Difference between revisions of "Team:BGIC China/Collaborations"
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{{BGIC_China}} | {{BGIC_China}} | ||
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Here is a link to their collaboration page: \u003ca target=\"_blank\" href=\"https://2016.igem.org/Team:Shenzhen_SFLS/Collaborations\"\u003ehttps://2016.igem.org/Team:Shenzhen_SFLS/Collaborations\u003c/a\u003e\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Separator","id":"f_fd2ef77d-575c-47c7-b1e5-81cd7b041abc","defaultValue":null,"value":null},{"type":"RichText","id":"f_703c93a4-9aa5-4733-8f12-d0f2ac4920e9","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\"\u003e\u003cstrong\u003eHere is an abstract from SFLS team:\u003c/strong\u003e\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"RichText","id":"f_50698aaf-ec8c-4aee-abe9-c02f6350f723","defaultValue":false,"value":"\u003cp class=\"s-text-color-blue\" style=\"font-size: 130%;\"\u003e\u003cu\u003eTeam BGIC China\u0026gt;\u0026gt;Us\u003c/u\u003e\u003c/p\u003e\u003cp class=\"s-text-color-blue\" style=\"font-size: 130%;\"\u003e \u003c/p\u003e\u003cp class=\"s-text-color-blue\" style=\"font-size: 130%;\"\u003eTeam BGIC China were working on a programmable and open-source Next Generation Bio-Chip (NGBC) which allows user with knowledge in synthetic biology to easily re-program and re-construct it in order to make new bio-chips with different functions. 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Here is their result.\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"BlockComponentItem","id":"f_5e587694-6344-4189-8001-9f7d1a44b325","defaultValue":false,"name":"smallFeatureLeft","components":{"media1":{"type":"Media","id":"f_915da018-1925-4f37-8a0c-6da69c40ed85","defaultValue":true,"video":{"type":"Video","id":"f_d273adcc-407e-4fb0-a31a-abcb20b6322c","defaultValue":null,"html":"","url":"","thumbnail_url":null,"maxwidth":700,"description":null},"image":{"type":"Image","id":"f_ab0d20f2-2025-43df-b608-8c482f7a6729","defaultValue":false,"link_url":"","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/1_zuatsg","storage":"c","format":"jpg","h":2714,"w":2048,"s":611126,"new_target":true},"current":"image"},"text1":{"type":"RichText","id":"f_1e782f9f-6548-4004-ad0f-5d230471613c","defaultValue":false,"value":"","backupValue":"标题文本","version":1},"text2":{"type":"RichText","id":"f_1def6189-91cb-446e-bc13-8f6603228b4a","defaultValue":false,"value":"","backupValue":"小标语","version":1},"text3":{"type":"RichText","id":"f_9c0d3ae9-dcd5-4fc7-a134-849c6daa3681","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\" class=\"s-text-color-blue\"\u003eThe blue liquid in the small eppendorf is the S30 system with gene circuit coding enzyme b-galactosidase (LacZ) and its substrates, X-gal, after hours of reaction.\u003c/p\u003e\u003cp style=\"font-size: 130%;\" class=\"s-text-color-blue\"\u003eIt qualitatively proved that our S30 cell-free system works well.\u003c/p\u003e","backupValue":"简介你的项目或成员。夏天的飞鸟，飞到我的窗前唱歌，又飞去了。秋天的黄叶，它们没有什么可唱，只叹息一声，飞落在那里。","version":1}}},{"type":"RichText","id":"f_17123c21-0016-401e-be3e-f0a69a201d26","defaultValue":false,"value":"\u003cp class=\"s-text-color-blue\"\u003e\u003cem\u003e\u003cstrong\u003eFig.1 Test result in S30 cell-free system\u003c/strong\u003e\u003c/em\u003e\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"RichText","id":"f_7aa78750-1699-496a-b808-255c73e858ee","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\" class=\"s-text-color-blue\"\u003eWe also tested this with a different reporter gene, luciferase. Check our results in the “Proof of Concept” section of our wiki.\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"RichText","id":"f_d6f927d6-c659-4621-b276-afabf83d8171","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\" class=\"s-text-color-blue\"\u003e\u003cu\u003eSFLS\u0026gt;\u0026gt;Team BGIC China\u003c/u\u003e\u003c/p\u003e\u003cp style=\"font-size: 130%;\" class=\"s-text-color-blue\"\u003e \u003c/p\u003e\u003cp style=\"font-size: 130%;\" class=\"s-text-color-blue\"\u003eWe helped Team BGIC China by constructing plasmid pfadr for them. Plasmid pfadr is already in the parts collection and is modified to be responsive to fatty acid. Here are some of the results we got in the process.