Difference between revisions of "Team:NYMU-Taipei/Demonstrate"

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<h2 style="margin-top:20px; margin-bottom:10px;">Overview</h2><hr />
 
<h2 style="margin-top:20px; margin-bottom:10px;">Overview</h2><hr />
  
<p style="font-size:16px;">Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap. </p>
+
<p style="font-size:16px;">Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.</p>
  
 
<p style="font-size:16px;">In modern bait traps, the bait only attract B. dorsalis. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.</p>
 
<p style="font-size:16px;">In modern bait traps, the bait only attract B. dorsalis. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.</p>
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<p style="font-size:16px;">We present the Integrated Orchard Safeguard, a informative defense system that links our <font color="#FF0000"><b>bait trap, IR counter, and smartphone app.</b></font></p>
 
<p style="font-size:16px;">We present the Integrated Orchard Safeguard, a informative defense system that links our <font color="#FF0000"><b>bait trap, IR counter, and smartphone app.</b></font></p>
  
<div align="center">
+
<img src="https://static.igem.org/mediawiki/2016/a/a1/T--NYMU-Taipei--photo-OFF-related-14632532_120300000685765068_1524025076_o.png" width="100%" />
<img src="https://static.igem.org/mediawiki/2016/a/a1/T--NYMU-Taipei--photo-OFF-related-14632532_120300000685765068_1524025076_o.png" width="80%" /></div>
+
  
 
</div>
 
</div>
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<p style="font-size:16px;">After the bait trap receives the population size of oriental fruit fly from the IR counter, it will then <font color="#FF0000"><b>automatically regulate the frequency </b></font>of the spray of fungal spore solution and the opening duration of the door.</p>
 
<p style="font-size:16px;">After the bait trap receives the population size of oriental fruit fly from the IR counter, it will then <font color="#FF0000"><b>automatically regulate the frequency </b></font>of the spray of fungal spore solution and the opening duration of the door.</p>
  
<div class="imageimage">
 
 
<img src="https://static.igem.org/mediawiki/2016/9/91/T--NYMU-Taipei--photo-OFF-related-14628131_120300000688268755_160847704_n.jpg" width="100%" />
 
<img src="https://static.igem.org/mediawiki/2016/9/91/T--NYMU-Taipei--photo-OFF-related-14628131_120300000688268755_160847704_n.jpg" width="100%" />
</div>
+
 
 
</div>
 
</div>
  
 
<div class="fund">
 
<div class="fund">
 +
 
<h3 style="margin-top:30px; margin-bottom:10px;">Design</h3><br />
 
<h3 style="margin-top:30px; margin-bottom:10px;">Design</h3><br />
 
<img src="https://static.igem.org/mediawiki/2016/a/a7/T-NYMU-Taipei-photo-trap_1.png" width="100%" />
 
<img src="https://static.igem.org/mediawiki/2016/a/a7/T-NYMU-Taipei-photo-trap_1.png" width="100%" />
  
<h4 style="margin-top:30px; margin-bottom:10px;">1. The First Layer – Microcontroller, Power</h4><br />
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">1. The First Layer – Microcontroller, Power</h4><br />
  
<div class="imageimage">
+
<img src="https://static.igem.org/mediawiki/2016/6/6f/T-NYMU-Taipei-photo-real_floor.png" width="90%" />
<div style="position:relative; width:48%;">
+
 
<img src="https://static.igem.org/mediawiki/2016/6/6f/T-NYMU-Taipei-photo-real_floor.png" width="100%" /></div>
+
<img src="https://static.igem.org/mediawiki/2016/f/f3/T--NYMU-Taipei--photo-OFF-related-14725282_120300000683641886_996432644_o.png" width="90%" />
<div style="position:relative; width:48%;">
+
<img src="https://static.igem.org/mediawiki/2016/f/f3/T--NYMU-Taipei--photo-OFF-related-14725282_120300000683641886_996432644_o.png" width="100%" /></div>
+
</div>
+
  
 
<p style="font-size:16px;">The Arduino UNO in our design has combined with Appsduino's product, AppsBee Shield and Appsduino Shield. (The web site of Appsduino: http://appsduino.com/)</p>
 
<p style="font-size:16px;">The Arduino UNO in our design has combined with Appsduino's product, AppsBee Shield and Appsduino Shield. (The web site of Appsduino: http://appsduino.com/)</p>
  
<h4 style="margin-top:30px; margin-bottom:10px;">2. The Second Layer – Motor</h4><br />
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">2. The Second Layer – Motor</h4><br />
  
<div class="imageimage">
+
<img src="https://static.igem.org/mediawiki/2016/c/c7/T-NYMU-Taipei-photo-roof.png" width="90%" />
<div style="position:relative; width:48%;">
+
<img src="https://static.igem.org/mediawiki/2016/c/c7/T-NYMU-Taipei-photo-roof.png" width="100%" /></div>
+
<div style="position:relative; width:48%;">
+
<p style="font-size:16px;">We use the string attached to a ULN2003 motor to open and close the door.</p></div>
+
</div>
+
  
<h4 style="margin-top:30px; margin-bottom:10px;">3. Drawer – Entrance, Atomization Device</h4><br />
+
<p style="font-size:16px;">We use the string attached to a ULN2003 motor to open and close the door.</p>
  
<div class="imageimage">
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">3. Drawer – Entrance, Atomization Device</h4><br />
<div style="position:relative; width:48%;">
+
 
<img src="https://static.igem.org/mediawiki/2016/a/a9/T-NYMU-Taipei-photo-floor_1.png" width="100%" /></div>
+
<img src="https://static.igem.org/mediawiki/2016/a/a9/T-NYMU-Taipei-photo-floor_1.png" width="100%" />
<div style="position:relative; width:48%;">
+
 
<p style="font-size:16px;">Since flying up is one of the behaviors of B. dorsalis, we set the <font color="#FF0000"><b>entrance</b></font> at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the <font color="#FF0000"><b>methyl eugenol(a pheromone precursor)</b></font> to maintain the trap's attraction to male flies. The <font color="#FF0000"><b>atomization device</b></font> in the drawer sprays fungal spore solution to infect the trapped male flies.</p></div>
+
<p style="font-size:16px;">Since flying up is one of the behaviors of B. dorsalis, we set the <font color="#FF0000"><b>entrance</b></font> at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the <font color="#FF0000"><b>methyl eugenol(a pheromone precursor)</b></font> to maintain the trap's attraction to male flies. The <font color="#FF0000"><b>atomization device</b></font> in the drawer sprays fungal spore solution to infect the trapped male flies.</p>
</div>
+
  
 
</div>
 
</div>
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<h3 style="margin-top:30px; margin-bottom:10px;">Features</h3>
 
<h3 style="margin-top:30px; margin-bottom:10px;">Features</h3>
  
<h4 style="margin-top:30px; margin-bottom:10px;">1. Specificity - Methyl Eugenol</h4>
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">1. Specificity - Methyl Eugenol</h4>
<p style="font-size:16px;">
+
<p style="font-size:16px;"><font color="#FF0000"><b>Methyl Eugenol</b></font> is the <font color="#FF0000"><b>pheromone precursor</b></font> of B. dorsalis. It only attracts male flies. The male flies will fly away once they  have consumed enough Methyl Eugenol.</p>
<font color="#FF0000"><b>Methyl Eugenol</b></font> is the <font color="#FF0000"><b>pheromone precursor</b></font> of B. dorsalis. It only attracts male flies. The male flies will fly away once they  have consumed enough Methyl Eugenol.
+
 
</p>
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">2. Target main Problem – Female B. dorsalis</h4>
<h4 style="margin-top:30px; margin-bottom:10px;">2. Target main Problem – Female B. dorsalis</h4>
+
<p style="font-size:16px;"><font color="#FF0000"><b>female B. dorsalis</b></font> poses more threat than males. </p>
<p style="font-size:16px;">
+
 
<font color="#FF0000"><b>female B. dorsalis</b></font> poses more threat than males.  
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">3. Regulation – IR Counter, Trap, Phone App</h4>
</p>
+
<p style="font-size:16px;"><font color="#FF0000"><b>Automatically regulation</b></font> of the spray frequency and door open duration depends on the data collected by the IR counter and phone APP.</p>
<h4 style="margin-top:30px; margin-bottom:10px;">3. Regulation – IR Counter, Trap, Phone App</h4>
+
 
<p style="font-size:16px;">
+
<h4 style="margin-top:30px; margin-bottom:10px; line-height: 24px;">4. Eco-Friendly – Recycle </h4>
<font color="#FF0000"><b>Automatically regulation</b></font> of the spray frequency and door open duration depends on the data collected by the IR counter and phone APP.
+
<p style="font-size:16px;">Replacing the indissoluble plastic material (distributed by the government) with <font color="#FF0000"><b>recyclable wood chips</b></font>, which can reduce environmental pollution.</p>
</p>
+
<h4 style="margin-top:30px; margin-bottom:10px;">4. Eco-Friendly – Recycle </h4>
+
<p style="font-size:16px;">
+
Replacing the indissoluble plastic material (distributed by the government) with <font color="#FF0000"><b>recyclable wood chips</b></font>, which can reduce environmental pollution.
+
</p>
+
  
 
</div>
 
</div>
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<div class="fund" align="center">
 
<div class="fund" align="center">
 
<h3 style="margin-top:30px; margin-bottom:10px;">Functionality Display Video</h3><br />
 
<h3 style="margin-top:30px; margin-bottom:10px;">Functionality Display Video</h3><br />
<iframe width="560" height="315" src="https://www.youtube.com/embed/-1yPsO4_5a4" frameborder="0" allowfullscreen ></iframe>
+
<iframe width="100%" src="https://www.youtube.com/embed/-1yPsO4_5a4" frameborder="0" allowfullscreen ></iframe>
 
</div>
 
</div>
  
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</p>
 
</p>
  
<div class="imageimage">
+
<img src="https://static.igem.org/mediawiki/2016/6/69/T-NYMU-Taipei-photo-counter_in_field.jpg" width="100%" />
<img src="https://static.igem.org/mediawiki/2016/6/69/T-NYMU-Taipei-photo-counter_in_field.jpg" width="100%" /></div>
+
  
 
</div>
 
</div>
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<div class="fund" align="center">
 
<div class="fund" align="center">
 
<h3 style="margin-top:30px; margin-bottom:10px; height:20px;">Design</h3><br />
 
<h3 style="margin-top:30px; margin-bottom:10px; height:20px;">Design</h3><br />
 
 
<img src="https://static.igem.org/mediawiki/2016/f/fe/T-NYMU-Taipei-photo-14741853_120300000623455056_66387865_n.jpg" width="80%" />
 
<img src="https://static.igem.org/mediawiki/2016/f/fe/T-NYMU-Taipei-photo-14741853_120300000623455056_66387865_n.jpg" width="80%" />
 
</div>
 
</div>
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<h3 style="margin-top:30px; margin-bottom:10px;">Functional Test</h3>
 
<h3 style="margin-top:30px; margin-bottom:10px;">Functional Test</h3>
  
<div class="imageimage">
+
<img src="https://static.igem.org/mediawiki/2016/thumb/4/4d/T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg/180px-T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg" width="90%" />
  
<div style="position:relative; width:48%;">
+
<p style="font-size:16px;">To <font color="#FF0000"><b>improve the accuracy</b></font> of counter, we tried to find the proper tunnel diameter allows only <font color="#FF0000"><b>one-by-one passage of B. dorsalis</b></font>. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol. </p>
<img src="https://static.igem.org/mediawiki/2016/thumb/4/4d/T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg/180px-T-NYMU-Taipei-photo-14647197_120300000625973835_1048154200_o.jpg" width="100%" /></div>
+
 
+
<div style="position:relative; width:48%;">
+
<p style="font-size:16px;">To <font color="#FF0000"><b>improve the accuracy</b></font> of counter, we tried to find the proper tunnel diameter allows only <font color="#FF0000"><b>one-by-one passage of B. dorsalis</b></font>. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol. </p></div>
+
 
+
</div>
+
  
 
</div>
 
</div>
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<p style="font-size:16px;">Results indicate that when the tunnel diameter is above <font color="#FF0000"><b>0.8 cm</b></font>, the flies can pass through.</p>
 
<p style="font-size:16px;">Results indicate that when the tunnel diameter is above <font color="#FF0000"><b>0.8 cm</b></font>, the flies can pass through.</p>
  
<table border="1" class="tablesatisfy">
+
<div class="tablesatisfysp" style="overflow-x:auto;">
 +
<table border="1">
 
 <tr>
 
 <tr>
 
     <td>Diameter(cm)</td>
 
     <td>Diameter(cm)</td>
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 </tr>
 
 </tr>
 
</table>
 
</table>
 +
</div>
  
 
</div>
 
</div>
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<h2 style="margin-top:30px; margin-bottom:10px;">Modeling Efficiency</h2><hr>
 
<h2 style="margin-top:30px; margin-bottom:10px;">Modeling Efficiency</h2><hr>
 +
 
<p style="font-size:16px;">
 
<p style="font-size:16px;">
 
A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
 
A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
 
<br>
 
<br>
 
<a href="https://2016.igem.org/Team:NYMU-Taipei/Project-Model">Link to modeling</a>
 
<a href="https://2016.igem.org/Team:NYMU-Taipei/Project-Model">Link to modeling</a>
 
 
</p>
 
</p>
 +
</div>
  
<h3 style="margin-top:30px; margin-bottom:10px;">Population size without IOS</h3><hr>
+
<div class="fund">
<video src="https://static.igem.org/mediawiki/2016/b/bc/T-NYMU-Taipei-model-movie.mp4"
+
 
controls
+
<h3 style="margin-top:30px; margin-bottom:10px;">Population size without IOS</h3><br />
autoplay
+
<video src="https://static.igem.org/mediawiki/2016/b/bc/T-NYMU-Taipei-model-movie.mp4" controls autoplay width="100%">
width="100%">
+
 
</video><br>
 
</video><br>
<h3 style="margin-top:30px; margin-bottom:10px;">Population size with IOS</h3><hr>
+
<h3 style="margin-top:30px; margin-bottom:10px;">Population size with IOS</h3><br />
<video src="https://static.igem.org/mediawiki/2016/d/d4/T-NYMU-Taipei-photo-model1mb.mp4"
+
<video src="https://static.igem.org/mediawiki/2016/d/d4/T-NYMU-Taipei-photo-model1mb.mp4" controls autoplay width="100%">
controls
+
autoplay
+
width="100%">
+
 
</video><br>
 
</video><br>
 +
</div>
  
 +
<div class="fund">
  
 
<h3 style="margin-top:30px; margin-bottom:10px;">Expenditure Analysis</h3><hr>
 
<h3 style="margin-top:30px; margin-bottom:10px;">Expenditure Analysis</h3><hr>
<p style="font-size:16px;">
 
Based on our experimental deployment of counters and traps in an orchard one-hectare in size. The estimated total cost of IOS is listed below:
 
</p>
 
  
 +
<p style="font-size:16px;">Based on our experimental deployment of counters and traps in an orchard one-hectare in size. The estimated total cost of IOS is listed below:</p>
 +
 +
<div class="tablesatisfysp" style="overflow-x:auto;">
 
<table cellspacing="0" cellpadding="0" border="1">
 
<table cellspacing="0" cellpadding="0" border="1">
 
     <tbody>
 
     <tbody>
 
         <tr>
 
         <tr>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;"><strong> </strong></p>
                    <strong> </strong>
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;"><strong>amount</strong></p>
                    <strong>amount</strong>
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="231" valign="top">
 
             <td width="231" valign="top">
                 <p>
+
                 <p style="font-size:16px;"><strong>cost (USD)</strong></p>
                    <strong>cost (USD)</strong>
+
                </p>
+
 
             </td>
 
             </td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;"><strong>trap</strong></p>
                    <strong>trap</strong>
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;">4</p>
                    4
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="231" valign="top">
 
             <td width="231" valign="top">
                 <p>
+
                 <p style="font-size:16px;">60</p>
                    60
+
                </p>
+
 
             </td>
 
             </td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;"><strong>counter</strong></p>
                    <strong>counter</strong>
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;">3</p>
                    3
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="231" valign="top">
 
             <td width="231" valign="top">
                 <p>
+
                 <p style="font-size:16px;">30</p>
                    30
+
                </p>
+
 
             </td>
 
             </td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;"><strong>methyl eugenol</strong></p>
                    <strong>methyl eugenol</strong>
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="230" valign="top">
 
             <td width="230" valign="top">
                 <p>
+
                 <p style="font-size:16px;">100 mL</p>
                    100 mL
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="231" valign="top">
 
             <td width="231" valign="top">
                 <p>
+
                 <p style="font-size:16px;"> 50</p>
                    50
+
                </p>
+
 
             </td>
 
             </td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
 
             <td colspan="2" width="461" valign="top">
 
             <td colspan="2" width="461" valign="top">
                 <p align="center">
+
                 <p align="center" style="font-size:16px;"><strong>total cost</strong></p>
                    <strong>total cost</strong>
+
                </p>
+
 
             </td>
 
             </td>
 
             <td width="231" valign="top">
 
             <td width="231" valign="top">
                 <p>
+
                 <p style="font-size:16px;">90</p>
                    90
+
                </p>
+
 
             </td>
 
             </td>
 
         </tr>
 
         </tr>
 
     </tbody>
 
     </tbody>
 
</table>
 
</table>
<p style="font-size:16px;">
+
</div>
It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.
+
</p>
+
  
<img src="https://static.igem.org/mediawiki/2016/d/d3/T-NYMU-Taipei-photo-protype_image005.png" width="100%" />
+
</div>
  
<p style="font-size:16px;">(cost of IOS only in low ratio compared to the total profit)</p>
+
<div class="fund">
  
<img src="https://static.igem.org/mediawiki/2016/0/00/T-NYMU-Taipei-photo-protype_image007.png" width="100%" />
+
<p style="font-size:16px;">It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.</p>
  
<p style="font-size:16px;">(figure showing the great savings from IOS)</p>
+
<div align="center">
 +
<img src="https://static.igem.org/mediawiki/2016/d/d3/T-NYMU-Taipei-photo-protype_image005.png" width="100%" /></div>
  
<h3 style="margin-top:30px; margin-bottom:10px;">Reference:</h3><hr>
+
<p style="font-size:16px;">(cost of IOS only in low ratio compared to the total profit)</p><br />
 +
 
 +
<div align="center">
 +
<img src="https://static.igem.org/mediawiki/2016/0/00/T-NYMU-Taipei-photo-protype_image007.png" width="100%" /></div>
 +
 
 +
<p style="font-size:16px;">(figure showing the great savings from IOS)</p><br />
 +
 
 +
<h3 style="margin-top:30px; margin-bottom:10px;">Reference:</h3><hr />
  
 
<p style="font-size:16px;">
 
<p style="font-size:16px;">
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Revision as of 15:01, 18 October 2016

Prototype

Prototype



Integrated Orchard Safeguard

Overview


Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.

In modern bait traps, the bait only attract B. dorsalis. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.

We present the Integrated Orchard Safeguard, a informative defense system that links our bait trap, IR counter, and smartphone app.

Bait Trap


After the bait trap receives the population size of oriental fruit fly from the IR counter, it will then automatically regulate the frequency of the spray of fungal spore solution and the opening duration of the door.

Design


1. The First Layer – Microcontroller, Power


The Arduino UNO in our design has combined with Appsduino's product, AppsBee Shield and Appsduino Shield. (The web site of Appsduino: http://appsduino.com/)

2. The Second Layer – Motor


We use the string attached to a ULN2003 motor to open and close the door.

3. Drawer – Entrance, Atomization Device


Since flying up is one of the behaviors of B. dorsalis, we set the entrance at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the methyl eugenol(a pheromone precursor) to maintain the trap's attraction to male flies. The atomization device in the drawer sprays fungal spore solution to infect the trapped male flies.


Features

1. Specificity - Methyl Eugenol

Methyl Eugenol is the pheromone precursor of B. dorsalis. It only attracts male flies. The male flies will fly away once they have consumed enough Methyl Eugenol.

2. Target main Problem – Female B. dorsalis

female B. dorsalis poses more threat than males.

3. Regulation – IR Counter, Trap, Phone App

Automatically regulation of the spray frequency and door open duration depends on the data collected by the IR counter and phone APP.

4. Eco-Friendly – Recycle

Replacing the indissoluble plastic material (distributed by the government) with recyclable wood chips, which can reduce environmental pollution.

Mechanism


Functionality Display Video


IR Counter & APP


IR counter records and provides real-time information of the orchard : pest group size, meteorological data including temperature and humidity. With a smartphone app, the bait traps are able to inform the grower of these important information.

Design


Functional Test

To improve the accuracy of counter, we tried to find the proper tunnel diameter allows only one-by-one passage of B. dorsalis. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol.

Result

Results indicate that when the tunnel diameter is above 0.8 cm, the flies can pass through.

                                       
Diameter(cm)2.001.751.501.000.800.750.60
outcomeOOOOOHardlyX

Modeling Efficiency


A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
Link to modeling

Population size without IOS



Population size with IOS



Expenditure Analysis


Based on our experimental deployment of counters and traps in an orchard one-hectare in size. The estimated total cost of IOS is listed below:

amount

cost (USD)

trap

4

60

counter

3

30

methyl eugenol

100 mL

50

total cost

90

It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.

(cost of IOS only in low ratio compared to the total profit)

(figure showing the great savings from IOS)

Reference:


1.http://www.mdais.gov.tw/files/web_articles_files/mdares/2469/631.pdf 2.http://ir.tari.gov.tw:8080/bitstream/345210000/281/1/57_1_5.pdf

Integrated Orchard Safeguard

Overview


Current method of applying entomogenous fungal pesticides is to suspend fungal spores into a solution and introduced through large-scaled spraying. However, with genetically engineered fungi, the contemporary biopesticide deployment method risk damaging the eco-system from the residual fungi. Our project is divided into two main focuses. While our team members in wet lab aim to design a killing switch circuit to decrease the environmental risk of genetically engineered fungus from within the organism. Our prototype team try to avoid the risk of large-scaled pesticide spraying by designing and building an Integrated Orchard Safeguard system centered around our prototype bait trap.

In modern bait traps, the bait only attract B. dorsalis. However, it is female flies that is responsible for the species' high reproductiveness and destructiveness. This is why we designed the bait trap to attract, infect, and release male flies to allow M. anisopliae infection of female flies via copulation. The IR counter, another feature of our prototype, record and provide information including pest population size and meteorological data, such as temperature and humidity. Furthermore, farmers can monitor their orchards with a smartphone app that receives data from the IR counter.

We present the Integrated Orchard Safeguard, a informative defense system that links our bait trap, IR counter, and smartphone app.

Bait Trap


After the bait trap receives the population size of oriental fruit fly from the IR counter, it will then automatically regulate the frequency of the spray of fungal spore solution and the opening duration of the door.

Design


1. The First Layer – Microcontroller, Power


The Arduino UNO in our design has combined with Appsduino's product, AppsBee Shield and Appsduino Shield. (The web site of Appsduino: http://appsduino.com/)

2. The Second Layer – Motor


We use the string attached to a ULN2003 motor to open and close the door.

3. Drawer – Entrance, Atomization Device


Since flying up is one of the behaviors of B. dorsalis, we set the entrance at the bottom of the trap to hinder the escape of trapped flies. The grower can pull out the drawer to refill the methyl eugenol(a pheromone precursor) to maintain the trap's attraction to male flies. The atomization device in the drawer sprays fungal spore solution to infect the trapped male flies.


Features

1. Specificity - Methyl Eugenol

Methyl Eugenol is the pheromone precursor of B. dorsalis. It only attracts male flies. The male flies will fly away once they have consumed enough Methyl Eugenol.

2. Target main Problem – Female B. dorsalis

female B. dorsalis poses more threat than males.

3. Regulation – IR Counter, Trap, Phone App

Automatically regulation of the spray frequency and door open duration depends on the data collected by the IR counter and phone APP.

4. Eco-Friendly – Recycle

Replacing the indissoluble plastic material (distributed by the government) with recyclable wood chips, which can reduce environmental pollution.

Mechanism


Functionality Display Video


IR Counter & APP


IR counter records and provides real-time information of the orchard : pest group size, meteorological data including temperature and humidity. With a smartphone app, the bait traps are able to inform the grower of these important information.

Design


Functional Test

To improve the accuracy of counter, we tried to find the proper tunnel diameter allows only one-by-one passage of B. dorsalis. We prepared different size of straws to connect two centrifuge tubes. One contained male B. dorsalis, and the other contained the bait, methyl eugenol.

Result

Results indicate that when the tunnel diameter is above 0.8 cm, the flies can pass through.

                                       
Diameter(cm)2.001.751.501.000.800.750.60
outcomeOOOOOHardlyX

Modeling Efficiency


A population size model of B. dorsalis was constructed to simulate a comparison of B. dorsalis population size between an IOS-protected orchard and a normal orchard. The results show a significant decrease of 35% in B. dorsalis population in an IOS-protected orchard compared to one without IOS.
Link to modeling

Population size without IOS



Population size with IOS



Expenditure Analysis


Based on our experimental deployment of counters and traps in an orchard one-hectare in size. The estimated total cost of IOS is listed below:

amount

cost (USD)

trap

4

60

counter

3

30

methyl eugenol

100 mL

50

total cost

90

It is estimated that orchard without any B. dorsalis treatment may incur lost up to 30% of fruit(1). A few calculation was done based on the data from government, and it shows that the saving of fruit value due to our IOS is much greater than the cost of IOS, suggesting our IOS is really worthwhile to be implemented.

(cost of IOS only in low ratio compared to the total profit)


(figure showing the great savings from IOS)


Reference:


1.http://www.mdais.gov.tw/files/web_articles_files/mdares/2469/631.pdf 2.http://ir.tari.gov.tw:8080/bitstream/345210000/281/1/57_1_5.pdf