Difference between revisions of "Team:BroadRun-Baltimore/Parts"

Line 15: Line 15:
 
th {
 
th {
 
     text-align: left;
 
     text-align: left;
 +
    font-size: 20px;
 
}
 
}
 +
 +
td
 +
{
 +
text-align: left;
 +
font-size: 17px;
  
 
</style>
 
</style>
Line 28: Line 34:
  
 
<h1>Parts</h1>
 
<h1>Parts</h1>
−
 
−
<style type="text/css">
 
−
<!--
 
−
.tab { margin-left: 40px; }
 
−
-->
 
−
 
−
table, th, td {
 
−
    border: 1px solid black;
 
−
    border-collapse: collapse;
 
−
}
 
−
 
−
 
−
th, td {
 
−
    padding: 15px;
 
−
}
 
−
 
−
th {
 
−
    text-align: left;
 
−
    font-size: 25px;
 
−
}
 
−
 
−
td
 
−
{
 
−
    text-align: left;
 
−
    font-size: 20px;
 
−
 
−
}
 
−
 
−
</style>
 
−
</head>
 
−
 
−
 
−
<div class="column full_size">
 
−
<body>
 
−
 
  
 
<hr>
 
<hr>

Revision as of 18:29, 18 October 2016

.MathJax nobr>span.math>span{border-left-width:0 !important};








Parts


New Parts Submitted to the Registry in 2016

Part Name Part Number
Penicillium alpha amylase w/ no promoter BBa_K2185003
B.cereus beta amylase w/ no promoter BBa_K2185005
S.ferax alpha amylase w/ cyc promoter BBa_K2185007

Composite Part 1: BBa_K2185003

Promoterless Kozak sequence (BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from the fungus Penicillium ADH1 Terminator (Part BBa_K392003)

Composite Part 2: BBa_K2185005

Promoterless Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Beta amylase coding sequence from Bacillus cereus ADH1 Terminator (Part BBa_K392003)

Composite Part 3: BBa_K2185007

pCyc medium promoter (BBa_I766555) Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from Saprolegnia ferax ADH1 Terminator (Part BBa_K392003)

Description of Basic Parts:

ADH1 Terminator - This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the DNA sequence.

Kozak Sequence - This part initiates translation from eukaryotic mRNA and is placed between a promoter and coding sequence to facilitate translation.

pCyc medium promoter - This is a medium expression level constitutive promoter. It directs the cell to transcribe the DNA sequence constantly.

Mating Factor Alpha Secretion Sequence - This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.

Penicillium Alpha Amylase - Obtained from the fungus Penicillium, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

S.ferax Alpha Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

B.cereus Beta Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses beta bonds of large, beta-linked polysaccharides, such as cellulose and some starches, thus degrading the polysaccharides into monosaccharides and disaccharides.

In addition to the 3 new parts submitted this year, we also improved a previous part, from last year. To see how we improved the characterization of this part, please see here.