\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_9d72aa43-f637-4bc4-b684-ac57306fdd3f","defaultValue":false,"link_url":"","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/2_avgq0n","storage":"c","format":"jpg","h":2714,"w":2048,"s":708047,"new_target":true},{"type":"RichText","id":"f_c9136345-3341-4d4e-b781-dfbd941acfe2","defaultValue":false,"value":"\u003cp class=\"s-text-color-blue\"\u003e\u003cstrong\u003e\u003cem\u003eFig.2 The Nanodrop results of plasmid extraction\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_c12a3d63-baa3-4f5d-869b-1ba23303ba0e","defaultValue":false,"link_url":"","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/3_fdonlt","storage":"c","format":"jpg","h":2714,"w":2048,"s":696807,"new_target":true},{"type":"RichText","id":"f_0e275c2a-ff54-477e-8069-6112d7ae06f8","defaultValue":false,"value":"\u003cp class=\"s-text-color-blue\"\u003e\u003cem\u003e\u003cstrong\u003eFig.3 The PCR result\u003c/strong\u003e\u003c/em\u003e\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"RichText","id":"f_1df41263-71b5-4ee5-a303-24b4ac28d7b1","defaultValue":false,"value":"\u003cp class=\"s-text-color-blue\" style=\"font-size: 130%;\"\u003eWe have really close relationship with Team BGIC China, because we share the same laboratory with them and received training on the cloning cycled together. We also shared a great part of cloning materials in the beginning of our experiment because our materials haven’t arrived yet. We always had great communication about our projects and on our testing experiments, since our projects are based on the same technology platform-cell-free system.\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1}]}}}],"title":null,"uid":null,"path":null,"autoPath":null}],"menu":{"type":"Menu","id":"f_54310d53-5ba2-4793-bd59-b00da4081629","defaultValue":null,"template_name":"navbar","logo":null,"components":{"background1":{"type":"Background","id":"f_76c941dc-c059-4061-ba2c-b0642a1f396b","defaultValue":true,"url":"/assets/themes/profile/bg.jpg","textColor":"light","backgroundVariation":"","sizing":"cover","videoUrl":"","videoHtml":"","storageKey":null,"storage":null,"format":null,"h":null,"w":null,"s":null},"image1":{"type":"Image","id":"f_a8010fa7-c561-4eea-8293-793825093faf","defaultValue":false,"link_url":"","thumb_url":"/assets/icons/transparent.png","url":"/assets/icons/transparent.png","caption":"","description":"","storageKey":null,"storage":null,"format":null,"h":null,"w":null,"s":null,"new_target":true},"image2":{"type":"Image","id":"f_8e37c922-ca0c-408d-9623-00fd9782d212","defaultValue":true,"link_url":null,"thumb_url":null,"url":"http://assets.strikingly.com/assets/themes/fresh/power.png","caption":"","description":"","storageKey":null,"storage":null,"format":null,"h":null,"w":null,"s":null,"new_target":true},"text1":{"type":"RichText","id":"f_b91c547a-71fd-412c-bbe0-ae1233468cf3","defaultValue":false,"value":"","backupValue":"Title Text","version":1},"text2":{"type":"RichText","id":"f_ddec1bbb-3d45-468d-9a29-ec30cb3ae201","defaultValue":false,"value":"","backupValue":"Subtitle Text","version":1},"button1":{"type":"Button","id":"f_81f80351-4ea1-4e4e-b0e9-c09e7342fd3d","defaultValue":false,"text":"","url":"","new_target":null}}},"footer":{"type":"Footer","id":"f_2d75fc34-40d5-416b-bb48-e437be8b670c","defaultValue":null,"socialMedia":null,"copyright":null,"components":{"socialMedia":{"type":"SocialMediaList","id":"f_52dc7699-9380-4e8d-9500-e51618c95e8f","defaultValue":false,"link_list":[{"type":"Facebook","id":"f_90b4be82-1a25-42d9-8160-4aa2fa7c48a9","defaultValue":null,"url":"","link_url":null,"share_text":null,"app_id":null,"show_button":true},{"type":"Twitter","id":"f_85287602-d59a-43fd-8845-fac8161206bc","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":true},{"type":"GPlus","id":"f_d4e77048-3568-4f35-b0cd-cbe57eeee145","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false},{"type":"LinkedIn","id":"f_b01e265e-b136-4725-9cde-97ef60d0261e","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false},{"type":"Instagram","id":"f_118b9462-0d12-4ec5-9d5a-95f2af2f53fa","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false}],"button_list":[{"type":"Facebook","id":"f_55cb59a1-a151-4ade-bd94-cd8022bf954b","defaultValue":null,"url":"","link_url":"","share_text":"","app_id":543870062356274,"show_button":true},{"type":"Twitter","id":"f_48056a2f-5715-4d2a-8523-3a6ad0f2cbd0","defaultValue":null,"url":"","link_url":"","share_text":"","show_button":true},{"type":"GPlus","id":"f_31a4f1f7-2a6d-4159-90c7-121a859ce637","defaultValue":null,"url":"","link_url":"","share_text":"","show_button":true},{"type":"LinkedIn","id":"f_37c15ea1-fafe-4ce5-b15d-01a359744f1c","defaultValue":null,"url":"","link_url":"","share_text":"","show_button":false},{"type":"Pinterest","id":"f_0e26ea8c-251c-414f-bf45-f3dd324cba3e","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false}],"list_type":null},"copyright":{"type":"RichText","id":"f_287cb485-8a5e-43d3-9cfb-25574bc440fe","defaultValue":false,"value":"\u003cp\u003eCopyright © 2002 - 2016 Eckhart Consulting. 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| − | + | </script> <!--[if lte IE 9]><script src="//assets.strikingly.com/assets/detectIE-b0fc7986f8eb7d40a31e9582bdeb7b88.js"></script><![endif]--> </head> <body class='show locale-zh-CN' id='user_sites'> <div id="s-page-container"><div data-reactroot="" data-reactid="1" data-react-checksum="698187675"><!-- react-empty: 2 --><div id="s-content" class="s-variation-default s-font-heading-default s-font-title-default s-font-body-default" data-reactid="3"><div class="social-thumbnail" data-reactid="4"><div style="display:none;" data-reactid="5"><img src="//assets.strikingly.com/assets/fb_images/default-715654470f8f9a657474bf68cefc382f.png" alt="page thumbnail" width="300" height="300" data-reactid="6"/></div><div id="lw-share-data" style="display:none;" data-reactid="7"><div class="lws-title" data-reactid="8">Ellz's Site Copy</div><div class="lws-description" data-reactid="9">Website</div><div class="lws-image" data-reactid="10">http://assets.strikingly.com/assets/fb_images/default-715654470f8f9a657474bf68cefc382f.png</div></div></div><!-- react-empty: 11 --><!-- react-empty: 12 --><ul class="slides s-page-1" data-reactid="13"><li class="slide s-section-1" id="section-f_a59164e3-0093-46ee-aedf-cb5fba5e8347" data-reactid="14"><div class="waypoint" data-reactid="15"></div><a class="section-anchor" data-reactid="16"></a><div class="lazyload s-block-section s-section" data-reactid="17"><div class="container" data-reactid="18"><div class="columns sixteen" data-reactid="19"><!-- react-empty: 20 --></div><div class="alignment-container " data-reactid="21"><div class="s-repeatable s-block s-component s-mh " data-reactid="22"><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-title " data-reactid="23"><span data-reactid="24"><div data-reactid="25"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="26"><div class=" columns sixteen" data-reactid="27"><div class="s-item-title" data-reactid="28"><div class="s-component s-text" data-reactid="29"><h3 class="s-component-content s-font-heading" data-reactid="30"><p style="font-size: 160%;">Collaboration</p></h3></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="31"><span data-reactid="32"><div data-reactid="33"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="34"><div class=" columns sixteen" data-reactid="35"><div class="s-component s-text" data-reactid="36"><div class="s-component-content s-font-body" data-reactid="37"><p style="font-size:130%">Since SFLS team and us both used the S30 cell-free system in our projects, so we assisted each other on different aspects. Our team helped SFLS team to validate the viability of gene expression in S30 cell-free system. Here is a link to their collaboration page: <a target="_blank" href="https://2016.igem.org/Team:Shenzhen_SFLS/Collaborations">https://2016.igem.org/Team:Shenzhen_SFLS/Collaborations</a></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="38"><span data-reactid="39"><div data-reactid="40"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="41"><div class=" columns sixteen" data-reactid="42"><div class="s-separator" data-reactid="43"><div class="s-separator-content" data-reactid="44"><div class="separator" data-reactid="45"></div></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="46"><span data-reactid="47"><div data-reactid="48"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="49"><div class=" columns sixteen" data-reactid="50"><div class="s-component s-text" data-reactid="51"><div class="s-component-content s-font-body" data-reactid="52"><p style="font-size: 130%;"><strong>Here is an abstract from SFLS team:</strong></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="53"><span data-reactid="54"><div