Difference between revisions of "Team:Toronto/Notebook-w06-mon"
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</li> | </li> | ||
</ul> | </ul> | ||
| + | <li><a href="https://2016.igem.org/Team:Toronto/Achievements"><span>achievements</span></a></li> | ||
| + | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/Safety"><span>safety</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Safety"><span>safety</span></a></li> | ||
</li> | </li> | ||
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</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li> | ||
| + | </li> | ||
| + | <li><a href="https://2016.igem.org/Team:Toronto/HP-Impact"><span>impact</span></a></li> | ||
</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | ||
| − | |||
| − | |||
</li> | </li> | ||
</ul> | </ul> | ||
<li><a href="#"><span>awards</span></a> | <li><a href="#"><span>awards</span></a> | ||
<ul> | <ul> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<li><a href="https://2016.igem.org/Team:Toronto/Software"><span>software</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Software"><span>software</span></a></li> | ||
| − | |||
| − | |||
</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/Model"><span>model</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Model"><span>model</span></a></li> | ||
Revision as of 05:14, 19 October 2016
console.js:26
Monday June 20, 2016
Monday, 6/20
Members Present: Alex, Cathy, Kat, Hamed, Marc, Celene, Dk, Andrea
LAB:
Morning:
- Ran gel electrophoresis protocol using RFP and various amounts of DNA ladder found in the fridge (3ul, 6ul, 10ul) [picture to follow]
●
Concluded that 6uL of DNA ladder should be sufficient.
Afternoon:
Meeting with Dr. Pardee & Christian Euler
Administrative:
TO DO:
For the next day:
LAB TEAM:
LAB MANAGERS:
- Please buy a USB! (something cheap will do)
- Check the prices for the S30/S12 reagents (and if we have them in our inventory or the Biozone inventory):
●
ATP; GTP, UTP, CTP;
●
L-5-formyl-5, 6, 7, 8-tetrahydrofolic acid (folinic acid);
●
potassium glutamate;
●
ammonium glutamate;
●
magnesium glutamate;
●
20 amino acids (is there a mix?);
●
L-[14C(U)]-leucine;
●
nicotinamide adenine dinucleotide (NAD);
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coenzyme-A (CoA);
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spermidine;
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putrescine;
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sodium oxalate;
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phosphoenolpyruvate (PEP);
●
DTT
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NaH2PO4
●
Na2HPO4
A | B | C | D | E | |
1 | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
2 | Gel electrophoresis on RFP | Rubidium chloride competent cells | |||
3 | Restriction enzyme digest (using the iGEM protocol) | ||||
4 | Gel electrophoresis | ||||
Table1
Gmail correspondence:
Meetings/Notes:
Notes from General Meeting:
References:
