Difference between revisions of "Team:Northwestern/09 17"

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   <article>
 
   <article>
 
     <h1>Saturday, September 17<sup>th</sup></h3>
 
     <h1>Saturday, September 17<sup>th</sup></h3>
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    <h2>Agenda:</h2>
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<h2>Tasks:</h2>
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<div class="row">
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      <div class="col-sm-2">
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        <p>Sam</p>
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      </div>
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      <div class="col-sm-10">
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        <ul>
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          <li>Gel screen of pET28a</li>
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          <ul><li>5 μL SybrSafe (Mistake. Should have added 3 μL.)</li>
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          <li>6 μL 1kb ladder + 3 μL 6X purple loading dye</li>
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          <li>In each sample, 1 μL 6X purple loading dye + 5 μL DNA </li>
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          <div class="row">
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              <div class="col-xs-6 col-sm-8"><img src="https://static.igem.org/mediawiki/2016/0/00/T--Northwestern--09_17_1.png" width="1006" height="260" alt=""/></div>
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              <p>Size of pet28a: 5369 bases</p>
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            </div>
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            <li>PCR purification of pET28a: Conc: 120.3 ng/μL, 260/280: 1.87, 260/230: 1.83</li>
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          </ul>
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        </ul>
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      </div>
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    </div>
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</article>
 
<footer id="nav">
 
<footer id="nav">
 
       <div class="row">
 
       <div class="row">
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_16"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_16"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calender </a></div>
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calendar </a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_18">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_18">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
       </div>
 
       </div>
 
     </footer>
 
     </footer>
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  </article>
 
 
<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>
 
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Latest revision as of 15:36, 18 October 2016

Notebook

Saturday, September 17th

Tasks:

Sam

  • Gel screen of pET28a
    • 5 μL SybrSafe (Mistake. Should have added 3 μL.)
    • 6 μL 1kb ladder + 3 μL 6X purple loading dye
    • In each sample, 1 μL 6X purple loading dye + 5 μL DNA

      • Size of pet28a: 5369 bases

    • PCR purification of pET28a: Conc: 120.3 ng/μL, 260/280: 1.87, 260/230: 1.83