data-reactid="55"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="56"><div class=" columns sixteen" data-reactid="57"><div class="s-component s-text" data-reactid="58"><div class="s-component-content s-font-body" data-reactid="59"><p class="s-text-color-blue" style="font-size: 130%;"><u>Team BGIC China>>Us</u></p><p class="s-text-color-blue" style="font-size: 130%;"> </p><p class="s-text-color-blue" style="font-size: 130%;">Team BGIC China were working on a programmable and open-source Next Generation Bio-Chip (NGBC) which allows user with knowledge in synthetic biology to easily re-program and re-construct it in order to make new bio-chips with different functions. They based their NGBC on <em>E. coli </em>S30 Extract System for Circular DNA(Promega), the same with ours. They also utilized enzyme b-galactosidase (LacZ) as one of their reporter gene. So they helped us to test whether S30 system was qualified as a platform for translation of enzyme b-galactosidase (LacZ). Here is their result.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-smallFeatureLeft " data-reactid="60"><span data-reactid="61"><div data-reactid="62"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="63"><div class="" data-reactid="64"><div class="s-block-feature " data-reactid="65"><div class="six columns" data-reactid="66"><div class="s-item-media-group" data-reactid="67"><div class="s-component s-media " data-reactid="68"><span data-reactid="69"><div class="s-component-content" data-reactid="70"><div data-reactid="71"><!-- react-text: 72 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:2048px;max-height:2714px;" data-reactid="73"><div class="s-ratio-fill" style="padding-bottom:132.52%;" data-reactid="74"></div><div data-reactid="75"><img alt="" title="" data-description="" width="2048" height="2714" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/1_zuatsg.jpg" data-sizes="auto" data-reactid="76"/><noscript data-reactid="77"><img alt="" title="" data-description="" width="2048" height="2714" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/1_zuatsg.jpg" class="" data-reactid="78"/></noscript></div></div><!-- react-text: 79 --> <!-- /react-text --></div></div></span></div></div></div><div class="ten columns" data-reactid="80"><div class="s-item-text-group half-offset-left" data-reactid="81"><div class="s-item-title" data-reactid="82"><div class="s-component s-text" data-reactid="83"></div></div><div class="s-item-subtitle" data-reactid="84"><div class="s-component s-text" data-reactid="85"></div></div><div class="s-item-text" data-reactid="86"><div class="s-component s-text" data-reactid="87"><div class="s-component-content s-font-body" data-reactid="88"><p style="font-size: 130%;" class="s-text-color-blue">The blue liquid in the small eppendorf is the S30 system with gene circuit coding enzyme b-galactosidase (LacZ) and its substrates, X-gal, after hours of reaction.</p><p style="font-size: 130%;" class="s-text-color-blue">It qualitatively proved that our S30 cell-free system works well.</p></div></div></div></div></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="89"><span data-reactid="90"><div data-reactid="91"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="92"><div class=" columns sixteen" data-reactid="93"><div class="s-component s-text" data-reactid="94"><div class="s-component-content s-font-body" data-reactid="95"><p class="s-text-color-blue"><em><strong>Fig.1 Test result in S30 cell-free system</strong></em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="96"><span data-reactid="97"><div data-reactid="98"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="99"><div class=" columns sixteen" data-reactid="100"><div class="s-component s-text" data-reactid="101"><div class="s-component-content s-font-body" data-reactid="102"><p style="font-size: 130%;" class="s-text-color-blue">We also tested this with a different reporter gene, luciferase. Check our results in the “Proof of Concept” section of our wiki.