Difference between revisions of "Team:BGIC China/Model"
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{{BGIC_China}} | {{BGIC_China}} | ||
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Thus viscosity can be seen as a constant.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eK is a constant and is provided by the producer of filter paper.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003e△p in a horizontal placed paper chip with height h which is much smaller than width(h\u0026lt;\u0026lt;w) can be calculated using formula (2):\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003e \u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_a14a6a08-8839-4e07-8fb8-e9ef7ddbcdf9","defaultValue":false,"link_url":"https://static.igem.org/mediawiki/2016/a/ad/T--BGIC_China--2.gif","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/2_jwfhg5","storage":"c","format":"gif","h":37,"w":181,"s":804,"new_target":true},{"type":"RichText","id":"f_d59da027-18e7-4661-bda6-3cca7057c72f","defaultValue":false,"value":"\u003cp style=\"text-align: center;\"\u003e\u003cem\u003e\u003cstrong\u003eFig.2 Formula (2)\u003c/strong\u003e\u003c/em\u003e\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"RichText","id":"f_9d0215ad-902c-45cf-bce8-ba87d2d2dabb","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\"\u003eγ is the surface tension of plasma. When blood keeps evaporating, its surface tension is less than 60mJ/m^2 in the liquid/vapor interface.60 can be taken as an extreme value.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eH is the height of solution, which can be seen as the same with the height of tunnels in the paper chip.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eCosθis the contact angle between air and the surface of solution.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eFrom formula (2) we can see that △p is constant with constant temperature, constant thickness of paper chip and within the same interface. Thus we see it as a constant value in the practical.\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_dcdfb18e-0493-49b6-b950-23fdc09268fb","defaultValue":false,"link_url":"https://static.igem.org/mediawiki/2016/f/fa/T--BGIC_China--8.png","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/8_kuyp4e","storage":"c","format":"png","h":208,"w":425,"s":1145,"new_target":true},{"type":"RichText","id":"f_bcca63f0-9f92-4a02-8455-9427b324e56c","defaultValue":false,"value":"\u003cp style=\"text-align: center;\"\u003e\u003cem\u003e\u003cstrong\u003eFig.3 A sketch of the reaction region of the test paper\u003c/strong\u003e\u003c/em\u003e\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"RichText","id":"f_f53a159f-5248-477c-b636-dae3bacd0415","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\"\u003eThis is a sketch of the reaction region of the test paper. Assumed that the length and width of each region are the same, and the length and width of each tubule also are the same, the whole test paper can be seen as a combination of four tubules and four square reaction regions.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eLet the length, width and thickness of reaction region be L1, W1 and H1 and those of tubules be L2, W2 and H2. Thus the formula can be simplified to:\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003e \u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_86522c6e-cc76-4409-84ba-b2b8f324c909","defaultValue":false,"link_url":"https://static.igem.org/mediawiki/2016/c/c4/T--BGIC_China--4.gif","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/4_ikycsm","storage":"c","format":"gif","h":41,"w":247,"s":1257,"new_target":true},{"type":"RichText","id":"f_44d09d34-9173-46b1-8402-b9d05ff98b08","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\"\u003eIn this formula,\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_1ea7aa01-f6e4-4a00-a324-5a868a751d58","defaultValue":false,"link_url":"https://static.igem.org/mediawiki/2016/9/9f/T--BGIC_China--5.gif","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/5_nlphxx","storage":"c","format":"gif","h":18,"w":227,"s":887,"new_target":true},{"type":"RichText","id":"f_bb04fafa-e844-4449-be8f-ed9dc6f6c8f1","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\"\u003eAnd the thickness is the same in every part of the test paper, so H1 = H2. Then we can cancel out the thickness in the formula to get:\u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_fb0883c9-a337-4539-a0bf-9164177b3e8c","defaultValue":false,"link_url":"https://static.igem.org/mediawiki/2016/e/e6/T--BGIC_China--6.gif","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/6_ogf0sd","storage":"c","format":"gif","h":45,"w":311,"s":1660,"new_target":true},{"type":"RichText","id":"f_6b2b7898-0ca8-4990-b8ae-0dfe2f2b9fe4","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\"\u003eμ, k and △p are all constants.