Difference between revisions of "Team:Exeter/Parts"
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<p id="pp">Here we are submitting lysozyme as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | <p id="pp">Here we are submitting lysozyme as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | ||
| − | <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and exchanged the native signal peptide for one which is <i>E. coli</i> specific and directs Lysozyme to the periplasm. The sequence for Lysozyme protein coding region can be found here (BBa_K1914004)</p> | + | <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and exchanged the native signal peptide for one which is <i>E. coli</i> specific and directs Lysozyme to the periplasm[5]. The sequence for Lysozyme protein coding region can be found here (BBa_K1914004)</p> |
<h6> Biobrick Code </h6> | <h6> Biobrick Code </h6> | ||
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<h6>Description:</h6> | <h6>Description:</h6> | ||
| − | <p id="pp"> DNase was designed as a DNA degrading kill switch aiming to prevent cross contamination of DNA</p> | + | <p id="pp"> DNase was designed [6] as a DNA degrading kill switch aiming to prevent cross contamination of DNA</p> |
| − | <p id="pp"> | + | <p id="pp"> .A composite part containing DNase could not be created This could be due to the need for a tightly regulating promoter to prevent DNase protein being produced before induction and destroying DNA.</p> |
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</li> | </li> | ||
<li>Blake, C.C.F., Koenig, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., 1965. Structure of hen egg-white lysozyme: a three-dimensional Fourier synthesis at 2 Å resolution. Nature, 206(4986), pp.757-761. | <li>Blake, C.C.F., Koenig, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., 1965. Structure of hen egg-white lysozyme: a three-dimensional Fourier synthesis at 2 Å resolution. Nature, 206(4986), pp.757-761. | ||
| + | </li> | ||
| + | <li>Fischer, B., Perry, B., Phillips, G., Sumner, I. and Goodenough, P., 1993. Physiological consequence of expression of soluble and active hen egg white lysozyme in Escherichia coli. Applied Microbiology and Biotechnology, 39, pp.537–540. | ||
| + | </li> | ||
| + | <li>Chen, C.Y., Lu, S.C. and Liao, T.H.,1998. Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli: purification and characterization of the recombinant enzyme. Gene, 206, pp.181–184. | ||
</li> | </li> | ||
</ol> | </ol> | ||
Revision as of 19:58, 18 October 2016