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="103"><span data-reactid="104"><div data-reactid="105"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="106"><div class=" columns sixteen" data-reactid="107"><div class="s-component s-text" data-reactid="108"><div class="s-component-content s-font-body" data-reactid="109"><p style="font-size: 130%;" class="s-text-color-blue"><u>SFLS>>Team BGIC China</u></p><p style="font-size: 130%;" class="s-text-color-blue"> </p><p style="font-size: 130%;" class="s-text-color-blue">We helped Team BGIC China by constructing plasmid pfadr for them. Plasmid pfadr is already in the parts collection and is modified to be responsive to fatty acid. Here are some of the results we got in the process.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="110"><span data-reactid="111"><div data-reactid="112"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="113"><div class=" columns sixteen" data-reactid="114"><div class="s-component s-image" data-reactid="115"><span data-reactid="116"><div class="s-component-content" data-reactid="117"><div data-reactid="118"><!-- react-text: 119 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:2048px;max-height:2714px;" data-reactid="120"><div class="s-ratio-fill" style="padding-bottom:132.52%;" data-reactid="121"></div><div data-reactid="122"><img alt="" title="" data-description="" width="2048" height="2714" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/2_avgq0n.jpg" data-sizes="auto" data-reactid="123"/><noscript data-reactid="124"><img alt="" title="" data-description="" width="2048" height="2714" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/2_avgq0n.jpg" class="" data-reactid="125"/></noscript></div></div><!-- react-text: 126 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="127"><span data-reactid="128"><div data-reactid="129"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="130"><div class=" columns sixteen" data-reactid="131"><div class="s-component s-text" data-reactid="132"><div class="s-component-content s-font-body" data-reactid="133"><p class="s-text-color-blue"><strong><em>Fig.2 The Nanodrop results of plasmid extraction</em></strong></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="134"><span data-reactid="135"><div data-reactid="136"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="137"><div class=" columns sixteen" data-reactid="138"><div class="s-component s-image" data-reactid="139"><span data-reactid="140"><div class="s-component-content" data-reactid="141"><div data-reactid="142"><!-- react-text: 143 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:2048px;max-height:2714px;" data-reactid="144"><div class="s-ratio-fill" style="padding-bottom:132.52%;" data-reactid="145"></div><div data-reactid="146"><img alt="" title="" data-description="" width="2048" height="2714" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/3_fdonlt.jpg" data-sizes="auto" data-reactid="147"/><noscript data-reactid="148"><img alt="" title="" data-description="" width="2048" height="2714" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/3_fdonlt.jpg" class="" data-reactid="149"/></noscript></div></div><!-- react-text: 150 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="151"><span data-reactid="152"><div data-reactid="153"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="154"><div class=" columns sixteen" data-reactid="155"><div class="s-component s-text" data-reactid="156"><div class="s-component-content s-font-body" data-reactid="157"><p class="s-text-color-blue"><em><strong>Fig.3 The PCR result</strong></em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="158"><span data-reactid="159"><div data-reactid="160"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="161"><div class=" columns sixteen" data-reactid="162"><div class="s-component s-text" data-reactid="163"><div class="s-component-content s-font-body" data-reactid="164"><p class="s-text-color-blue" style="font-size: 130%;">We have really close relationship with Team BGIC China, because we share the same laboratory with them and received training on the cloning cycled together. We also shared a great part of cloning materials in the beginning of our experiment because our materials haven’t arrived yet. We always had great communication about our projects and on our testing experiments, since our projects are based on the same technology platform-cell-free system.</p></div></div></div></div></div></span></div></div></div></div></div></li></ul><!-- react-empty: 165 --><div id="strikingly-tooltip-container" data-reactid="166"> </div><!-- react-empty: 167 --><!