Difference between revisions of "Team:Northwestern/07 14"

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   <article>
 
   <article>
 
     <h1>Thursday, July 14<sup>th</sup></h3>
 
     <h1>Thursday, July 14<sup>th</sup></h3>
−
     <h2>Agenda:</h2>
+
     <h2>Tasks:</h2>
−
 
+
<div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked on CSS on menu</li>
 +
          <li>Studied javascript</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Paul</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Investigated DNA gels</li>
 +
      <ul><li>Made gels using our TAE &amp; our agarose, Kelly’s TAE &amp; agarose, our TAE &amp; Kelly’ agarose, Kelly’s TAE &amp; our agarose</li>
 +
      <li>Our agarose is the problem</li>
 +
      <li>3 batches of agarose made from the 3 bottles in the lab</li>
 +
      <li>We’ve been using Low Melting Point agarose by mistake</li></ul>
 +
      <li>Prepared miniprep culture </li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Sam</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Learned even more CSS</li>
 +
      <li>Started learning JavaScript </li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Sara </p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Miniprepped the pSB1C3-Tet plasmid DNA that Paul grew up the previous night</li>
 +
      <ul><li>Ran a miniprep for each tube of culture, one with water and one with elution buffer</li>
 +
      <li>89 ng/uL with water elution, 25 ng/uL with elution buffer elution</li></ul>
 +
      <li>Made 3 small test containers of 1% agarose</li>
 +
      <li>Made a Primers and Parts tab in the lab notebook </li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Shu</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Investigated gel</li>
 +
      <li>Edited the assembled parts</li>
 +
      <li>Learned how to make animation of proposed pathways </li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Tasfia</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Miniprepped the pSB1C3-Tet plasmid DNA that Paul grew up the previous night</li>
 +
      <ul>
 +
        <li>Ran a miniprep for each tube of culture, one with water and one with elution buffer</li>
 +
        <li>89 ng/uL with water elution, 25 ng/uL with elution buffer elution</li>
 +
      </ul>
 +
      <li>Tested different containers of agar for use in 1% agarose gels</li>
 +
      <li>Published a note on Experiment</li>
 +
      <li>Wrote more content drafts for website</li>
 +
      <li>Made a list of graphics we need</li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Tyler</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Finished mRFP and mRFP-gRNA constructs, sent them to grad students</li>
 +
      <li>Sponsor communication (Bio-rad and Qiagen)</li>
 +
      <li>Created meeting powerpoint for tomorrow</li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
  </article>
 
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/07_13"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
−
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calender </a></div>
+
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calendar </a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/07_15">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/07_15">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
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Latest revision as of 15:26, 18 October 2016

Notebook

Thursday, July 14th

Tasks:

Michelle

  • Worked on CSS on menu
  • Studied javascript

Paul

  • Investigated DNA gels
    • Made gels using our TAE & our agarose, Kelly’s TAE & agarose, our TAE & Kelly’ agarose, Kelly’s TAE & our agarose
    • Our agarose is the problem
    • 3 batches of agarose made from the 3 bottles in the lab
    • We’ve been using Low Melting Point agarose by mistake
  • Prepared miniprep culture

Sam

  • Learned even more CSS
  • Started learning JavaScript

Sara

  • Miniprepped the pSB1C3-Tet plasmid DNA that Paul grew up the previous night
    • Ran a miniprep for each tube of culture, one with water and one with elution buffer
    • 89 ng/uL with water elution, 25 ng/uL with elution buffer elution
  • Made 3 small test containers of 1% agarose
  • Made a Primers and Parts tab in the lab notebook

Shu

  • Investigated gel
  • Edited the assembled parts
  • Learned how to make animation of proposed pathways

Tasfia

  • Miniprepped the pSB1C3-Tet plasmid DNA that Paul grew up the previous night
    • Ran a miniprep for each tube of culture, one with water and one with elution buffer
    • 89 ng/uL with water elution, 25 ng/uL with elution buffer elution
  • Tested different containers of agar for use in 1% agarose gels
  • Published a note on Experiment
  • Wrote more content drafts for website
  • Made a list of graphics we need

Tyler

  • Finished mRFP and mRFP-gRNA constructs, sent them to grad students
  • Sponsor communication (Bio-rad and Qiagen)
  • Created meeting powerpoint for tomorrow