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| <article> | | <article> |
| <h1>Friday, September 2<sup>nd</sup></h3> | | <h1>Friday, September 2<sup>nd</sup></h3> |
− | | + | <h2>Transformation Results:</h2> |
− | | + | <p>MIT plasmids transformed.</p> |
| + | <div class="row"> |
| + | <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/6/62/T--Northwestern--09_02_1.jpg" width="460" height="464" alt=""/> |
| + | <p><strong>Figure 1:</strong> MIT pX851</p> |
| + | </div> |
| + | <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/1/15/T--Northwestern--09_02_2.jpg" width="478" height="469" alt=""/> |
| + | <p><strong>Figure 2:</strong> MIT pX852</p> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/7/75/T--Northwestern--09_02_3.jpg" width="1601" height="1615" alt=""/> |
| + | <p><strong>Figure 3:</strong> Positive transformation control</p> |
| + | </div> |
| + | </div> |
| + | <h2>Tasks:</h2> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Michelle</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Miniprepped Cas9 and gRNA template plasmid</li> |
| + | <ul><li>Cas9 tube 1: 231.7 ng/uL, 260/280: 1.89, 260/230: 2.16</li> |
| + | <li>Cas9 tube 2: 358.9 ng/uL, 260/280: 1.88, 260/230: 2.30</li> |
| + | <li>gRNA tube 1: 174.7 ng/uL, 260/280: 1.91, 260/230: 2.26</li> |
| + | <li>gRNA tube 2: 235.4 ng/uL, 260/280: 1.90, 260/230: 2.25</li> |
| + | <li>gRNA tube 3: 223.8 ng/uL, 260/280: 1.90, 260/230: 2.23</li></ul> |
| + | <li>Made Tet+Cam plates:</li> |
| + | <ul><li>230 mL LB + 3.4510 g Bacto Agar </li></ul> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Sam</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Worked on Auburn Gresham plan</li> |
| + | <li>Added some website content</li> |
| + | <li>Edited adult pamphlet </li> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | <div class="row"> |
| + | <div class="col-sm-2"> |
| + | <p>Sara</p> |
| + | </div> |
| + | <div class="col-sm-10"> |
| + | <ul> |
| + | <li>Worked on lab notebook</li> |
| + | <li>Ran a gel on the Cas9 insert with the added restriction sites and on the Cas9-linearized for SS with the extra homology</li> |
| + | <ul><li>3 uL SybrSafe</li> |
| + | <li>Ladder, Cas9-1, Cas9-1, Cas9-2, Cas9-2, space, Cas9SS-1, Cas9SS-1, Cas9SS-2, Cas9SS-2</li> |
| + | <li>Used 5 uL 1 kb ladder and 4 uL dye </li></ul> |
| + | </ul> |
| + | </div> |
| + | </div> |
| + | </article> |
| <footer id="nav"> | | <footer id="nav"> |
| <div class="row"> | | <div class="row"> |
| <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_01"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div> | | <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_01"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div> |
− | <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calender </a></div> | + | <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calendar </a></div> |
| <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_03">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div> | | <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_03">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div> |
| </div> | | </div> |