-- react-empty: 168 --><div class="s-floated-components" data-reactid="169"><div class="ecommerce-buy-dialog s-edit-modal extend" id="ecommerce-buy-dialog" data-reactid="170"><div class="s-async-wrapper s-component" data-reactid="171"><div class="s-loading" data-reactid="172"></div></div></div><div id="s-ecommerce-shopping-cart-wrapper" class="s-ecommerce-shopping-cart-wrapper" data-reactid="173"></div></div></div></div></div> <div id='fb-root'></div> <div id='app-script-root'></div> <div id='app-view-root'></div> <script id='blog-collection-tmpl' type='text/html'> <div class='s-blog-col-head'></div> <div class='s-blog-col-body'> {{ _.each(blogPosts, function(blog) { }} <div class='s-blog-entry'> <div class='s-blog-entry-inner'> {{ if ($S && $S.conf && $S.conf.preview_mode) { }} <a class='s-blog-overlay-link' href='{{=blog.publicUrl}}'> </a> {{ } else { }} <a class='s-blog-overlay-link' href='{{=blog.relativeUrl}}'> </a> {{ } }} {{ if (blog.iconUrl) { }} <div class='s-blog-entry-left'> {{ if (UrlHelper.imageHasContent(blog.iconUrl)) { }} <div class='s-blog-avatar' style='background-image:url({{=blog.iconUrl}});'></div> {{ } else { }} <div class='s-blog-default-avatar'> <div class='entypo-bookmark'></div> </div> {{ } }} </div> {{ } }} <div class='s-blog-entry-right'> <div class='s-blog-details'> <div class='s-blog-details-head s-blog-row'> <div class='s-blog-title s-font-heading'> {{=blog.title}} </div> <div class='s-blog-date'> {{=blog.publishedAt}} </div> </div> {{ if (blogSettings.previewLayout == 1) { }} <div class='s-blog-details-blurb s-blog-row'> {{=blog.blurb}} </div> {{ } }} </div> </div> </div> </div> {{ }) }} {{ if (blogPosts.length == 0) { }} <div class='s-blog-entry'> <div class='s-common-status no-posts-error'> 没有博文。 </div> </div> {{ } }} </div> {{ if (blogPosts.length > 0) { }} <div class='s-blog-col-foot'> <a class='s-blog-link s-blog-next-link' href='javascript:void(0);'> « 更新 </a> <ul class='s-blog-pagination' style='display:none;'> <li> <a class='s-blog-link s-blog-pagination-page' href='javascript:void(0);'></a> </li> </ul> <a class='s-blog-link s-blog-prev-link' href='javascript:void(0);'> 其它 » </a> </div> {{ } }} </script> <script src="//cdn.bootcss.com/jquery/1.10.0/jquery.min.js"></script> <script> | |
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Latest revision as of 18:08, 18 October 2016
Collaboration
Since SFLS team and us both used the S30 cell-free system in our projects, so we assisted each other on different aspects. Our team helped SFLS team to validate the viability of gene expression in S30 cell-free system. Here is a link to their collaboration page: https://2016.igem.org/Team:Shenzhen_SFLS/Collaborations
Here is an abstract from SFLS team:
Team BGIC China>>Us
Team BGIC China were working on a programmable and open-source Next Generation Bio-Chip (NGBC) which allows user with knowledge in synthetic biology to easily re-program and re-construct it in order to make new bio-chips with different functions. They based their NGBC on E. coli S30 Extract System for Circular DNA(Promega), the same with ours. They also utilized enzyme b-galactosidase (LacZ) as one of their reporter gene. So they helped us to test whether S30 system was qualified as a platform for translation of enzyme b-galactosidase (LacZ). Here is their result.
The blue liquid in the small eppendorf is the S30 system with gene circuit coding enzyme b-galactosidase (LacZ) and its substrates, X-gal, after hours of reaction.
It qualitatively proved that our S30 cell-free system works well.
Fig.1 Test result in S30 cell-free system
We also tested this with a different reporter gene, luciferase. Check our results in the “Proof of Concept” section of our wiki.
SFLS>>Team BGIC China
We helped Team BGIC China by constructing plasmid pfadr for them. Plasmid pfadr is already in the parts collection and is modified to be responsive to fatty acid. Here are some of the results we got in the process.
Fig.2 The Nanodrop results of plasmid extraction
Fig.3 The PCR result
We have really close relationship with Team BGIC China, because we share the same laboratory with them and received training on the cloning cycled together. We also shared a great part of cloning materials in the beginning of our experiment because our materials haven’t arrived yet. We always had great communication about our projects and on our testing experiments, since our projects are based on the same technology platform-cell-free system.
