Difference between revisions of "Team:Hong Kong UCCKE/Parts"

 
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                 <div>
 
                 <div>
 
                     <h1 data-toc-skip>UCCKE / Parts</h1>
 
                     <h1 data-toc-skip>UCCKE / Parts</h1>
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                     <p>offendit malis arbitror appellat o arbitror nisi nam quamquam illustriora do se appellat praetermissum.</p>
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                     <p>In this page, you can get an overall image of our parts</p>
 
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                 </div>
 
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             <div class="col-sm-9 col-xs-12">
 
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<h2 style="margin-top:0; padding-top:0;">Basic parts</h2>
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                <h2 style="margin-top:0; padding-top:0;">Safety Checklist</h2>
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                 <p style="text-align:left !important; font-size: 15px !important;">
 
                 <p style="text-align:left !important; font-size: 15px !important;">
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                     <ul style="list-style-position: outside; list-style-image: url('https://static.igem.org/mediawiki/2016/5/57/T--Hong_Kong_UCCKE--bullet-image.png'); font-size: 15px !important;">
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                     <table style="font-size:15px !important;">
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                        <li>Read about Safe Project Design, Safe Lab Work, and Safe Shipment</li>
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  <tr>
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                        <li>Notice the few hard-and-fast rules we have: don't use organisms from Risk Group 3 or 4, and don't release our project into the wild</li>
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    <th>Favourite</th>
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                        <li>Know our local guidelines: talk to our team instructors and our institution's biosafety officers</li>
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    <th>Name</th>
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                        <li>Ask for help: email safety (at) igem (dot) org, or join the iGEM team for Virtual Open Office Hours</li>
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    <th>Type</th>
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                    </ul>
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    <th>Description</th>
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    <th>Length</th>
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  </tr>
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  <tr>
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    <td>True</td>
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    <td><a href="http://parts.igem.org/Part:BBa_K1889006">BBa_K1889006</a></td>
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    <td>Coding</td>
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    <td>Protein Coding Sequence for Amino Acid Deaminase Gene</td>
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    <td>1620</td>
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  </tr>
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</table>
 
                 </p>
 
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             </div>
 
             </div>
 
             <div class="col-sm-4 col-xs-12">
 
             <div class="col-sm-4 col-xs-12">
 
                 <script src="https://2016.igem.org/Template:Hong_Kong_UCCKE/JS/bootstraptoc?action=raw&ctype=text/javascript" type="text/javascript"></script>
 
                 <script src="https://2016.igem.org/Template:Hong_Kong_UCCKE/JS/bootstraptoc?action=raw&ctype=text/javascript" type="text/javascript"></script>
 
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             </div>
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            <div class="col-sm-9 col-xs-12">
 
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                <h2>Safe Organisms</h2>
 
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                <p style="text-align:left !important;">We use two kinds of organisms, Escherichia coli and Caenorhabditis elegans in our experiments. E.coli is categorized as a Risk Group 1 organism <sup><a href="#ref1b" id="ref1a">[1]</a></sup> while C.elegans is on the whitelist <sup><a href="#ref2b" id="ref2a">[2]</a></sup>. According to the official iGEM description, “Risk Group 1 organisms do not cause disease in healthy adult humans.” and organisms on the whitelist “do not cause any threat to the environment”. To make sure that everything is safe enough to use, we have always been talking to our lab technician, Mr. Ho xx, about the proper usage, storage and disposal methods.</p>
 
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            <div class="col-sm-9 col-xs-12">
 
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                <h2>Safe Project Design</h2>
 
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                <p style="text-align:left !important;">Safety is always our first priority when carrying out the program. Although the only species we use is E.coli and C.elegans, which are only organisms which do not cause harm to human when used properly, we believe that there is still a danger when our bacteria is released into the public environment, especially to children and elderlies. For example, it may cause bacteria to develop antibiotics resistance and make more diseases incurable <sup><a href="#ref3b" id="ref3a">[3]</a></sup>. Therefore, we make sure that no bacteria or organism carrying our modified genes will be released out of the laboratory. To achieve this, the bacteria will always remain inside the laboratory; we always bring worms into the laboratory but not our bacteria outside the laboratory into the public environment. While doing any experiment with e.coli, we ensure the bacteria do not expose to the environment by setting up a sterile space using a Bunsen burner and working in the heated area. Also to keep the bacteria away from the open environment, we seal every used petri dishes with parafilm before long term storage.</p>
 
