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<h2> Project Description </h2>
 
<p>As the first high school team of BGI-college, we endeavour to apply the cell-free system to a semi-quantitative test paper with the reaction site in the shape of a pearl-necklace whose results can be observed by human to measure the concentration of target molecules in the sample within a certain range. This mechanism integrated with biosensors for target molecules enabled the testing of the concentration of hormones, glucose, fatty acid or nitrates in clinical samples of plasma, urine or saliva. Quantitative results can be shown by the output of a variety of proteins.(light emission of GFP or the changing of colour initiated by LacZ,etc.). The high portability of the test paper and the small duration of the testing process of clinical samples, should the test paper be successfully manufactured, shall relieve millions of patients of comprehensive time-consuming and perhaps agonising medical inspections—these could be carried out at home! The test paper system, presumably, transcends mainstream medical inspections of clinical samples in means of efficiency and convenience.</p>
 
<p>Our idea originated from comprehensive discussions that eliminated the alternatives of acetochlor test papers which already exists in the market at a feasible price and the cholinesterase injection plan for protection of nerve system against Sarin gas as the project relied upon the injected medicine’s access through the Brain-Blood Barriers——an unsolvable dilemma. We procured research that followed which can be traced all the way back to November 2015. It was by chance that we came across the article Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates written by Alexis Courbet, Drew Endy, Eric Renard, Franck Molina, and Jérôme Bonnet along with Keith Pardee’s articles on paper-based synthetic gene networks. The two articles outlined the feasibility of a paper-based cell-free system for inspection of clinical samples targeting at specific molecules as we grappled what they meant to us, and became the very foundation of our project. In late April, the designing of our plasmids was initiated via comprehensive methods, which, upon finishing even as we post this passage, has gone through three phases. Upon the biosensor’s contact with the target molecule, the genetic circuit will produce an output with the fluorescence of a GFP.</p>
 
<p>Our goals go as follows. The very foundation of our targets is to apply our cell-free system, the system of cell environment simulation which consists of cell-extract and substrate that enables DNA expression in vitro to a mature test paper module. Laboratory sample and clinical sample tests will be carried out to assess the effectiveness of the coalition. To make that happen, we will test the feasibility of our test paper prototype with pearl necklace-shaped reaction region and enhance its operability through modelling until a semi-quantitative test-paper system is built up. Should that succeed, we will expand the project into the designing, testing and, if possible, manufacturing of a test paper reader: To assemble a test paper reader according to existing blueprints and express the measurements via reporter proteins from single apertures. </p>
 
  
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        color: #363636;}.s-custom-colors .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-cards .s-ecommerce-card-view-card .s-ecommerce-card-view-card-price{color: #363636;}.s-custom-colors .s-ecommerce .s-ecommerce-row-view-product .s-ecommerce-row-view-product-detail-panel .s-ecommerce-row-view-product-pricing{color: #363636;}.s-custom-colors .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .next-product-btn{color: #363636;}.s-custom-colors .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .prev-product-btn{color: #363636;}.s-custom-colors .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .back-btn{color: #363636;}.s-custom-colors .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .next-product-btn:hover{color: #5e5e5e;}.s-custom-colors .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .prev-product-btn:hover{color: #5e5e5e;}.s-custom-colors .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .back-btn:hover{color: #5e5e5e;}
 +
.s-custom-colors #header-container .nav-item a:hover,
 +
.s-custom-colors #header-container .nav-item a.selected {
 +
  color: #040404;
 +
  border-bottom: 1px solid #040404;
 +
}
 +
.s-custom-colors .s-section.s-bright-alt-dark {
 +
  background-color: #2e2f32;
 +
}
 +
.s-custom-colors .s-section.s-bright-alt {
 +
  background-color: #c0c0c0;
 +
}
 +
.s-custom-colors .s-section.s-info-section .s-info-box .s-item-subtitle,
 +
.s-custom-colors .s-section.s-rows-section .s-item-text-group .s-item-subtitle,
 +
.s-custom-colors .s-section.s-media-section .s-layout-mediaLeft .s-item-text-group .s-item-subtitle,
 +
.s-custom-colors .s-section.s-media-section .s-layout-mediaRight .s-item-text-group .s-item-subtitle,
 +
.s-custom-colors .s-section.s-media-section .s-layout-alt .s-item-text-group .s-item-subtitle,
 +
.s-custom-colors .s-section.s-columns-section .s-item-text-group .s-item-subtitle,
 +
.s-custom-colors .s-section.s-block-section .s-block-feature .s-item-text-group .s-item-subtitle,
 +
.s-custom-colors .s-section.s-block-section .s-block-profile .s-item-text-group .s-item-subtitle {
 +
  color: #040404;
 +
}
  
 +
.s-custom-colors .s-section.s-info-section .s-info-box .s-item-subtitle .s-text .s-component-content a,
 +
.s-custom-colors .s-section.s-rows-section .s-item-text-group .s-item-subtitle .s-text .s-component-content a,
 +
.s-custom-colors .s-section.s-media-section .s-layout-mediaLeft .s-item-text-group .s-item-subtitle .s-text .s-component-content a,
 +
.s-custom-colors .s-section.s-media-section .s-layout-mediaRight .s-item-text-group .s-item-subtitle .s-text .s-component-content a,
 +
.s-custom-colors .s-section.s-media-section .s-layout-alt .s-item-text-group .s-item-subtitle .s-text .s-component-content a,
 +
.s-custom-colors .s-section.s-columns-section .s-item-text-group .s-item-subtitle .s-text .s-component-content a,
 +
.s-custom-colors .s-section.s-block-section .s-block-feature .s-item-text-group .s-item-subtitle .s-text .s-component-content a,
 +
.s-custom-colors .s-section.s-block-section .s-block-profile .s-item-text-group .s-item-subtitle .s-text .s-component-content a {
 +
  color: #040404;
 +
  border-bottom: 1px solid #040404;
 +
}
  
<div class="column half_size" >
+
@media only screen and (max-width: 727px) {
<h5>Before you start: </h5>
+
  .s-custom-colors .navbar-drawer-bar a {
<p> Please read the following pages:</p>
+
    background: #040404;
<ul>
+
  }
<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
+
}.s-custom-colors .s-bright-alt-dark .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-cards .s-ecommerce-card-view-card .s-ecommerce-card-view-card-price{color: #5b5b5b;}.s-custom-colors .s-bright-alt-dark .s-ecommerce .s-ecommerce-row-view-product .s-ecommerce-row-view-product-detail-panel .s-ecommerce-row-view-product-pricing{color: #5b5b5b;}.s-custom-colors .s-bright-alt-dark .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .next-product-btn{color: #5b5b5b;}.s-custom-colors .s-bright-alt-dark .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .prev-product-btn{color: #5b5b5b;}.s-custom-colors .s-bright-alt-dark .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .back-btn{color: #5b5b5b;}.s-custom-colors .s-bright-alt-dark .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .next-product-btn:hover{color: #7b7b7b;}.s-custom-colors .s-bright-alt-dark .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .prev-product-btn:hover{color: #7b7b7b;}.s-custom-colors .s-bright-alt-dark .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .back-btn:hover{color: #7b7b7b;}
<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
+
.s-custom-colors .s-section.s-bright-alt-dark .s-blog-wrapper .s-blog-col-placeholder .s-blog-details .s-blog-info {
</ul>
+
  color: #5b5b5b;
</div>
+
}.s-custom-colors .s-bright-alt .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-cards .s-ecommerce-card-view-card .s-ecommerce-card-view-card-price{color: #ffffff;}.s-custom-colors .s-bright-alt .s-ecommerce .s-ecommerce-row-view-product .s-ecommerce-row-view-product-detail-panel .s-ecommerce-row-view-product-pricing{color: #ffffff;}.s-custom-colors .s-bright-alt .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .next-product-btn{color: #ffffff;}.s-custom-colors .s-bright-alt .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .prev-product-btn{color: #ffffff;}.s-custom-colors .s-bright-alt .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .back-btn{color: #ffffff;}.s-custom-colors .s-bright-alt .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .next-product-btn:hover{color: #ffffff;}.s-custom-colors .s-bright-alt .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .prev-product-btn:hover{color: #ffffff;}.s-custom-colors .s-bright-alt .s-ecommerce .s-ecommerce-products-wrapper .s-ecommerce-card-view-wrapper .s-ecommerce-card-view-detail .s-ecommerce-card-view-detail-header .back-btn:hover{color: #ffffff;}
 
