Difference between revisions of "Team:BGIC China/Demonstrate"
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{{BGIC_China}} | {{BGIC_China}} | ||
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We made the carrier of genetic circuits by filter paper manufactured by Whatman®. The black area of the filter paper is wax printed by wax-printer.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="45"><span data-reactid="46"><div data-reactid="47"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="48"><div class=" columns sixteen" data-reactid="49"><div class="s-component s-image" data-reactid="50"><span data-reactid="51"><div class="s-component-content" data-reactid="52"><div data-reactid="53"><a href="https://static.igem.org/mediawiki/2016/0/01/T--BGIC_China--545947305911474069.jpg" target="_blank" data-reactid="54"><div class="s-ratio-box" style="max-width:2048px;max-height:1536px;" data-reactid="55"><div class="s-ratio-fill" style="padding-bottom:75.00%;" data-reactid="56"></div><div data-reactid="57"><img alt="" title="" data-description="" width="2048" height="1536" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/545947305911474069_t58r1d.jpg" data-sizes="auto" data-reactid="58"/><noscript data-reactid="59"><img alt="" title="" data-description="" width="2048" height="1536" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/545947305911474069_t58r1d.jpg" class="" data-reactid="60"/></noscript></div></div></a><!-- react-text: 61 --> <!-- /react-text --><!-- react-text: 62 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="63"><span data-reactid="64"><div data-reactid="65"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="66"><div class=" columns sixteen" data-reactid="67"><div class="s-component s-text" data-reactid="68"><div class="s-component-content s-font-body" data-reactid="69"><p><em><strong>Fig.1 NGBC v0.1</strong></em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="70"><span data-reactid="71"><div data-reactid="72"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="73"><div class=" columns sixteen" data-reactid="74"><div class="s-component s-text" data-reactid="75"><div class="s-component-content s-font-body" data-reactid="76"><p style="font-size: 130%; text-align: center;"><em>Test for paper-based cell-free system</em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="77"><span data-reactid="78"><div data-reactid="79"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="80"><div class=" columns sixteen" data-reactid="81"><div class="s-component s-text" data-reactid="82"><div class="s-component-content s-font-body" data-reactid="83"><p style="font-size:130%">We first cutted the filter papers into small pieces of squares(3mm x 3mm) and placed them into wells of 384 well plates. 60ng of plasmid pCpxP-LacZ in 2ul of S30 cell-free system(pH = 7) and 400ng of X-gal (dissolved in DMSO; conc = 20ug/ul) was then added into each well and was immobilized onto filter papers by using freeze-dried technology. 2ul of PBS buffer solution(pH = 7) has then been added into each well to rehydrate the filter paper. After having reaction under 37℃ for 1 hour, we examined the result by looking at the colour of the filter paper. The change in colour of the filter paper demonstrated that the transcription and translation of β-galactosidase from LacZ gene had taken place (Fig.2 & Fig.3). Thus, it shows that the paper-based cell-free test paper works in laboratory condition.</p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="84"><span data-reactid="85"><div data-reactid="86"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="87"><div class=" columns sixteen" data-reactid="88"><div class="s-component s-image" data-reactid="89"><span data-reactid="90"><div class="s-component-content" data-reactid="91"><div data-reactid="92"><a href="https://static.igem.org/mediawiki/2016/b/bc/T--BGIC_China--%E6%9C%AA%E6%A0%87%E9%A2%98-3.png" target="_blank" data-reactid="93"><div class="s-ratio-box" style="max-width:403px;max-height:125px;" data-reactid="94"><div class="s-ratio-fill" style="padding-bottom:31.02%;" data-reactid="95"></div><div data-reactid="96"><img alt="" title="" data-description="" width="403" height="125" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/%E6%9C%AA%E6%A0%87%E9%A2%98-3_igxr8c.png" data-sizes="auto" data-reactid="97"/><noscript data-reactid="98"><img alt="" title="" data-description="" width="403" height="125" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/%E6%9C%AA%E6%A0%87%E9%A2%98-3_igxr8c.png" class="" data-reactid="99"/></noscript></div></div></a><!-- react-text: 100 --> <!-- /react-text --><!-- react-text: 101 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="102"><span data-reactid="103"><div data-reactid="104"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="105"><div class=" columns sixteen" data-reactid="106"><div class="s-component s-text" data-reactid="107"><div class="s-component-content s-font-body" data-reactid="108"><p><em><strong>Fig.2 Photo taken from 384 well plate.