Difference between revisions of "Team:Northwestern/06 24"

 
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     <h1>Friday, June 24<sup>th</sup></h3>
 
     <h1>Friday, June 24<sup>th</sup></h3>
 
     <h2>Agenda:</h2>
 
     <h2>Agenda:</h2>
−
 
+
<ul>
 +
      <li>Split Cas9 construct and research</li>
 +
      <li>Review experiment presentation</li>
 +
      <li>Research questions</li>
 +
      <li>Safety sheets</li>
 +
      <li>Interlab</li>
 +
      <li>Edit GenScript grant</li>
 +
      <li>Integrated human practices</li>
 +
      <li>Website</li>
 +
      <li>iGEM kit DNA concentration?</li>
 +
      <li>Go through kit, look for enzymes</li>
 +
    </ul>
 +
<h2>Tasks:</h2>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
         
 +
          <li>Searched for rapamycin-inducible dimer Cas9</li>
 +
          <li>Helped with some of the Gibson Cas9 constructs</li>
 +
          <li>Watched Genome Compiler tutorials </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Paul</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked on presentation</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked on GenScript</li>
 +
          <li>Helped Jordan with iGEM Interlab data colllection</li>
 +
          <li>Helped Sara make Cas9 split parts and modified Tat and Sec parts on Genome Compiler</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
         
 +
          <li>Searched the iGEM 2016 plates for practice material</li>
 +
          <li>Website CSS reading</li>
 +
          <li>Research on OMV formation and uptake for Patrick</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Revised presentation/experimental procedure</li>
 +
          <li>Redesigned proteins fusions</li>
 +
          <li>Ran Gibson on split Cas9, generated usable primers</li>
 +
          <li>Met with Promega, emailed them needed products</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Shu</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Sorted out the stuff in the freezer</li>
 +
          <li>Researched on potential membrane proteins</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Reviewed the presentation and prepared to present</li>
 +
          <li>Presented to Jewett</li>
 +
          <li>Wrote to Jennifer</li>
 +
          <li>Got worried about the DNA concentrations and whether we need to PCR more DNA, and as a result, whether we need to design a bunch of primers ASAP</li>
 +
          <li>Helped edit GenScript</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Jordan</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Sampled the <a href="https://drive.google.com/file/d/0B9EAXnPGprpBU3NNdzdRenRJSk0/view">Interlab</a> cell culture every hour for six hours</li>
 +
          <li>Interlab almost done!</li>
 +
          <li>Looked a little bit at the GenScript app</li>
 +
          <li>Watched some Genome Compiler tutorials</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
      </article>
 
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/06_23"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
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+
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/06_27">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
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Latest revision as of 13:38, 18 October 2016

Notebook

Friday, June 24th

Agenda:

  • Split Cas9 construct and research
  • Review experiment presentation
  • Research questions
  • Safety sheets
  • Interlab
  • Edit GenScript grant
  • Integrated human practices
  • Website
  • iGEM kit DNA concentration?
  • Go through kit, look for enzymes

Tasks:

Sara

  • Searched for rapamycin-inducible dimer Cas9
  • Helped with some of the Gibson Cas9 constructs
  • Watched Genome Compiler tutorials

Paul

  • Worked on presentation

Tasfia

  • Worked on GenScript
  • Helped Jordan with iGEM Interlab data colllection
  • Helped Sara make Cas9 split parts and modified Tat and Sec parts on Genome Compiler

Michelle

  • Searched the iGEM 2016 plates for practice material
  • Website CSS reading
  • Research on OMV formation and uptake for Patrick

Tyler

  • Revised presentation/experimental procedure
  • Redesigned proteins fusions
  • Ran Gibson on split Cas9, generated usable primers
  • Met with Promega, emailed them needed products

Shu

  • Sorted out the stuff in the freezer
  • Researched on potential membrane proteins

Sam

  • Reviewed the presentation and prepared to present
  • Presented to Jewett
  • Wrote to Jennifer
  • Got worried about the DNA concentrations and whether we need to PCR more DNA, and as a result, whether we need to design a bunch of primers ASAP
  • Helped edit GenScript

Jordan

  • Sampled the Interlab cell culture every hour for six hours
  • Interlab almost done!
  • Looked a little bit at the GenScript app
  • Watched some Genome Compiler tutorials