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Latest revision as of 06:57, 19 October 2016
console.js:26
Week 2: May 27, 2016
Friday, 5/27
LynnMembers present: Celine, Tam, Hamed, Cathy, DK, Alex, Katariina
Lab Stuff: (research, plasmid design, protocol design, troubleshooting)
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Research into the appropriate promoter:
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"Transcription of gesABC is delayed compared with the Au-induced expression of golTS or golB, and requires higher GolS intracellular levels, suggesting a hierarchical activation of the gol regulon to cope with contaminated environments."
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In the presence of gold ions: There is a sensitivity/specificity trade-off between using pgolB and pges (http://www.ncbi.nlm.nih.gov/pubmed/17919284: see below)
Sensitivity:
Specificity:
In general, there is a high level of sensitivity to Au ions in pGolB than in pGesABC. However, pGesABC shows a higher specificity to Au ions than Cu ions in comparision to pGolB which can be induced by both Au and Cu ions.
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Creation of golS variants in the feature library using the following table to assist in optimizing the new codons:
Administrative: (shipment orders, inventory)
Email Updates: (Correspondance with proffessors, shipping companies, etc)
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Followed up regarding lockers
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Drafted e-mail to Kayla and sent to Umar for review before sending it out
Tasks to Follow up on - leading into week 3
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Promoter debacle (specificity/sensitivity) - All
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Luciferase/RFP (reaction with cellulose) - Kat
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merR family and Nickle sensors generally (as back up) - Wiki team
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Outlining Dr. Pardee's updated protocol - Kat
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golS structures (4 versions) - Tam
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118 - Kat
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113 - Alex
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113 + 118 - Alex
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CupR - Cathy
Meeting Notes:
Weekly meetings in WB 407, hopefully starting from the week that we are in the lab, this will be on Mondays at ? time
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We need a common medium of communication. – We decided on Facebook.
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We got the credit cards, which was given to DK/Zarifah. If you need to spend anything talk to Adele.
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PnP have yet to finish the synenergene project. They will now focus on heavy metal biosensing (as a environmental and health and safety concern), and bringing up wet lab and GolS as a proof-of-concept example. Furthermore, they will have Zika virus on the side as an example of an application for paper based biosensing methods.