</strong> Left: Blank; Middle: Control; Right: pCpxP-LacZ immobilized filter paper.</em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="109"><span data-reactid="110"><div data-reactid="111"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="112"><div class=" columns sixteen" data-reactid="113"><div class="s-component s-image" data-reactid="114"><span data-reactid="115"><div class="s-component-content" data-reactid="116"><div data-reactid="117"><a href="https://static.igem.org/mediawiki/2016/c/c5/T--BGIC_China--%E6%9C%AA%E6%A0%87%E9%A2%98-1.png" target="_blank" data-reactid="118"><div class="s-ratio-box" style="max-width:397px;max-height:157px;" data-reactid="119"><div class="s-ratio-fill" style="padding-bottom:39.55%;" data-reactid="120"></div><div data-reactid="121"><img alt="" title="" data-description="" width="397" height="157" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/%E6%9C%AA%E6%A0%87%E9%A2%98-1_i5dptn.png" data-sizes="auto" data-reactid="122"/><noscript data-reactid="123"><img alt="" title="" data-description="" width="397" height="157" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto/v1/791914/%E6%9C%AA%E6%A0%87%E9%A2%98-1_i5dptn.png" class="" data-reactid="124"/></noscript></div></div></a><!-- react-text: 125 --> <!-- /react-text --><!-- react-text: 126 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="127"><span data-reactid="128"><div data-reactid="129"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="130"><div class=" columns sixteen" data-reactid="131"><div class="s-component s-text" data-reactid="132"><div class="s-component-content s-font-body" data-reactid="133"><p><em><strong>Fig.3 Picture generated by using average color depth of each cell. </strong>Left: Blank; Middle: Control; Right: pCpxP-LacZ immobilized filter paper.</em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="134"><span data-reactid="135"><div data-reactid="136"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="137"><div class=" columns sixteen" data-reactid="138"><div class="s-component s-text" data-reactid="139"><div class="s-component-content s-font-body" data-reactid="140"><p style="font-size: 130%; text-align: center;"><em>Supplementary pictures</em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="141"><span data-reactid="142"><div data-reactid="143"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="144"><div class=" columns sixteen" data-reactid="145"><div class="s-component s-image" data-reactid="146"><span data-reactid="147"><div class="s-component-content" data-reactid="148"><div data-reactid="149"><a href="https://static.igem.org/mediawiki/2016/0/03/T--BGIC_China--130362751177144957.jpg" target="_blank" data-reactid="150"><div class="s-ratio-box" style="max-width:2048px;max-height:1536px;" data-reactid="151"><div class="s-ratio-fill" style="padding-bottom:75.00%;" data-reactid="152"></div><div data-reactid="153"><img alt="" title="" data-description="" width="2048" height="1536" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/130362751177144957_q0ygmj.jpg" data-sizes="auto" data-reactid="154"/><noscript data-reactid="155"><img alt="" title="" data-description="" width="2048" height="1536" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/130362751177144957_q0ygmj.jpg" class="" data-reactid="156"/></noscript></div></div></a><!-- react-text: 157 --> <!-- /react-text --><!-- react-text: 158 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="159"><span data-reactid="160"><div data-reactid="161"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="162"><div class=" columns sixteen" data-reactid="163"><div class="s-component s-text" data-reactid="164"><div class="s-component-content s-font-body" data-reactid="165"><p><em><strong>Fig.4 </strong></em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="166"><span data-reactid="167"><div data-reactid="168"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="169"><div class=" columns sixteen" data-reactid="170"><div class="s-component s-image" data-reactid="171"><span data-reactid="172"><div class="s-component-content" data-reactid="173"><div data-reactid="174"><a href="https://static.igem.org/mediawiki/2016/9/9e/T--BGIC_China--683102739564180124_%E5%89%AF%E6%9C%AC.png" target="_blank" data-reactid="175"><div class="s-ratio-box" style="max-width:2048px;max-height:883px;" data-reactid="176"><div class="s-ratio-fill" style="padding-bottom:43.12%;" data-reactid="177"></div><div data-reactid="178"><img alt="" title="" data-description="" width="2048" height="883" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/683102739564180124_%E5%89%AF%E6%9C%AC_f1ny8y.png" data-sizes="auto" data-reactid="179"/><noscript data-reactid="180"><img alt="" title="" data-description="" width="2048" height="883" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/683102739564180124_%E5%89%AF%E6%9C%AC_f1ny8y.png" class="" data-reactid="181"/></noscript></div></div></a><!-- react-text: 182 --> <!-- /react-text --><!-- react-text: 183 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="184"><span data-reactid="185"><div data-reactid="186"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="187"><div class=" columns sixteen" data-reactid="188"><div class="s-component s-text" data-reactid="189"><div class="s-component-content s-font-body" data-reactid="190"><p><em><strong>Fig.5 NGBC v0.1 dyed by different concentrations of purified EGFP protein solution</strong></em></p></div></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="191"><span data-reactid="192"><div data-reactid="193"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="194"><div class=" columns sixteen" data-reactid="195"><div class="s-component s-image" data-reactid="196"><span data-reactid="197"><div class="s-component-content" data-reactid="198"><div data-reactid="199"><a href="https://static.igem.org/mediawiki/2016/5/5e/T--BGIC_China--772887778940499081.jpg" target="_blank" data-reactid="200"><div class="s-ratio-box" style="max-width:2048px;max-height:1536px;" data-reactid="201"><div class="s-ratio-fill" style="padding-bottom:75.00%;" data-reactid="202"></div><div data-reactid="203"><img alt="" title="" data-description="" width="2048" height="1536" src="data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==" class="lazyload " data-src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/772887778940499081_cgor0m.jpg" data-sizes="auto" data-reactid="204"/><noscript data-reactid="205"><img alt="" title="" data-description="" width="2048" height="1536" src="//res.cloudinary.com/hrscywv4p/image/upload/c_limit,h_9000,w_1200,f_auto,q_90/v1/791914/772887778940499081_cgor0m.jpg" class="" data-reactid="206"/></noscript></div></div></a><!-- react-text: 207 --> <!-- /react-text --><!-- react-text: 208 --> <!-- /react-text --></div></div></span></div></div></div></div></span></div><div class="s-block-item s-repeatable-item s-block-sortable-item " data-reactid="209"><span data-reactid="210"><div data-reactid="211"><div class="s-block-item-inner clearfix" style="position:relative;" data-reactid="212"><div class=" columns sixteen" data-reactid="213"><div class="s-component s-text" data-reactid="214"><div class="s-component-content s-font-body" data-reactid="215"><p><em><strong>Fig.6 NGBC v0.1 dyed by different concentrations of purified EGFP protein solution</strong></em></p></div></div></div></div></div></span></div></div></div></div></div></li></ul><!-- react-empty: 216 --><div id="strikingly-tooltip-container" data-reactid="217"> </div><!-- react-empty: 218 --><!-- react-empty: 219 --><div class="s-floated-components" data-reactid="220"><div class="ecommerce-buy-dialog s-edit-modal extend" id="ecommerce-buy-dialog" data-reactid="221"><div class="s-async-wrapper s-component" data-reactid="222"><div class="s-loading" data-reactid="223"></div></div></div><div id="s-ecommerce-shopping-cart-wrapper" class="s-ecommerce-shopping-cart-wrapper" data-reactid="224"></div></div></div></div></div> <div id='fb-root'></div> <div id='app-script-root'></div> <div id='app-view-root'></div> <script id='blog-collection-tmpl' type='text/html'> <div class='s-blog-col-head'></div> 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Latest revision as of 19:25, 18 October 2016
NGBC prototype demonstration
NGBC v0.1
Fig.1 shows NGBC v0.1 which is the first prototype of our BioChips. We made the carrier of genetic circuits by filter paper manufactured by Whatman®. The black area of the filter paper is wax printed by wax-printer.
Fig.1 NGBC v0.1
Test for paper-based cell-free system
We first cutted the filter papers into small pieces of squares(3mm x 3mm) and placed them into wells of 384 well plates. 60ng of plasmid pCpxP-LacZ in 2ul of S30 cell-free system(pH = 7) and 400ng of X-gal (dissolved in DMSO; conc = 20ug/ul) was then added into each well and was immobilized onto filter papers by using freeze-dried technology. 2ul of PBS buffer solution(pH = 7) has then been added into each well to rehydrate the filter paper. After having reaction under 37℃ for 1 hour, we examined the result by looking at the colour of the filter paper. The change in colour of the filter paper demonstrated that the transcription and translation of β-galactosidase from LacZ gene had taken place (Fig.2 & Fig.3). Thus, it shows that the paper-based cell-free test paper works in laboratory condition.
Fig.2 Photo taken from 384 well plate. Left: Blank; Middle: Control; Right: pCpxP-LacZ immobilized filter paper.
Fig.3 Picture generated by using average color depth of each cell. Left: Blank; Middle: Control; Right: pCpxP-LacZ immobilized filter paper.
Supplementary pictures
Fig.4
Fig.5 NGBC v0.1 dyed by different concentrations of purified EGFP protein solution
Fig.6 NGBC v0.1 dyed by different concentrations of purified EGFP protein solution