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eSo the time taken t is directly proportional to the product of (L1W1+L2W2)(L1/W1+L2/W2).\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eAnd it is also proved that the time taken is reversely proportional to L for reaction regions and W2/W1, but directly proportional to the area of tubules. In consideration of the reader and size of the filter paper (whatman 5), we set up a few experimental groups of test paper with different width and length.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eTable:\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003e \u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1},{"type":"Image","id":"f_d3582aff-c9f9-46b1-936e-820d6c39ae54","defaultValue":false,"link_url":"https://static.igem.org/mediawiki/2016/0/02/T--BGIC_China--10.png","thumb_url":"!","url":"!","caption":"","description":"","storageKey":"791914/10_v0xjiw","storage":"c","format":"png","h":364,"w":707,"s":10118,"new_target":true},{"type":"RichText","id":"f_c72962ef-e544-4169-9818-7078b7b4b74f","defaultValue":false,"value":"\u003cp style=\"font-size: 130%;\"\u003eThe time taken for water to travel to the end of the test paper is recorded in the experiment and a graph is plotted for time against the product of (L1W1+L2W2)(L1/W1+L2/W2).\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eThen we do a linear regression to find the best-fit line of the graph. The gradient of this line equals to 16μ/k△p. And the size of test paper which takes the least time is found.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003e \u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003eMoreover, in order to read the result more easily, we also have another design of test paper, which coincide with the size of 96-well plates. This kind of test paper can put on the elisa reader and test for the result directly.\u003c/p\u003e\u003cp style=\"font-size: 130%;\"\u003e \u003c/p\u003e","backupValue":"在此处添加文本段落。","version":1}]}}}],"title":null,"uid":null,"path":null,"autoPath":null}],"menu":{"type":"Menu","id":"f_54310d53-5ba2-4793-bd59-b00da4081629","defaultValue":null,"template_name":"navbar","logo":null,"components":{"background1":{"type":"Background","id":"f_76c941dc-c059-4061-ba2c-b0642a1f396b","defaultValue":true,"url":"/assets/themes/profile/bg.jpg","textColor":"light","backgroundVariation":"","sizing":"cover","videoUrl":"","videoHtml":"","storageKey":null,"storage":null,"format":null,"h":null,"w":null,"s":null},"image1":{"type":"Image","id":"f_a8010fa7-c561-4eea-8293-793825093faf","defaultValue":true,"link_url":null,"thumb_url":null,"url":"http://assets.strikingly.com/assets/themes/sleek/logo.png","caption":"","description":"","storageKey":null,"storage":null,"format":null,"h":null,"w":null,"s":null,"new_target":true},"image2":{"type":"Image","id":"f_8e37c922-ca0c-408d-9623-00fd9782d212","defaultValue":true,"link_url":null,"thumb_url":null,"url":"http://assets.strikingly.com/assets/themes/fresh/power.png","caption":"","description":"","storageKey":null,"storage":null,"format":null,"h":null,"w":null,"s":null,"new_target":true},"text1":{"type":"RichText","id":"f_b91c547a-71fd-412c-bbe0-ae1233468cf3","defaultValue":true,"value":"Title Text","backupValue":null,"version":null},"text2":{"type":"RichText","id":"f_ddec1bbb-3d45-468d-9a29-ec30cb3ae201","defaultValue":true,"value":"Subtitle Text","backupValue":null,"version":null},"button1":{"type":"Button","id":"f_81f80351-4ea1-4e4e-b0e9-c09e7342fd3d","defaultValue":true,"text":"Add a button","url":"http://strikingly.com","new_target":null}}},"footer":{"type":"Footer","id":"f_2d75fc34-40d5-416b-bb48-e437be8b670c","defaultValue":null,"socialMedia":null,"copyright":null,"components":{"socialMedia":{"type":"SocialMediaList","id":"f_52dc7699-9380-4e8d-9500-e51618c95e8f","defaultValue":false,"link_list":[{"type":"Facebook","id":"f_90b4be82-1a25-42d9-8160-4aa2fa7c48a9","defaultValue":null,"url":"","link_url":null,"share_text":null,"app_id":null,"show_button":true},{"type":"Twitter","id":"f_85287602-d59a-43fd-8845-fac8161206bc","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":true},{"type":"GPlus","id":"f_d4e77048-3568-4f35-b0cd-cbe57eeee145","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false},{"type":"LinkedIn","id":"f_b01e265e-b136-4725-9cde-97ef60d0261e","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false},{"type":"Instagram","id":"f_118b9462-0d12-4ec5-9d5a-95f2af2f53fa","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false}],"button_list":[{"type":"Facebook","id":"f_55cb59a1-a151-4ade