Difference between revisions of "Team:Northwestern/06 20"

 
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/06_19"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
 
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Latest revision as of 13:37, 18 October 2016

Notebook

Monday, June 20th

Agenda:

  • Make TSS Buffer
  • Make Competent Cells
  • Transform parts out of the iGEM registry

Tasks:

Jordan, Paul, & Shu

  • Made TSS
    • 5g of PEG 8000
    • 2.5mL of DMSO
    • 1.5mL of 1M MgCl
  • Filtered LB
    • Used 10mL syringe filter to sterilize
    • Stored on ice
  • Made QuickCompetent Cells
    • Diluted 5 tubes of cells up to 5mL in culture tubes at 10:30am
    • Removed at 2:20 at OD600=0.37
    • Resuspended practice plasmid DNA in 100mL of dH2O, vortexed, spun down
    • Transformed cells with 5μL of resuspended DNA
  • Followed iGEM transformation procedures
    • Incubated for 1hr 45min