|
|
| Line 1: |
Line 1: |
| | {{OUC-China}} | | {{OUC-China}} |
| − | <html>
| |
| − | <meta charset="UTF-8">
| |
| − | <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
| |
| − | <meta http-equiv="X-UA-Compatible" content="IE=edge">
| |
| − | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/bootstrap-min?action=raw&ctype=text/css">
| |
| − | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/ouc_wiki?action=raw&ctype=text/css">
| |
| − | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/index?action=raw&ctype=text/css">
| |
| − | <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/jquery?action=raw&ctype=text/javascript"></script>
| |
| − | <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/bootstrap?action=raw&ctype=text/javascript"></script>
| |
| − | <title>Home</title>
| |
| − | <style type="text/css">
| |
| − | .banner-top{
| |
| − | margin-left: 12%;
| |
| − | }
| |
| − |
| |
| − | @media (min-width: 992px){
| |
| − | .work-content .col-md-4{
| |
| − | width:25%;
| |
| − | margin-right: 8%;
| |
| − | }
| |
| − |
| |
| − | }
| |
| − | #contentSub, #contentcontainer, #footer-box, #sitesub, #catlinks, #search-controls, #p-logo, .h3, .printfooter, .firstHeading, .visualClear {display: none;} /*-- hides default wiki settings --*/
| |
| − |
| |
| − | #globalWrapper, #content { /*-- changes default wiki settings --*/
| |
| − | width: 100%;
| |
| − | height: 100%;
| |
| − | border: 0px;
| |
| − | background-color: transparent;
| |
| − | margin: 0px;
| |
| − | padding: 0px;
| |
| − | }
| |
| − |
| |
| − | .oucBanner{background:url('https://static.igem.org/mediawiki/2016/e/ee/T--OUC-China--banner.fw.png');}
| |
| − | .oucNav{background:url('https://static.igem.org/mediawiki/2016/d/d0/T--OUC-China--nav-1.fw.png');}
| |
| − | ul[class=dropdown-menu]{background:url(https://static.igem.org/mediawiki/2016/d/d0/T--OUC-China--nav-1.fw.png);}
| |
| − |
| |
| − |
| |
| − | </style>
| |
| − | </head>
| |
| − | <body>
| |
| − | <div class="container">
| |
| − | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation">
| |
| − | <div class="container">
| |
| − | <div class="navbar-header">
| |
| − | <button type="button" class="navbar-toggle" data-toggle="collapse"
| |
| − | data-target="#example-navbar-collapse">
| |
| − | <span class="sr-only">Turn</span>
| |
| − | <span class="icon-bar"></span>
| |
| − | <span class="icon-bar"></span>
| |
| − | <span class="icon-bar"></span>
| |
| − | </button>
| |
| − | <a class="navbar-brand oucBrand" href="#"><img src="https://static.igem.org/mediawiki/2016/f/fc/T--OUC-China--nav-logo-1.fw.png" alt="Brand"></a>
| |
| − | </div>
| |
| − | <div class="collapse navbar-collapse" id="example-navbar-collapse">
| |
| − | <ul class="nav navbar-nav" id="oucNav">
| |
| − | <li class="active"><a href="https://2016.igem.org/Team:OUC-China">Home</a></li>
| |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a>
| |
| − | <ul class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Project">Introduction</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a>
| |
| − | <ul class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Parts">Parts</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Basic_Part">Basic part</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Composite_Part">Composite parts</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Part_Collection">Part collection</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a>
| |
| − | <ul class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a>
| |
| − | <ul class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a>
| |
| − | <ul class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
| |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a>
| |
| − | <ul class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
| |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − | </nav>
| |
| − | <div id="carousel-example-generic" class="carousel slide" data-ride="carousel">
| |
| − | <!-- Indicators -->
| |
| − | <ol class="carousel-indicators" style="left:20%;margin:0 auto;">
| |
| − | <li data-target="#carousel-example-generic" data-slide-to="0" class="active"></li>
| |
| − | <li data-target="#carousel-example-generic" data-slide-to="1"></li>
| |
| − | <li data-target="#carousel-example-generic" data-slide-to="2"></li>
| |
| − | <li data-target="#carousel-example-generic" data-slide-to="3"></li>
| |
| − | <li data-target="#carousel-example-generic" data-slide-to="4"></li>
| |
| − | </ol>
| |
| − |
| |
| − | <!-- Wrapper for slides -->
| |
| − | <div class="carousel-inner" role="listbox">
| |
| − | <div class="item active">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/5/5c/T--OUC-China--index-5.JPG" alt="img-1">
| |
| − | </div>
| |
| − | <div class="item">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/c/c1/T--OUC-China--index-2.JPG" alt="img-2">
| |
| − | </div>
| |
| − | <div class="item">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/2/2a/T--OUC-China--index-1.JPG" alt="img-3">
| |
| − | </div>
| |
| − | <div class="item">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/a/a2/T--OUC-China--index-3.JPG" alt="img-4">
| |
| − | </div>
| |
| − | <div class="item">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/5/54/T--OUC-China--index-4.JPG" alt="img-5">
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <!-- Controls -->
| |
