Difference between revisions of "Team:CU-Boulder/Attributions"

 
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<h3> <b> Undergraduates </b> </h3>
 
<h3> <b> Undergraduates </b> </h3>
 
<p class = "main">
 
<p class = "main">
−
All of the undergraduates executed all of the experiments. Each individual partook in creating the three plasmids necessary for the successful incorporation of the non-canonical amino acid. For the initial experiment, everyone helped demonstrate the localization of eGFP within the EutS compartments in various expression levels through molecular cloning. This was done in order to help improve the success rate of EutS compartmentalization. After this was proved, subteams were formed. </p>
+
All of the undergraduates executed all of the experiments. Each individual partook in creating the three plasmids necessary for the successful incorporation of the non-canonical amino acid. For the initial experiment, everyone helped demonstrate the localization of eGFP within the EutS compartments in various expression levels through molecular cloning. This was done in order to help improve the success rate of EutS compartmentalization. After this was proven, subteams were formed. </p>
  
  
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<tr><td>In order to successfully incorporate the non-canonical amino acid into this tri-plasmid system, genomic edits on the EutS genome must be made. Positions for mutagenesis was done in various spots on the genome in order to test whether or not the incorporation facilitated with the formation of the shell protein.Their primary tasks were to create a library of mutants with MUSE using CRISPR-Cas9. By making genomic edits, the native EutS shell protein was activated; also, variants created by these edits were used to test against the native EUTS compartment.</td></tr>
 
<tr><td>In order to successfully incorporate the non-canonical amino acid into this tri-plasmid system, genomic edits on the EutS genome must be made. Positions for mutagenesis was done in various spots on the genome in order to test whether or not the incorporation facilitated with the formation of the shell protein.Their primary tasks were to create a library of mutants with MUSE using CRISPR-Cas9. By making genomic edits, the native EutS shell protein was activated; also, variants created by these edits were used to test against the native EUTS compartment.</td></tr>
  
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<tr><td><b>Micheal Donovan:</b></tr></td>  
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<tr><td><b>Michael Donovan:</b></tr></td>  
 
<tr><td>Used PyRosetta position scoring to find possible binding sites of assembled hexamers. Looked at locations at the edge of EutS hexamer when aligned to E.coli EutM.</td></tr>
 
<tr><td>Used PyRosetta position scoring to find possible binding sites of assembled hexamers. Looked at locations at the edge of EutS hexamer when aligned to E.coli EutM.</td></tr>
 
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</table>
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<h3 style = "margin-top:640px"> <b> Co-Advisers </b> </h3>
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<h3 style = "margin-top:780px"> <b> Co-Advisers </b> </h3>
 
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<tr><td><b>Brian deDecker & Robin Dowell:</b></td></tr>
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<tr><td><b>Brian DeDecker & Robin Dowell:</b></td></tr>
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<tr><td>They handled the finances and written documents for the iGem team at CU Boulder.</td></tr>
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<tr><td>Handled the finances and written documents for the iGem team at CU Boulder.</td></tr>
 
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</table>
 
</div>
 
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<h2> Brian deDecker & Robin Dowell</h2>
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<h2> Brian DeDecker & Robin Dowell</h2>
 
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<p class = "main">
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They handled the finances and written documents for the iGem team at CU Boulder. </p>
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Handled the finances and written documents for the iGem team at CU Boulder. </p>
 
-->
 
-->
  
  
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<h3 style = "margin-top:210px"> <b> Grad-Students</b> </h3>
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<h3 style = "margin-top:210px"> <b> Mentors</b> </h3>
  
 
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<tr><td> <b> Michael Brasino: </b></td></tr>
 
<tr><td> <b> Michael Brasino: </b></td></tr>
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<tr><td>Oversaw the project; on a week-to-week basis, he helped provide insight on how to approach mishaps, guide us in the right direction for the experiments, and order lab material to conduct the experiments. He also helped create the azobenzene.</td></tr>
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<tr><td>Oversaw the project on a week-to-week basis, provided insight on how to approach mishaps and guided us in the right direction for the experiments. He also helped create the azobenzene.</td></tr>
 
<tr><td> <b> Marcelo Bassalo: </b></td></tr>
 
<tr><td> <b> Marcelo Bassalo: </b></td></tr>
 
<tr><td>Helped design the genomic Eut operon constructs to test the native system and generate the library variants. He provided the muse CREATE system using CRISPR-Cas9 to conduct the experiments.</td></tr>
 
<tr><td>Helped design the genomic Eut operon constructs to test the native system and generate the library variants. He provided the muse CREATE system using CRISPR-Cas9 to conduct the experiments.</td></tr>
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<h2> Michael Brasino</h2>
 
<h2> Michael Brasino</h2>
 
<p class = "main">
 
<p class = "main">
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Oversaw the project; on a week-to-week basis, he helped provide insight on how to approach mishaps, guide us in the right direction for the experiments, and order lab material to conduct the experiments. He also helped create the azobenzene. </p>
+
Oversaw the project on a week-to-week basis, provided insight on how to approach mishaps and guided us in the right direction for the experiments. He also helped create the azobenzene. </p>
  
 
<h2> Marcelo Bassalo</h2>
 
<h2> Marcelo Bassalo</h2>
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<h3 style = "margin-top:530px"> <b> Sponsors</b> </h3>
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<h3 style = "margin-top:630px"> <b> Sponsors</b> </h3>
  
 
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  <tr><td> <b> Muse: </b> </td></tr>
 