+
.s-custom-colors .s-section.s-bright-alt .s-blog-wrapper .s-blog-col-placeholder .s-blog-details .s-blog-info {
<div class="column half_size" >
+
  color: white;
<div class="highlight">
+
}.s-custom-colors .s-text-color-custom1{ color: #040404; }
<h5> Styling your wiki </h5>
+
.s-custom-colors .s-text-color-custom2{ color: #c0c0c0; }
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
</style><div class="s-navbar-section" data-reactid="12"><div id="header-container" class="navigator " data-reactid="13"><div class="nav-left" data-reactid="14"><span class="nav-items" data-reactid="15"></span></div><div class="nav-center" data-reactid="16"><div class="logo" data-reactid="17"><div class="s-component s-image" data-reactid="18"><span data-reactid="19"><div class="s-component-content" data-reactid="20"><div data-reactid="21"><!-- react-text: 22 --> <!-- /react-text --><img src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto,q_90/v1/791914/Team_logo_final_%E5%89%AF%E6%9C%AC_%E5%89%AF%E6%9C%AC_ffweyb.png" alt="" title="" data-description="" data-reactid="23"/><!-- react-text: 24 --> <!-- /react-text --></div></div></span></div></div></div><div class="nav-right" data-reactid="25"><span class="nav-items" data-reactid="26"></span></div><div class="nav original-nav" data-reactid="27"><span data-reactid="28"><span class="nav-item" style="display:none;" data-reactid="29"><a class="s-nav-item" href="#beam" data-reactid="30"><span class="s-font-body" data-reactid="31">Title</span></a></span><span class="nav-item" style="display:none;" data-reactid="32"><a class="s-nav-item" href="#what-is-beam" data-reactid="33"><span class="s-font-body" data-reactid="34">Slide</span></a></span><span class="nav-item" style="display:none;" data-reactid="35"><a class="s-nav-item" href="#beam-projects" data-reactid="36"><span class="s-font-body" data-reactid="37">Project Abstract</span></a></span><span class="nav-item" style="display:none;" data-reactid="38"><a class="s-nav-item" href="#arbeit-4-5" data-reactid="39"><span class="s-font-body" data-reactid="40">Other parts</span></a></span><span class="nav-item" style="display:none;" data-reactid="41"><a class="s-nav-item" href="#how-dare-you-video-blog" data-reactid="42"><span class="s-font-body" data-reactid="43">Sponsor</span></a></span></span><span data-reactid="44"><li data-reactid="45"><a class="s-external-link-item s-nav-item" href="https://2016.igem.org/Team:BGIC_China/Description" target="_blank" data-reactid="46"><span class="s-font-body" data-reactid="47">Project description</span></a></li><li data-reactid="48"><a class="s-external-link-item s-nav-item" href="https://2016.igem.org/Team:BGIC_China/Proof" target="_blank" data-reactid="49"><span class="s-font-body" data-reactid="50">Proof of concept</span></a></li><li data-reactid="51"><a class="s-external-link-item s-nav-item" href="https://2016.igem.org/Team:BGIC_China/Parts" target="_blank" data-reactid="52"><span class="s-font-body" data-reactid="53">Parts</span></a></li><li data-reactid="54"><a class="s-external-link-item s-nav-item" href="https://2016.igem.org/Team:BGIC_China/HP/Gold" target="_blank" data-reactid="55"><span class="s-font-body" data-reactid="56">Human practice</span></a></li><li data-reactid="57"><a class="s-external-link-item s-nav-item" href="https://2016.igem.org/Team:BGIC_China/Attributions" target="_blank" data-reactid="58"><span class="s-font-body" data-reactid="59">Attributions</span></a></li><li data-reactid="60"><a class="s-external-link-item s-nav-item" href="https://2016.igem.org/Team:BGIC_China/Collaborations" target="_blank" data-reactid="61"><span class="s-font-body" data-reactid="62">Collaborations</span></a></li></span></div></div><div id="header-container-fixed" class="navigator strikingly-fixed" data-reactid="63"><div class="nav-left" data-reactid="64"><span class="nav-items" data-reactid="65"></span></div><div class="nav-center" data-reactid="66"><div class="logo" data-reactid="67"><div class="s-component s-image" data-reactid="68"><span data-reactid="69"><div class="s-component-content" data-reactid="70"><div data-reactid="71"><!-- react-text: 72 --> <!-- /react-text --><img src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto,q_90/v1/791914/Team_logo_final_%E5%89%AF%E6%9C%AC_%E5%89%AF%E6%9C%AC_ffweyb.png" alt="" title="" data-description="" data-reactid="73"/><!-- react-text: 74 --> <!-- /react-text --></div></div></span></div></div></div><div class="nav-right" data-reactid="75"><span class="nav-items" data-reactid="76"></span></div></div></div><!-- react-empty: 77 --><ul class="slides s-page-1" data-reactid="78"><li class="slide s-section-1" id="section-f_2689c0d2-ada7-41e9-97eb-0be2cf91b15f" data-reactid="79"><div class="waypoint" data-reactid="80"></div><a class="section-anchor" data-reactid="81"></a><div class="s-bg-image s-bg-light-text s-bg-overlay s-cta-section s-section" style="background-repeat:no-repeat;background-size:cover;background-color:transparent;background-position:50% 50%;background-image:url(//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1500,w_2000,f_auto,q_90/v1/791914/pic1_mntejh.jpg);" data-reactid="82"><div class="container s-layout-mediaTop" data-reactid="83"><div class="columns sixteen" data-reactid="84"><div class="s-title-group " data-reactid="85"><div class="s-title " data-reactid="86"><div class="s-component s-text" data-reactid="87"><h1 class="s-component-content s-font-title" data-reactid="88"><p style="font-size:80%" class="s-text-color-custom2">Paper, but more than a paper</p></h1></div></div><div class="s-subtitle" data-reactid="89"><div class="s-component s-text" data-reactid="90"><h4 class="s-component-content s-font-heading" data-reactid="91"><p class="s-text-color-custom2">Programmable and open-source Next Generation Bio-Chip (NGBC) based on cell-free system</p></h4></div></div></div><div class="s-button-group " data-reactid="92"><div class="s-button s-component" data-reactid="93"><span data-reactid="94"><div class="s-component-content" data-reactid="95"><a class="s-action-button s-common-button s-font-body" href="#2" data-component="button" target="_self" data-reactid="96">READ MORE ↓</a></div></span></div></div></div></div></div></li><li class="slide s-section-2" id="section-f_16951574-e142-4b54-86c5-19cba5f9cb49" data-reactid="97"><div class="waypoint" data-reactid="98"></div><a class="section-anchor" data-reactid="99"></a><div class="s-section s-slider-section" data-reactid="100"><div class="s-component s-slider " data-reactid="101"><!-- react-text: 102 --> <!-- /react-text --><!-- react-text: 103 --> <!-- /react-text --><div class="s-component-content" data-reactid="104"><div class="slider-container loading" data-reactid="105"><div data-auto-play="3500" class="iosslider" data-reactid="106"><div class="slider" data-reactid="107"><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay item" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/49.jpg" data-reactid="108"><div class="inner" data-reactid="109"><div class="container right s-rva" data-reactid="110"><div class="eight columns right media-outer s-rva-media" data-reactid="111"><div class="media-wrapper" data-reactid="112"><div class="s-component s-media " data-reactid="113"><span data-reactid="114"><div class="s-component-content" data-reactid="115"><div data-reactid="116"><!-- react-text: 117 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:200px;max-height:200px;" data-reactid="118"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="119"></div><div data-reactid="120"><img alt="" title="" data-description="" width="200" height="200" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/programmable_200_pajmy7.png" data-sizes="auto" data-reactid="121"/><noscript data-reactid="122"><img alt="" title="" data-description="" width="200" height="200" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/programmable_200_pajmy7.png" class="" data-reactid="123"/></noscript></div></div><!-- react-text: 124 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="125"><div class="half-offset-right" data-reactid="126"><div class="s-title-group " data-reactid="127"><div class="s-title " data-reactid="128"><div class="s-component s-text" data-reactid="129"><h2 class="s-component-content s-font-title" data-reactid="130"><p class="s-text-color-custom2">Programmable</p></h2></div></div><div class="s-subtitle" data-reactid="131"><div class="s-component s-text" data-reactid="132"><h4 class="s-component-content s-font-heading" data-reactid="133"><p class="s-text-color-custom2">Give bio-chip different functions by re-construct immobilized genetic circuits.</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="134"><div class="" data-reactid="135"><div class="s-button s-component" data-reactid="136"><span data-reactid="137"><div class="s-component-content" data-reactid="138"></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay item" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/49.jpg" data-reactid="139"><div class="inner" data-reactid="140"><div class="container right s-rva" data-reactid="141"><div class="eight columns right media-outer s-rva-media" data-reactid="142"><div class="media-wrapper" data-reactid="143"><div class="s-component s-media " data-reactid="144"><span data-reactid="145"><div class="s-component-content" data-reactid="146"><div data-reactid="147"><!-- react-text: 148 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:200px;max-height:200px;" data-reactid="149"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="150"></div><div data-reactid="151"><img alt="" title="" data-description="" width="200" height="200" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/Cell-free_iia73w.png" data-sizes="auto" data-reactid="152"/><noscript data-reactid="153"><img alt="" title="" data-description="" width="200" height="200" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/Cell-free_iia73w.png" class="" data-reactid="154"/></noscript></div></div><!-- react-text: 155 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="156"><div class="half-offset-right" data-reactid="157"><div class="s-title-group " data-reactid="158"><div class="s-title " data-reactid="159"><div class="s-component s-text" data-reactid="160"><h2 class="s-component-content s-font-title" data-reactid="161"><p class="s-text-color-custom2">Cell-Free</p></h2></div></div><div class="s-subtitle" data-reactid="162"><div class="s-component s-text" data-reactid="163"><h4 class="s-component-content s-font-heading" data-reactid="164"><p class="s-text-color-custom2">Bio-safety, low noise and more possibilities.</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="165"><div class="" data-reactid="166"><div class="s-button s-component" data-reactid="167"><span data-reactid="168"><div class="s-component-content" data-reactid="169"></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay item" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/49.jpg" data-reactid="170"><div class="inner" data-reactid="171"><div class="container right s-rva" data-reactid="172"><div class="eight columns right media-outer s-rva-media" data-reactid="173"><div class="media-wrapper" data-reactid="174"><div class="s-component s-media " data-reactid="175"><span data-reactid="176"><div class="s-component-content" data-reactid="177"><div data-reactid="178"><!-- react-text: 179 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:200px;max-height:200px;" data-reactid="180"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="181"></div><div data-reactid="182"><img alt="" title="" data-description="" width="200" height="200" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/open_source_woezqa.png" data-sizes="auto" data-reactid="183"/><noscript data-reactid="184"><img alt="" title="" data-description="" width="200" height="200" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/open_source_woezqa.png" class="" data-reactid="185"/></noscript></div></div><!-- react-text: 186 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="187"><div class="half-offset-right" data-reactid="188"><div class="s-title-group " data-reactid="189"><div class="s-title " data-reactid="190"><div class="s-component s-text" data-reactid="191"><h2 class="s-component-content s-font-title" data-reactid="192"><p class="s-text-color-custom2">Open-Source</p></h2></div></div><div class="s-subtitle" data-reactid="193"><div class="s-component s-text" data-reactid="194"><h4 class="s-component-content s-font-heading" data-reactid="195"><p class="s-text-color-custom2">Distribute technology around the world.</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="196"><div class="" data-reactid="197"><div class="s-button s-component" data-reactid="198"><span data-reactid="199"><div class="s-component-content" data-reactid="200"></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay item" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/49.jpg" data-reactid="201"><div class="inner" data-reactid="202"><div class="container right s-rva" data-reactid="203"><div class="eight columns right media-outer s-rva-media" data-reactid="204"><div class="media-wrapper" data-reactid="205"><div class="s-component s-media " data-reactid="206"><span data-reactid="207"><div class="s-component-content" data-reactid="208"><div data-reactid="209"><!-- react-text: 210 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:200px;max-height:200px;" data-reactid="211"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="212"></div><div data-reactid="213"><img alt="" title="" data-description="" width="200" height="200" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/short_prototyping_time_ijzly8.png" data-sizes="auto" data-reactid="214"/><noscript data-reactid="215"><img alt="" title="" data-description="" width="200" height="200" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/short_prototyping_time_ijzly8.png" class="" data-reactid="216"/></noscript></div></div><!-- react-text: 217 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="218"><div class="half-offset-right" data-reactid="219"><div class="s-title-group " data-reactid="220"><div class="s-title " data-reactid="221"><div class="s-component s-text" data-reactid="222"><h2 class="s-component-content s-font-title" data-reactid="223"><p class="s-text-color-custom2">Short Prototyping Time</p></h2></div></div><div class="s-subtitle" data-reactid="224"><div class="s-component s-text" data-reactid="225"><h4 class="s-component-content s-font-heading" data-reactid="226"><p class="s-text-color-custom2">Build a new bio-chip just with in a week.</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="227"><div class="" data-reactid="228"><div class="s-button s-component" data-reactid="229"><span data-reactid="230"><div class="s-component-content" data-reactid="231"></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay item" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/49.jpg" data-reactid="232"><div class="inner" data-reactid="233"><div class="container right s-rva" data-reactid="234"><div class="eight columns right media-outer s-rva-media" data-reactid="235"><div class="media-wrapper" data-reactid="236"><div class="s-component s-media " data-reactid="237"><span data-reactid="238"><div class="s-component-content" data-reactid="239"><div data-reactid="240"><!-- react-text: 241 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:200px;max-height:200px;" data-reactid="242"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="243"></div><div data-reactid="244"><img alt="" title="" data-description="" width="200" height="200" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/963306978_%E5%89%AF%E6%9C%AC_oththa.png" data-sizes="auto" data-reactid="245"/><noscript data-reactid="246"><img alt="" title="" data-description="" width="200" height="200" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/963306978_%E5%89%AF%E6%9C%AC_oththa.png" class="" data-reactid="247"/></noscript></div></div><!-- react-text: 248 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="249"><div class="half-offset-right" data-reactid="250"><div class="s-title-group " data-reactid="251"><div class="s-title " data-reactid="252"><div class="s-component s-text" data-reactid="253"><h2 class="s-component-content s-font-title" data-reactid="254"><p class="s-text-color-custom2">One-Step &amp; High-Throughput</p></h2></div></div><div class="s-subtitle" data-reactid="255"><div class="s-component s-text" data-reactid="256"><h4 class="s-component-content s-font-heading" data-reactid="257"><p class="s-text-color-custom2">Complete all the reactions on one bio-chip and test for multiple samples together.</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="258"><div class="" data-reactid="259"><div class="s-button s-component" data-reactid="260"><span data-reactid="261"><div class="s-component-content" data-reactid="262"></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay item" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/49.jpg" data-reactid="263"><div class="inner" data-reactid="264"><div class="container right s-rva" data-reactid="265"><div class="eight columns right media-outer s-rva-media" data-reactid="266"><div class="media-wrapper" data-reactid="267"><div class="s-component s-media " data-reactid="268"><span data-reactid="269"><div class="s-component-content" data-reactid="270"><div data-reactid="271"><!-- react-text: 272 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:200px;max-height:200px;" data-reactid="273"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="274"></div><div data-reactid="275"><img alt="" title="" data-description="" width="200" height="200" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/Ultra-high_expression_htz7ij.png" data-sizes="auto" data-reactid="276"/><noscript data-reactid="277"><img alt="" title="" data-description="" width="200" height="200" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/Ultra-high_expression_htz7ij.png" class="" data-reactid="278"/></noscript></div></div><!-- react-text: 279 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="280"><div class="half-offset-right" data-reactid="281"><div class="s-title-group " data-reactid="282"><div class="s-title " data-reactid="283"><div class="s-component s-text" data-reactid="284"><h2 class="s-component-content s-font-title" data-reactid="285"><p class="s-text-color-custom2">Ultra-High Expression</p></h2></div></div><div class="s-subtitle" data-reactid="286"><div class="s-component s-text" data-reactid="287"><h4 class="s-component-content s-font-heading" data-reactid="288"><p class="s-text-color-custom2">U-HEC (Utra-High Expression Cassette) enables high protein expression on the bio-chip.</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="289"><div class="" data-reactid="290"><div class="s-button s-component" data-reactid="291"><span data-reactid="292"><div class="s-component-content" data-reactid="293"></div></span></div></div></div></div></div></div></div></div><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay item" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/49.jpg" data-reactid="294"><div class="inner" data-reactid="295"><div class="container right s-rva" data-reactid="296"><div class="eight columns right media-outer s-rva-media" data-reactid="297"><div class="media-wrapper" data-reactid="298"><div class="s-component s-media " data-reactid="299"><span data-reactid="300"><div class="s-component-content" data-reactid="301"><div data-reactid="302"><!-- react-text: 303 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:200px;max-height:200px;" data-reactid="304"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="305"></div><div data-reactid="306"><img alt="" title="" data-description="" width="200" height="200" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/unlimited_potentials_eqmpdt.png" data-sizes="auto" data-reactid="307"/><noscript data-reactid="308"><img alt="" title="" data-description="" width="200" height="200" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_1440,w_720,f_auto/v1/791914/unlimited_potentials_eqmpdt.png" class="" data-reactid="309"/></noscript></div></div><!-- react-text: 310 --> <!-- /react-text --></div></div></span></div></div></div><div class="columns right left-align valign slider-desc s-rva-text eight" data-reactid="311"><div class="half-offset-right" data-reactid="312"><div class="s-title-group " data-reactid="313"><div class="s-title " data-reactid="314"><div class="s-component s-text" data-reactid="315"><h2 class="s-component-content s-font-title" data-reactid="316"><p class="s-text-color-custom2">Unlimited Potentials</p></h2></div></div><div class="s-subtitle" data-reactid="317"><div class="s-component s-text" data-reactid="318"><h4 class="s-component-content s-font-heading" data-reactid="319"><p class="s-text-color-custom2">It is possible to testing for everything by construct proper genetic circuits.</p></h4></div></div></div><div class="cta-button-wrapper" data-reactid="320"><div class="" data-reactid="321"><div class="s-button s-component" data-reactid="322"><span data-reactid="323"><div class="s-component-content" data-reactid="324"></div></span></div></div></div></div></div></div></div></div></div><div class="center slide-selectors" data-reactid="325"><div class="slide-selectors-inner" data-reactid="326"><div class="content repeatable-component" data-reactid="327"><div class="selector-wrapper" data-reactid="328"><div class="selector" data-reactid="329"></div></div><div class="selector-wrapper" data-reactid="330"><div class="selector" data-reactid="331"></div></div><div class="selector-wrapper" data-reactid="332"><div class="selector" data-reactid="333"></div></div><div class="selector-wrapper" data-reactid="334"><div class="selector" data-reactid="335"></div></div><div class="selector-wrapper" data-reactid="336"><div class="selector" data-reactid="337"></div></div><div class="selector-wrapper" data-reactid="338"><div class="selector" data-reactid="339"></div></div><div class="selector-wrapper" data-reactid="340"><div class="selector" data-reactid="341"></div></div><div class="clearfix" data-reactid="342"></div></div></div></div><div class="prev-button" data-reactid="343"><div class="arrow" data-reactid="344"></div></div><div class="next-button" data-reactid="345"><div class="arrow" data-reactid="346"></div></div></div></div></div></div></div></li><li class="slide s-section-3" id="section-f_498d3f75-a829-4591-b39c-655949bf5eda" data-reactid="347"><div class="waypoint" data-reactid="348"></div><a class="section-anchor" data-reactid="349"></a><div class="lazyload s-no-bg s-block-section s-section" data-reactid="350"><div class="container" data-reactid="351"><div class="columns sixteen" data-reactid="352"><div class="s-title-group " data-reactid="353"><div class="s-title " data-reactid="354"><div class="s-component s-text" data-reactid="355"><h2 class="s-component-content s-font-title" data-reactid="356"><p>Project Abstract</p></h2></div></div></div></div><div class="alignment-container " data-reactid="357"><div class="s-repeatable s-block s-component s-mh " data-reactid="358"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="359"><span data-reactid="360"><div data-reactid="361"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="362"><div class=" columns sixteen" data-reactid="363"><div class="s-component s-text" data-reactid="364"><div class="s-component-content s-font-body" data-reactid="365"><p style="font-size:130%; text-align:left">NGBC --- Next Generation Bio-Chip, is what we are going to create. This will be the first bio-chip ever that allows user with knowledge in synthetic biology to easily re-program and re-construct it in order to make new bio-chips with different functions.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="366"><span data-reactid="367"><div data-reactid="368"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="369"><div class=" columns sixteen" data-reactid="370"><div class="s-component s-text" data-reactid="371"><div class="s-component-content s-font-body" data-reactid="372"><p style="font-size:130%; text-align:left">NGBC use genetic circuits to process the input signals and given output results. In order to minimize the disruptions and limitations of biochemical reactions inside a living-cell, the reaction center of NGBC will use S30 cell-free system which is produced from cell extracts and could provide all the conditions required for biochemical reactions like that inside a real living-cell. What is most amazing is that the complete genetic circuits and cell-free system is just carried by simple filter paper and no other expensive materials required to construct it. We are also developing a hardware and a software which can be integrated with NGBC to provide a more accurate data readout and a more interactive user environment.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="373"><span data-reactid="374"><div data-reactid="375"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="376"><div class=" columns sixteen" data-reactid="377"><div class="s-component s-text" data-reactid="378"><div class="s-component-content s-font-body" data-reactid="379"><p style="font-size:130%; text-align:left">In future, we intended to develop a completely open-source NGBC kit which contains all the necessary materials, protocols and genetic components required to re-program the BioChip, and the kit will be distributed to labs around the world so there will be more new BioChips with different functions be developed. We will also create an NGBC clinical database that includes all the verified BioChips which are able to measure the concentration of target clinical molecules such as glucose, nitrate and fatty-acids in the clinical sample with in a certain range.</p></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide s-section-4" id="section-f_dc10117f-1cc6-41a0-9381-17b786992ba0" data-reactid="380"><div class="waypoint" data-reactid="381"></div><a class="section-anchor" data-reactid="382"></a><div class="lazyload s-bg-image s-bg-light-text s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/34.jpg" data-reactid="383"><div class="container" data-reactid="384"><div class="columns sixteen" data-reactid="385"><!-- react-empty: 386 --></div><div class="alignment-container " data-reactid="387"><div class="s-repeatable s-block s-component s-mh " data-reactid="388"><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-smallFeatureLeft " data-reactid="389"><span data-reactid="390"><div data-reactid="391"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="392"><div class="" data-reactid="393"><div class="s-block-feature " data-reactid="394"><div class="six columns" data-reactid="395"><div class="s-item-media-group" data-reactid="396"><div class="s-component s-media " data-reactid="397"><span data-reactid="398"><div class="s-component-content" data-reactid="399"><div data-reactid="400"><a href="https://2016.igem.org/Team:BGIC_China/Description" target="_blank" data-reactid="401"><div class="s-ratio-box" style="max-width:160px;max-height:160px;" data-reactid="402"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="403"></div><div data-reactid="404"><img alt="" title="" data-description="" width="160" height="160" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//uploads.strikinglycdn.com/static/icons/line-icons/8.png" data-sizes="auto" data-reactid="405"/><noscript data-reactid="406"><img alt="" title="" data-description="" width="160" height="160" src="//uploads.strikinglycdn.com/static/icons/line-icons/8.png" class="" data-reactid="407"/></noscript></div></div></a><!-- react-text: 408 --> <!-- /react-text --><!-- react-text: 409 --> <!-- /react-text --></div></div></span></div></div></div><div class="ten columns" data-reactid="410"><div class="s-item-text-group half-offset-left" data-reactid="411"><div class="s-item-title" data-reactid="412"><div class="s-component s-text" data-reactid="413"><h3 class="s-component-content s-font-heading" data-reactid="414"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Description"><strong>Project description</strong></a></p></h3></div></div><div class="s-item-subtitle" data-reactid="415"><div class="s-component s-text" data-reactid="416"><h6 class="s-component-content s-font-body" data-reactid="417"><p>We are going to design the first programmable BioChip ever by using genetic boolean logic gates(Piro et al, 2013) which enable the BioChip to achieve complex tasks. Several other amazing features like cell-free expression system and ultra-high expression cassette(U-HEC) are also introduced...</p><p>(This part includes <em><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Model">Mathematical Modelling</a></em> and <em><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Demonstrate">Demonstration</a></em>)</p></h6></div></div><div class="s-item-text" data-reactid="418"><div class="s-component s-text" data-reactid="419"><div class="s-component-content s-font-body" data-reactid="420"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Description"><strong>READ MORE</strong></a></p></div></div></div></div></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-smallFeatureRight " data-reactid="421"><span data-reactid="422"><div data-reactid="423"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="424"><div class="" data-reactid="425"><div class="s-block-feature " data-reactid="426"><div class="six columns right" data-reactid="427"><div class="s-item-media-group" data-reactid="428"><div class="s-component s-media " data-reactid="429"><span data-reactid="430"><div class="s-component-content" data-reactid="431"><div data-reactid="432"><a href="https://2016.igem.org/Team:BGIC_China/Proof" target="_blank" data-reactid="433"><div class="s-ratio-box" style="max-width:160px;max-height:160px;" data-reactid="434"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="435"></div><div data-reactid="436"><img alt="" title="" data-description="" width="160" height="160" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//uploads.strikinglycdn.com/static/icons/line-icons/49.png" data-sizes="auto" data-reactid="437"/><noscript data-reactid="438"><img alt="" title="" data-description="" width="160" height="160" src="//uploads.strikinglycdn.com/static/icons/line-icons/49.png" class="" data-reactid="439"/></noscript></div></div></a><!-- react-text: 440 --> <!-- /react-text --><!-- react-text: 441 --> <!-- /react-text --></div></div></span></div></div></div><div class="ten columns right" data-reactid="442"><div class="s-item-text-group half-offset-right" data-reactid="443"><div class="s-item-title" data-reactid="444"><div class="s-component s-text" data-reactid="445"><h3 class="s-component-content s-font-heading" data-reactid="446"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Proof"><strong>Proof of concept</strong></a></p></h3></div></div><div class="s-item-subtitle" data-reactid="447"><div class="s-component s-text" data-reactid="448"><h6 class="s-component-content s-font-body" data-reactid="449"><p>In order to prove the feasibility of our idea, we constructed a simple plasmid with a pH inducible promoter with EGFP coding sequence in the downstream of the promoter. We immobilized this plasmid onto our bio-chip system and tested for the RFU value in comparison with a standard constitutive J23119 promoter ...</p></h6></div></div><div class="s-item-text" data-reactid="450"><div class="s-component s-text" data-reactid="451"><div class="s-component-content s-font-body" data-reactid="452"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Proof"><strong>READ MORE</strong></a></p></div></div></div></div></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-smallFeatureLeft " data-reactid="453"><span data-reactid="454"><div data-reactid="455"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="456"><div class="" data-reactid="457"><div class="s-block-feature " data-reactid="458"><div class="six columns" data-reactid="459"><div class="s-item-media-group" data-reactid="460"><div class="s-component s-media " data-reactid="461"><span data-reactid="462"><div class="s-component-content" data-reactid="463"><div data-reactid="464"><a href="https://2016.igem.org/Team:BGIC_China/Parts" target="_blank" data-reactid="465"><div class="s-ratio-box" style="max-width:160px;max-height:160px;" data-reactid="466"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="467"></div><div data-reactid="468"><img alt="" title="" data-description="" width="160" height="160" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//uploads.strikinglycdn.com/static/icons/line-icons/33.png" data-sizes="auto" data-reactid="469"/><noscript data-reactid="470"><img alt="" title="" data-description="" width="160" height="160" src="//uploads.strikinglycdn.com/static/icons/line-icons/33.png" class="" data-reactid="471"/></noscript></div></div></a><!-- react-text: 472 --> <!-- /react-text --><!-- react-text: 473 --> <!-- /react-text --></div></div></span></div></div></div><div class="ten columns" data-reactid="474"><div class="s-item-text-group half-offset-left" data-reactid="475"><div class="s-item-title" data-reactid="476"><div class="s-component s-text" data-reactid="477"><h3 class="s-component-content s-font-heading" data-reactid="478"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Parts"><strong>Parts</strong></a></p></h3></div></div><div class="s-item-subtitle" data-reactid="479"><div class="s-component s-text" data-reactid="480"><h6 class="s-component-content s-font-body" data-reactid="481"><p>BBa_K2026000 --- a composite part with two B0015 sequence flanked by two same directional loxp sites.</p><p>BBa_K2026001 --- pH and glucose inducible ultra-strong promoter from Cpx regulon.</p></h6></div></div><div class="s-item-text" data-reactid="482"><div class="s-component s-text" data-reactid="483"><div class="s-component-content s-font-body" data-reactid="484"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Parts"><strong>READ MORE</strong></a></p></div></div></div></div></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-smallFeatureRight " data-reactid="485"><span data-reactid="486"><div data-reactid="487"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="488"><div class="" data-reactid="489"><div class="s-block-feature " data-reactid="490"><div class="six columns right" data-reactid="491"><div class="s-item-media-group" data-reactid="492"><div class="s-component s-media " data-reactid="493"><span data-reactid="494"><div class="s-component-content" data-reactid="495"><div data-reactid="496"><a href="https://2016.igem.org/Team:BGIC_China/HP/Gold" target="_blank" data-reactid="497"><div class="s-ratio-box" style="max-width:160px;max-height:160px;" data-reactid="498"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="499"></div><div data-reactid="500"><img alt="" title="" data-description="" width="160" height="160" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//uploads.strikinglycdn.com/static/icons/line-icons/15.png" data-sizes="auto" data-reactid="501"/><noscript data-reactid="502"><img alt="" title="" data-description="" width="160" height="160" src="//uploads.strikinglycdn.com/static/icons/line-icons/15.png" class="" data-reactid="503"/></noscript></div></div></a><!-- react-text: 504 --> <!-- /react-text --><!-- react-text: 505 --> <!-- /react-text --></div></div></span></div></div></div><div class="ten columns right" data-reactid="506"><div class="s-item-text-group half-offset-right" data-reactid="507"><div class="s-item-title" data-reactid="508"><div class="s-component s-text" data-reactid="509"><h3 class="s-component-content s-font-heading" data-reactid="510"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/HP/Gold"><strong>Human practice</strong></a></p></h3></div></div><div class="s-item-subtitle" data-reactid="511"><div class="s-component s-text" data-reactid="512"><h6 class="s-component-content s-font-body" data-reactid="513"><p>We introduced our project to visitors in London International Youth Science Forum (LIYSF) and spread the concept of synthetic biology including bio-safety to high school students and Bio-Maker organisations...</p></h6></div></div><div class="s-item-text" data-reactid="514"><div class="s-component s-text" data-reactid="515"><div class="s-component-content s-font-body" data-reactid="516"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/HP/Gold"><strong>READ MORE</strong></a></p></div></div></div></div></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-smallFeatureLeft " data-reactid="517"><span data-reactid="518"><div data-reactid="519"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="520"><div class="" data-reactid="521"><div class="s-block-feature " data-reactid="522"><div class="six columns" data-reactid="523"><div class="s-item-media-group" data-reactid="524"><div class="s-component s-media " data-reactid="525"><span data-reactid="526"><div class="s-component-content" data-reactid="527"><div data-reactid="528"><a href="https://2016.igem.org/Team:BGIC_China/Attributions" target="_blank" data-reactid="529"><div class="s-ratio-box" style="max-width:160px;max-height:160px;" data-reactid="530"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="531"></div><div data-reactid="532"><img alt="" title="" data-description="" width="160" height="160" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//uploads.strikinglycdn.com/static/icons/line-icons/93.png" data-sizes="auto" data-reactid="533"/><noscript data-reactid="534"><img alt="" title="" data-description="" width="160" height="160" src="//uploads.strikinglycdn.com/static/icons/line-icons/93.png" class="" data-reactid="535"/></noscript></div></div></a><!-- react-text: 536 --> <!-- /react-text --><!-- react-text: 537 --> <!