| − | <a class="left carousel-control" href="#carousel-example-generic" role="button" data-slide="prev">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/5/50/T--OUC-China--index-left.png" style="margin-top:100%" alt="Slide Left">
| |
| − | <span class="sr-only"></span>
| |
| − | </a>
| |
| − | <a class="right carousel-control" href="#carousel-example-generic" role="button" data-slide="next">
| |
| − | <img src="https://static.igem.org/mediawiki/2016/f/f7/T--OUC-China--index-right.png" style="margin-top:100%" alt="Slide Right">
| |
| − | <span class="sr{{OUC-China}}
| |
| | <html> | | <html> |
| | <meta charset="UTF-8"> | | <meta charset="UTF-8"> |
| Line 302: |
Line 160: |
| | </div> | | </div> |
| | <div class="oucMovie" style="background:url('https://static.igem.org/mediawiki/2016/9/99/T--OUC-China--movie-1.fw.png');width:100%;height:200px;"> | | <div class="oucMovie" style="background:url('https://static.igem.org/mediawiki/2016/9/99/T--OUC-China--movie-1.fw.png');width:100%;height:200px;"> |
| − | <video src="https://static.igem.org/mediawiki/2016/d/de/T--OUC-China--Video1.mp4" controls="controls" width="80%" height="200px"> | + | <video src="https://static.igem.org/mediawiki/2016/d/de/T--OUC-China--Video1.mp4" controls="controls" width="80%" height="200"> |
| − | your browser does not support the video tag
| + | |
| − | </video>
| + | |
| − | </div>
| + | |
| − | <div class="oucMenu row">
| + | |
| − | <div class="col-xs-4">
| + | |
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2016/4/41/T--OUC-China--menu-icon-1.fw.png" class="img-responsive" alt="Purpose"><p class="text-center">Purpose</p></a>
| + | |
| − | </div>
| + | |
| − | <div class="col-xs-4">
| + | |
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--OUC-China--menu-icon-2.fw.png" class="img-responsive" alt="Principle"><p class="text-center">Principle</p></a>
| + | |
| − | </div>
| + | |
| − | <div class="col-xs-4">
| + | |
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2016/0/07/T--OUC-China--menu-icon-3.fw.png" class="img-responsive" alt="Potential"><p class="text-center">Potential</p></a>
| + | |
| − | </div>
| + | |
| − | <div class="clearfix"></div>
| + | |
| − | <div class="col-xs-4">
| + | |
| − | <a href="https://2016.igem.org/Team:OUC-China/Attributions"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--OUC-China--menu-icon-4.fw.png" class="img-responsive" alt="Attrbution"><p class="text-center">Attrbutions</p></a>
| + | |
| − | </div>
| + | |
| − | <div class="col-xs-4">
| + | |
| − | <a href="https://2016.igem.org/Team:OUC-China/Team"><img src="https://static.igem.org/mediawiki/2016/a/ad/T--OUC-China--menu-icon-5.fw.png" class="img-responsive" alt="Team"><p class="text-center">Team</p></a>
| + | |
| − | </div>
| + | |
| − | <div class="col-xs-4">
| + | |
| − | <a href="https://2016.igem.org/Team:OUC-China/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/0/04/T--OUC-China--menu-icon-6.fw.png" class="img-responsive" alt="Human Practices"><p class="text-center">Human Practices</p></a>
| + | |
| − | </div>
| + | |
| − | <div class="clearfix"></div>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | <div class="oucBanner">
| + | |
| − | <h3 class="text-center">Cistrons Concerto</h3>
| + | |
| − | </div>
| + | |
| − | <div class="row oucInformation">
| + | |
| − | <div class="col-md-1"></div>
| + | |
| − | <div class="col-md-5">
| + | |
| − | <h3>Thanks:</h3>
| + | |
| − |
| + | |
| − | <p><b>1.</b>Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
| + | |
| − | <p><b>2.</b>NEW ENGLAND Biolabs</p>
| + | |
| − | </div>
| + | |
| − | <div class="col-md-5 Contact">
| + | |
| − | <h3>Contact us:</h3>
| + | |
| − |
| + | |
| − | <p><b>E-mail</b>: oucigem@163.com</p>
| + | |
| − | <p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p>
| + | |
| − | <p>We are OUC-iGEM
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" width="70" height="70" alt="logo-one">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" width="70" height="70" alt="logo-two">
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="oucBottom">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | </body>
| + | |
| − | | + | |
| − | </html>
| + | |
| − | -only"></span>
| + | |
| − | </a>
| + | |
| − | </div>
| + | |
| − | <div class="oucAbstract row">
| + | |
| − | <div class="col-md-5">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--ab-icon1.png" class="img-responsive" alt="icon">
| + | |
| − | </div>
| + | |
| − | <div class="col-md-2">
| + | |
| − | <h3 class="text-center">ABSTRACT</h3>
| + | |
| − | </div>
| + | |
| − | <div class="col-md-5">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2016/d/d4/T--OUC-China--ab-icon2.png" class="img-responsive" alt="icon">
| + | |
| − | </div>
| + | |
| − | <div class="clearfix"></div>
| + | |
| − | <div class="text-center">
| + | |
| − | <p>As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, we can decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="oucMovie" style="background:url('https://static.igem.org/mediawiki/2016/9/99/T--OUC-China--movie-1.fw.png');width:100%;height:60%">
| + | |
| − | <video src="https://static.igem.org/mediawiki/2016/d/de/T--OUC-China--Video1.mp4" controls="controls" width="80%" height="60%" autoplay="autoplay">
| + | |
| | your browser does not support the video tag | | your browser does not support the video tag |
| | </video> | | </video> |