  <tr><td> <b> Muse: </b> </td></tr>
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<tr><td> Generated a library of EutS variants</td></tr>
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<tr><td> Generated a library of EutS variants
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<tr><td> <b> New England Biolab: </b> </td></tr>
+
 
−
<tr><td> Materials</td></tr>
+
<figure align="center">
 +
    <img src="https://static.igem.org/mediawiki/2016/8/89/T--CU-Boulder--musepsup.png" style="width:320px;height:196px; padding: 10px 10px 10px 10px;"></td></tr>
 +
 
 +
</td></tr>
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<tr><td> <b> New England Biolabs: </b> </td></tr>
 +
<tr><td> Materials
 +
<figure align="center">
 +
    <img src="https://static.igem.org/mediawiki/2016/2/2a/TCU-NEB.PNG" style="width:320px;height:96px; padding: 10px 10px 10px 10px;"></td></tr>
 
<tr><td><b>Integrated DNA technologies: </b></td></tr>
 
<tr><td><b>Integrated DNA technologies: </b></td></tr>
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<tr><td>Funding for oligomer purchases</td></tr>
+
<tr><td>Funding for oligomer purchases
−
<tr><td><b> Biofrontiers and IQ biology Program: </b></td></tr>
+
 
−
<tr><td>Funding</td></tr>
+
<figure align="center">
 +
    <img src="https://static.igem.org/mediawiki/2016/f/f3/TCU-Boulder-IDTdiff.PNG" style="width:320px;height:96px; padding: 10px 10px 10px 10px;"></td></tr>
 +
<tr><td><b> Biofrontiers: </b></td></tr>
 +
<tr><td>Funding
 +
<figure align="center">
 +
    <img src="https://static.igem.org/mediawiki/2016/e/e1/TCU-BioF.PNG" style="width:320px;height:96px; padding: 10px 10px 10px 10px;"></td></tr>
 +
 
 +
<tr><td><b>IQ biology Program: </b></td></tr>
 +
<tr><td>Funding
 +
 
 +
<figure align="center">
 +
    <img src="https://static.igem.org/mediawiki/2016/d/d5/TCU-IQBio.PNG" style="width:320px;height:96px; padding: 10px 10px 10px 10px;"></td></tr>
 +
 
 
<tr><td><b> Molecular Cellular and Developmental Biology Program: </b> </tr></td>
 
<tr><td><b> Molecular Cellular and Developmental Biology Program: </b> </tr></td>
−
<tr><td>Microscopy Time</td></tr>
+
<tr><td>Microscopy Time
 +
 
 +
<figure align="center">
 +
    <img src="https://static.igem.org/mediawiki/2016/6/6c/T--CU-Boulder--mcdbbio.png" style="width:300px;height:300px; padding: 10px 10px 10px 10px;"></td></tr>
 +
 
 +
</td></tr>
 
<tr><td><b> National Science Foundation </b></td></tr>
 
<tr><td><b> National Science Foundation </b></td></tr>
 +
<tr><td>Funding
 +
<figure align="center">
 +
    <img src="https://static.igem.org/mediawiki/2016/3/33/TCU-NSF.PNG" style="width:166px;height:166px; padding: 10px 10px 10px 10px;"></td></tr>
 
</table>
 
</table>
 
</div>
 
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Latest revision as of 05:23, 19 October 2016

Undergraduates

All of the undergraduates executed all of the experiments. Each individual partook in creating the three plasmids necessary for the successful incorporation of the non-canonical amino acid. For the initial experiment, everyone helped demonstrate the localization of eGFP within the EutS compartments in various expression levels through molecular cloning. This was done in order to help improve the success rate of EutS compartmentalization. After this was proven, subteams were formed.

Jonah, Brandon, Maxwell, and Jessica:
Their primary tasks were to create the desired tri-plasmid system; this included the EutS shell protein, EutC tagged with eGFP, and the pEVOL plasmid.
Elizabeth and Will:
In order to successfully incorporate the non-canonical amino acid into this tri-plasmid system, genomic edits on the EutS genome must be made. Positions for mutagenesis was done in various spots on the genome in order to test whether or not the incorporation facilitated with the formation of the shell protein.Their primary tasks were to create a library of mutants with MUSE using CRISPR-Cas9. By making genomic edits, the native EutS shell protein was activated; also, variants created by these edits were used to test against the native EUTS compartment.
Michael Donovan:
Used PyRosetta position scoring to find possible binding sites of assembled hexamers. Looked at locations at the edge of EutS hexamer when aligned to E.coli EutM.

Co-Advisers

Brian DeDecker & Robin Dowell:
Handled the finances and written documents for the iGem team at CU Boulder.

Mentors

Michael Brasino:
Oversaw the project on a week-to-week basis, provided insight on how to approach mishaps and guided us in the right direction for the experiments. He also helped create the azobenzene.
Marcelo Bassalo:
Helped design the genomic Eut operon constructs to test the native system and generate the library variants. He provided the muse CREATE system using CRISPR-Cas9 to conduct the experiments.
Peter Otoupal:
Helped coordinate funding and materials. Also participated in idea generation.

Sponsors

Muse:
Generated a library of EutS variants
New England Biolabs:
Materials
Integrated DNA technologies:
Funding for oligomer purchases
Biofrontiers:
Funding
IQ biology Program:
Funding
Molecular Cellular and Developmental Biology Program:
Microscopy Time
National Science Foundation
Funding