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            <div class="col-sm-4 col-xs-12"></div>
 
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            <div class="col-sm-9 col-xs-12">
 
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                <h2>Safe Lab Work</h2>
 
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                <p style="text-align:left !important;">Besides of ensuring a safe project design, we are solicitous about our biosafety and general safety in our lab work. All secondary schools use biology laboratories with biosafety level 1 <sup><a href="#ref4b" id="ref4a">[4]</a></sup>. We also believe that safe lab practices are important, so we emphasize safety through maintaining a high level of personal hygiene and apply good microbiology techniques <sup><a href="#ref5b" id="ref5a">[5]</a></sup>. We wear lab coats and gloves while doing any experiment and wash our hands with soap before leaving the laboratory. To prevent any bacteria being left on the bench after experiments, we always use alcohol to wipe the bench thoroughly before we leave. (While doing any experiment with e.coli, we ensure the bacteria do not expose to the environment by setting up a sterile space using a Bunsen burner and working in the heated area. Also to keep the bacteria away from the open environment, we seal every used petri dishes with parafilm before long term storage.) We received safety training when joining the team, topics include risk assessment, general precautions, fire precautions, handling chemical spills, disposal of chemical wastes and experiments on biotechnology. Details of the training can be found in <a href="http://cd1.edb.hkedcity.net/cd/science/laboratory/safety/SafetyHandbook2013_English.pdf">here</a>. which we follow to the letter.</p>
 
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            <div class="col-sm-9 col-xs-12">
 
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                <h2>Safe Shipment</h2>
 
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                <p style="text-align:left !important;">123</p>
 
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            <div class="col-sm-4 col-xs-12"></div>
 
  
 
             <div class="col-sm-9 col-xs-12" style="text-align:left !important;">
 
             <div class="col-sm-9 col-xs-12" style="text-align:left !important;">
  
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                <h2>Other Safety Concerns</h2>
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<h2 style="margin-top:0; padding-top:0;">Composite parts</h2>
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                <p style="text-align:left !important; font-size: 15px !important;">
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                    <table style="font-size:15px !important;">
 +
  <tr>
 +
    <th>Favourite</th>
 +
    <th>Name</th>
 +
    <th>Type</th>
 +
    <th>Description</th>
 +
    <th>Length</th>
 +
  </tr>
 +
  <tr>
 +
    <td>True</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1889007">BBa_K1889007</a></td>
 +
    <td>Composite</td>
 +
    <td>Express amino acid deaminase under constitutive promoter</td>
 +
    <td>1770</td>
 +
  </tr>
 +
  <tr>
 +
    <td></td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1889000">BBa_K1889000</a></td>
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    <td>Composite</td>
 +
    <td>Constitutive Promoter Expressing 4CL geneConstitutive Promoter Expressing PAL gene</td>
 +
    <td>1884</td>
 +
  </tr>
 +
  <tr>
 +
    <td></td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1889001">BBa_K1889001</a></td>
 +
    <td>Composite</td>
 +
    <td>Constitutive Promoter Expressing 4CL gene</td>
 +
    <td>1233</td>
 +
  </tr>
 +
  <tr>
 +
    <td></td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1889002">BBa_K1889002</a></td>
 +
    <td>Composite</td>
 +
    <td>Constitutive Promoter Expressing AtCCR1 gene</td>
 +
    <td>1422</td>
 +
  </tr>
 +
  <tr>
 +
    <td></td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1889021">BBa_K1889021</a></td>
 +
    <td>Composite</td>
 +
    <td>Constitutive Promoter Expressing PAL, 4CL and AtCCR1 gene</td>
 +
    <td>4903</td>
 +
  </tr>
 +
</table>
 +
                </p>
 +
             