-bd94-cd8022bf954b","defaultValue":null,"url":"","link_url":"","share_text":"","app_id":543870062356274,"show_button":true},{"type":"Twitter","id":"f_48056a2f-5715-4d2a-8523-3a6ad0f2cbd0","defaultValue":null,"url":"","link_url":"","share_text":"","show_button":true},{"type":"GPlus","id":"f_31a4f1f7-2a6d-4159-90c7-121a859ce637","defaultValue":null,"url":"","link_url":"","share_text":"","show_button":true},{"type":"LinkedIn","id":"f_37c15ea1-fafe-4ce5-b15d-01a359744f1c","defaultValue":null,"url":"","link_url":"","share_text":"","show_button":false},{"type":"Pinterest","id":"f_0e26ea8c-251c-414f-bf45-f3dd324cba3e","defaultValue":null,"url":"","link_url":null,"share_text":null,"show_button":false}],"list_type":null},"copyright":{"type":"RichText","id":"f_287cb485-8a5e-43d3-9cfb-25574bc440fe","defaultValue":false,"value":"\u003cp\u003eCopyright © 2002 - 2016 Eckhart Consulting. 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| − | + | </script> <!--[if lte IE 9]><script src="//assets.strikingly.com/assets/detectIE-b0fc7986f8eb7d40a31e9582bdeb7b88.js"></script><![endif]--> </head> <body class='show locale-zh-CN' id='user_sites'> <div id="s-page-container"><div data-reactroot="" data-reactid="1" data-react-checksum="1423281276"><!-- react-empty: 2 --><div id="s-content" class="s-variation-default s-font-heading-default s-font-title-default s-font-body-default" data-reactid="3"><div class="social-thumbnail" data-reactid="4"><div style="display:none;" data-reactid="5"><img src="//assets.strikingly.com/assets/fb_images/default-715654470f8f9a657474bf68cefc382f.png" alt="page thumbnail" width="300" height="300" data-reactid="6"/></div><div id="lw-share-data" style="display:none;" data-reactid="7"><div class="lws-title" data-reactid="8">Ellz's Site Copy Copy</div><div class="lws-description" data-reactid="9">Website</div><div class="lws-image" data-reactid="10">http://assets.strikingly.com/assets/fb_images/default-715654470f8f9a657474bf68cefc382f.png</div></div></div><!-- react-empty: 11 --><!-- react-empty: 12 --><ul class="slides s-page-1" data-reactid="13"><li class="slide s-section-1" id="section-f_f8c5c98a-1cab-4028-b3ec-94c4d390ad57" data-reactid="14"><div class="waypoint" data-reactid="15"></div><a class="section-anchor" data-reactid="16"></a><div class="lazyload s-block-section s-section" data-reactid="17"><div class="container" data-reactid="18"><div class="columns sixteen" data-reactid="19"><!-- react-empty: 20 --></div><div class="alignment-container " data-reactid="21"><div class="s-repeatable s-block s-component s-mh " data-reactid="22"><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="23"><span data-reactid="24"><div data-reactid="25"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="26"><div class=" columns sixteen" data-reactid="27"><div class="s-component s-image" data-reactid="28"><span data-reactid="29"><div class="s-component-content" data-reactid="30"><div data-reactid="31"><a href="https://static.igem.org/mediawiki/2016/e/ec/T--BGIC_China--1.gif" target="_blank" data-reactid="32"><div class="s-ratio-box" style="max-width:145px;max-height:51px;" data-reactid="33"><div class="s-ratio-fill" style="padding-bottom:35.17%;" data-reactid="34"></div><div data-reactid="35"><img alt="" title="" data-description="" width="145" height="51" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/1_oe2j1y.gif" data-sizes="auto" data-reactid="36"/><noscript data-reactid="37"><img alt="" title="" data-description="" width="145" height="51" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/1_oe2j1y.gif" class="" data-reactid="38"/></noscript></div></div></a><!-- react-text: 39 --> <!-- /react-text --><!-- react-text: 40 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="41"><span data-reactid="42"><div data-reactid="43"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="44"><div class=" columns sixteen" data-reactid="45"><div class="s-component s-text" data-reactid="46"><div class="s-component-content s-font-body" data-reactid="47"><p style="text-align: center;"><em><strong>Fig.1 Formula (1) The relationship between time and width and length of each part of paper chip. </strong></em></p><p style="text-align: center;"><em>T is the time taken for solution to diffuse from one end to another of the paper chip.</em></p><p style="text-align: center;"><em>V is the volume of paper chip.</em></p><p style="text-align: center;"><em>μ is the viscosity of solution.</em></p><p style="text-align: center;"><em>K is the permeability.</em></p><p style="text-align: center;"><em>△p is the difference in pressure.</em></p><p style="text-align: center;"><em>L is the length of a part of paper chip.</em></p><p style="text-align: center;"><em>W is the width of a part of paper chip.