Difference between revisions of "Team:Northwestern/09 02"

 
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   <article>
 
   <article>
 
     <h1>Friday, September 2<sup>nd</sup></h3>
 
     <h1>Friday, September 2<sup>nd</sup></h3>
 
+
<h2>Transformation Results:</h2>
 
+
    <p>MIT plasmids transformed.</p>
 +
    <div class="row">
 +
      <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/6/62/T--Northwestern--09_02_1.jpg" width="460" height="464" alt=""/>
 +
        <p><strong>Figure 1:</strong> MIT pX851</p>
 +
      </div>
 +
      <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/1/15/T--Northwestern--09_02_2.jpg" width="478" height="469" alt=""/>
 +
        <p><strong>Figure 2:</strong> MIT pX852</p>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/7/75/T--Northwestern--09_02_3.jpg" width="1601" height="1615" alt=""/>
 +
        <p><strong>Figure 3:</strong> Positive transformation control</p>
 +
      </div>
 +
</div>
 +
<h2>Tasks:</h2>
 +
<div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Miniprepped Cas9 and gRNA template plasmid</li>
 +
          <ul><li>Cas9 tube 1: 231.7 ng/uL, 260/280: 1.89, 260/230: 2.16</li>
 +
          <li>Cas9 tube 2: 358.9 ng/uL, 260/280: 1.88, 260/230: 2.30</li>
 +
          <li>gRNA tube 1: 174.7 ng/uL, 260/280: 1.91, 260/230: 2.26</li>
 +
          <li>gRNA tube 2: 235.4 ng/uL, 260/280: 1.90, 260/230: 2.25</li>
 +
          <li>gRNA tube 3: 223.8 ng/uL, 260/280: 1.90, 260/230: 2.23</li></ul>
 +
          <li>Made Tet+Cam plates:</li>
 +
          <ul><li>230 mL LB + 3.4510 g Bacto Agar </li></ul>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked on Auburn Gresham plan</li>
 +
          <li>Added some website content</li>
 +
          <li>Edited adult pamphlet </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked on lab notebook</li>
 +
          <li>Ran a gel on the Cas9 insert with the added restriction sites and on the Cas9-linearized for SS with the extra homology</li>
 +
          <ul><li>3 uL SybrSafe</li>
 +
          <li>Ladder, Cas9-1, Cas9-1, Cas9-2, Cas9-2, space, Cas9SS-1, Cas9SS-1, Cas9SS-2, Cas9SS-2</li>
 +
          <li>Used 5 uL 1 kb ladder and 4 uL dye </li></ul>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
</article>
 
<footer id="nav">
 
<footer id="nav">
 
       <div class="row">
 
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_01"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_01"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calender </a></div>
+
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calendar </a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_03">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_03">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
       </div>
 
       </div>

Latest revision as of 15:34, 18 October 2016

Notebook

Friday, September 2nd

Transformation Results:

MIT plasmids transformed.

Figure 1: MIT pX851

Figure 2: MIT pX852

Figure 3: Positive transformation control

Tasks:

Michelle

  • Miniprepped Cas9 and gRNA template plasmid
    • Cas9 tube 1: 231.7 ng/uL, 260/280: 1.89, 260/230: 2.16
    • Cas9 tube 2: 358.9 ng/uL, 260/280: 1.88, 260/230: 2.30
    • gRNA tube 1: 174.7 ng/uL, 260/280: 1.91, 260/230: 2.26
    • gRNA tube 2: 235.4 ng/uL, 260/280: 1.90, 260/230: 2.25
    • gRNA tube 3: 223.8 ng/uL, 260/280: 1.90, 260/230: 2.23
  • Made Tet+Cam plates:
    • 230 mL LB + 3.4510 g Bacto Agar

Sam

  • Worked on Auburn Gresham plan
  • Added some website content
  • Edited adult pamphlet

Sara

  • Worked on lab notebook
  • Ran a gel on the Cas9 insert with the added restriction sites and on the Cas9-linearized for SS with the extra homology
    • 3 uL SybrSafe
    • Ladder, Cas9-1, Cas9-1, Cas9-2, Cas9-2, space, Cas9SS-1, Cas9SS-1, Cas9SS-2, Cas9SS-2
    • Used 5 uL 1 kb ladder and 4 uL dye