  
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                <p style="text-align:left !important;">Some steps of our experiments require the use of UV light, whereupon exposure to UV radiation may result in sunburn, pigmentation of skin and slight discomfort <sup><a href="#ref7b" id="ref7a">[7]</a></sup>. To ensure our safety, we make sure that any parts of our body won’t be directly exposed to UV radiation; we will place a UV filter board <sup><a href="#ref8b" id="ref8a">[8]</a></sup> between us and the source of UV radiation so that it will be blocked from us.</p>
 
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<p style="margin-top:20px;"><span class="label label-primary" style="font-size:20px;">Reference</span></p>
 
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                <p id="ref1b" class="ref-text-left">
 
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<a href="#ref1a">[1]</a>: iGEM. (2016). Safety/Risk Groups. Available: https://2016.igem.org/Safety/Risk_Groups. Last accessed 26th Aug 2016.</p>
 
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<p id="ref2b" class="ref-text-left"><a href="#ref2a">[2]</a>: iGEM. (2016). Safety/White List. Available: https://2016.igem.org/Safety/White_List. Last accessed 26th Aug 2016.</p>
 
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<p id="ref3b" class="ref-text-left"><a href="#ref3a">[3]</a>: University of Minnesota. (2003). Genetically Modified Organisms (GMO). Available: http://enhs.umn.edu/current/5103/gm/harmful.html. Last accessed 26th Aug 2016.</p>
 
−
<p id="ref4b" class="ref-text-left"><a href="#ref4a">[4]</a>: World Health Organisation (2004). Laboratory Biosafety Manual. 3rd ed. Geneva: World Health Organisation. p2-3,9-19.</p>
 
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<p id="ref5b" class="ref-text-left"><a href="#ref5a">[5]</a>: World Health Organisation (2004). Laboratory Biosafety Manual. 3rd ed. Geneva: World Health Organisation. P69-93.</p>
 
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<p id="ref6b" class="ref-text-left"><a href="#ref6a">[6]</a>: World Health Organisation (2004). Laboratory Biosafety Manual. 3rd ed. Geneva: World Health Organisation. P94-95.</p>
 
−
<p id="ref7b" class="ref-text-left"><a href="#ref7a">[7]</a>: Paolo Vecchia, Maila Hietanen, Bruce E. Stuck Emilie van Deventer, Shengli Niu (2007). Protecting Workers from Ultraviolet Radiation. Germany: International Commission on Non-Ionizing Radiation Protection. P17-28.</p>
 
−
<p id="ref8b" class="ref-text-left"><a href="#ref8a">[8]</a>: Paolo Vecchia, Maila Hietanen, Bruce E. Stuck Emilie van Deventer, Shengli Niu (2007). Protecting Workers from Ultraviolet Radiation. Germany: International Commission on Non-Ionizing Radiation Protection. p65.</p>
 
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
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<h5>Safe Project Design</h5>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h5>Safe Lab Work</h5>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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</div>
 
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<div class="column half_size">
 
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<h5>Safe Shipment</h5>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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{{Hong_Kong_UCCKE/tourbefore|Notebook|Notebook|Basic Part|Basic Part}}
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{{Hong_Kong_UCCKE/heyfooter}}
 
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{{Hong_Kong_UCCKE/illbeback}}
 
{{Hong_Kong_UCCKE/illbeback}}

Latest revision as of 07:58, 19 October 2016

Basic parts

Favourite Name Type Description Length
True BBa_K1889006 Coding Protein Coding Sequence for Amino Acid Deaminase Gene 1620

Composite parts

Favourite Name Type Description Length
True BBa_K1889007 Composite Express amino acid deaminase under constitutive promoter 1770
BBa_K1889000 Composite Constitutive Promoter Expressing 4CL geneConstitutive Promoter Expressing PAL gene 1884
BBa_K1889001 Composite Constitutive Promoter Expressing 4CL gene 1233
BBa_K1889002 Composite Constitutive Promoter Expressing AtCCR1 gene 1422
BBa_K1889021 Composite Constitutive Promoter Expressing PAL, 4CL and AtCCR1 gene 4903