Difference between revisions of "Team:NRP-UEA-Norwich/Parts"
| Line 22: | Line 22: | ||
<td>Hyd C (Fdh)</td> | <td>Hyd C (Fdh)</td> | ||
<td>FeFe hydrogenase outer periplasmic subunit</td> | <td>FeFe hydrogenase outer periplasmic subunit</td> | ||
| − | <td> | + | <td>705</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 29: | Line 29: | ||
<td>Hyd B (SII C-terminus)</td> | <td>Hyd B (SII C-terminus)</td> | ||
<td>FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus</td> | <td>FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus</td> | ||
| − | <td> | + | <td>374</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 36: | Line 36: | ||
<td>Hyd B (SII N-terminus)</td> | <td>Hyd B (SII N-terminus)</td> | ||
<td>FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus</td> | <td>FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus</td> | ||
| − | <td> | + | <td>374</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 42: | Line 42: | ||
<td>pSB1C3</td> | <td>pSB1C3</td> | ||
<td>Hyd A</td> | <td>Hyd A</td> | ||
| − | <td>FeFe hydrogenase membrane bound | + | <td>FeFe hydrogenase membrane bound catalytic subunit</td> |
| − | <td> | + | <td>1266</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 50: | Line 50: | ||
<td>Hya A</td> | <td>Hya A</td> | ||
<td>NiFe hydrogenase central subunit</td> | <td>NiFe hydrogenase central subunit</td> | ||
| − | <td> | + | <td>1156</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 57: | Line 57: | ||
<td>Hya C</td> | <td>Hya C</td> | ||
<td>NiFe hydrogenase outer periplasmic subunit</td> | <td>NiFe hydrogenase outer periplasmic subunit</td> | ||
| − | <td> | + | <td>695</td> |
<tr> | <tr> | ||
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957007 BBa_K1957007] </span></td> | <td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957007 BBa_K1957007] </span></td> | ||
| Line 63: | Line 63: | ||
<td>Hya B (SII C-terminus)</td> | <td>Hya B (SII C-terminus)</td> | ||
<td>NiFe hydrogenase membrane bound catalytic subunit</td> | <td>NiFe hydrogenase membrane bound catalytic subunit</td> | ||
| − | <td> | + | <td>1771</td> |
<tr> | <tr> | ||
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957008 BBa_K1957008] </span></td> | <td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957008 BBa_K1957008] </span></td> | ||
| Line 69: | Line 69: | ||
<td>Hya B (SII N-terminus)</td> | <td>Hya B (SII N-terminus)</td> | ||
<td>NiFe hydrogenase membrane bound catalytic subunit</td> | <td>NiFe hydrogenase membrane bound catalytic subunit</td> | ||
| − | <td> | + | <td>1771</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 15:53, 18 October 2016
Parts
All BioBricks consist of basic Ribosomal Binding Sites (RBS) parts ligated with the pSB1C3 iGEM vector. All BioBricks samples have been tested for accuracy by DNA sequencing. All gene parts in these BioBricks originated from the organism Shewanella oneidensis and were submitted to the iGEM registry on the 19th of October.
| Name in Registry | Backbone | Gene expressed | Function | Length (bp) |
|---|---|---|---|---|
| [http://parts.igem.org/Part:BBa_K1957001 BBa_K1957001] | pSB1C3 | Hyd C (Fdh) | FeFe hydrogenase outer periplasmic subunit | 705 |
| [http://parts.igem.org/Part:BBa_K1957002 BBa_K1957002] | pSB1C3 | Hyd B (SII C-terminus) | FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus | 374 |
| [http://parts.igem.org/Part:BBa_K1957003 BBa_K1957003] | pSB1C3 | Hyd B (SII N-terminus) | FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus | 374 |
| [http://parts.igem.org/Part:BBa_K1957004 BBa_K1957004] | pSB1C3 | Hyd A | FeFe hydrogenase membrane bound catalytic subunit | 1266 |
| [http://parts.igem.org/Part:BBa_K1957005 BBa_K1957005] | pSB1C3 | Hya A | NiFe hydrogenase central subunit | 1156 |
| [http://parts.igem.org/Part:BBa_K1957006 BBa_K1957006] | pSB1C3 | Hya C | NiFe hydrogenase outer periplasmic subunit | 695 |
| [http://parts.igem.org/Part:BBa_K1957007 BBa_K1957007] | pSB1C3 | Hya B (SII C-terminus) | NiFe hydrogenase membrane bound catalytic subunit | 1771 |
| [http://parts.igem.org/Part:BBa_K1957008 BBa_K1957008] | pSB1C3 | Hya B (SII N-terminus) | NiFe hydrogenase membrane bound catalytic subunit | 1771 |
| [http://parts.igem.org/Part:BBa_K1957009 BBa_K1957009] | pSB1C3 | HydABC, FeFe Hydrogenase gene cluster (SII N-terminus) | FeFe hydrogenase gene cluster. Strep II tag incorporated onto the N-terminus | 2291 |
