Difference between revisions of "Team:Northwestern/06 27"

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     <h1>Monday, June 27<sup>th</sup></h3>
 
     <h1>Monday, June 27<sup>th</sup></h3>
 
     <h2>Agenda:</h2>
 
     <h2>Agenda:</h2>
−
 
+
<ul>
 +
      <li>Revise GenScript</li>
 +
      <li>Plate-read Interlab</li>
 +
      <li>Competent Cell efficiency test</li>
 +
      <li>Practice cloning</li>
 +
      <li>Take an inventory on the freezer</li>
 +
      <li>Make more LB </li>
 +
    </ul>
 +
<h2>Tasks:</h2>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Emailed Dr. Zhang asking about rapamycin-inducible split Cas9</li>
 +
          <li>Got a reply! They’ll send us a plasmid and the gene map</li>
 +
          <li>Helped with constructs</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Paul</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Competent Cell test kit transformation</li>
 +
          <li>Research background information (other delivery methods) for the GenScript grant</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>GenScript</li>
 +
          <li>Comp cell efficiency test with Paul</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Revised GenScript</li>
 +
          <li>Some CSS research</li>
 +
          <li>Talked with Dr. Mordacq and Jordan about Interlab plate reading</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Ordered BB Primers</li>
 +
          <li>Began redesigning Cas9 split optimized for <em>E. coli</em></li>
 +
          <li>Checked against other saCas9 sequences</li>
 +
          <li>High-level design of all constructs</li>
 +
          <li>Added sources to Gen Script Doc</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Shu</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Made an inventory list of stuff in the freezer</li>
 +
          <li>Did some research for Genscript and helped edit it</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Edited GenScript</li>
 +
          <li>Tested 37&#176;C LUDOX</li>
 +
          <li>Emailed Purdue</li>
 +
          <li>Checked freezer for primers</li>
 +
          <li>Emailed Mitch about primers</li>
 +
          <li>Wrote bio</li>
 +
          <li>Read some of the source papers for the review paper that sparked our whole project + added them to Mendeley</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
</article>
 
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Latest revision as of 15:24, 18 October 2016

Notebook

Monday, June 27th

Agenda:

  • Revise GenScript
  • Plate-read Interlab
  • Competent Cell efficiency test
  • Practice cloning
  • Take an inventory on the freezer
  • Make more LB

Tasks:

Sara

  • Emailed Dr. Zhang asking about rapamycin-inducible split Cas9
  • Got a reply! They’ll send us a plasmid and the gene map
  • Helped with constructs

Paul

  • Competent Cell test kit transformation
  • Research background information (other delivery methods) for the GenScript grant

Tasfia

  • GenScript
  • Comp cell efficiency test with Paul

Michelle

  • Revised GenScript
  • Some CSS research
  • Talked with Dr. Mordacq and Jordan about Interlab plate reading

Tyler

  • Ordered BB Primers
  • Began redesigning Cas9 split optimized for E. coli
  • Checked against other saCas9 sequences
  • High-level design of all constructs
  • Added sources to Gen Script Doc

Shu

  • Made an inventory list of stuff in the freezer
  • Did some research for Genscript and helped edit it

Sam

  • Edited GenScript
  • Tested 37°C LUDOX
  • Emailed Purdue
  • Checked freezer for primers
  • Emailed Mitch about primers
  • Wrote bio
  • Read some of the source papers for the review paper that sparked our whole project + added them to Mendeley