Aliquam consectetur sagittis accumsan. Praesent consequat, magna id aliquet tincidunt, orci ipsum consectetur risus, eu viverra lorem purus vitae purus. Nunc sagittis, nisi in vestibulum auctor, lorem sapien fermentum tellus, vitae dictum risus orci in turpis. Aliquam ac orci ut risus interdum fringilla sed eget ante. Nulla lacus est, mattis non placerat vitae, tristique at purus. Mauris ullamcorper ut quam in tincidunt. Nulla facilisi. Proin luctus non velit ac fringilla.

Etiam tincidunt lacus a commodo fermentum. Duis pretium leo nec ligula facilisis pulvinar. Nunc semper mi ac tortor ultrices commodo. Praesent vel dolor magna. Fusce ac maximus mi. Fusce blandit, felis placerat vulputate iaculis, elit nisi vulputate eros, commodo malesuada velit odio et lacus. Sed faucibus, metus ac facilisis scelerisque, risus risus ultricies massa, nec elementum eros enim et purus. Fusce vitae nunc nunc.

Ut cursus interdum sapien, in elementum augue. Donec non turpis ac ante eleifend pretium vel at dolor. Nam nec ipsum nec libero dapibus tincidunt eget nec quam. Fusce ac est vel tellus consequat tristique at nec elit. Duis in urna tincidunt eros consectetur luctus. Praesent laoreet placerat augue. Curabitur eu sapien dapibus, convallis est sit amet, pharetra lacus. Curabitur non pellentesque arcu, a viverra erat. Ut scelerisque justo non ligula dictum semper. Donec posuere tellus orci, non vestibulum massa condimentum in. Donec nibh urna, vulputate et lacus viverra, placerat convallis nulla. Quisque mi leo, hendrerit a lacinia nec, dignissim non lorem.

Morbi vitae arcu faucibus, scelerisque sem at, congue massa. Sed fringilla pharetra metus vehicula ultricies. Nunc egestas volutpat erat a congue. Pellentesque suscipit vehicula erat, sit amet semper dolor imperdiet quis. Cras vitae odio tincidunt, tincidunt elit eget, pharetra ex. Nullam in neque tincidunt, faucibus mi quis, accumsan est. Cras nibh nulla, lacinia at mauris ut, scelerisque bibendum quam. Curabitur interdum efficitur dolor, sit amet commodo diam dapibus ac. Vestibulum facilisis dolor ut erat lobortis vestibulum. Praesent vestibulum, est nec scelerisque fermentum, lectus dui ullamcorper sapien, quis pulvinar diam nibh non diam. Quisque sollicitudin turpis vel sem ullamcorper, ac aliquet elit aliquam. Proin pellentesque bibendum ornare.

Vivamus nec consectetur mi. Maecenas placerat tortor non justo hendrerit tempus quis vitae quam. Quisque egestas vel nisi vel maximus. Etiam egestas nulla diam, a ornare nisl congue ac. Sed quis dui tincidunt, fermentum sem in, facilisis dui. Vestibulum bibendum consequat erat, a facilisis velit condimentum vitae. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Duis at metus sem. Cras accumsan libero ut neque pulvinar pretium. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur egestas dui et euismod ultricies. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas.

Results
Medal Requirements
Parts
Interlab Study
Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
igem2016parisbettencourt@gmail.com
2016.igem.org