</em></p><p style="text-align: center;"><em>H is the thickness of a part of paper chip.</em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="48"><span data-reactid="49"><div data-reactid="50"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="51"><div class=" columns sixteen" data-reactid="52"><div class="s-component s-text" data-reactid="53"><div class="s-component-content s-font-body" data-reactid="54"><p style="font-size: 130%;">In practical, as the viscosity of plasma, which is 1.2-1.66, is almost equal to viscosity of water, water can be used instead of plasma. And the effect of concentration of glucose to the plasma viscosity can also be ignored. Thus viscosity can be seen as a constant.</p><p style="font-size: 130%;">K is a constant and is provided by the producer of filter paper.</p><p style="font-size: 130%;">△p in a horizontal placed paper chip with height h which is much smaller than width(h<<w) can be calculated using formula (2):</p><p style="font-size: 130%;"> </p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="55"><span data-reactid="56"><div data-reactid="57"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="58"><div class=" columns sixteen" data-reactid="59"><div class="s-component s-image" data-reactid="60"><span data-reactid="61"><div class="s-component-content" data-reactid="62"><div data-reactid="63"><a href="https://static.igem.org/mediawiki/2016/a/ad/T--BGIC_China--2.gif" target="_blank" data-reactid="64"><div class="s-ratio-box" style="max-width:181px;max-height:37px;" data-reactid="65"><div class="s-ratio-fill" style="padding-bottom:20.44%;" data-reactid="66"></div><div data-reactid="67"><img alt="" title="" data-description="" width="181" height="37" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/2_jwfhg5.gif" data-sizes="auto" data-reactid="68"/><noscript data-reactid="69"><img alt="" title="" data-description="" width="181" height="37" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/2_jwfhg5.gif" class="" data-reactid="70"/></noscript></div></div></a><!-- react-text: 71 --> <!-- /react-text --><!-- react-text: 72 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="73"><span data-reactid="74"><div data-reactid="75"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="76"><div class=" columns sixteen" data-reactid="77"><div class="s-component s-text" data-reactid="78"><div class="s-component-content s-font-body" data-reactid="79"><p style="text-align: center;"><em><strong>Fig.2 Formula (2)</strong></em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="80"><span data-reactid="81"><div data-reactid="82"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="83"><div class=" columns sixteen" data-reactid="84"><div class="s-component s-text" data-reactid="85"><div class="s-component-content s-font-body" data-reactid="86"><p style="font-size: 130%;">γ is the surface tension of plasma. When blood keeps evaporating, its surface tension is less than 60mJ/m^2 in the liquid/vapor interface.60 can be taken as an extreme value.</p><p style="font-size: 130%;">H is the height of solution, which can be seen as the same with the height of tunnels in the paper chip.</p><p style="font-size: 130%;">Cosθis the contact angle between air and the surface of solution.</p><p style="font-size: 130%;">From formula (2) we can see that △p is constant with constant temperature, constant thickness of paper chip and within the same interface. Thus we see it as a constant value in the practical.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="87"><span data-reactid="88"><div data-reactid="89"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="90"><div class=" columns sixteen" data-reactid="91"><div class="s-component s-image" data-reactid="92"><span data-reactid="93"><div class="s-component-content" data-reactid="94"><div data-reactid="95"><a href="https://static.igem.org/mediawiki/2016/f/fa/T--BGIC_China--8.png" target="_blank" data-reactid="96"><div class="s-ratio-box" style="max-width:425px;max-height:208px;" data-reactid="97"><div class="s-ratio-fill" style="padding-bottom:48.94%;" data-reactid="98"></div><div data-reactid="99"><img alt="" title="" data-description="" width="425" height="208" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/8_kuyp4e.png" data-sizes="auto" data-reactid="100"/><noscript data-reactid="101"><img alt="" title="" data-description="" width="425" height="208" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/8_kuyp4e.png" class="" data-reactid="102"/></noscript></div></div></a><!-- react-text: 103 --> <!-- /react-text --><!-- react-text: 104 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="105"><span data-reactid="106"><div data-reactid="107"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="108"><div class=" columns sixteen" data-reactid="109"><div class="s-component s-text" data-reactid="110"><div class="s-component-content s-font-body" data-reactid="111"><p style="text-align: center;"><em><strong>Fig.3 A sketch of the reaction region of the test paper</strong></em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="112"><span data-reactid="113"><div data-reactid="114"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="115"><div class=" columns sixteen" data-reactid="116"><div class="s-component s-text" data-reactid="117"><div class="s-component-content s-font-body" data-reactid="118"><p style="font-size: 130%;">This is a sketch of the reaction region of the test paper. Assumed that the length and width of each region are the same, and the length and width of each tubule also are the same, the whole test paper can be seen as a combination of four tubules and four square reaction regions.</p><p style="font-size: 130%;">Let the length, width and thickness of reaction region be L1, W1 and H1 and those of tubules be L2, W2 and H2. Thus the formula can be simplified to:</p><p style="font-size: 130%;"> </p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="119"><span data-reactid="120"><div data-reactid="121"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="122"><div class=" columns sixteen" data-reactid="123"><div class="s-component s-image" data-reactid="124"><span data-reactid="125"><div class="s-component-content" data-reactid="126"><div data-reactid="127"><a href="https://static.igem.org/mediawiki/2016/c/c4/T--BGIC_China--4.gif" target="_blank" data-reactid="128"><div class="s-ratio-box" style="max-width:247px;max-height:41px;" data-reactid="129"><div class="s-ratio-fill" style="padding-bottom:16.60%;" data-reactid="130"></div><div data-reactid="131"><img alt="" title="" data-description="" width="247" height="41" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/4_ikycsm.gif" data-sizes="auto" data-reactid="132"/><noscript data-reactid="133"><img alt="" title="" data-description="" width="247" height="41" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/4_ikycsm.gif" class="" data-reactid="134"/></noscript></div></div></a><!-- react-text: 135 --> <!-- /react-text --><!-- react-text: 136 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="137"><span data-reactid="138"><div data-reactid="139"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="140"><div class=" columns sixteen" data-reactid="141"><div class="s-component s-text" data-reactid="142"><div class="s-component-content s-font-body" data-reactid="143"><p style="font-size: 130%;">In this formula,</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="144"><span data-reactid="145"><div data-reactid="146"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="147"><div class=" columns sixteen" data-reactid="148"><div class="s-component s-image" data-reactid="149"><span data-reactid="150"><div class="s-component-content" data-reactid="151"><div data-reactid="152"><a href="https://static.igem.org/mediawiki/2016/9/9f/T--BGIC_China--5.gif" target="_blank" data-reactid="153"><div class="s-ratio-box" style="max-width:227px;max-height:18px;" data-reactid="154"><div class="s-ratio-fill" style="padding-bottom:7.93%;" data-reactid="155"></div><div data-reactid="156"><img alt="" title="" data-description="" width="227" height="18" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/5_nlphxx.gif" data-sizes="auto" data-reactid="157"/><noscript data-reactid="158"><img alt="" title="" data-description="" width="227" height="18" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/5_nlphxx.gif" class="" data-reactid="159"/></noscript></div></div></a><!-- react-text: 160 --> <!-- /react-text --><!-- react-text: 161 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="162"><span data-reactid="163"><div data-reactid="164"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="165"><div class=" columns sixteen" data-reactid="166"><div class="s-component s-text" data-reactid="167"><div class="s-component-content s-font-body" data-reactid="168"><p style="font-size: 130%;">And the thickness is the same in every part of the test paper, so H1 = H2. Then we can cancel out the thickness in the formula to get:</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="169"><span data-reactid="170"><div data-reactid="171"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="172"><div class=" columns sixteen" data-reactid="173"><div class="s-component s-image" data-reactid="174"><span data-reactid="175"><div class="s-component-content" data-reactid="176"><div data-reactid="177"><a href="https://static.igem.org/mediawiki/2016/e/e6/T--BGIC_China--6.gif" target="_blank" data-reactid="178"><div class="s-ratio-box" style="max-width:311px;max-height:45px;" data-reactid="179"><div class="s-ratio-fill" style="padding-bottom:14.47%;" data-reactid="180"></div><div data-reactid="181"><img alt="" title="" data-description="" width="311" height="45" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/6_ogf0sd.gif" data-sizes="auto" data-reactid="182"/><noscript data-reactid="183"><img alt="" title="" data-description="" width="311" height="45" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/6_ogf0sd.gif" class="" data-reactid="184"/></noscript></div></div></a><!