(11 intermediate revisions by the same user not shown) | |||
Line 6: | Line 6: | ||
<!-- repo for this wiki: https://github.com/igemuoftATG/wiki2016 --> | <!-- repo for this wiki: https://github.com/igemuoftATG/wiki2016 --> | ||
− | |||
− | |||
</html> | </html> | ||
{{Toronto/head}} | {{Toronto/head}} | ||
Line 15: | Line 13: | ||
<ul> | <ul> | ||
<li><a href="https://2016.igem.org/Team:Toronto"><span>home</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto"><span>home</span></a></li> | ||
+ | </li> | ||
+ | <li><a href="https://2016.igem.org/Team:Toronto/Achievements"><span>achievements</span></a></li> | ||
</li> | </li> | ||
<li><a href="#"><span>team</span></a> | <li><a href="#"><span>team</span></a> | ||
Line 62: | Line 62: | ||
</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li> | ||
+ | </li> | ||
+ | <li><a href="https://2016.igem.org/Team:Toronto/HP-Impact"><span>impact</span></a></li> | ||
</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | ||
− | |||
− | |||
</li> | </li> | ||
</ul> | </ul> | ||
<li><a href="#"><span>awards</span></a> | <li><a href="#"><span>awards</span></a> | ||
<ul> | <ul> | ||
− | |||
− | |||
− | |||
− | |||
<li><a href="https://2016.igem.org/Team:Toronto/Software"><span>software</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Software"><span>software</span></a></li> | ||
− | |||
− | |||
</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/Model"><span>model</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Model"><span>model</span></a></li> |
Latest revision as of 06:57, 19 October 2016
Monday June 13 2016
Monday, 6/13
Members Present: Celene, Hamed, Bohdan, Cathy, Dk, Alex, Kat, Davesh, Andrea
LAB:
Morning: LB prep + autoclaved 1.5mL microcentrifuge tubes
Afternoon:
- Carried out the iGEM transformation efficiency protocol
- 17 plates were plated with inoculating loops (three 0.5pg/uL, three 5pg/uL, three 10pg/uL, three 20pg/uL, three 50pg/uL and two negative controls using untransformed competent cells); and 5 or 6 plates were plated with glass beads (one for each concentration)
- Plates are placed in the broken shaker in WB403 for incubation at 37C
- NOTE for the future: when we want to count the colonies after the plates have been plated with cells, use glass beads instead of inoculating loops
- DH10B cell culture are placed in overnight shaker in the autoclave room
Administrative:
- Spoke to Susie regarding unknown waste, she will be combining this unknown solution with some of her solvents at a later date and dispose of it according to her judgement. The flask remains within 403, contained in a labled box, until this comes to pass.
- All remaining RRFs (Bohdan's and Celine's) have been approved and submitted to Susie.
-Formated "Order Log" found in google drive "Biomineralization" => "Inventory => "Order Log" and will be used to keep track of expenses and materials.
TO DO:
For the next day:
LAB TEAM:
LAB MANAGERS:
Gmail correspondence: Contacted advisors regarding weekly team meeting at WB 407 @ 4:00 pm
Meetings/Notes:
References: