IrrE to aid E.coli growth in lube

First we performed a control experiment in which we grew wild-type E.coli in Lube of varying concentrations to see the natural tolerance E.coli has to lube

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We then wanted to see the exact concentration in which the growth is affected so repeated the experiment at smaller increments

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Once the IrrE part arrived we were able to repeat the above experiment with the transformed E.coli. This enabled us to compare WT E. coli with that of E.coli transformed with IrrE, a gene previously submitted to the registry which is shown to improve tolerance of E.coli to salt.

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