-- react-text: 185 --> <!-- /react-text --><!-- react-text: 186 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="187"><span data-reactid="188"><div data-reactid="189"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="190"><div class=" columns sixteen" data-reactid="191"><div class="s-component s-text" data-reactid="192"><div class="s-component-content s-font-body" data-reactid="193"><p style="font-size: 130%;">μ, k and △p are all constants.</p><p style="font-size: 130%;">So the time taken t is directly proportional to the product of (L1W1+L2W2)(L1/W1+L2/W2).</p><p style="font-size: 130%;">And it is also proved that the time taken is reversely proportional to L for reaction regions and W2/W1, but directly proportional to the area of tubules. In consideration of the reader and size of the filter paper (whatman 5), we set up a few experimental groups of test paper with different width and length.</p><p style="font-size: 130%;">Table:</p><p style="font-size: 130%;"> </p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="194"><span data-reactid="195"><div data-reactid="196"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="197"><div class=" columns sixteen" data-reactid="198"><div class="s-component s-image" data-reactid="199"><span data-reactid="200"><div class="s-component-content" data-reactid="201"><div data-reactid="202"><a href="https://static.igem.org/mediawiki/2016/0/02/T--BGIC_China--10.png" target="_blank" data-reactid="203"><div class="s-ratio-box" style="max-width:707px;max-height:364px;" data-reactid="204"><div class="s-ratio-fill" style="padding-bottom:51.49%;" data-reactid="205"></div><div data-reactid="206"><img alt="" title="" data-description="" width="707" height="364" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/10_v0xjiw.png" data-sizes="auto" data-reactid="207"/><noscript data-reactid="208"><img alt="" title="" data-description="" width="707" height="364" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/10_v0xjiw.png" class="" data-reactid="209"/></noscript></div></div></a><!-- react-text: 210 --> <!-- /react-text --><!-- react-text: 211 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="212"><span data-reactid="213"><div data-reactid="214"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="215"><div class=" columns sixteen" data-reactid="216"><div class="s-component s-text" data-reactid="217"><div class="s-component-content s-font-body" data-reactid="218"><p style="font-size: 130%;">The time taken for water to travel to the end of the test paper is recorded in the experiment and a graph is plotted for time against the product of (L1W1+L2W2)(L1/W1+L2/W2).</p><p style="font-size: 130%;">Then we do a linear regression to find the best-fit line of the graph. The gradient of this line equals to 16μ/k△p. And the size of test paper which takes the least time is found.</p><p style="font-size: 130%;"> </p><p style="font-size: 130%;">Moreover, in order to read the result more easily, we also have another design of test paper, which coincide with the size of 96-well plates. This kind of test paper can put on the elisa reader and test for the result directly.</p><p style="font-size: 130%;"> </p></div></div></div></div></div></span></div></div></div></div></div></li></ul><!-- react-empty: 219 --><div id="strikingly-tooltip-container" data-reactid="220"> </div><!-- react-empty: 221 --><!-- react-empty: 222 --><div class="s-floated-components" data-reactid="223"><div class="ecommerce-buy-dialog s-edit-modal extend" id="ecommerce-buy-dialog" data-reactid="224"><div class="s-async-wrapper s-component" data-reactid="225"><div class="s-loading" data-reactid="226"></div></div></div><div id="s-ecommerce-shopping-cart-wrapper" class="s-ecommerce-shopping-cart-wrapper" data-reactid="227"></div></div></div></div></div> <div id='fb-root'></div> <div id='app-script-root'></div> <div id='app-view-root'></div> <script id='blog-collection-tmpl' type='text/html'> <div class='s-blog-col-head'></div> <div class='s-blog-col-body'> {{ _.each(blogPosts, function(blog) { }} <div class='s-blog-entry'> <div class='s-blog-entry-inner'> {{ if ($S && $S.conf && $S.conf.preview_mode) { }} <a class='s-blog-overlay-link' href='{{=blog.publicUrl}}'> </a> {{ } else { }} <a class='s-blog-overlay-link' href='{{=blog.relativeUrl}}'> </a> {{ } }} {{ if (blog.iconUrl) { }} <div class='s-blog-entry-left'> {{ if (UrlHelper.imageHasContent(blog.iconUrl)) { }} <div class='s-blog-avatar' style='background-image:url({{=blog.iconUrl}});'></div> {{ } else { }} <div class='s-blog-default-avatar'> <div class='entypo-bookmark'></div> </div> {{ } }} </div> {{ } }} <div class='s-blog-entry-right'> <div class='s-blog-details'> <div class='s-blog-details-head s-blog-row'> <div class='s-blog-title s-font-heading'> {{=blog.title}} </div> <div class='s-blog-date'> {{=blog.publishedAt}} </div> </div> {{ if (blogSettings.previewLayout == 1) { }} <div class='s-blog-details-blurb s-blog-row'> {{=blog.blurb}} </div> {{ } }} </div> </div> </div> </div> {{ }) }} {{ if (blogPosts.length == 0) { }} <div class='s-blog-entry'> <div class='s-common-status no-posts-error'> 没有博文。 </div> </div> {{ } }} </div> {{ if (blogPosts.length > 0) { }} <div class='s-blog-col-foot'> <a class='s-blog-link s-blog-next-link' href='javascript:void(0);'> « 更新 </a> <ul class='s-blog-pagination' style='display:none;'> <li> <a class='s-blog-link s-blog-pagination-page' href='javascript:void(0);'></a> </li> </ul> <a class='s-blog-link s-blog-prev-link' href='javascript:void(0);'> 其它 » </a> </div> {{ } }} </script> <script src="//cdn.bootcss.com/jquery/1.10.0/jquery.min.js"></script> <script> | |
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Latest revision as of 19:23, 18 October 2016
Fig.1 Formula (1) The relationship between time and width and length of each part of paper chip.
T is the time taken for solution to diffuse from one end to another of the paper chip.
V is the volume of paper chip.
μ is the viscosity of solution.
K is the permeability.
△p is the difference in pressure.
L is the length of a part of paper chip.
W is the width of a part of paper chip.
H is the thickness of a part of paper chip.
In practical, as the viscosity of plasma, which is 1.2-1.66, is almost equal to viscosity of water, water can be used instead of plasma. And the effect of concentration of glucose to the plasma viscosity can also be ignored. Thus viscosity can be seen as a constant.
K is a constant and is provided by the producer of filter paper.
△p in a horizontal placed paper chip with height h which is much smaller than width(h<<w) can be calculated using formula (2):
Fig.2 Formula (2)
γ is the surface tension of plasma. When blood keeps evaporating, its surface tension is less than 60mJ/m^2 in the liquid/vapor interface.60 can be taken as an extreme value.
H is the height of solution, which can be seen as the same with the height of tunnels in the paper chip.
Cosθis the contact angle between air and the surface of solution.
From formula (2) we can see that △p is constant with constant temperature, constant thickness of paper chip and within the same interface. Thus we see it as a constant value in the practical.
Fig.3 A sketch of the reaction region of the test paper
This is a sketch of the reaction region of the test paper. Assumed that the length and width of each region are the same, and the length and width of each tubule also are the same, the whole test paper can be seen as a combination of four tubules and four square reaction regions.
Let the length, width and thickness of reaction region be L1, W1 and H1 and those of tubules be L2, W2 and H2. Thus the formula can be simplified to:
In this formula,
And the thickness is the same in every part of the test paper, so H1 = H2. Then we can cancel out the thickness in the formula to get:
μ, k and △p are all constants.
So the time taken t is directly proportional to the product of (L1W1+L2W2)(L1/W1+L2/W2).
And it is also proved that the time taken is reversely proportional to L for reaction regions and W2/W1, but directly proportional to the area of tubules. In consideration of the reader and size of the filter paper (whatman 5), we set up a few experimental groups of test paper with different width and length.
Table:
The time taken for water to travel to the end of the test paper is recorded in the experiment and a graph is plotted for time against the product of (L1W1+L2W2)(L1/W1+L2/W2).
Then we do a linear regression to find the best-fit line of the graph. The gradient of this line equals to 16μ/k△p. And the size of test paper which takes the least time is found.
Moreover, in order to read the result more easily, we also have another design of test paper, which coincide with the size of 96-well plates. This kind of test paper can put on the elisa reader and test for the result directly.