-- /react-text --></div></div></span></div></div></div><div class="ten columns" data-reactid="538"><div class="s-item-text-group half-offset-left" data-reactid="539"><div class="s-item-title" data-reactid="540"><div class="s-component s-text" data-reactid="541"><h3 class="s-component-content s-font-heading" data-reactid="542"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Attributions"><strong>Attributions</strong></a></p></h3></div></div><div class="s-item-subtitle" data-reactid="543"><div class="s-component s-text" data-reactid="544"><h6 class="s-component-content s-font-body" data-reactid="545"><p>Thanks to all the <a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Members">team members</a> including our instructors and advisers who make great contributes to the project. We are also flattered to introduce our sponsors and donators...</p></h6></div></div><div class="s-item-text" data-reactid="546"><div class="s-component s-text" data-reactid="547"><div class="s-component-content s-font-body" data-reactid="548"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Attributions"><strong>READ MORE</strong></a></p></div></div></div></div></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item s-block-smallFeatureRight " data-reactid="549"><span data-reactid="550"><div data-reactid="551"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="552"><div class="" data-reactid="553"><div class="s-block-feature " data-reactid="554"><div class="six columns right" data-reactid="555"><div class="s-item-media-group" data-reactid="556"><div class="s-component s-media " data-reactid="557"><span data-reactid="558"><div class="s-component-content" data-reactid="559"><div data-reactid="560"><a href="https://2016.igem.org/Team:BGIC_China/Collaborations" target="_blank" data-reactid="561"><div class="s-ratio-box" style="max-width:160px;max-height:160px;" data-reactid="562"><div class="s-ratio-fill" style="padding-bottom:100.00%;" data-reactid="563"></div><div data-reactid="564"><img alt="" title="" data-description="" width="160" height="160" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//uploads.strikinglycdn.com/static/icons/line-icons/34.png" data-sizes="auto" data-reactid="565"/><noscript data-reactid="566"><img alt="" title="" data-description="" width="160" height="160" src="//uploads.strikinglycdn.com/static/icons/line-icons/34.png" class="" data-reactid="567"/></noscript></div></div></a><!-- react-text: 568 --> <!-- /react-text --><!-- react-text: 569 --> <!-- /react-text --></div></div></span></div></div></div><div class="ten columns right" data-reactid="570"><div class="s-item-text-group half-offset-right" data-reactid="571"><div class="s-item-title" data-reactid="572"><div class="s-component s-text" data-reactid="573"><h3 class="s-component-content s-font-heading" data-reactid="574"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Collaborations"><strong>Collaborations</strong></a></p></h3></div></div><div class="s-item-subtitle" data-reactid="575"><div class="s-component s-text" data-reactid="576"><h6 class="s-component-content s-font-body" data-reactid="577"><p>We got a lot through our iGEM journey, and one of the best and most valuable among them was our cooperation with other iGEM team. In this win-win process, we exchanged our ideas, inspired creativity, and even helped each other to run experiments...</p></h6></div></div><div class="s-item-text" data-reactid="578"><div class="s-component s-text" data-reactid="579"><div class="s-component-content s-font-body" data-reactid="580"><p><a target="_blank" href="https://2016.igem.org/Team:BGIC_China/Collaborations"><strong>READ MORE</strong></a></p></div></div></div></div></div></div></div></div></div></span></div></div></div></div></div></li><li class="slide s-section-5 s-last-section-no-footer" id="section-f_0124394a-5c69-4b16-bade-3fb011802c26" data-reactid="581"><div class="waypoint" data-reactid="582"></div><a class="section-anchor" data-reactid="583"></a><div class="lazyload s-bg-image s-bg-light-text s-bg-overlay s-block-section s-section" style="background-repeat:repeat;background-size:auto;background-color:transparent;background-position:50% 50%;background-image:url(data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==);" data-bg="//uploads.strikinglycdn.com/static/backgrounds/pastel-solids/42.jpg" data-reactid="584"><div class="container" data-reactid="585"><div class="columns sixteen" data-reactid="586"><!-- react-empty: 587 --></div><div class="alignment-container " data-reactid="588"><div class="s-repeatable s-block s-component s-mh " data-reactid="589"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="590"><span data-reactid="591"><div data-reactid="592"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="593"><div class=" columns sixteen" data-reactid="594"><div class="s-component s-text" data-reactid="595"><div class="s-component-content s-font-body" data-reactid="596"><p>Sponsor</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="597"><span data-reactid="598"><div data-reactid="599"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="600"><div class=" columns sixteen" data-reactid="601"><div class="s-component s-image" data-reactid="602"><span data-reactid="603"><div class="s-component-content" data-reactid="604"><div data-reactid="605"><!-- react-text: 606 --> <!-- /react-text --><div class="s-ratio-box" style="max-width:141px;max-height:127px;" data-reactid="607"><div class="s-ratio-fill" style="padding-bottom:90.07%;" data-reactid="608"></div><div data-reactid="609"><img alt="" title="" data-description="" width="141" height="127" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/575969046670319095_%E5%89%AF%E6%9C%AC_u9h47r.png" data-sizes="auto" data-reactid="610"/><noscript data-reactid="611"><img alt="" title="" data-description="" width="141" height="127" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/575969046670319095_%E5%89%AF%E6%9C%AC_u9h47r.png" class="" data-reactid="612"/></noscript></div></div><!-- react-text: 613 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div></div></div></div></div><div class="waypoint" data-reactid="614"></div></li></ul><!-- react-empty: 615 --><div id="strikingly-tooltip-container" data-reactid="616"> </div><!-- react-empty: 617 --><!-- react-empty: 618 --><div class="s-floated-components" data-reactid="619"><div class="ecommerce-buy-dialog s-edit-modal extend" id="ecommerce-buy-dialog" data-reactid="620"><div class="s-async-wrapper s-component" data-reactid="621"><div class="s-loading" data-reactid="622"></div></div></div><div id="s-ecommerce-shopping-cart-wrapper" class="s-ecommerce-shopping-cart-wrapper" data-reactid="623"></div></div></div></div></div> <div id='fb-root'></div> <div id='app-script-root'></div> <div id='app-view-root'></div> <script id='blog-collection-tmpl' type='text/html'> <div class='s-blog-col-head'></div> <div class='s-blog-col-body'> {{ _.each(blogPosts, function(blog) { }} <div class='s-blog-entry'> <div class='s-blog-entry-inner'> {{ if ($S && $S.conf && $S.conf.preview_mode) { }} <a class='s-blog-overlay-link' href='{{=blog.publicUrl}}'>&nbsp;</a> {{ } else { }} <a class='s-blog-overlay-link' href='{{=blog.relativeUrl}}'>&nbsp;</a> {{ } }} {{ if (blog.iconUrl) { }} <div class='s-blog-entry-left'> {{ if (UrlHelper.imageHasContent(blog.iconUrl)) { }} <div class='s-blog-avatar' style='background-image:url({{=blog.iconUrl}});'></div> {{ } else { }} <div class='s-blog-default-avatar'> <div class='entypo-bookmark'></div> </div> {{ } }} </div> {{ } }} <div class='s-blog-entry-right'> <div class='s-blog-details'> <div class='s-blog-details-head s-blog-row'> <div class='s-blog-title s-font-heading'> {{=blog.title}} </div> <div class='s-blog-date'> {{=blog.publishedAt}} </div> </div> {{ if (blogSettings.previewLayout == 1) { }} <div class='s-blog-details-blurb s-blog-row'> {{=blog.blurb}} </div> {{ } }} </div> </div> </div> </div> {{ }) }} {{ if (blogPosts.length == 0) { }} <div class='s-blog-entry'> <div class='s-common-status no-posts-error'> 没有博文。 </div> </div> {{ } }} </div> {{ if (blogPosts.length > 0) { }} <div class='s-blog-col-foot'> <a class='s-blog-link s-blog-next-link' href='javascript:void(0);'> &laquo; 更新 </a> &nbsp; <ul class='s-blog-pagination' style='display:none;'> <li> <a class='s-blog-link s-blog-pagination-page' href='javascript:void(0);'></a> </li> </ul> &nbsp; <a class='s-blog-link s-blog-prev-link' href='javascript:void(0);'> 其它 &raquo; </a> </div> {{ } }} </script> <script src="//cdn.bootcss.com/jquery/1.10.0/jquery.min.js"></script> <script>
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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</div>
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<div class="column half_size" >
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<h5>Tips</h5>
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<p>This wiki will be your team��s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don��t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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</div>
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<div class="column half_size" >
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</div>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">
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UPLOAD FILES
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</div>
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</div>
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</html>
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Latest revision as of 18:10, 18 October 2016

Ellz's Site Copy

  • Paper, but more than a paper

    Programmable and open-source Next Generation Bio-Chip (NGBC) based on cell-free system

  • Programmable

    Give bio-chip different functions by re-construct immobilized genetic circuits.

    Cell-Free

    Bio-safety, low noise and more possibilities.

    Open-Source

    Distribute technology around the world.

    Short Prototyping Time

    Build a new bio-chip just with in a week.

    One-Step & High-Throughput

    Complete all the reactions on one bio-chip and test for multiple samples together.

    Ultra-High Expression

    U-HEC (Utra-High Expression Cassette) enables high protein expression on the bio-chip.

    Unlimited Potentials

    It is possible to testing for everything by construct proper genetic circuits.

  • Project Abstract

    NGBC --- Next Generation Bio-Chip, is what we are going to create. This will be the first bio-chip ever that allows user with knowledge in synthetic biology to easily re-program and re-construct it in order to make new bio-chips with different functions.

    NGBC use genetic circuits to process the input signals and given output results. In order to minimize the disruptions and limitations of biochemical reactions inside a living-cell, the reaction center of NGBC will use S30 cell-free system which is produced from cell extracts and could provide all the conditions required for biochemical reactions like that inside a real living-cell. What is most amazing is that the complete genetic circuits and cell-free system is just carried by simple filter paper and no other expensive materials required to construct it. We are also developing a hardware and a software which can be integrated with NGBC to provide a more accurate data readout and a more interactive user environment.

    In future, we intended to develop a completely open-source NGBC kit which contains all the necessary materials, protocols and genetic components required to re-program the BioChip, and the kit will be distributed to labs around the world so there will be more new BioChips with different functions be developed. We will also create an NGBC clinical database that includes all the verified BioChips which are able to measure the concentration of target clinical molecules such as glucose, nitrate and fatty-acids in the clinical sample with in a certain range.

  • We are going to design the first programmable BioChip ever by using genetic boolean logic gates(Piro et al, 2013) which enable the BioChip to achieve complex tasks. Several other amazing features like cell-free expression system and ultra-high expression cassette(U-HEC) are also introduced...

    (This part includes Mathematical Modelling and Demonstration)

    In order to prove the feasibility of our idea, we constructed a simple plasmid with a pH inducible promoter with EGFP coding sequence in the downstream of the promoter. We immobilized this plasmid onto our bio-chip system and tested for the RFU value in comparison with a standard constitutive J23119 promoter ...

    BBa_K2026000 --- a composite part with two B0015 sequence flanked by two same directional loxp sites.

    BBa_K2026001 --- pH and glucose inducible ultra-strong promoter from Cpx regulon.

    We introduced our project to visitors in London International Youth Science Forum (LIYSF) and spread the concept of synthetic biology including bio-safety to high school students and Bio-Maker organisations...

    Thanks to all the team members including our instructors and advisers who make great contributes to the project. We are also flattered to introduce our sponsors and donators...

    We got a lot through our iGEM journey, and one of the best and most valuable among them was our cooperation with other iGEM team. In this win-win process, we exchanged our ideas, inspired creativity, and even helped each other to run experiments...