Difference between revisions of "Team:Northwestern/10 06"

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   <article>
 
     <h1>Thursday, October 6<sup>th</sup></h3>
 
     <h1>Thursday, October 6<sup>th</sup></h3>
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    <h2>Agenda:</h2>
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<h2>Tasks:</h2>
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<div class="row">
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      <div class="col-sm-2">
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        <p>Jordan</p>
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      </div>
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      <div class="col-sm-10">
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        <ul>
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          <li>Grow up cultures Western blot cultures to OD=0.6 (15-25 mL)</li>
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          <li>JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP</li>
 +
          <li>BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9 (15 mL)</li>
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          <li>Periplasm procedure on all Western blot cultures</li>
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          <li>Clean out fridge </li>
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        </ul>
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      </div>
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    </div>
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<div class="row">
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  <div class="col-sm-2">
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    <p>Sam &amp; Tasfia</p>
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  </div>
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  <div class="col-sm-10">
 +
    <ul>
 +
      <li>OMV purification of JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP </li>
 +
    </ul>
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  </div>
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</div>
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<div class="row">
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  <div class="col-sm-2">
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    <p>Tasfia, Shu, &amp; Sara</p>
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  </div>
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  <div class="col-sm-10">
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    <ul>
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      <li>Start Western blot procedure (run protein gel, load nitrocellulose membrane, start blocking overnight) </li>
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    </ul>
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  </div>
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</div>
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  </article>
  
 
<footer id="nav">
 
<footer id="nav">
 
       <div class="row">
 
       <div class="row">
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/10_05"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/10_05"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Northwestern--backarrow.png" height="15" width="15"/> yesterday</a></div>
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calender </a></div>
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         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/Notebook">back to calendar </a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/10_07">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
         <div class="col-sm-4"><a href="https://2016.igem.org/Team:Northwestern/10_07">tomorrow <img src="https://static.igem.org/mediawiki/2016/6/65/T--Northwestern--forwardarrow.png" height="15" width="15"/></a></div>
 
       </div>
 
       </div>

Latest revision as of 15:38, 18 October 2016

Notebook

Thursday, October 6th

Tasks:

Jordan

  • Grow up cultures Western blot cultures to OD=0.6 (15-25 mL)
  • JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP
  • BL21 (DE3) pET28a-SS-Cas9 (TorA, DsbA, YcdO), Cas9 (15 mL)
  • Periplasm procedure on all Western blot cultures
  • Clean out fridge

Sam & Tasfia

  • OMV purification of JC8031 w/ pSB1C3-SS-Cas9 (TorA, DsbA, YcdO), Cas9, ClyA-GFP

Tasfia, Shu, & Sara

  • Start Western blot procedure (run protein gel, load nitrocellulose membrane, start blocking overnight)