Difference between revisions of "Team:Northwestern/09 27"

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   <article>
 
   <article>
 
     <h1>Tuesday, September 27<sup>th</sup></h3>
 
     <h1>Tuesday, September 27<sup>th</sup></h3>
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    <h2>Agenda:</h2>
+
<h2>Tasks:</h2>
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+
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Jordan</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>DpnI digest of SS-Cas9 PCR</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Paul</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>PCR of all SS-Cas9 with new primers</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul><li>Ran a gel screen on Cas9 and SS with restriction digest ends for assembly into pSB1C3 plasmid</li>
 +
        <li>It did not work</li></ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Retransform all pET28a Parts (edit: just use colonies from last time, using a combination of colony pcr and restriction digest to figure out which colonies match up with which sequencing prep)</li>
 +
          <li>Grow up cultures of pET28a parts</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Pick Up Primers</li>
 +
          <li>Take cas9+gRNA cultures out of shaking incubator </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
  </article>
 
<footer id="nav">
 
<footer id="nav">
 
       <div class="row">
 
       <div class="row">
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_26"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_26"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
−
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calender </a></div>
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calendar </a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_28">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/09_28">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
       </div>
 
       </div>
 
     </footer>
 
     </footer>
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  </article>
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<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>
 
         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>

Latest revision as of 15:37, 18 October 2016

Notebook

Tuesday, September 27th

Tasks:

Jordan

  • DpnI digest of SS-Cas9 PCR

Paul

  • PCR of all SS-Cas9 with new primers

Sara

  • Ran a gel screen on Cas9 and SS with restriction digest ends for assembly into pSB1C3 plasmid
  • It did not work

Tasfia

  • Retransform all pET28a Parts (edit: just use colonies from last time, using a combination of colony pcr and restriction digest to figure out which colonies match up with which sequencing prep)
  • Grow up cultures of pET28a parts

Tyler

  • Pick Up Primers
  • Take cas9+gRNA cultures out of shaking incubator